Article
Biochemistry & Molecular Biology
Ryo Konno, Takashi Matsui, Hiroaki Ito, Yusuke Kawashima, Makoto Itakura, Yoshio Kodera
Summary: GeLC-MS/MS method coupled with stable isotope dimethyl labeling provides high accuracy and comprehensive peptide comparisons, enabling accurate determination of peptide abundance ratio between samples, unaffected by the reproducibility of gel extraction or LC-MS procedures, thus facilitating the detection of proteolysis events.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Xiaoling Su, Deying Chen, Liang Li, Lanjuan Li
Summary: The study demonstrates that perfusion can significantly alter the liver metabolome, recommending the use of non-perfused liver for comprehensive liver metabolomics analysis for high coverage.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Yan-Qing Chen, Hong Shen, Ru-Jie Yang, Jian-Bo Wan
Summary: A sensitive method was developed to accurately determine 20 NAEs in biological samples by chemical isotope labeling strategy coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Derivatized NAEs provided stable and abundant specific product ions in MS/MS spectrum for quantitation, enhancing detection sensitivity by 6- to 170-fold compared to non-derivatized NAEs. The method was successfully applied to determine NAEs in serum samples and liver tissues, showing practicability in analyzing endogenous NAE in biological samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Applied
Rui Chang, Xueting Wang, Yanxin Li, Shijuan Zhang
Summary: Two pairs of stable isotope labeling (SIL) agents were synthesized and their mass spectrometry (MS) sensitivity enhancement effect was compared with a commercial reagent. CPTA with quaternary ammonium group exhibited higher MS sensitivity enhancement effect and was successfully used for SIL analysis of alkylamines in food and food packaging materials. The SIL strategy and the permanent charge of CPTA minimized the matrix effect, and the method showed good limits of detection, quantitation, recoveries, and low matrix effect.
Article
Chemistry, Analytical
Kodai Beppu, Daichi Saito, Yoshio Muguruma, Miki Takahashi, Shuichi Harada, Koichi Inoue
Summary: This study developed a new stable isotope labeling method for analyzing residual antibiotics in bacteria culture, achieving acceptable recovery rates and representing a significant improvement over previous methods.
ANALYTICAL SCIENCES
(2021)
Article
Biochemical Research Methods
Sha Li, Yao-Yu Chen, Tian-Tian Ye, Quan-Fei Zhu, Yu-Qi Feng
Summary: A method combining chemical isotope labeling and LC-MS was developed for simultaneous determination of central carbon metabolism pathway (CCM) intermediates with high coverage and accuracy. By chemical derivatization, all CCM intermediates achieved better separation and accurate quantification in a single LC-MS run. This method was applied for accurate quantification of 22 CCM intermediates in different biological samples, and also for quantification at the single-cell level.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Pharmacology & Pharmacy
Sabrina Wohlfart, Michael Kilian, Philip Storck, Thomas Gutsmann, Klaus Brandenburg, Walter Mier
Summary: A novel method for precise quantification of Pep19-2.5 in biological samples has been developed in this study, which is crucial for the treatment of sepsis. The method utilizes mass spectrometry and labeled amino acid residues to reliably quantify the peptide content.
Article
Environmental Sciences
Eric N. Chiles, Ariana S. Huffmyer, Crawford Drury, Hollie M. Putnam, Debashish Bhattacharya, Xiaoyang Su
Summary: Corals form symbiotic relationships with dinoflagellate algae, bacteria, and other microbes, and nitrogen metabolism plays an important role in symbiotic regulation. Using stable isotope tracing, it was found that nitrogen assimilation in the coral holobiont may be compartmentalized, with different regions showing different nitrogen metabolism rates.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Zhihua Li, Ling Dong, Chi Zhao, Fengju Zhang, Shuang Zhao, Jingjing Zhan, Jia Li, Liang Li
Summary: This study presents a four-channel chemical isotope labeling (CIL) LC-MS approach for comprehensive metabolome analysis of high-salt fermented food. The method allows for high coverage identification and quantification of metabolites, revealing the high metabolic diversity in these foods.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemical Research Methods
Sha Li, Zheng Zhang, Fei-Long Liu, Bi-Feng Yuan, Tian-Gang Liu, Yu-Qi Feng
Summary: Phosphomonoester metabolites play a crucial role in microbial biosynthesis and energy metabolism. Researchers have developed a LC-MS method using isotope labeling for the comprehensive analysis of phosphomonoester metabolites. They successfully identified 42 phosphomonoester metabolites, including two that were first detected in Saccharomyces cerevisiae. The relative quantification results showed significant differences in the content of these metabolites between lycopene-producible and wild-type S. cerevisiae, and further experiments confirmed the relationship between lycopene production and the activity of a specific pathway.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemical Research Methods
Sha Li, Zheng Zhang, Fei-Long Liu, Bi-Feng Yuan, Tian-Gang Liu, Yu-Qi Feng
Summary: We developed an LC-MS method using isotope reagents for the comprehensive analysis of phosphomonoester metabolites. This method allowed for rapid screening, identification, and relative quantification of the metabolites in Saccharomyces cerevisiae. We identified 42 phosphomonoester metabolites, including two that were detected for the first time in S. cerevisiae. The relative quantification results revealed significant differences in certain metabolites between lycopene-producible and wild-type S. cerevisiae, indicating a close relationship between phosphomonoesters and lycopene production.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Food Science & Technology
Gbemisola J. Fadimu, Asgar Farahnaky, Harsharn Gill, Olusegun A. Olalere, Chee-Yuen Gan, Tuyen Truong
Summary: The use of natural ingredients for managing diabetes has become increasingly popular due to the adverse effects of synthetic antidiabetic medications. This study investigated the potential of hydrolysates from lupin proteins treated with ultrasound and hydrolyzed using alcalase and flavourzyme to inhibit alpha-glucosidase and alpha-amylase, enzymes involved in diabetes management. Peptide sequencing and molecular docking analysis identified specific peptides with potential antidiabetic activity. These findings suggest that hydrolysates from lupin proteins could be useful in formulating functional food for managing diabetes.
Article
Pharmacology & Pharmacy
Ju-Hsuan Huang, Nhung Thi Phuong Nong, Jue-Liang Hsu
Summary: In this study, a combination of ACE pre-incubation, LC-MS, and stable-isotope labeling was used for efficient screening of ACE's exogenous substrates from milk hydrolysate. 31 substrates were identified, and their activities were confirmed using synthetic peptides. The study also revealed the protective effects of AYFYPELFR and HLPLPLLQSW against ACE-mediated hydrolysis of Ang-I, suggesting their potential as substrate-type inhibitors.
Article
Biochemistry & Molecular Biology
Xian Luo, Liang Li
Summary: Cellular metabolomics provides insights into metabolic processes in cells, and this study investigates the effects of solvent evaporation equipment and storage conditions on high-coverage cellular metabolomics analysis. The choice of solvent evaporation equipment does not significantly impact the cellular metabolome, but the presence of methanol during storage can lead to notable changes in the metabolome after 7 days. Thoroughly drying cell samples before storage or shipment at room temperature is recommended to ensure high-quality results.
Article
Biochemical Research Methods
Molly Tillmann, Qian Tang, Jerry D. Cohen
Summary: The plant hormone auxin is crucial for plant growth and environmental response. Various pathways exist for the biosynthesis of IAA, the primary auxin, in different plant species. Techniques involving stable isotopes and chemical inhibitors are useful for tracing pathway utilization in vivo.
Article
Chemistry, Multidisciplinary
Wei-wei Liu, Fang Wang, Can Li, Xiao-yu Song, Wuxiyar Otkur, Yu-ying Zhu, Toshihiko Hayashi, Kazunori Mizuno, Shunji Hattori, Hitomi Fujisaki, Takashi Ikejima
Summary: The study demonstrates that silibinin can reduce UVB-induced skin cell apoptosis by inhibiting the YAP-p73 pathway.
ACTA PHARMACOLOGICA SINICA
(2022)
Article
Endocrinology & Metabolism
Tomoaki Saito, Masahiko Terajima, Yuki Taga, Fumihiko Hayashi, Sachi Oshima, Atsushi Kasamatsu, Yasuhiko Okubo, Chizuru Ito, Kiyotaka Toshimori, Masataka Sunohara, Hideki Tanzawa, Katsuhiro Uzawa, Mitsuo Yamauchi
Summary: This study demonstrates that significantly diminished levels of LH2 in LH2(+/-) mice result in decreased hydroxylation of lysine residues and collagen cross-linking in collagen, leading to reduced mineral densities and compromised mechanical properties in bones. These findings highlight the important role of LH2 in collagen molecular phenotype, bone matrix mineralization, and mechanical properties.
Article
Cell Biology
Yingying Zhu, Shuaigao Chen, Weiwei Liu, Fanxing Xu, Jingyu Lu, Toshihiko Hayashi, Kazunori Mizuno, Shunji Hattori, Hitomi Fujisaki, Takashi Ikejima
Summary: Inhibition of the IGF-1R/YAP signaling pathway is involved in collagen V-induced insulin biosynthesis and secretion in INS-1 cells.
CONNECTIVE TISSUE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Kazunori K. Fujii, Yuki Taga, Yusuke K. Takagi, Ryo Masuda, Shunji Hattori, Takaki Koide
Summary: Procollagen forms a triple helix structure in the endoplasmic reticulum with post-translational modifications enhancing its thermal stability. Collagen properties remain constant at lower temperatures, but at higher temperatures, an increase in post-translational modifications leads to higher thermal stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Masahiko Terajima, Yuki Taga, Tomoyuki Nakamura, Hou-Fu Guo, Yukako Kayashima, Nobuyo Maeda-Smithies, Kshitij Parag-Sharma, Jeong Seon Kim, Antonio L. Amelio, Kazunori Mizuno, Jonathan M. Kurie, Mitsuo Yamauchi
Summary: This study investigated the role of LH2 in collagen stability, organization, and mineralization using LH2-null MC3T3 cells. The results demonstrated that LH2 is involved in the hydroxylation of telopeptidyl Lys residues of collagen and affects collagen solubility, fibril diameter, and mineralization.
SCIENTIFIC REPORTS
(2022)
Article
Behavioral Sciences
Chenkang Wang, Yu Kang, Panwen Liu, Weiwei Liu, Wenhui Chen, Toshihiko Hayashi, Kazunori Mizuno, Shunji Hattori, Hitomi Fujisaki, Takashi Ikejima
Summary: Excessive physical exercise can result in damage to vital tissues, including the hippocampus, which is important for learning and memory. A combination of dasatinib and quercetin, known as senolytic drugs, selectively kill senescent cells. In this study, rats subjected to excessive swimming training were treated with dasatinib and quercetin, which improved exercise performance and prevented deficits in learning and memory. The drugs reduced senescent cells and apoptosis in the hippocampus, ultimately alleviating the negative effects of overtraining.
BEHAVIOURAL BRAIN RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Yoshihiro Ishikawa, Yuki Taga, Thibault Coste, Sara F. Tufa, Douglas R. Keene, Kazunori Mizuno, Elisabeth Tournier-Lasserve, Douglas B. Gould
Summary: Collagens are important proteins in the body, and mutations in genes encoding them can cause connective tissue disorders. Lysyl hydroxylase 3 (LH3) plays a critical role in collagen biosynthesis, and LH3 pathogenic variants may contribute to Gould syndrome.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Plant Sciences
Panwen Liu, Chenkang Wang, Wenhui Chen, Yu Kang, Weiwei Liu, Zhiyue Qiu, Toshihiko Hayashi, Kazunori Mizuno, Shunji Hattori, Hitomi Fujisaki, Takashi Ikejima
Summary: Silibinin, a natural flavonoid from Silybum marianum seeds, has neuroprotective effects on sporadic Alzheimer's disease models. It exerts its effects by inhibiting over-activation of N-methyl-D-aspartate receptors (NMDARs).
Article
Biochemistry & Molecular Biology
Can Li, Yuying Zhu, Weiwei Liu, Toshihiko Hayashi, Wendie Xiang, Sijun He, Kazunori Mizuno, Shunji Hattori, Hitomi Fujisaki, Takashi Ikejima
Summary: UVB irradiation induces skin inflammation and apoptosis. Mitochondrial dynamics play a crucial role in these processes. Inhibiting mitochondrial fission prevents UVB-induced inflammation and apoptosis, while inhibiting mitochondrial fusion increases these processes. Treatment with an antioxidant, NAC, can reduce inflammation and apoptosis, providing a new therapy for UVB skin injury.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Can Li, Yuying Zhu, Weiwei Liu, Wendie Xiang, Sijun He, Toshihiko Hayashi, Kazunori Mizuno, Shunji Hattori, Hitomi Fujisaki, Takashi Ikejima
Summary: This study focuses on the involvement of mitochondrial disorders in UVB-induced skin damages. UVB irradiation increases the amount of dysfunctional mitochondria in HaCaT cells, leading to decreased ATP production, mitochondrial membrane potential collapse, and apoptosis. The impairment of PINK1/parkin-mediated mitophagy contributes to UVB-induced cell damages by activating cGAS-STING pathway and promoting caspase-3-mediated apoptosis. Restoring mitophagy may be a potential strategy to protect skin from UVB damages.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Nutrition & Dietetics
Kumiko Kuwaba, Masashi Kusubata, Yuki Taga, Hiroshi Igarashi, Koichi Nakazato, Kazunori Mizuno
Summary: This study investigated the effects of dietary collagen peptides (CPs) on post-exercise physical condition and fitness in healthy middle-aged adults unfamiliar with exercise. The results showed that dietary CPs can alleviate muscle soreness and fatigue, and improve muscle strength.
JOURNAL OF THE INTERNATIONAL SOCIETY OF SPORTS NUTRITION
(2023)
Proceedings Paper
Computer Science, Artificial Intelligence
Koichiro Sato, Kazunori Mizuno
Summary: This paper proposes a method for emergently generating complex camouflage patterns using an Ant Painting algorithm. By extracting features of an input image, artificial ants draw a camouflage according to these features. It is demonstrated that the proposed method can generate complex camouflage patterns that blend better with the background image through interaction among ants.
2022 INTERNATIONAL CONFERENCE ON TECHNOLOGIES AND APPLICATIONS OF ARTIFICIAL INTELLIGENCE, TAAI
(2022)
Proceedings Paper
Computer Science, Artificial Intelligence
Yosuke Suzuki, Kazunori Mizuno
Summary: This study proposes a two-phase optimization method for scheduling teachers and students, using genetic algorithms for employee shift scheduling and simulated annealing for student timetabling.
2022 INTERNATIONAL CONFERENCE ON TECHNOLOGIES AND APPLICATIONS OF ARTIFICIAL INTELLIGENCE, TAAI
(2022)
Article
Food Science & Technology
Yuki Taga, Yu Iwasaki, Chisa Tometsuka, Noriko Funato, Yasutaka Shigemura, Masashi Kusubata, Kazunori Mizuno
Summary: Studies have found that Gly-3Hyp-4Hyp tripeptide can be detected in human blood at high concentrations after ingestion of collagen hydrolysate, and it exhibits stability and resistance to degradation enzymes. This tripeptide has potential for health promotion and disease treatment.
NPJ SCIENCE OF FOOD
(2022)
