Article
Chemistry, Applied
Linlin Huang, Jing Zhao, Yunlu Wei, Guoyong Yu, Fei Li, Quanhong Li
Summary: CMDP-4b, a pectic polysaccharide extracted from Cucurbita moschata Duch, possesses immunomodulatory activity by inducing nitrous oxide and cytokine secretion, as well as promoting macrophage activity through the NFκB and MAPKs signaling pathways. Toll-like receptor 4 and complement receptor 3 may play a critical role in CMDP-4b-induced macrophage activation.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Multidisciplinary
David Szamosvari, Munhyung Bae, Sunghee Bang, Betsabeh Khoramian Tusi, Chelsi D. Cassilly, Sung-Moo Park, Daniel B. Graham, Ramnik J. Xavier, Jon Clardy
Summary: This article explains what the immunogens for Lyme disease are, and points out the importance of making headgroup structural changes to the immunogens for the diagnosis, prevention, and treatment of the disease.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Peipei Li, Junlu Bai, XiaoJun Zhang, Zhongyong Yan, Pengfei He, Yin Chen
Summary: A water-soluble sulfated polysaccharide, F2-1, was extracted from the marine green alga Codium isthmocladum. The polysaccharide showed significant anticoagulant activity and can be considered as a potential bioactive polysaccharide resource.
Article
Biochemistry & Molecular Biology
Linlin Huang, Jing Zhao, Yunlu Wei, Guoyong Yu, Quanhong Li
Summary: A neutral polysaccharide CMDP-1a was isolated from pumpkin, with a determined structure of alpha-1,4 linked glucopyranosyl residues. This polysaccharide showed potential immunomodulatory effects and could be used as a food immunomodulator.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Applied
Yin Chen, Teng Wang, Xing Zhang, Fuming Zhang, Robert J. Linhardt
Summary: A neutral branched heteropolysaccharide Pc0-1 was isolated from the spores of Paecilomyces cicadae parasitizing bamboo cicadas. Pc0-1 has a core structure with glucose, galactose, mannose, and arabinose residues, showing moderate immunostimulating activity by activating macrophages through their mannose receptor (MR) and involving TLR4 and TLR2 recognition.
CARBOHYDRATE POLYMERS
(2021)
Article
Biochemistry & Molecular Biology
QingChi Wang, Maosheng Wei, Jingjing Zhang, Yang Yue, Ning Wu, Lihua Geng, Chaomin Sun, Quanbin Zhang, Jing Wang
Summary: EPS2E1, an exopolysaccharide isolated from cold-seep bacterium Halomonas sp. 2E1, exhibits immune-enhancing activity by activating MAPK and NF-KB pathways in RAW264.7 macrophages. It contains mannose and glucose, and has the potential to be an immunopotentiator in the future.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Gregory S. Bulmer, Fang Wei Yuen, Naimah Begum, Bethan S. Jones, Sabine L. Flitsch, Jolanda M. van Munster
Summary: In this study, a galactofuranosidase from the fungus Aspergillus niger was identified and characterized. Analysis of glycoside hydrolase family 43 subfamily 34 (GH43_34) members revealed similar enzyme clustering with characterized bacterial enzymes. Through molecular docking and enzymatic studies, a Galf-specific galactofuranosidase was identified in A. niger. This study provides molecular insight into the degradation mechanism of Galf-containing structures.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Huihui Sun, Fang Shu, Yue Guan, Fange Kong, Shuyan Liu, Yang Liu, Lanzhou Li
Summary: In this study, a polysaccharide (AGP) was extracted and purified from Armillaria gallica. The structural characterization of AGP was performed, including analysis of homogeneity, molecular weight and monosaccharide composition. AGP exhibited anti-fatigue effects in a mouse model of acute fatigue, improving exercise endurance and reducing fatigue symptoms. AGP also regulated various biochemical parameters related to fatigue, affected the composition of intestinal microbiota, and modulated oxidative stress levels through the AMP-dependent protein kinase/nuclear factor erythroid 2-related factor 2 signaling pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Food Science & Technology
He Liu, Jiaxin Xu, Xinyue Xu, Zhiheng Yuan, Hong Song, Lina Yang, Danshi Zhu
Summary: This review provides a foundation for future research and development of bean polysaccharides. It summarizes the sources, structure, and functions of bioactive bean polysaccharides, focusing on their role in immunology, antioxidant, and anti-diabetic activities. The article offers useful guidance for optimizing polysaccharide structure and developing bean polysaccharides as novel functional materials.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Medicine, Research & Experimental
Jiatong Zhang, Jingwen Cui, Jiafeng Gao, Di Zhang, Degui Lin, Jiahao Lin
Summary: Based on the experimental results, it was found that plantain polysaccharide (PLP) extracted from Plantago asiatica has the ability to polarize macrophages and inhibit the growth of breast tumors by promoting immune system function. The results showed that PLP could indirectly inhibit the migration of breast tumor cells by enhancing macrophages' phagocytosis and release of reactive oxygen species (ROS). Additionally, PLP could also promote the accumulation of macrophages and T cells in the spleen and lymph node, effectively inhibiting the growth of breast tumors.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Chemistry, Applied
Yoong Kit Leong, Fan-Chiang Yang, Jo-Shu Chang
Summary: Mushroom-derived polysaccharides, especially beta-glucans, have gained significant interest for their antioxidant, antitumor, immune-modulating properties and other health benefits. A variety of advanced extraction technologies are now available for recovering these bioactive ingredients, each with its own strengths and limitations.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Physical
Changbiao Chi, Run Xu, Qianqian Chen, Xiaohui Zhang, Xiaomeng Shi, Hongwei Jin, Fuling Yin, Hongli Jia, Liangren Zhang, Donghui Yang, Jianhua Ju, Qinglian Li, Ming Ma
Summary: In this study, the crystal structure of metal-dependent mutase MtdL is reported, which shows poor similarity to other known enzymes. Molecular dynamics simulations, site-directed mutagenesis, and proteomics analysis were used to identify key catalytic residues and support an Arg159-covalently mediated catalytic mechanism. Sequence alignment reveals conserved key residues in other metal-dependent mutases, suggesting a common catalytic paradigm. These results enhance our understanding and facilitate the use of metal-dependent mutases in the generation of furanose-containing molecules.
Article
Cell Biology
Oleg V. Chernikov, Hsiao-Wen Chiu, Lan-Hui Li, Maxim S. Kokoulin, Valentina I. Molchanova, Hsien-Ta Hsu, Chen-Lung Ho, Kuo-Feng Hua
Summary: Polysaccharides from marine organisms, such as PPA from Pseudopterogorgia americana, have immunomodulatory effects on mammalian immune system by activating macrophages and inducing cytokine expression through pathways involving ROS, PKC, MAPK, and NF-κB signaling. This study provides insights into the potential development of PPA as immune modulators in the future.
Article
Biochemistry & Molecular Biology
Jun-Geon Je, Hyo-Geun Lee, Kurukulasuriya H. N. Fernando, You-Jin Jeon, Bomi Ryu
Summary: In this study, sulfated polysaccharides were extracted from brown algae, Sargassum binderi, purified, and characterized for their biological activities. Among the tested fractions, SBP-F4 exhibited the highest polysaccharide and sulfate contents with significant suppressive effects on the expression of iNOS and COX-2 proteins in macrophages. Furthermore, in a zebrafish model, SBP-F4 showed a decrease in cell death and NO production, indicating its potential as an anti-inflammatory agent.
Article
Pharmacology & Pharmacy
Ming Wu, Hongrui Ping, Kun Wang, Hui Ding, Meng Zhang, Ziqiong Yang, Qian Du
Summary: Oral delivery of anti-inflammatory drugs is a promising strategy for treating ulcerative colitis, but achieving specific drug delivery to colon tissues and target cells is challenging. In this study, macrophages-targeted liposome-loaded pectin-chitosan hydrogels were developed for effective UC treatment through oral administration.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)