Article
Biochemistry & Molecular Biology
Yu-Jen Chen, Chia-Tien Hsu, Shang-Feng Tsai, Cheng-Hsu Chen
Summary: Chronic allograft dysfunction (CAD) is a major condition affecting long-term kidney graft survival. In this study, the correlation between plasma miR-21 and the severity of CAD was investigated. RNA sequencing was performed and differentially expressed miRNAs in CAD were identified. MiR-21-5p, miR-20a-5p, and miR-101-3p were found to be associated with the TGF-beta pathway and CAD. These findings may lead to the development of early prediction miRNA biomarkers and therapeutic tools for CAD in the field of kidney transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Clinical Neurology
Rukiye Tekdemir, Yavuz Selvi, Kursat Altinbas, Nadir Kocak
Summary: This study found that miRNAs play an important role in the pathogenesis of bipolar disorder and may serve as biomarkers for diagnosis and screening of the disorder.
JOURNAL OF AFFECTIVE DISORDERS
(2022)
Article
Immunology
Tan Li, Jingjing Jing, Liping Sun, Yuehua Gong, Jun Yang, Chunyan Ma, Yuan Yuan
Summary: This study found that SNPs in pri-miR-1-3p are associated with increased risk of abdominal aortic aneurysm (AAA), particularly the rs4591246 variant genotype. The expression of miR-1-3p is reduced in AAA patients. Further investigation revealed that the rs4591246 polymorphism affects cell phenotypic transformation through the miR-1-3p/TLR4 axis, suggesting its potential as a genetic biomarker for AAA susceptibility.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Andrology
Yi Ma, Yan Zhou, Qian Xiao, Sha-Sha Zou, Yin-Ci Zhu, Ping Ping, Xiang-Feng Chen
Summary: The study found that seminal exosomal miR-210-3p levels were positively correlated with the severity of varicocoele in patients, while negatively correlated with sperm count and seminal inhibin-B expression. Surgical treatment significantly reduced the level of miR-210-3p.
Article
Oncology
Huangkai Zhu, Xiang Xu, Enkuo Zheng, Junjun Ni, Xu Jiang, Minglei Yang, Guofang Zhao
Summary: This study aimed to elucidate the molecular mechanism of lncRNA RP11-805J14.5 in LUAD development. The study found that RP11-805J14.5 was highly expressed in LUAD tissues and cells and was associated with poor prognosis in LUAD patients. Knockdown of RP11-805J14.5 suppressed LUAD cell growth, invasion, migration, and tumor growth, indicating that RP11-805J14.5 is an important regulator in LUAD.
Article
Oncology
Yoko Hagihara, Yuya Tomioka, Takayuki Suetsugu, Masahiro Shinmura, Shunsuke Misono, Yusuke Goto, Naoko Kikkawa, Mayuko Kato, Hiromasa Inoue, Keiko Mizuno, Naohiko Seki
Summary: Analyses of miRNA expression signature combined with TCGA data revealed the downregulation of both strands of miR-139 in LUAD clinical specimens. Overexpression of miR-139-3p suppressed the aggressiveness of LUAD cells. TRIP13, a target gene of miR-139-3p, was found to promote cancer in LUAD cells.
Article
Physiology
Jian Han, Xiaogang Cui, Tianqi Yuan, Zhiming Yang, Yue Liu, Yajuan Ren, Changxin Wu, Yunfei Bian
Summary: Circulating exosomal miRNAs have potential as biomarkers for diagnosis of cardiovascular diseases. In this study, the differentially expressed miRNAs in exosomes from plasma of SCAD patients were analyzed and their diagnostic potential for SCAD was investigated.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Health Care Sciences & Services
Yi Wang, Yanzhi Zou, Qingmei Zhang, Defu Chen, Lin Lin
Summary: The study found that SBF2-AS1 is highly expressed in NSCLC, while miR-520a-3p expression is decreased. SBF2-AS1 promotes the proliferation, migration, and invasion of NSCLC cells by regulating miR-520a-3p. High expression of SBF2-AS1 is associated with poor overall survival in NSCLC patients.
JOURNAL OF HEALTHCARE ENGINEERING
(2022)
Article
Multidisciplinary Sciences
Guolei Cao, Peiwen Fan, Ronghui Ma, Qinghe Wang, Lili He, Haiwen Niu, Qin Luo
Summary: This study investigated the role of microRNA-210 (miR-210) in lung adenocarcinoma (LUAD). The expression levels of miR-210 and its target genes were analyzed in LUAD tissues and normal tissues. It was found that miR-210 inhibited the expression of HIF-1a and VEGF, leading to decreased apoptosis and worse patient survival. These findings suggest that miR-210 could be a potential therapeutic target for LUAD treatment.
Article
Biochemistry & Molecular Biology
Shalini Das Gupta, Robert Ciszek, Mette Heiskanen, Niina Lapinlampi, Janne Kukkonen, Ville Leinonen, Noora Puhakka, Asla Pitkanen
Summary: The study identified plasma miR-9-3p and miR-136-3p as promising biomarker candidates for mTBI, showing potential for distinguishing mTBI patients with high sensitivity and specificity. Further validation in a larger patient population is needed to confirm their diagnostic utility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Yuya Tomioka, Takayuki Suetsugu, Naohiko Seki, Kengo Tanigawa, Yoko Hagihara, Masahiro Shinmura, Shunichi Asai, Naoko Kikkawa, Hiromasa Inoue, Keiko Mizuno
Summary: Two microRNAs (miR-486-5p and miR-486-3p) derived from pre-miR-486 act as tumor-suppressive miRNAs in lung adenocarcinoma (LUAD). These miRNAs coordinately regulate seven genes (MKI67, GINS4, RRM2, HELLS, MELK, TIMELESS, and SAPCD2) involved in the malignant phenotype of LUAD cells. Controlling these genes may suppress the malignant transformation of LUAD.
Article
Oncology
Kelin She, Shaoqi Yu, Shushuai He, Wen Wang, Biao Chen
Summary: This study found that circ_0009043 is upregulated in NSCLC cells and tissues, and lower expression of circ_0009043 is associated with poorer overall survival in NSCLC patients. Mechanistically, circ_0009043 acts as a molecular sponge, sequestering miR-148a-3p and promoting the expression of DNAJB4, thereby acting as a tumor suppressor in NSCLC.
BIOMARKER RESEARCH
(2022)
Article
Oncology
Jin Piao, John T. Lafin, Cinzia G. Scarpini, Michelle M. Nuno, Isabella Syring, Klaus-Peter Dieckmann, Gazanfer Belge, Joerg Ellinger, James F. Amatruda, Aditya Bagrodia, Nicholas Coleman, Mark D. Krailo, A. Lindsay Frazier, Matthew J. Murray
Summary: The study demonstrates that quantifying circulating miR-371a-3p alone is sufficient for diagnosing testicular cancer, and adding other three microRNAs to the measurement does not provide additional diagnostic benefit.
CLINICAL GENITOURINARY CANCER
(2021)
Article
Biochemistry & Molecular Biology
Keiko Mizuno, Kengo Tanigawa, Shunsuke Misono, Takayuki Suetsugu, Hiroki Sanada, Akifumi Uchida, Minami Kawano, Kentaro Machida, Shunichi Asai, Shogo Moriya, Hiromasa Inoue, Naohiko Seki
Summary: Studies have shown that both strands of certain miRNAs derived from miRNA duplexes are involved in cancer pathogenesis, with miR-150-3p acting as a tumor-suppressive miRNA in lung squamous cell carcinoma by targeting cell cycle-related genes. Direct control of HELLS expression by miR-150-3p promotes the malignant phenotype of LUSQ cells.
Article
Oncology
Rocio B. Duca, Cintia Massillo, Guillermo N. Dalton, Paula L. Farre, Karen D. Grana, Kevin Gardner, Adriana De Siervi
Summary: This research identified the association between a high fat diet and prostate cancer, pinpointing the potential importance of two miRNAs in the diagnosis and prognosis of prostate cancer. Further investigations revealed the significant relevance of these miRNAs in distinguishing tumor types and metastatic status.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Article
Health Care Sciences & Services
Michal Seweryn Karbownik, Robert Horne, Ewelina Paul, Edward Kowalczyk, Janusz Szemraj
Summary: Based on the study results, Polish internet users with no medical education exhibit some false beliefs regarding dietary supplements. Trust in advertising conflicts with knowledge about them. There is an urgent need to conduct effective web-based educational campaigns and promote advertising literacy.
JOURNAL OF MEDICAL INTERNET RESEARCH
(2021)
Article
Public, Environmental & Occupational Health
Michal Seweryn Karbownik, Maria Dobielska, Ewelina Paul, Radoslaw Przemys law Kowalczyk, Edward Kowalczyk
Summary: The study found that during the COVID-19 pandemic lockdown, most examined outcomes remained unchanged, but beliefs in the quality control of dietary supplements significantly increased. Fewer people reported having contact with dietary supplement advertisements. These results may help in understanding health-related issues during the COVID-19 pandemic lockdown and shaping health-related policy.
RESEARCH IN SOCIAL & ADMINISTRATIVE PHARMACY
(2021)
Article
Environmental Sciences
Mateusz Kowalczyk, Michal Seweryn Karbownik, Edward Kowalczyk, Monika Sienkiewicz, Monika Talarowska
Summary: The study found that there are mental health issues among PhD students, especially in terms of anxiety, insomnia, social dysfunction, and somatic symptoms. Depression is more common among individuals without relationships, anxiety and insomnia are more common in women compared to men, and these issues are more prevalent in technical university PhD students than those in general universities.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Article
Endocrinology & Metabolism
Michal Seweryn Karbownik, Joanna Kreczynska, Anna Wiktorowska-Owczarek, Paulina Kwarta, Magdalena Cybula, Nebojsa Stilinovic, Tadeusz Pietras, Edward Kowalczyk
Summary: This study aimed to investigate the effects of supplementation with Saccharomyces boulardii on salivary serotonin levels under psychological stress. The results showed that Saccharomyces boulardii supplementation decreased salivary serotonin concentration compared to placebo. Salivary serotonin was positively correlated with sympathetic-adrenal markers.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell Biology
Rafal Szelenberger, Michal Seweryn Karbownik, Michal Kacprzak, Karina Maciak, Michal Bijak, Marzenna Zielinska, Piotr Czarny, Tomasz Sliwinski, Joanna Saluk-Bijak
Summary: Transcriptome analysis is an important method to understand the genetic basis of disease pathogenesis, with microRNAs playing a key role in post-transcriptional gene expression regulation. In this study, platelet microRNA profiles were used to identify potential biomarkers for acute coronary syndrome (ACS), with eight overexpressed microRNAs associated with platelet function. A combined model using miR-142-3p and aspartate transaminase showed high sensitivity and specificity in differentiating study groups. Cluster analysis revealed two groups of microRNAs regulated by different biological factors, potentially linked to platelet physiological processes and interactions with the vascular environment. This research contributes to a better understanding of the role of platelet microRNAs in ACS pathophysiology and risk modeling.
Article
Environmental Sciences
Michal Seweryn Karbownik, Lukasz Mokros, Edward Kowalczyk
Summary: Probiotic therapies and fermented food diets may improve mental health, especially for individuals with psychiatric illnesses. However, consuming more fermented food may result in more severe depressive and anxiety symptoms for mentally healthy individuals.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Psychiatry
Michal Seweryn Karbownik, Steven Daniel Hicks
Summary: This study found a negative association between salivary serotonin concentration and current mood, but no significant association with cardiovascular and autonomic parameters.
FRONTIERS IN PSYCHIATRY
(2022)
Article
Biology
Rafal Szelenberger, Michal Seweryn Karbownik, Michal Kacprzak, Ewelina Synowiec, Sylwia Michlewska, Michal Bijak, Marzenna Zielinska, Alina Olender, Joanna Saluk-Bijak
Summary: This study aims to detect potential changes at the molecular level in platelet surface receptors that could be of potential importance in the occurrence of Acute Coronary Syndrome. The results show that the P2Y12 receptor is expressed more frequently among patients, and there are quantitative changes in other platelet receptors important in platelet activation at the genetic level.
Article
Nutrition & Dietetics
Michal Seweryn Karbownik, Lukasz Mokros, Maria Dobielska, Mateusz Kowalczyk, Edward Kowalczyk
Summary: Consumption of fermented food and food-derived prebiotics is not associated with cognitive performance in mentally healthy medical students. However, high intake of fermented food may be linked to the severity of depressive and anxiety symptoms.
FRONTIERS IN NUTRITION
(2022)
Article
Nanoscience & Nanotechnology
Agnieszka M. Pudlarz, Katarzyna Ranoszek-Soliwoda, Michal S. Karbownik, Ewa Czechowska, Emilia Tomaszewska, Grzegorz Celichowski, Jaroslaw Grobelny, Ewa Chabielska, Anna Gromotowicz-Poplawska, Janusz Szemraj
Summary: This study found that the application of immobilized superoxide dismutase and catalase on nanoparticles can accelerate DNA repair and reduce harmful effects caused by UV radiation. The expression levels of nucleotide excision repair genes are increased with the application of immobilized enzymes.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2022)
Review
Psychiatry
Maria Dobielska, Natalia Karina Bartosik, Kamil A. Zyzik, Edward Kowalczyk, Michal Seweryn Karbownik
Summary: Depression not only leads to lowered mood but also cognitive impairment, affecting social functioning. However, cognitive function in depression has been neglected in the field of clinical and molecular psychiatry. This study discusses the mechanisms of cognitive decline in depression and proposes gut microbiota-targeted strategies as potential adjuvant therapeutics, providing a consolidated source of knowledge and paving the way for improved treatment options.
FRONTIERS IN PSYCHIATRY
(2022)
Article
Allergy
Michal Gabriel Panek, Michal Seweryn Karbownik, Karol Maksymilian Gorski, Marcelina Kocwin, Grzegorz Kardas, Mateusz Marynowski, Piotr Kuna
Summary: This study evaluates the impact of nasal allergen challenge and methacholine challenge test on mRNA expression changes in genes encoding the TGF-beta-Smad signaling pathway in asthmatic patients.
CLINICAL AND TRANSLATIONAL ALLERGY
(2022)
Article
Biochemistry & Molecular Biology
Michal Seweryn Karbownik, Paulina Sokolowska, Edward Kowalczyk
Summary: Butyrate and indole-3-propionic acid, gut microbiota metabolites, have potentially beneficial effects on human brain function and are being tested as antidepressants. In a human astrocyte cell-line culture, a 10-minute incubation with 1-5 mM butyrate significantly increased ATP gliotransmitter release by 78%, mediated by cytosolic Ca2+ mobilization. Indole-3-propionic acid had no significant effect on gliotransmitter release. Further evaluation is needed to confirm these findings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Microbiology
Layla Bleibel, Szymon Dziomba, Krzysztof Franciszek Waleron, Edward Kowalczyk, Michal Seweryn Karbownik
Summary: The intake of psychobiotic bacteria shows promise as a supplement to neuropsychiatric treatment, and may have positive effects on mental functioning in healthy individuals. Recent studies suggest that bacterial extracellular vesicles mediate the effects of psychobiotic bacteria on the brain, enhancing neurotrophic molecule expression, improving neurotransmission, and promoting neuroprotective mechanisms. These extracellular vesicles may have therapeutic applications as postbiotics. Further scientific exploration is needed to understand the complex nature of brain signaling mediated by bacterial extracellular vesicles. In conclusion, bacterial extracellular vesicles play a crucial role in the mechanism of action of psychobiotics.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Neurosciences
Lidia Wlodarczyk, Natalia Cichon, Michal Seweryn Karbownik, Luciano Saso, Joanna Saluk, Elzbieta Miller
Summary: This study aims to identify biomarkers that can predict brain repair ability and estimate the outcome of stroke by examining the expression of VEGF, IGF-1, and MMP-9 proteins and their genes. The results showed significant changes in clinical parameter estimations, as well as MMP9 and VEGF protein and mRNA expression over the rehabilitation process. The combined expression of MMP9 protein and mRNA may serve as a useful biomarker for cognitive improvement in post-stroke patients.
