Article
Biochemistry & Molecular Biology
Peng Ye, Xiaoxia Chi, Xiuwen Yan, Fangqin Wu, Zhigang Liang, Wen-Hao Yang
Summary: This study reveals the crucial role of alanine-glyoxylate aminotransferase (AGXT) in supporting liver cancer stem cells (LCSCs) and maintaining their stemness. AGXT may sustain the self-renewal potential of LCSCs by upregulating the expression of SRY-box transcription factor 2 (SOX2) and octamer-binding transcription factor 4 (OCT4).
Article
Oncology
Mikella Robinson, Samuel F. Gilbert, Jennifer A. Waters, Omar Lujano-Olazaba, Jacqueline Lara, Logan J. Alexander, Samuel E. Green, Gregory A. Burkeen, Omid Patrus, Zinia Sarwar, Ryne Holmberg, Christine Wang, Carrie D. House
Summary: The findings suggest that SOX2 may be a more consistent indicator of ovarian tumor initiating cells (TICs) that contribute to tumor repopulation following chemotherapy. Further studies evaluating SOX2 in TIC biology will enhance understanding of mechanisms driving ovarian cancer relapse.
Article
Oncology
Yunxia Li, Xin Chen, Wei He, Shuyue Xia, Xiaochuan Jiang, Xiaoyang Li, Jiayu Bai, Nan Li, Lei Chen, Biao Yang
Summary: The study suggests that apigenin (API) can suppress CD 133 positive cells and enhance the antitumor effect of CDDP in lung cancer cells, potentially eliminating cancer stem cells.
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Lucas Simoes Machado, Camila Martins Borges, Marina Amaro de Lima, Juliano Rodrigues Sangalli, Jacinthe Therrien, Lais Vicari de Figueiredo Pessoa, Paulo Fantinato Neto, Felipe Perecin, Lawrence Charles Smith, Flavio Vieira Meirelles, Fabiana Fernandes Bressan
Summary: Mechanisms of cell reprogramming by pluripotency-related transcription factors or nuclear transfer seem to be mediated by similar pathways. The study suggests that OCT4 and SOX2 may contribute to both processes and help elucidate the mechanisms responsible for pluripotency.
Article
Oncology
Wei Peng, Liang Chang, Wenqiang Li, Yanan Liu, Min Zhang
Summary: This study investigated the efficacy of OCT4&SOX2 specific cytotoxic T lymphocytes (CTLs) in combination with PD-1 inhibitor in treating breast cancer stem-like cells (BCSCs) and drug-resistance breast cancer (DRBC) mice. The results showed that OCT4 and SOX2 were associated with poor prognosis in breast cancer patients. In vitro experiments demonstrated that OCT4&SOX2 CTLs had enhanced cytotoxic activity against BCSCs, which was further improved by the addition of PD-1 inhibitor. In vivo experiments showed that the combination of OCT4&SOX2 CTLs and PD-1 inhibitor reduced tumor volume and weight, and increased tumor apoptosis rate.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Pan Wang, Lu Zhao, Sheng Gong, Shuanglong Xiong, Junwei Wang, Dewei Zou, Jinyu Pan, Yangmin Deng, Qian Yan, Nan Wu, Bin Liao
Summary: HIF1α and HIF2α regulate each other with a negative feedback loop, affecting GBM malignant progression through the EGFR-PI3K/AKT pathway and increasing sensitivity to chemotherapy. Their interaction with Sox2 and Klf4 further contributes to stemness expression and cell cycle arrest, enhancing malignancy in GBM.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Shimiao Huang, Xuan Wang, Yin Zhu, Yadong Wang, Jiaxuan Chen, Haoxuan Zheng
Summary: This study elucidates the mechanism by which SOX2 promotes VM formation in colorectal cancer. SOX2 overexpression enhances glycolysis and sustains VM formation through the transcriptional activation of lncRNA AC005392.2. Overexpression of AC005392.2 increases the stability of GLUT1 protein, leading to enhanced glycolysis and VM mediated by SOX2. Clinical analysis reveals a positive correlation between the expression levels of AC005392.2, GLUT1, and EPHA2 with SOX2, as well as poor prognoses in patients with CRC.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Paulina Gil-Kulik, Michal Lesniewski, Karolina Bienko, Monika Wojcik, Marta Wieckowska, Dominika Przywara, Alicja Petniak, Adrianna Kondracka, Malgorzata Swistowska, Rafal Szymanowski, Agnieszka Wilinska, Mateusz Wilinski, Bartosz J. Plachno, Marzena Kostuch, Mansur Rahnama-Hezavach, Mariusz Szuta, Anna Kwasniewska, Anna Bogucka-Kocka, Janusz Kocki
Summary: The expression of eight genes from the IAP family and the gene regulating IAP-XAF1 in stem cells derived from human milk was evaluated. The study revealed the relationships between gene expression and clinical data such as maternal age, maternal BMI, week of pregnancy, bodyweight of the newborn, number of pregnancies and deliveries, and time elapsed since delivery. This research is the first to investigate the expression of genes from the IAPs family in mother's milk stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Qian Yan, Xiaona Fang, Chenxi Li, Ping Lan, Xinyuan Guan
Summary: This review summarizes the common pluripotent transcription factors and development signaling pathways shared by cancer stem cells (CSCs) and embryonic stem cells (ESCs). It also discusses the newly identified oncofetal proteins that drive the self-renewal and therapy resistance of CSCs. Additionally, the review explores how targeting oncofetal drivers through clinical implementation can facilitate the development of CSCs-directed therapy.
ESSAYS IN BIOCHEMISTRY
(2022)
Article
Genetics & Heredity
Shuanglong Xiong, Donglin Wang, Yin Tang, Songmei Lu, Lumi Huang, Zhijuan Wu, Shuangyi Lei, Guanzhong Liang, Dan Yang, Dairong Li, Yan Li
Summary: Objective: To investigate the role of HIF1 alpha and HIF2 alpha in regulating the dedifferentiation of lung cancer cells under hypoxic conditions through Sox2 and Oct4. Methods: The expression of HIF1 alpha, HIF2 alpha, Sox2, and Oct4 was analyzed in lung cancer tissues. Sphere formation and the expression of CD133, CD44, Sox2, Oct4, HIF1 alpha, and HIF2 alpha were examined in differentiated lung cancer cells cultured under hypoxia. Knockout experiments were performed for HIF1 alpha, HIF2 alpha, Sox2, and Oct4, and the effects on CD133 and CD44 expression were evaluated. Results: Differentiated lung cancer cells formed more spheres under hypoxic conditions and exhibited higher expression of CD133 and CD44. TCGA database analysis revealed elevated levels of HIF1 alpha and HIF2 alpha in lung cancer tissues. Knockout of HIF1 alpha and HIF2 alpha resulted in decreased expression of Sox2, Oct4, CD133, and CD44, while knockout of Sox2 or Oct4 led to decreased expression of CD133 and CD44. Conclusion: HIF1 alpha and HIF2 alpha play a crucial role in regulating the dedifferentiation of non-small-cell lung cancer cells through Sox2 and Oct4 under hypoxic conditions.
Article
Cell Biology
Jun Zhang, Xiang Lv, Bo Wei, Xue Gong, Liming Chen
Summary: The research found that CHD4 can suppress the stemness of breast cancer by repressing the expression of SOX2, and this effect is dependent on TRPS1. These findings are important for understanding the regulation mechanism of breast cancer stem cells.
CELLULAR SIGNALLING
(2022)
Review
Pathology
Esmat Abdi, Saeid Latifi-Navid, Hamid Latifi-Navid
Summary: Breast cancer is the most prevalent cancer in females worldwide, with a complex interaction of variables like lifestyle, climate, genetics, and reproductive factors. Long non-coding RNAs (lncRNAs) play important roles in breast cancer development, with their polymorphisms predictive of cancer incidence and useful for early detection and customized therapy. Further large-scale trials and functional assessments are needed to validate the results and shed light on the etiology of breast cancer.
PATHOLOGY RESEARCH AND PRACTICE
(2022)
Article
Medicine, Research & Experimental
Zhihui Gao, Qianqing Wang, Mei Ji, Xiangcui Guo, Li Li, Xiaoke Su
Summary: The study revealed that UCA1 regulates SOX2 expression by sponging miR-122-5p, affecting the functions of CC stem cells, while CaSki-Exo promotes the invasion and migration of CC stem cells. Silencing UCA1 or up-regulating miR-122-5p reduced the invasion and migration abilities of CC stem cells.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Xupeng Bai, Jie Ni, Julia Beretov, Shanping Wang, Xingli Dong, Peter Graham, Yong Li
Summary: The study shows that THOC2 and THOC5 are upregulated in radioresistant TNBC cells, promoting stem-like properties and radioresistance. Silencing THOC2 or THOC5 expression decreases protein expression of SOX2 and NANOG, depletes stem-like properties, and causes radiosensitization in TNBC cells. Depletion of THOC2 or THOC5 blocks xenograft tumorigenesis and growth of radioresistant TNBC in vivo, suggesting potential therapeutic strategies against relapsed TNBC.
Article
Oncology
Yanping Gao, Nannan Zhang, Zihang Zeng, Qiuji Wu, Xueping Jiang, Shuying Li, Wenjie Sun, Jianguo Zhang, Yangyi Li, Jiali Li, Fajian He, Zhengrong Huang, Jinfang Zhang, Yan Gong, Conghua Xie
Summary: PCAT1 inhibits immune cell infiltration and promotes tumorigenesis and immunosuppression in NSCLC. Knockdown of PCAT1 restrains proliferation, increases apoptosis and inhibits cell metastasis. PCAT1 activates SOX2, which further accelerates tumorigenesis and immune suppression.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Mostafa Keshavarz, Malek Hossein Asadi, Ali Riahi-Madvar
JOURNAL OF CELLULAR BIOCHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Forough Hakiminia, Firooz Jannat Alipoor, Mostafa Keshavarz, Malek Hossein Asadi
Summary: Long non-coding RNA PNKY is significantly upregulated in breast tumors and plays a crucial role in the proliferation, migration, and epithelial-mesenchymal transition (EMT) of breast cancer cells. Knock down of PNKY restricts cell proliferation by promoting apoptosis, senescence, and cell cycle disruption, and PNKY may trigger EMT by upregulating miR-150 and restricting the expression of Zeb1 and Snail. This study provides new evidence on the expression and biological function of PNKY in cancer cells, highlighting its potential contribution to tumor growth and metastasis.
