Journal
FASEB JOURNAL
Volume 33, Issue 4, Pages 5510-5519Publisher
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.201801950R
Keywords
sirtuins; nutrition; lipid metabolism; NAD
Categories
Funding
- Danish Ministry of Food, Agriculture, and Fisheries
- Danish Medical Research Council
- Lundbeck Research Foundation
- Novo-Nordisk Research Foundation
- European Union [LSHM-CT-2004-005272]
- Danish Ministry of Science, Technology, and Innovation
- Danish Diabetes Academy [NNF17SA0031406]
- Alfred Benzon Foundation
- Council for Independent Research/Medicine [4092-00309]
Ask authors/readers for more resources
The nicotinamide adenine dinucleotide-dependent deacetylase, sirtuin (SIRT)1, in skeletal muscle is reduced in insulin-resistant states. However, whether this is an initial mechanism responsible for mediating insulin resistance in human skeletal muscle remains to be investigated. Also, SIRT1 acts as a mitochondrial gene transcriptional regulator and is induced by a short-term, high-fat diet (HFD) in human skeletal muscle. Whether saturated or unsaturated fatty acids (FAs) in the diet are important for this is unknown. We subjected 17 healthy, young men to a eucaloric control (Con) diet and 1 of 2 hypercaloric [+75% energy (E%)] HFDs for 3 d enriched in either saturated (Sat) FA (79 E% fat; Sat) or unsaturated FA (78 E% fat; Unsat). After Sat, SIRT1 protein content and activity in skeletal muscle increased (P < 0.05; similar to 40%) while remaining unchanged after Unsat. Whole-body insulin sensitivity and insulin-stimulated leg glucose uptake were reduced (P < 0.01; similar to 20%) to a similar extent compared to Con after both HFDs. We demonstrate a novel FA type-dependent regulation of SIRT1 protein in human skeletal muscle. Moreover, regulation of SIRT1 does not seem to be an initiating factor responsible for mediating insulin resistance in human skeletal muscle.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available