Article
Food Science & Technology
Dilinu Kuerban, Jing Lu, Zekun Huangfu, Liang Wang, Yanan Qin, Minwei Zhang
Summary: To enrich the flavor and preserve the polyphenolic content in grape juice during long-term storage, different strains of lactobacilli were screened and the optimal fermentation conditions were determined. The retention rate of total polyphenolic compounds remained at 50% after 45 days of storage at 4°C. Various metabolites, including polyphenolics, saccharides, and organic acids, were identified. The grape juice also contained volatile organic compounds (VOCs), mainly esters, aldehydes, and alcohols, which could be formed through complex metabolic pathways involving carboxylic acids and derivatives.
Article
Food Science & Technology
Yanan Qin, Haotian Xu, Ya Chen, Jing Lei, Jingshuai Sun, Yan Zhao, Weijia Lian, Minwei Zhang
Summary: To address the issue of fruit perishability, mulberry wine has been developed as a preservation method. This study used UHPLC-QE-MS/MS and multivariate statistical analyses to examine the metabolic profiles, specifically flavonoid profiles, during the process of vinification. The results identified differential metabolites and highlighted the role of total sugar and alcohol in driving the composition of various metabolites. Importantly, specific flavonoids were identified as differential metabolic markers during black mulberry wine fermentation and ripening. The study also revealed the major metabolic pathways of flavonoids in this process.
Article
Chemistry, Applied
Jian Ai, Qixia Wu, Maurizio Battino, Weibin Bai, Lingmin Tian
Summary: This study utilized UHPLC-QE-MS approach with multivariate statistical analyses to investigate the metabolic profiles, particularly anthocyanin profiles, during roselle wine fermentation. A variety of metabolites including anthocyanins were identified, with several metabolic pathways related to flavonoid and anthocyanin biosynthesis identified during the fermentation process. These findings will be beneficial for future research in wine chemistry and yeast metabolism.
Article
Biotechnology & Applied Microbiology
Xiantao Yan, Min Liu, Congcong Guo, Xinyue Lian, Yun Shen, Yang Liu, Yi Qian, Longfei Zhang, Wenqiong Wang, Dawei Chen, Jianya Qian, Ruixia Gu
Summary: In this study, metabolites in Shenheling (SHL) fermented by Lactobacillus fermentum were analyzed using UHPLC-QE-MS. A total of 102 differential metabolites in nine categories were identified before and after fermentation, including alkaloids, organic acids, and flavonoids. After fermentation, the contents of several metabolites associated with antioxidation, anti-obesity, hypoglycemic, antibacterial, and improved immunity effects were increased. KEGG pathway analysis revealed important metabolic pathways affected by fermentation.
FERMENTATION-BASEL
(2023)
Article
Agriculture, Multidisciplinary
Yuandi Zhang, Yi Shen, Jiao Niu, Fang Ding, Ying Ren, Xiaoxue Chen, Bei-Zhong Han
Summary: This study explored the differences in physicochemical properties, microbiome, and metabolome among different high-temperature Daqu samples. Different bacterial genera were found to play key roles in amino acid metabolism pathways, leading to characteristic differences in appearance and quality of Daqu.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Biochemical Research Methods
Huan Li, Zhenhua Qian, Yanbiao Zhao, Hui Zheng
Summary: In this study, the phase I metabolites of two new synthetic cannabinoids, 4F-MDMB-BICA and 4F-MDMB-BINACA, were identified using pooled human liver microsome (pHLM) and zebrafish combined with ultra-high-performance liquid chromatography (UHPLC) Q Exactive Orbitrap MS. A total of 14 metabolites of 4F-MDMB-BINACA and 16 metabolites of 4F-MDMB-BICA were detected, with one metabolite of 4F-MDMB-BINACA being discovered for the first time. Three symbol metabolites for each synthetic cannabinoid were recommended based on the toxicokinetics results.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Spectroscopy
Bai Feng-yuan, Zhao Dong-mei, Cai Ren-jun, Song Su-fei, Liu Tao, Xu Qiu-ling
Summary: This study used UHPLC-QE-MS non-targeted metabolomics method to observe the changes of endogenous metabolites in serum of CCI rats, screen out the serum differential metabolites, and analyze the effect of chronic pain on differential metabolites. The results showed that multiple metabolites in the serum of CCI rats were significantly different from the normal control group, providing insights into the mechanism of chronic pain and drug targets.
SPECTROSCOPY AND SPECTRAL ANALYSIS
(2022)
Article
Agriculture, Multidisciplinary
Jiaxu Yao, Li Zhou, Yueqi Hu, Minjie Zhao, Yue Ma, Jikai Liu, Eric Marchioni
Summary: Untargeted lipidomics analysis combined with chemometrics was used to identify different mushroom varieties. The extraction efficiency of different solvents for mushroom lipids was compared, and methyl tert-butylether/methanol (2:1, v/v) was found to be the most efficient solvent. Comprehensive lipidomics analysis identified 37 characteristic metabolites that could distinguish between poisonous and edible mushrooms. This study provides a novel method for identifying poisonous mushrooms and a reference for food safety.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Yunfeng Tang, Lixin Sun, Yun Zhao, Jingchun Yao, Zhong Feng, Zhong Liu, Guimin Zhang, Chenghong Sun
Summary: This study investigated the protective mechanism of Icaritin (ICT) against cerebral ischemic reperfusion injury using a metabolomics approach. The results revealed that ICT could suppress oxidative stress by activating the Nrf2 signaling pathway and regulating the production of arachidonic acid, linoleic acid, taurine, GABA, and NAAG in brain tissue, thereby reducing cerebral infarction and improving neurological deficits in mice.
Article
Agriculture, Multidisciplinary
Fu Yang, Minjie Zhao, Li Zhou, Minghao Zhang, Jikai Liu, Eric Marchioni
Summary: This study characterized and quantified the total lipids of eight wild edible mushrooms, identifying 20 lipid classes and 173 molecular species. Variations in lipid molecules and concentrations among different mushroom species were found, with potential biomarkers such as lysophosphatidylethanolamine (16:1) and ceramide non-hydroxy fatty acid-dihydrosphingosine (d23:0-10:0) identified. The study provides a new perspective for distinguishing mushroom species based on lipidomics.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Nutrition & Dietetics
Ya Xu, Pu-Lin Liang, Xue-Lian Chen, Ming-Jiong Gong, Liang Zhang, Xiao-Hui Qiu, Jing Zhang, Zhi-Hai Huang, Wen Xu
Summary: This study comprehensively investigated the chemical changes in pu-erh tea after cofermented with Citrus peel using advanced technology, revealing significant chemical profile disparities between Ganpu tea and raw pu-erh tea. The cofermentation process with Citrus peel greatly impacted the content and types of compounds in the tea.
FRONTIERS IN NUTRITION
(2021)
Article
Medicine, Research & Experimental
Liang Li, Jieyan Li, Haijie Cao, Qiuhong Wang, Zhengzheng Zhou, Hu Zhao, Haixue Kuang
Summary: Heroin can damage the liver and disrupt its metabolic pathways. Glutathione, taurine, riboflavin, 4-pyridoxate, folic acid, and methionine are important metabolic biomarkers that may be key targets of heroin-induced liver damage. This study provides insights into the mechanisms of heroin-induced hepatotoxicity and potential biomarkers of liver damage.
Article
Food Science & Technology
Jinrui Zhu, Li Zhou, Minjie Zhao, Fang Wei, Haiyan Fu, Eric Marchioni
Summary: This study investigated the dynamic changes in lipid composition during the roasting process of coffee beans using lipidomic analysis. Multiple lipid classes and molecular species were identified and quantified, showing distinct changes during roasting.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Plant Sciences
Jogendra Singh, Vijayata Singh, Vikas Dutt, Nisha Walia, Gayatri Kumawat, Mohan Lal Jakhar, Devendra Kumar Yadava, Parbodh Chander Sharma
Summary: This study investigates the global metabolomic response of two Indian mustard genotypes under salt stress to uncover the role of differentially accumulated metabolites and relevant metabolic pathways in the salt tolerance mechanism.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Heqin Yan, Wei Zheng, Zhouchen Ye, Jing Yu, Yougen Wu
Summary: This study used UHPLC/Q-TOF-MS and MSA to analyze the differences in chemical compounds of C. vietnamensis seeds among four maturation stages. The results showed that the metabolites in the seeds undergo dynamic changes during the development stages, including benzene and substituted derivatives, 5'-deoxyribonucleosides, and 1,3-diarylpropanoids. KEGG analysis further revealed differentially expressed metabolites at different growth periods.
Article
Energy & Fuels
Muhammad Zeeshan Siddique, Abdul Waheed Badar, Shan Ali Jakhrani, Fahad Sarfraz Butt, M. Salman Siddiqui, Muhammad Yasin Khan, Khalid Mahmood
Summary: This study conducted analytical modeling and theoretical analysis of a solar cooker cum dryer (SCCD) to predict its thermal characteristics and evaluate the influence of design parameters. The analytical model was tested and validated with experimental data, showing the effectiveness of the SCCD unit in food drying.
ENERGY SOURCES PART A-RECOVERY UTILIZATION AND ENVIRONMENTAL EFFECTS
(2022)
Article
Agronomy
Mueen Alam Khan, Hafiz Syed M. Arslan Iqbal, Muhammad Waseem Akram
Summary: In this two-year study, 25 diverse wheat genotypes from various research institutes of Pakistan were evaluated for their drought tolerance using physiological indices. Positive associations between certain indices and grain yield were found, and potential drought tolerant genotypes were identified. Principal component analysis revealed significant variation in response to control and drought stress conditions.
Article
Thermodynamics
Muhammad Zeeshan Siddique, Abdul Waheed Badar, M. Salman Siddiqui, Fahad Sarfraz Butt, Muhammad Saleem, Khalid Mahmood, Imran Fazal
Summary: This study simulates and analyzes the energy consumption of heating and/or cooling auxiliary devices in solar sorption cooling systems and investigates the influence of series versus parallel arrangement of solar collectors. The results demonstrate that configuration-2 (C-2) achieves higher primary energy savings and solar fraction at lower collector areas.
Article
Plant Sciences
Hafiz Ghulam Muhu-Din Ahmed, Yawen Zeng, Humayun Raza, Dur Muhammad, Muhammad Iqbal, Muhammad Uzair, Mueen Alam Khan, Rashid Iqbal, Ayman EL Sabagh
Summary: Salinity is a major abiotic stress that affects wheat production. Selecting salt-tolerant genotypes based on leaf water content and relative water content can improve the performance of wheat under salinity stress. Principal component analysis also indicates substantial genetic variation in response to salinity levels.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Agriculture, Dairy & Animal Science
Muhammad Fakhar-e-Alam Kulyar, Wangyuan Yao, Quan Mo, Yanmei Ding, Yan Zhang, Jindong Gao, Kewei Li, Huachun Pan, Shah Nawaz, Muhammad Shahzad, Khalid Mehmood, Mudassar Iqbal, Muhammad Akhtar, Zeeshan Ahmad Bhutta, Muhammad Waqas, Jiakui Li, Desheng Qi
Summary: Tibial dyschondroplasia (TD) is a common metabolic disorder in broilers caused by thiram consumption. It impairs bony and cartilage processes, resulting in lameness and mortality in commercial poultry. Cellular pathways involving anti-apoptotic, pro-apoptotic, and executioner caspases regulate the chondrocyte changes. The inflammatory response mediated by inflammasomes and mitochondrial-mediated apoptosis are important pathways in TD.
Article
Agronomy
Mueen Alam Khan, Muhammad Waseem Akram, Muhammad Iqbal, Hafiz Ghulam Muhu-Din Ahmed, Abdul Rehman, Hafiz Syed M. Arslan Iqbal, Beena Alam
Summary: This study assessed the association of quantitative attributes of wheat genotypes under normal and drought stress conditions. The results showed significant differences in traits between the two conditions. The correlation analysis revealed the highest positive association between grain yield and flag leaf area under normal conditions, and between biological yield and grain yield under drought stress. Moreover, genetic relatedness among genotypes was identified through cluster analysis.
COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS
(2023)
Review
Biotechnology & Applied Microbiology
Xiaoqing Liu, Riaz Hussain, Khalid Mehmood, Zhaoxin Tang, Hui Zhang, Ying Li
Summary: This review provides a brief summary of mitochondrial-endoplasmic reticulum communication-mediated oxidative stress and autophagy. The mitochondria and endoplasmic reticulum, as important organelles in cells, are connected to each other and jointly regulate oxidative stress and autophagy. The review explores the mutual connection of mitochondria and endoplasmic reticulum in mediating oxidative stress and autophagy through ROS and Ca2+ and aims to provide part of the theoretical basis for alleviating oxidative stress through autophagy mediated by mitochondrial-endoplasmic reticulum communication.
BIOMED RESEARCH INTERNATIONAL
(2022)
Article
Agronomy
Mueen Alam Khan, Muhammad Umar Ayyub, Ayesha Bashir, Beena Alam
Summary: Due to environmental fluctuations, wheat scientists need to develop new heat tolerant cultivars to increase and stabilize wheat production. A study was conducted to evaluate the genetic variability of twenty wheat genotypes under climate change scenarios. Significant differences were found among all genotypes for various morpho-phenological traits, and stronger correlations were observed under terminal heat stress, indicating the adversities of heat stress. Principal component analysis showed that the first three components explained a significant amount of cumulative variability in genotypes, and yield per plant had the highest variability. SUBHANI-21 exhibited the highest yield under heat stress, followed by LASANI-08 and UJALLA-16 for thousand grain weight. Selection of positively associated traits and utilization of tolerant genotypes with heat stress tolerance potential in future wheat breeding programs is important for developing high yielding and heat resilient genotypes.
Article
Infectious Diseases
Muhammad Adnan Saeed, Muhammad Saqlain, Usman Waheed, Syed Ehtisham-ul-Haque, Aman Ullah Khan, Aziz ur Rehman, Muhammad Sajid, Farhan Ahmad Atif, Heinrich Neubauer, Hosny El-Adawy
Summary: This study assessed the prevalence, antimicrobial resistance patterns, and risk factors associated with ESBL avian pathogenic E. coli isolated from backyard chickens in Pakistan. The results showed a high isolation rate of ESBL E. coli in Aseel chickens and a high level of resistance to multiple antibiotics. The study also identified the free-range husbandry management system and high antimicrobial usage as significant risk factors for the isolation of ESBL-producing E. coli.
Article
Parasitology
Fareeda Kebzai, Kamran Ashraf, Mujeeb Ur Rehman, Haroon Akbar, Muhammad Avais, Masam Khan
Summary: This study aimed to determine the prevalence and risk factors of theileriosis in sheep from Balochistan, Pakistan. The results showed that the prevalence of Theileria species was highest in district Zhob, with T. lestoquardi being the main species causing infection. Risk factors such as tick abundance and feeding pattern of animals were significantly correlated with the presence of T. lestoquardi.
PARASITOLOGY RESEARCH
(2023)
Article
Parasitology
Muhammad Sajid Ramzan, Muhammad Suleman, Muhammad Imran Rashid, Haroon Akbar, Muhammad Avais
Summary: This study evaluated the cell-mediated immune responses in calves administered with two types of T. annulata vaccine (live attenuated and killed). The results showed a significant increase in CD4+ and CD8+ T cells in immunized calves. There was a significant difference in the CD8+ T cell response between the post-challenge stage of killed and live vaccine, while no other difference was found at both pre- and post-immunization stages.
PARASITOLOGY RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Wen Zou, Sijia Lu, Jia Wang, Yixiao Xu, Muhammad Akbar Shahid, Muhammad Usman Saleem, Khalid Mehmood, Kun Li
Summary: This study aimed to evaluate the effects of microplastic (MP) exposure on the growth performance and gut microbiota of chickens. The results showed that MP exposure significantly affected the growth performance of chickens and led to an imbalance in the gut microbiota. This study provides evidence of the health threat caused by MP contamination and its relevance to environmental quality and poultry health.
Article
Veterinary Sciences
Shoaib Ahmad, Farhan Ahmad Atif, Arfan Zaman, Syed Nazar Abbas, Yasir Razzaq Khan, Ameer Hamza Rabbani, Muhammad Shahid, Omar Naseer, Ahmad Ali, Kashif Hussain
Summary: This study aimed to determine the molecular epidemiology of ehrlichiosis in dogs using blood smear microscopy and Polymerase chain reaction (PCR). The results showed that previous tick infestation history and housing hygiene were significant risk factors associated with the molecular prevalence of canine ehrlichiosis. The treatment trial concluded that minocycline along with Imidocarb dipropionate produced the highest recovery rates.
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
(2022)
Article
Veterinary Sciences
Muhammad Arfan Zaman, Azhar Rafique, Uzma Mehreen, Saba Mehnaz, Farhan Ahmad Atif, Asghar Abbas, Kashif Hussain, Muhammad Asif Raza, Sidra Altaf, Faisal Siddique, Rahman Md. Masudur, Mosaab Omar
Summary: A LAMP assay was developed for the diagnosis of T. lestoquardi, a major cause of ovine theileriosis. The assay showed high specificity and sensitivity and can be used as a diagnostic tool for the disease.
PAKISTAN VETERINARY JOURNAL
(2022)
Article
Veterinary Sciences
Muhammad Awais Zahoor, Shafia Tehseen Gul, Aisha Khatoon, Muhammad Ashraf, Muhammad Zubair, Muhammad Imran, Bakhtawar Maqbool, Farhan Ahmad Atif
Summary: The study investigated the teratogenic effects of thiamethoxam on the development of chick embryos. The results showed growth retardation, deformities, and significant effects on vital organs in the treated embryos.
PAKISTAN VETERINARY JOURNAL
(2022)