Journal
ENVIRONMENTAL MICROBIOLOGY
Volume 21, Issue 8, Pages 2836-2851Publisher
WILEY
DOI: 10.1111/1462-2920.14635
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Funding
- Sichuan Veterinary Medicine and Drug Innovation Group of China Agricultural Research System [CARS-SVDIP] Funding Source: Medline
- National Key Research and Development Program of China [2017YFD0500800] Funding Source: Medline
- China Agricultural Research System [CARS-42-17] Funding Source: Medline
- Special Fund for Key Laboratory of Animal Disease and Human Health of Sichuan Province [2016JPT0004] Funding Source: Medline
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Riemerella anatipestifer (RA) is a gram-negative bacterium that has a high potential to infect waterfowl. Although more and more genomes of RA have been generated comparaed to genomic analysis of RA still remains at the level of individual species. In this study, we analysed the pan-genome of 27 RA virulent isolates to reveal the intraspecies genomic diversity from various aspects. The multi-locus sequence typing (MLST) analysis suggests that the geographic origin of R. anatipestifer is Guangdong province, China. Results of pan-genome analysis revealed an open pan-genome for all 27 species with the sizes of 2967 genes. We identified 387 genes among 555 unique genes originated by horizontal gene transfer. Further studies showed 204 strain-specific HGT genes were predicted as virulent proteins. Screening the 1113 core genes in RA through subtractive genomic approach, 70 putative vaccine targets out of 125 non-cytoplasmic proteins have been predicted. Further analysis of these non A. platyrhynchos homologous proteins predicted that 56 essential proteins as drug target with more interaction partners were involved in unique metabolic pathways of RA. In conclusion, the present study indicated the essence and the diversity of RA and also provides useful information for identification of vaccine and drugs candidates in future.
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