Review
Pharmacology & Pharmacy
Mohammad Samiei, Marziyeh Fathi, Jaleh Barar, Nazanin Fathi, Nazanin Amiryaghoubi, Yadollah Omidi
Summary: Regenerative medicine is a promising and rapidly growing field that aims to develop new functional tissues to treat defective cells/tissues and achieve precision medicine. To achieve optimal clinical outcomes, efficient and safe delivery of cell-based medicines to damaged areas is essential.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Medicinal
Julia Ohmes, Lena Marie Saure, Fabian Schuett, Marie Trenkel, Andreas Seekamp, Regina Scherliess, Rainer Adelung, Sabine Fuchs
Summary: In this study, an injectable thermosensitive hydrogel was developed for the delivery of fucoidan and MSC for use in regenerative medicine. The hydrogel exhibited good physicochemical properties and biocompatibility, and was able to release fucoidan over a certain period of time.
Article
Engineering, Biomedical
Ross E. B. Fitzsimmons, Ronald G. Ireland, Aileen Zhong, Agnes Soos, Craig A. Simmons
Summary: One approach to engineering viable tissues ex vivo involves using hydrogel-based microfluidics to generate microvessels, with a focus on collagen, fibrin, and collagen-fibrin co-gel formulations. Fibrin and co-gels were found to promote multicellular EC sprouting, while collagen required supplementation to elicit a migration response. Increasing collagen content in co-gel formulations led to higher compressive modulus and reliable formation of intact hydrogel-based microchannels for perfusion, making collagen-fibrin co-gels a promising option for generating vascularized tissue constructs.
BIOMEDICAL MATERIALS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Christina Hadjichristou, Eleni Papachristou, Emmanouil Vereroudakis, Maria Chatzinikolaidou, Imad About, Petros Koidis, Athina Bakopoulou
Summary: A three-dimensional dentin/pulp tissue analogue has been engineered in vitro for evaluating the cytocompatibility of resin-based dental restorative cements. The results showed that both cements exhibited good cytocompatibility and triggered angiogenic responses within the dentin/pulp tissue analogue.
Review
Chemistry, Medicinal
Teerawat Sukpaita, Suwabun Chirachanchai, Atiphan Pimkhaokham, Ruchanee Salingcarnboriboon Ampornaramveth
Summary: This review provides an overview of chitosan-based biomaterials for bone tissue engineering and their applications in treating bone defects. The focus is on the characteristics of chitosan, scaffold fabrication processes, advantages and disadvantages, and future directions in the field.
Article
Polymer Science
Muhammad Umar Aslam Khan, Zahida Yaqoob, Mohamed Nainar Mohamed Ansari, Saiful Izwan Abd Razak, Mohsin Ali Raza, Amna Sajjad, Sajjad Haider, Fauzi Mh Busra
Summary: Composite hydrogels of CS/PVA and GO/CS/PVA were successfully synthesized and analyzed using various techniques, showing good swelling and drug release properties. The study also revealed better antibacterial and cell viability behaviors of the composite materials, indicating their potential as biomaterials in biomedical applications.
Article
Pharmacology & Pharmacy
Mohammad Samiei, Elaheh Dalir Abdolahinia, Marziyeh Fathi, Jaleh Barar, Yadollah Omidi
Summary: In this study, chitosan-based hydrogels were used to improve the proliferation and differentiation of dental pulp stem cells (DPSCs), with CS/GN hydrogel showing better features in enhancing DPSCs viability and osteogenic differentiation.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Thibault Canceill, Geraldine Jourdan, Philippe Kemoun, Christophe Guissard, Yanad Abou Monsef, Marion Bourdens, Benoit Chaput, Sandrine Cavalie, Louis Casteilla, Valerie Planat-Benard, Paul Monsarrat, Isabelle Raymond-Letron
Summary: Adipose-derived mesenchymal stromal cells (ASC) transplantation is a promising strategy for tissue regeneration. This study aimed to optimize the generation phase of a platelet-lysate-based fibrin hydrogel (PLFH) as a proper carrier for in situ ASC implantation, and comprehensively assess its in vitro properties and in vivo safety profile.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Nuoya Zhao, Weizhong Yuan
Summary: A multi-dynamically crosslinked nanocomposite hydrogel was prepared in this study using dextran modified with aldehyde groups and phenylboronic acid esters, carboxymethyl chitosan, polyaniline grafted chitosan oligosaccharide, and Epigallocatechin Gallate/Ca2+ modified melanin-like nanoparticles. The hydrogel showed good photothermal conversion properties, antioxidant activity, and mechanical properties. It could also record respiratory and heart rate signals in real time and distinguish them accurately using a convolutional neural network algorithm. This multifunctional nanocomposite hydrogel provides a new strategy for promoting chronic wound healing and effective health monitoring.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Fatemeh Mirjalili, Mahboobeh Mahmoodi
Summary: In this study, platelet-rich fibrin-chitosan (CH-PRF) nanoparticles were loaded into gelatin-chitosan hydrogel (Gel-CH/CH-PRF) to create a hydrogel dressing for wound healing. The Gel-CH/CH-PRF hydrogel exhibited excellent swelling behavior, good porosity, high absorption of wound exudates, and proper vapor permeability rate. It also demonstrated sustained release of proteins and strong antimicrobial activity. In vivo experiments showed that the Gel-CH/CH-PRF hydrogel promoted wound closure, increased epidermis thickness, and enhanced the formation of new granulation tissue. These findings highlight the potential of Gel-CH/CH-PRF hydrogel as an effective wound dressing.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Veterinary Sciences
Maria Soltero-Rivera, Sterling Hart, Andrew Blandino, Natalia Vapniarsky, Boaz Arzi
Summary: This study retrospectively evaluated the long-term safety and efficacy of MSC therapy for refractory feline chronic gingivostomatitis (FCGS) and investigated potential factors associated with treatment outcomes. The overall positive response rate to MSC treatment was 65.5%, with 58.6% of cats exhibiting permanent improvement or cure. Adverse effects occurring during or immediately after treatment were noted in 34.2% of cases, the majority being transient, self-resolving transfusion-like reactions. No long-term adverse events were noted.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Biotechnology & Applied Microbiology
Yuhang Tian, Danhua Wu, Dankai Wu, Yutao Cui, Guangkai Ren, Yanbing Wang, Jincheng Wang, Chuangang Peng
Summary: This review discusses the role and application of chitosan in the treatment of infected bone defects. Chitosan, as an antibacterial natural polymer, has been widely studied in bone tissue engineering. By promoting the adhesion and proliferation of osteoblast-related cells, chitosan can provide an ideal carrier for bone repair. In addition, the properties of functionalized chitosan are analyzed and compared. The latest research on the combination of chitosan with other materials and biomolecules for the treatment of infected bone defects is summarized in this review.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Maria Luisa Leite, Diana Gabriela Soares, Giovana Anovazzi, Caroline Anselmi, Josimeri Hebling, Carlos Alberto de Souza Costa
Summary: By varying the ratio of collagen and gelatin and incorporating different concentrations of fibronectin, the study found that collagen/gelatin hydrogel with 10 mg/mL of FN exhibited potent bioactive and chemotactic effects on human apical papilla cells.
JOURNAL OF ENDODONTICS
(2021)
Article
Biochemistry & Molecular Biology
Hengji Zhao, Yushu Zhang, Chao Zhou, Chunling Zhang, Bo Liu
Summary: Natural polysaccharide-based hydrogels provide effective skin protection during trauma and exhibit good antibacterial properties.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Materials Science, Paper & Wood
Bo-Nan Jiang, Dan Li, Xue-Na Zou, Hui-Ge Wei, Cheng Zhong, Li-Qiang Chu
Summary: Biopolymer-based injectable hydrogels with antibacterial activity have great potential for biomedical applications. In this study, a double network hydrogel was successfully prepared, which exhibited excellent injectability and antibacterial activity.
Review
Chemistry, Applied
S. Peers, A. Montembault, C. Ladaviere
Summary: This review focuses on the development of complex drug delivery systems composed of chitosan hydrogels embedding different types of colloids to achieve delayed drug release. The review also provides detailed information on the biomedical applications of these final systems.
CARBOHYDRATE POLYMERS
(2022)
Article
Engineering, Biomedical
Ysander von Boxberg, Sylvia Soares, Camille Giraudon, Laurent David, Maud Viallon, Alexandra Montembault, Fatiha Nothias
Summary: Direct contact with chitosan-FPHS can modify the polarization of bone marrow-derived macrophages, favoring the generation of anti-inflammatory subtypes. Lower degree of acetylation and higher chitosan concentration promote macrophage polarization towards anti-inflammatory subtypes.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Infectious Diseases
Andrea Cara, Tristan Ferry, Frederic Laurent, Jerome Josse
Summary: Gram-negative bacilli can cause prosthetic joint infection, and the treatment becomes challenging due to increased antibiotic resistance and biofilm formation. Loading bone cement with antibiotics can help reduce the risk of infection during arthroplasties with cemented prosthesis. The study compared the efficacy of various antibiotic-loaded bone cements in preventing biofilm formation by different strains of Gram-negative bacteria.
Article
Biochemistry & Molecular Biology
Flor Estefany Bentley, Renaud Passieux, Laurent David, Anayancy Osorio-Madrazo
Summary: This study investigated the effects of low molar mass chitosan on chitosan fibers with bimodal distributions of molar masses. The results showed that adding low molar mass chitosan slightly increased the crystallinity of chitosan films, but decreased the crystallinity and altered the mechanical properties of stretched fibers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Materials Science, Biomaterials
Renaud Passieux, Guillaume Sudre, Alexandra Montembault, Martine Renard, Agnes Hagege, Pierre Alcouffe, Ali Haddane, Marie Vandesteene, Nadege Boucard, Laurence Bordenave, Laurent David
Summary: This study investigates the wet spinning of cytocompatible, bioresorbable, and knittable chitosan monofilaments with incorporated Cu2+ and Zn2+ ions. The results show that a concentration of Cu2+ at 0.01 and Zn2+ at 0.03 significantly enhances the antibacterial activity without compromising cytocompatibility. Additionally, the wet-spun CTS-Cu monofilaments are found to be knittable under industrial conditions.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Pharmacology & Pharmacy
Paul Rocchi, Delphine Brichart-Vernos, Francois Lux, Isabelle Morfin, Laurent David, Claire Rodriguez-Lafrasse, Olivier Tillement
Summary: This study developed a multimodal ultrasmall nanoparticle, CuPRiX, for cancer treatment. CuPRiX nanoparticles can prevent tumor cell dissemination and metastasis by depleting copper in tumors, and they also increase the sensitivity of tumors to radiotherapy.
Article
Microbiology
Aubin Souche, Camille Kolenda, Jordan Teoli, Raymond Schuch, Tristan Ferry, Frederic Laurent, Jerome Josse
Summary: This study evaluated the activity of the lysin exebacase against Staphylococcus epidermidis biofilms. It found that exebacase significantly reduced the biomass and bacterial count of S. epidermidis biofilms, and exhibited synergistic effects with rifampicin, vancomycin, or daptomycin. These findings suggest that exebacase could be a promising therapy for prosthetic joint infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Polymer Science
Clementine Beutier, Anatoli Serghei, Philippe Cassagnau, Patrick Heuillet, Bernard Cantaloube, Nathan Selles, Isabelle Morfin, Guillaume Sudre, Laurent David
Summary: In this study, in situ coupled mechanical/electrical/WAXS/SAXS investigations were carried out on EPDM-based composite materials filled with carbon black (CB) under uniaxial tensile stretching. The study reveals the correlation between the electrical conductivity and the microstructural state of the composite, providing insights into the mechanisms governing the structure/properties relationships with increasing deformation. The formation of nanovoids at large deformations leads to a significant decrease in conductivity. This study contributes to a better understanding of the conductivity/strain relationships of carbon black filled elastomers and has potential applications in developing smart rubber materials.
Article
Chemistry, Physical
Arnaud Kamdem Tamo, Tuan Anh Tran, Ingo Doench, Shaghayegh Jahangir, Aastha Lall, Laurent David, Carlos Peniche-Covas, Andreas Walther, Anayancy Osorio-Madrazo
Summary: This study proposes a 3D printing method for a multifunctional hydrogel biomaterial with bioactivity, good mechanical properties, and biodegradability. The method utilizes encapsulated cellulase and cellulose nanofiber filled chitosan viscous suspensions as bioinks to 3D print enzymatic biodegradable and biocompatible cellulose nanofiber reinforced chitosan hydrogels. The incorporation of the enzyme within the hydrogel helps control the degradation of cellulose while maintaining the cell growth-promoting property of chitosan. The 3D printed composite hydrogels exhibit excellent cytocompatibility and biodegradability.
Review
Chemistry, Medicinal
Hussein H. Genedy, Thierry Delair, Alexandra Montembault
Summary: Vectorization of microRNAs using polymeric nanoparticles, particularly chitosan, has great potential for treating various diseases. Chitosan, being biodegradable and biocompatible, can easily encapsulate microRNA and form polyplexes. However, fine tuning of chitosan's characteristics is crucial for successful formulation. This review summarizes chitosan microRNA formulations studied in the past decade, highlighting differences in materials, formulation processes, and intended applications. Factors contributing to the superiority of optimized systems are also discussed to maximize the potential of chitosan microRNA nanocarriers.
Article
Multidisciplinary Sciences
Ines Rodrigues Lopes, Laura Maria Alcantara, Ricardo Jorge Silva, Jerome Josse, Elena Pedrero Vega, Ana Marina Cabrerizo, Melanie Bonhomme, Daniel Lopez, Frederic Laurent, Francois Vandenesch, Miguel Mano, Ana Eulalio
Summary: This study investigates the interaction of 191 clinical isolates of Staphylococcus aureus with host cells, revealing the prevalence of intracellular replication and persistence. The study highlights the impact of phenotypic variation on infection characteristics and suggests potential implications for the treatment of staphylococcal infections.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Marie Chavignon, Ludivine Coignet, Melanie Bonhomme, Marine Bergot, Anne Tristan, Paul Verhoeven, Jerome Josse, Frederic Laurent, Marine Butin
Summary: This study investigated the mechanisms involved in the persistence of the clone NRCS-A in neonatal intensive care units (NICUs). The ability to form biofilm under nutrient stress and resist desiccation were identified as two major advantages of NRCS-A. These findings are crucial for developing targeted methods to combat NRCS-A and prevent its dissemination.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Valentina Andretto, Giuseppe Taurino, Giulia Guerriero, Hanae Guerin, Emmanuelle G. Laine, Massimiliano Bianchi, Geraldine Agusti, Stephanie Briancon, Ovidio Bussolati, Alexandra Clayer-Montembault, Giovanna Lollo
Summary: Oral administration of nanoparticles (NPs) faces challenges in the gastrointestinal environment, hindering their efficacy. To tackle this issue, nanocomposites with bio- and mucoadhesive matrixes were developed to improve the retention time of NPs in the intestine. Tailored nanoemulsions (NEs) were produced using a microfluidic-based approach and successfully encapsulated small hydrophobic drugs. These NEs demonstrated effective internalization into human intestinal cells and targeted delivery of drugs, offering potential for intestinal disease treatment.
Review
Nanoscience & Nanotechnology
Christy Kwokdinata, Vaibavi Ramanujam, Jiahui Chen, Paula Nunes de Oliveira, Mui Hoon Nai, Wai Hon Chooi, Chwee Teck Lim, Shi Yan Ng, Laurent David, Sing Yian Chew
Summary: This study found that using injectable hyaluronan-gelatin hydrogel in transplantation therapy can promote cell survival and neural differentiation of human spinal cord progenitor cells.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Medicine, General & Internal
Elsa Labrune, Cyrielle Fournier, Benjamin Riche, Laurent David, Alexandra Montembault, Sophie Collardeau-Frachon, Mehdi Benchaib, Jacqueline Lornage, Jean Iwaz, Bruno Salle
Summary: This study tested the long-term development ability of human ovarian cortex cells in unconventional culture conditions and found that bioreactor cultures slightly outperformed traditional cultures in folliculogenesis. This has important implications for follicle preservation and long-term toxicological studies.
MEDICINA-LITHUANIA
(2022)