Journal
CRYOBIOLOGY
Volume 87, Issue -, Pages 91-98Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cryobiol.2019.01.012
Keywords
Vitrification; Mouse; MII oocyte; DNA methylation; Reprogramming
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Funding
- Anhui Provincial Natural Science Foundation [1708085QC55, 1708085MC81]
- Science and Technology Major Project of Anhui province [18030701185]
- Natural Science Project of Universities in Anhui Province [102017A131, KJ2016A227]
- Young Scholar Natural Science Foundation of Anhui Agricultural University [2016ZR020]
- High-level Talent Research Foundation of Anhui Agricultural University [YJ2016-10]
- Open Fund of Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding [AKLGRCB2017003]
- Open Foundation of State Key Laboratory of Agrobiotechnology [2018SKLAB6-3]
- Open Fund of State Key Laboratory of Genetic Resources and Evolution [GREKF18-16]
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Accurate reprogramming of DNA methylation occurring in preimplantation embryos is critical for normal development of both fetus and placenta. Environmental stresses imposed on oocytes usually cause the abnormal DNA methylation reprogramming of early embryos. However, whether oocyte vitrification alters the reprogramming of DNA methylation (5 mC) and its derivatives in mouse preimplantation embryo development remains largely unknown. Here, we found that the rate of cleavage and blastocyst formation of embryos produced by IVF of vitrified matured oocytes was significantly lower than that in control counterparts, but the quality of blastocysts was not impaired by oocyte vitrification. Additionally, although vitrification neither altered the dynamic changes of 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5 fC) before 4-cell stage nor affected the levels of 5 mC and 5-carboxylcytosine (5caC) throughout the preimplantation development, vitrification significantly reduced the levels of 5hmC and 5 fC from 8-cell stage onwards. Correspondingly, vitrification did not alter the expression patterns of Tet3 in preimplantation embryos but apparently reduced the expression levels of Tet1 in 4-cell and 8-cell embryos and increased the expression levels of Tet2 at morula stage. Taken together, these results demonstrate that oocyte vitrification perturbs DNA methylation reprogramming in mouse preimplantation embryo development.
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