Article
Biochemistry & Molecular Biology
Konstantinos Loukelis, Foteini Machla, Athina Bakopoulou, Maria Chatzinikolaidou
Summary: This study investigates the impact of kappa-carrageenan on dental pulp stem cells and reveals that its integration in scaffolds significantly enhances the odontogenic potential of DPSCs, supporting dentin-pulp regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jan Schmidt, Vojtech Pavlik, Jakub Suchanek, Kristina Nesporova, Tomas Soukup, Martin Kapitan, Nela Pilbauerova
Summary: Hyaluronic acid (HA), a widely present biopolymer in the human body, can be used as a scaffold material for cells. It has been found that different molecular weights of HA have negligible effects on the viability and differentiation potential of human dental pulp stem cells (hDPSCs). Therefore, HA is a suitable component for hDPSCs-containing scaffolds.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Georgia Vagropoulou, Maria Trentsiou, Anthie Georgopoulou, Eleni Papachristou, Oleg Prymak, Aristeidis Kritis, Matthias Epple, Maria Chatzinikolaidou, Athina Bakopoulou, Petros Koidis
Summary: This study demonstrates the potential of hybrid chitosan/gelatin/nanohydroxyapatite scaffolds to support viability and proliferation of dental pulp stem cells, inducing odontogenic differentiation and in vitro biomineralization without the addition of any inductive factors. The results suggest a promising strategy for tissue engineering of mineralized dental tissues.
Review
Pharmacology & Pharmacy
Mohammad Samiei, Marziyeh Fathi, Jaleh Barar, Nazanin Fathi, Nazanin Amiryaghoubi, Yadollah Omidi
Summary: Regenerative medicine is a promising and rapidly growing field that aims to develop new functional tissues to treat defective cells/tissues and achieve precision medicine. To achieve optimal clinical outcomes, efficient and safe delivery of cell-based medicines to damaged areas is essential.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jan Schmidt, Nela Pilbauerova, Tomas Soukup, Tereza Suchankova-Kleplova, Jakub Suchanek
Summary: Hyaluronic acid and dental pulp stem cells show great promise for tissue engineering, but the effects of different molecular weights of HA on stem cells vary. Low molecular weight HA initially leads to reduced proliferation in stem cells, but they recover in subsequent passages, along with a shift in surface marker expression.
Review
Biotechnology & Applied Microbiology
Qiao Ruan, Shenglong Tan, Li Guo, Dandan Ma, Jun Wen
Summary: Prevascularization techniques have been proposed to solve the challenge of rapid vascularization in pulp regeneration. These techniques involve coculturing endothelial cells and pericytes to induce intercellular communication, and then introducing them into a customized artificial vascular bed or inducing self-assembly. However, there are still unresolved problems regarding cell sources, intercellular communication, and the construction of prevascularization systems for pulp regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
David Muallah, Jan Matschke, Matthias Kappler, Lysann Michaela Kroschwald, Gunter Lauer, Alexander W. Eckert
Summary: Xerostomia, or dry mouth, is mainly caused by hypofunction of the salivary glands due to various factors. Current treatment methods are not very effective and regenerative medicine using dental pulp stem cells shows promise. Dental pulp stem cells can differentiate into different cell types and have immunomodulatory effects. However, more clinical studies are needed to fully understand their potential for salivary gland regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Zhuo Xie, Zongshan Shen, Peimeng Zhan, Jiayu Yang, Qiting Huang, Shuheng Huang, Lingling Chen, Zhengmei Lin
Summary: Treatment of pulpal and periapical diseases faces challenges, but significant progress has been made in achieving true pulp tissue regeneration through technologies such as stem cells, biomaterials, and growth factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Negar Mansouri, Said Al-Sarawi, Dusan Losic, Jagan Mazumdar, Jillian Clark, Stan Gronthos, Ryan O'Hare Doig
Summary: This study investigates the biological effects of 3D graphene oxide/sodium alginate and reduced graphene oxide scaffolds on dental pulp stem cells. Graphene enhances degradation rate, cell viability, and supports higher stem cells viability. Larger pore size and poly-l-lysine coating improve cell adhesion and metabolism, while GO-based scaffolds allow seeding in serum-free media without cytotoxic effects.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Anna Zawadzka-Knefel, Agnieszka Rusak, Monika Mrozowska, Tomasz Machalowski, Andrzej Zak, Katarzyna Haczkiewicz-Lesniak, Michal Kulus, Piotr Kuropka, Marzenna Podhorska-Okolow, Katarzyna Skoskiewicz-Malinowska
Summary: The use of stem cells for tissue regeneration is a prominent trend in regenerative medicine and tissue engineering. Dental pulp stem cells (DPSCs) have attracted attention due to their ability to differentiate into osteoblasts and odontoblasts. This study explored the biological responses of human DPSCs to a purified 3D chitin scaffold derived from the marine demosponge Aplysina fistularis. Results showed that the chitin scaffold, especially with hydroxyapatite (HAp) modification, supported cell adhesion, proliferation, and differentiation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Marius Heitzer, Ali Modabber, Xing Zhang, Philipp Winnand, Qun Zhao, Felix Marius Blaesius, Eva Miriam Buhl, Michael Wolf, Sabine Neuss, Frank Hoelzle, Frank Hildebrand, Johannes Greven
Summary: This study investigated the osteogenic capability of DPSCs on different bone grafts. The results showed that the DBBM scaffold significantly enhanced the proliferation and osteogenic differentiation of DPSCs, indicating its potential for bone regeneration treatment.
Article
Biochemistry & Molecular Biology
Manuel Mata, Santiago Peydro, Jose Javier Martin de Llano, Maria Sancho-Tello, Carmen Carda
Summary: Periodontitis is an inflammatory disease that can lead to tooth loss. This study examined the role of surface microtopography in the differentiation of human dental pulp stem cells into cementoid-like secreting cells. The results showed that the cells cultured on dentin surfaces exhibited an odontoblastic phenotype, while cells cultured on cementum surfaces generated a well-organized tissue consisting of secretory cells and dense fibrous connective tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Senem Buyuksungur, Vasif Hasirci, Nesrin Hasirci
Summary: The study fabricates 3D printed hybrid scaffolds using PCL and GelMA carrying DPSCs, combining osteoinductivity support from GelMA and mechanical strength from PCL for bone tissue engineering applications. The hybrid scaffolds showed high stability and compressive moduli similar to trabecular bone. DPSCs exhibited high viability and osteogenic differentiation within GelMA, indicating great potential for bone tissue engineering applications.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Materials Science, Ceramics
Jose Guilherme Neves, Daniel Navarro da Rocha, Camila Cristine Lopes, Rafael Maza Barbosa, Lorena Fernandes Ferreira, Cecilia Buzatto Westin, Angela Maria Moraes, Bruno Calsa, Milton Santamaria Jr, Lourenco Correr-Sobrinho, Americo Bortolazzo Correr
Summary: The aim of this study was to synthesize and characterize polymeric porous scaffolds associated with different calcium phosphates (CaP) and Mesenchymal Stem Cells (MSC) for regenerative dentistry application. The results showed that CaP improved the mechanical properties of scaffolds but decreased the cell viability. MSCs improved the inflammatory response after 60 days.
CERAMICS INTERNATIONAL
(2022)
Review
Polymer Science
Vinna K. K. Sugiaman, Jeffrey, Silvia Naliani, Natallia Pranata, Rudy Djuanda, Rosalina Intan Saputri
Summary: The challenge in dentistry is to revitalize dental pulp using tissue engineering technology, which requires a biomaterial scaffold. The scaffold acts as a 3D framework that supports cell growth and provides a suitable environment for cell activation and communication. Selecting a safe, biocompatible scaffold with adequate characteristics is crucial in regenerative endodontics. Natural or synthetic polymer scaffolds have gained attention due to their excellent mechanical properties and good biological characteristics for cell regeneration. This review discusses the latest developments in utilizing these scaffold polymers for dental pulp tissue regeneration.
Article
Dentistry, Oral Surgery & Medicine
Caroline Anselmi, Igor Paulino Mendes Soares, Maria Luisa Leite, Fernanda Ali Kitagawa, Carlos Alberto de Souza Costa, Josimeri Hebling
Summary: This study aimed to characterize polycaprolactone-based nanofiber scaffolds incorporated with calcium hydroxide (CH) and evaluate their bioactivity on human dental pulp cells (HDPCs) when loaded with fibronectin (FN). The results showed that the incorporation of CH enhanced the odontogenic potential of HDPCs. Additionally, scaffolds loaded with fibronectin increased cellular migration, spread, and viability. These findings have important implications for dentin tissue engineering.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Juliana Silva Ribeiro, Ester Alves Ferreira Bordini, Gabriel Kalil Rocha Pereira, Rohitha Rao Polasani, Cristiane Helena Squarize, Karla Zanini Kantorski, Luiz Felipe Valandro, Marco Cicero Bottino
Summary: Coating denture base material with cinnamon-laden nanofibers effectively reduces Candida albicans adhesion and proliferation, inhibiting fungal growth. Applying 20 wt.% cinnamon-laden nanofibers leads to a significant reduction in the growth of C. albicans while maintaining the viability of epithelial cells.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Biochemistry & Molecular Biology
Juliana S. Ribeiro, Eliseu A. Muenchow, Ester A. F. Bordini, Nathalie S. Rodrigues, Nileshkumar Dubey, Hajime Sasaki, John C. Fenno, Steven Schwendeman, Marco C. Bottino
Summary: This study engineered cytocompatible and injectable antibiotic-laden fibrous microparticles GelMA hydrogels for endodontic infection ablation. The modified GelMA with antibiotic-laden fibrous microparticles increased the hydrogel swelling ratio and degradation rate, while exhibiting low toxicity to cells and effective antibiofilm effects against bacteria. Overall, these engineered hydrogels show promising prospects for clinical treatment of endodontic infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Camila Correa da Silva Braga Melo, Fernanda Balestrero Cassiano, Erika Soares Bronze-Uhle, Vitor de Toledo Stuani, Ester Alves Ferreira Bordini, Marjorie de Oliveira Gallinari, Carlos Alberto Souza Costa, Diana Gabriela Soares
Summary: The study assessed the characteristics of chitosan-based scaffolds enriched with mineral phases and biomineralized in simulated body fluid (SBF) for dentin regeneration. The results showed that mineral-containing scaffolds had increased pore size and porosity degree, promoting cell proliferation and odontogenic differentiation. Biomineralization enhanced the capacity for mineralized matrix deposition by pulpal cells. These findings suggest that chitosan scaffolds with biomineralization have potential applications in dentin tissue engineering.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Marlon Ferreira Dias, Beatriz Voss Martins, Rafael Antonio de Oliveira Ribeiro, Maria Luisa Leite, Uxua Ortecho-Zuta, Josimeri Hebling, Carlos Alberto de Souza Costa
Summary: Coating the enamel with PCP before applying the bleaching gel can maintain the esthetic efficacy of bleaching and reduce cytotoxicity to the dental pulp.
JOURNAL OF ESTHETIC AND RESTORATIVE DENTISTRY
(2023)
Article
Oncology
Tatiane Miranda Manzoli, Joatan Lucas de Sousa Gomes Costa, Joao Felipe Besegato, Mariana Bena Gelio, Lucas David Galvani, Ester Alves Ferreira Bordini, Milton Carlos Kuga, Andrea Abi Rached Dantas
Summary: This study showed that the use of 35% hydrogen peroxide gel in combination with violet LED can improve bleaching efficacy without negatively impacting pH and temperature. Additionally, the renewal of hydrogen peroxide did not influence the outcomes under these experimental conditions.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Taisa Nogueira Pansani, Fernanda Goncalves Basso, Lais Medeiros Cardoso, Carlos Alberto de Souza Costa
Summary: This study evaluated the metabolism of oral mucosal cells cultured on titanium discs coated with or without epidermal growth factor (EGF) and exposed to tumor necrosis factor alpha (TNF-alpha). The results showed that EGF coating increased cell viability and modulated the response of oral cells to an inflammatory stimulus.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Dentistry, Oral Surgery & Medicine
Lais Medeiros Cardoso, Taisa Nogueira Pansani, Carlos Alberto de Souza Costa, Fernanda Goncalves Basso
Summary: This study aimed to assess the effects of pre-treatment with proanthocyanidins (PA) flavonoids, from grape seed extract, and synthetic naringenin (NA) on the synthesis of matrix metalloproteinases (MMPs) gelatinases and their tissue inhibitors (TIMPs), as well as the gelatinolytic activity of MMPs by human gingival fibroblasts (HGF) and osteoblasts (Ob) exposed to zoledronic acid (ZA) in a dental implant surface in vitro model. The results showed that pre-treatment with NA and PA effectively regulated the harmful effects of ZA, reducing the synthesis and activity of MMP-2 and MMP-9 by HGF and Ob, while up-regulating the synthesis of TIMP-2 by Ob.
ARCHIVES OF ORAL BIOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Isabela dos Reis Souza, Maria Luisa Leite, Rafael Antonio de Oliveira Ribeiro, Josimeri Hebling, Carlos Alberto de Souza Costa
Summary: The purpose of this study was to evaluate if the bovine dentin barrier produces similar results to the human dentin barrier in assessing the transdentinal cytotoxicity of resin luting cements. The study found that bovine dentin had larger dentinal tubule diameters, but similar numbers of tubules. Additionally, there was no difference in cytotoxicity when the same luting cement was applied on human or bovine dentin barriers.
JOURNAL OF PROSTHETIC DENTISTRY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Igor Paulino Mendes Soares, Caroline Anselmi, Maria Luiza Barucci Araujo Pires, Rafael Antonio de Oliveira Ribeiro, Maria Luisa Leite, Diana Gabriela Soares, Carlos Alberto De Souza Costa, Josimeri Hebling
Summary: Simulating a pulpitis-like condition by exposing dental pulp cells to lipopolysaccharides can help explore mechanisms and bioactive molecules to counteract the adverse effects. The exposure to 10 μg/mL LPS for seven days impaired the odontogenic potential of HDPCs in vitro.
JOURNAL OF APPLIED ORAL SCIENCE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Marjorie de Oliveira Gallinari, Ester Alves Ferreira Bordini, Vitor de Toledo Stuani, Fernanda Balestrero Cassiano, Camila Correa da Silva Braga de Melo, Juliano Milanezi de Almeida, Luciano Tavares Angelo Cintra, Carlos Alberto De Souza Costa, Diana Gabriela Soares
Summary: This study evaluated the bioactive potential of a macro-porous chitosan scaffold incorporated with calcium hydroxide (CH-Ca) and functionalized with bioactive doses of simvastatin (SV) for bone tissue regeneration. The results showed that the CH-Ca-SV scaffold exhibited the most intense bio-stimulating effect, leading to increased deposition of mineralized matrix.
BRAZILIAN ORAL RESEARCH
(2023)
