Journal
CHEMOSPHERE
Volume 220, Issue -, Pages 362-370Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2018.12.122
Keywords
Biological effect; Bisphenol AF; Estrogen receptor alpha; G protein-coupled receptor; MCF-7 cells
Categories
Funding
- National Natural Science Foundation of China [21507078, 21777093, 41430644, 41373098]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT13078]
- Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program [2017BT01Z032]
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The negative health effects of bisphenol A (BPA) due to its estrogenic activity result in the increasing usage of alternative bisphenols (BPs) including bisphenol AF (BPAF). To comprehensive understand health effects of BPAF, the MCF-7 cells were used to investigate the effects of BPAF on cell proliferation, intracellular reactive oxygen species (ROS) formation, and calcium ion (Ca2+) level. The molecular mechanisms of cell biological responses caused by BPAF were investigated by analyzing target protein expression. The results showed that low-concentration BPAF induces significant effects on MCF-7 cells, including promoting cell proliferation and elevating intracellular ROS and Ca2+ levels. BPAF in low concentration significantly enhances the protein expression of estrogen receptor a (ERa), G protein coupled receptor (GPER), c-Myc, and Cyclin D1, as well as increases phosphorylation levels of protein kinase B (Akt) and extracellular signal-regulated kinase (Erk) in MCF-7 cells. After the addition of ERa, GPER, and phosphatidylinositide 3-kinase (PI3K) inhibitors, phosphorylations of Erk and Akt were both inhibited. In addition, specific signal inhibitors significantly attenuated the effects of BPAF. Silencing of GPER also markedly decreased BPAF induced cell proliferation. The present results suggested that BPAF can activate PI3K/Akt and Erk signals via GPER, which, in turn, stimulate cellular biological effects induced by BPAF. ERa also plays a critical role in BPAF induced cellular biological effects. (C) 2018 Elsevier Ltd. All rights reserved.
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