Journal
CARBOHYDRATE POLYMERS
Volume 211, Issue -, Pages 57-68Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2019.01.108
Keywords
Carbohydrate binding modules; Lignocellulosic biomass; Cellulose hydrolysis; Cellulases
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Funding
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2015/13684-0, 2010/18773-8]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [405191/2015-4, 140667/2015-6, 158752/2015-5, 303988/2016-9, 440977/2016-9]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [15/13684-0] Funding Source: FAPESP
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Plant biomass is a low-cost and abundant source of carbohydrates for production of fuels, green chemicals and materials. Currently, biochemical conversion of the biomass into sugars via enzymatic hydrolysis is the most viable technology. Here, the role of carbohydrate binding modules (CBMs) in the disruption of insoluble polysaccharide structures and their capacity to enhance cellulase-promoted lignocellulosic biomass hydrolysis was investigated. We show that CBM addition promotes generation of additional reducing ends in the insoluble substrate by cellulases. On the contrary, bovine serum albumin (BSA), widely used in prevention of a nonspecific protein binding, causes an increase in soluble reducing-end production, when applied jointly with cellulases. We demonstrate that binding of CBMs to cellulose is non-homogeneous, irreversible and leads to its amorphisation. Our results also reveal effects of CBM-promoted amorphogenesis on cellulose hydrolysis by cellulases.
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