Review
Biochemistry & Molecular Biology
Oxana Yu Kytikova, Tatyana P. Novgorodtseva, Yulia K. Denisenko, Denis E. Naumov, Tatyana A. Gvozdenko, Juliy M. Perelman
Summary: Asthma is a chronic disease with a complex etiology, influenced by both natural-climatic and anthropogenic factors. Studies have shown that temperature plays a significant role in airway inflammation, and thermosensory TRP channels are crucial in the pathogenesis of asthma. New treatment methods for asthma exacerbations caused by temperature and humidity should focus on regulating channel activity.
Article
Biochemistry & Molecular Biology
Marialuisa Aragona, Caterina Porcino, Marilena Briglia, Kamel Mhalhel, Francesco Abbate, Maria Levanti, Giuseppe Montalbano, Rosaria Laura, Eugenia Rita Lauriano, Antonino Germana, Maria Cristina Guerrera
Summary: This study reveals the presence of Vimentin RV202 in taste bud cells of zebrafish for the first time. Vimentin RV202 may play an important role in the maturation and regeneration of taste bud cells. Further research is needed to elucidate its synergistic work with ubiquitin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Claudia Gatta, Valentina Schiano, Chiara Attanasio, Carla Lucini, Antonio Palladino
Summary: In this study, the presence of neurotrophic factors in the taste bud cells of zebrafish is demonstrated. These neurotrophins play an important role in taste bud function and share similarities with those found in mammals. This study further confirms the significance of the zebrafish model in translational research.
Review
Agriculture, Dairy & Animal Science
Giuseppe Montalbano, Maria Levanti, Kamel Mhalhel, Francesco Abbate, Rosaria Laura, Maria Cristina Guerrera, Marialuisa Aragona, Antonino Germana
Summary: Zebrafish serves as an important experimental model for studying various diseases. This review summarizes the presence and function of ASICs in zebrafish, revealing their expression and roles in sensory organs.
Article
Pharmacology & Pharmacy
Xiao-Yu Cheng, Shu-Fang Li, Yong Chen, Ying-Jie Zhao, Wei Hu, Chao Lu, Ren-Peng Zhou
Summary: TRPM7 is a channel protein located on the cell membrane that regulates the balance of intracellular Mg2+. It plays an important role in cell proliferation, adhesion, migration, and is directly related to disease occurrence and development.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Review
Behavioral Sciences
Qin'yi Xu, Yan Zou, Zeng'li Miao, Lei Jiang, Xu'dong Zhao
Summary: Stroke is a major cause of death and disability, with a rising incidence in developing countries. Transient receptor potential ion channels, expressed in the brain, have been implicated in the pathophysiology of stroke, although the exact mechanisms are still unclear.
BRAIN AND BEHAVIOR
(2023)
Article
Behavioral Sciences
Isaac A. Adedara, Fabiano V. Costa, Eduarda Biasuz, Julia Canzian, Ebenezer O. Farombi, Denis B. Rosemberg
Summary: Amiloride, a nonselective ASIC blocker, was found to negatively modulate specific behavioral responses in a zebrafish model of acute visceral pain by attenuating acetic acid-induced pain and preventing aberrant behaviors associated with impaired locomotion.
BEHAVIOURAL BRAIN RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Charlotte Van den Eynde, Joris Vriens, Katrien De Clercq
Summary: Calcium ions play a critical role in cell function, with transient receptor potential (TRP) channels serving as a type of ion channel that can convey various stimuli and potentially participate in cellular responses to changing environments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Review
Chemistry, Medicinal
Murat Oz, Dietrich E. Lorke, Frank C. Howarth
Summary: Capsaicin, derived from chili pepper, has various pharmacological actions, including analgesic, anticancer, anti-inflammatory, antiobesity, and antioxidant effects. It acts on not only TRPV1 receptor, but also other ion channels and enzymes. This study categorizes the non-TRPV1 targets of capsaicin and discusses the cellular and molecular mechanisms of its TRPV1-independent effects.
MEDICINAL RESEARCH REVIEWS
(2023)
Article
Agriculture, Dairy & Animal Science
Yuta Yoshida, Fuminori Kawabata, Shoji Tabata, Samuel E. Aggrey, Romdhane Rekaya, Hong-Xiang Liu
Summary: Recent studies have shown that modern high-yielding broilers exhibit higher sensitivity to bitter taste stimuli compared to traditional meat-type chicken strains. This evolution of taste sensitivities may be associated with alterations in the upper level of the taste system.
Article
Agriculture, Dairy & Animal Science
Karina Lezama-Garcia, Daniel Mota-Rojas, Alfredo M. F. Pereira, Julio Martinez-Burnes, Marcelo Ghezzi, Adriana Dominguez, Jocelyn Gomez, Ana de Mira Geraldo, Pamela Lendez, Ismael Hernandez-Avalos, Isabel Falcon, Adriana Olmos-Hernandez, Dehua Wang
Summary: This review discusses recent discoveries about transient receptor potential and their role in thermoregulatory mechanisms of animals, emphasizing the importance of understanding how these receptors are activated and the pathways they use to recognize specific thermal sensations. Ongoing research aims to develop therapeutic approaches targeting these receptors to improve non-human animals' quality of life. The review presents and analyzes findings on TRP structure, physiology, and neurotransmission in thermoregulation, highlighting the need for further studies to advance our understanding of these mechanisms and control thermoregulatory disorders.
Article
Cell Biology
Makoto Ohmoto, Satsuki Kitamoto, Junji Hirota
Summary: Taste substances are detected by taste receptor cells in the oral epithelium, which are continuously replaced by new ones generated from local epithelial stem cells. Pou2f3 is a key transcription factor that regulates the generation and differentiation of sweet, umami, and bitter cells. Through RNA-sequence data analysis, Eya1 was identified as the only transcription factor gene showing higher expression in bitter cells than in sweet cells, suggesting it plays a role in regulating the generation and differentiation of bitter cells.
CELL AND TISSUE RESEARCH
(2021)
Review
Cell Biology
Theodoros Rizopoulos, Martha Assimakopoulou
Summary: TRPs play a crucial role in the pathogenesis of colorectal cancer and could serve as potential therapeutic targets and prognostic biomarkers.
HISTOLOGY AND HISTOPATHOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Nina Ruan, Jacob Tribble, Andrew M. Peterson, Qian Jiang, John Q. Wang, Xiang-Ping Chu
Summary: ASICs are proton-gated cation channels activated by pH drops and nonproton ligands, playing crucial roles in various physiological and pathological processes. They are predominantly expressed in the central nervous system and have been linked to functions such as synaptic plasticity, learning/memory, and mechanosensation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Physiology
Lin Ma, Xin Liu, Qing Liu, Sen Jin, Heng Chang, Haixia Liu
Summary: Transient receptor ion potential (TRP) channels are non-selective cation channels that play important roles in regulating cellular functions and signaling pathways. They have been extensively studied in the context of glaucoma, where various TRP subfamilies have been found to be abundantly expressed in ocular structures. Understanding the potential role of TRP channels in glaucoma progression could lead to the identification of new therapeutic targets.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Anatomy & Morphology
Francesco Abbate, Maria Cristina Guerrera, Maria Levanti, Rosaria Laura, Marialuisa Aragona, Kamel Mhalhel, Giuseppe Montalbano, Antonino Germana
Summary: This study described the morphological characteristics of the tongue in the blackspot seabream, revealing structures such as teeth, taste pores, papillae, and mesenchymal stem cells. The results suggest the potential for wider use of blackspot seabream in aquaculture, as well as providing a basis for future feeding strategies and clinical trials.
ANATOMIA HISTOLOGIA EMBRYOLOGIA
(2022)
Article
Fisheries
Matteo Zarantoniello, Lina Fernanda Pulido Rodriguez, Basilio Randazzo, Gloriana Cardinaletti, Elisabetta Giorgini, Alessia Belloni, Giulia Secci, Filippo Faccenda, Domitilla Pulcini, Giuliana Parisi, Fabrizio Capoccioni, Emilio Tibaldi, Ike Olivotto
Summary: Using functional feed additives and new feed supplements can improve growth performance, gut health, and immune response in fish. The study found that TS and RCM diets had better effects, although they slightly worsened the zootechnical parameters of the fish, they were able to improve overall welfare and preserve the structural integrity of the intestine.
Article
Fisheries
Giulia Chemello, Matteo Zarantoniello, Basilio Randazzo, Giorgia Gioacchini, Cristina Truzzi, Gloriana Cardinaletti, Paola Riolo, Ike Olivotto
Summary: The study found that replacing up to 50% of fishmeal with enriched Black Soldier Fly prepupae meal did not impact the physiological responses of zebrafish, but higher replacement levels (75% and 100%) negatively affected fish stress response, oocyte maturation stages, spawning, and hatching success.
Article
Biochemistry & Molecular Biology
Marialuisa Aragona, Caterina Porcino, Maria Cristina Guerrera, Giuseppe Montalbano, Rosaria Laura, Marzio Cometa, Maria Levanti, Francesco Abbate, Teresa Cobo, Gabriel Capitelli, Jose A. Vega, Antonino Germana
Summary: This review provides an update on the distribution and functions of the BDNF-TrkB system in the sensory organs of zebrafish, highlighting its significance in fish.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Basilio Randazzo, Matteo Zarantoniello, Giulia Secci, Filippo Faccenda, Francesca Fava, Giulia Marzorati, Alessia Belloni, Francesca Maradonna, Veronica Orazi, Roberto Cerri, Michele Povinelli, Giuliana Parisi, Elisabetta Giorgini, Ike Olivotto
Summary: Carpione (Salmo carpio, Linnaeus 1758) is an endangered endemic fish species in Lake Garda, Italy. This study aimed to investigate the effects of different diets on the growth, health, and nutrient composition of Carpione. Four experimental diets, including a control diet based on marine ingredients and three diets with varying proportions of vegetable ingredients or processed animal proteins, were tested over a three-month feeding trial. The results showed that there were no significant differences in growth performance among the experimental groups. However, histological alterations and inflammation markers were observed in the group fed with a diet containing high proportions of vegetable ingredients. The inclusion of processed animal proteins improved gut health and nutrient uptake but also led to changes in the fatty acid composition of the fish fillets. Further research is needed to maintain the quality traits of Carpione while promoting its health and growth.
Article
Biochemistry & Molecular Biology
Alessia Belloni, Michele Furlani, Stefania Greco, Valentina Notarstefano, Chiara Pro, Basilio Randazzo, Pamela Pellegrino, Alessandro Zannotti, Giovanni Delli Carpini, Andrea Ciavattini, Francesca Di Lillo, Elisabetta Giorgini, Alessandra Giuliani, Saverio Cinti, Pasquapina Ciarmela
Summary: Collagen is an important component of the extracellular matrix and plays a crucial role in maintaining tissue support. In fibrotic diseases, collagen is overexpressed, leading to the formation of a stiffer extracellular matrix. By using high-resolution analytical tools, it is possible to investigate the three-dimensional organization and secondary structure of collagen in fibrotic diseases, which can help identify specific markers associated with pathological conditions. In this study, a multidisciplinary approach using Phase-Contrast MicroComputed Tomography, Transmission Electron Microscopy, and Fourier Transform Infrared Imaging Spectroscopy was applied to analyze the microstructural features of collagen in leiomyoma samples and adjacent myometrium. Leiomyoma is a common gynecological disorder characterized by excessive collagen production. Additionally, the effects of omega-3 fatty acids EPA and DHA on collagen reduction were also investigated.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Caterina Porcino, Marilena Briglia, Marialuisa Aragona, Kamel Mhalhel, Rosaria Laura, Maria Levanti, Francesco Abbate, Giuseppe Montalbano, Germana Germana, Eugenia Rita Lauriano, Alessandro Meduri, Jose Antonio Vega, Antonino Germana, Maria Cristina Guerrera
Summary: The increasing incidence rates of light-induced retinopathies can be attributed to continuous exposure to light from various electronic devices. Studies have shown a relation between blue light exposure and the pathogenesis of these retinopathies, but the specific pathophysiological mechanisms are still unknown. This study focuses on the effects of light exposure at different wavelengths in the blue light range on the distribution of calcium-binding proteins CaB-D28K and CaR-N18 in the adult zebrafish retina, providing insights into their potential role in maintaining retinal homeostasis under different light conditions and darkness.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Eugenia Rita Lauriano, Alessio Alesci, Marialuisa Aragona, Simona Pergolizzi, Anthea Miller, Kristina Zuwala, Michal Kuciel, Giacomo Zaccone, Antonino Germana, Maria Cristina Guerrera
Summary: This study characterizes the leukocytes present in the gut-associated lymphoid tissue (GALT) of H. niloticus for the first time using confocal immuno-fluorescence techniques. The results show massive aggregates of immune cells in the submucosal thickness, forming oval-shaped structures similar to isolated lymphoid follicles found in birds and mammals. This expands our understanding of intestinal immunity in this fish.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Veterinary Sciences
Nicola F. Addeo, Basilio Randazzo, Ike Olivotto, Maria Messina, Francesca Tulli, Simone Vozzo, Youssef A. Attia, Tiziana M. Mahayri, Francesco Iannaccone, Khalid A. Asiry, Giuseppe Moniello, Fulvia Bovera
Summary: This study investigated the effects of adding Tenebrio molitor larvae meal (TML) to the diet of laying Japanese quails. The results showed that the inclusion of TML at 1.4% improved the intestinal health of the birds, as indicated by increased villi height and decreased crypt depth.
RESEARCH IN VETERINARY SCIENCE
(2022)
Review
Cell Biology
Kamel Mhalhel, Mirea Sicari, Lidia Pansera, Jincan Chen, Maria Levanti, Nicolas Diotel, Sepand Rastegar, Antonino Germana, Giuseppe Montalbano
Summary: In the past century, advancements in biotechnology, biochemistry, and pharmacognosy have brought attention to flavonoids, which are polyphenolic secondary metabolites capable of modulating various biological mechanisms, including those involved in neuronal plasticity, learning, and memory. Flavonoids also impact biological processes associated with neurodegenerative diseases such as oxidative stress, neuroinflammation, and mitochondrial dysfunction. Therefore, certain flavonoids can be used as adjuvants to prevent and counteract disorders like Alzheimer's and Parkinson's diseases. Zebrafish, with its high genome homology to humans, similar brain organization and neurochemical processes, and maintained neurogenic activity in the adult brain, provides a valuable model for studying human neurodegenerative diseases and the effects of flavonoids on these disorders.
Article
Biology
Marialuisa Aragona, Marilena Briglia, Caterina Porcino, Kamel Mhalhel, Marzio Cometa, Patrizia Germana Germana, Giuseppe Montalbano, Maria Levanti, Rosaria Laura, Francesco Abbate, Antonino Germana, Maria Cristina Guerrera
Summary: Calcium-binding proteins play a crucial role in the survival of neurons by buffering intracellular calcium ion concentration and defending against toxic increases. This study discovered the presence of several calcium-binding proteins in the retina of Nothobranchius guentheri, suggesting its suitability as a model for studying changes in calcium-binding protein expression patterns in neurodegenerative processes related to the retina.
Article
Fisheries
Giacomo Zaccone, Alessio Alesci, Doaa M. M. Mokhtar, Marialuisa Aragona, Maria Cristina Guerrera, Gioele Capillo, Marco Albano, Jorge de Oliveira Fernandes, Viswanath Kiron, Ramy K. A. Sayed, Marwa M. M. Hussein, Patrizia Lo Cascio, Michal Kuciel, Krystyna Zuwala, Antonino Germana, Jose Manuel Icardo, Eugenia Rita Lauriano
Summary: The recognition and elimination of invading pathogens are essential for host survival. Macrophages, which function similarly to vertebrate macrophages, play a crucial role in host protection in all multicellular organisms. CD14 is a pattern responsible for bacterial recognition found on the surface of macrophages. These cells store antimicrobial molecules and display polarization states similar to mammalian macrophages. The present study reports the presence of various cell markers, including CD14, in the intestinal macrophages of catfish and bonytongue, providing insights into the in vivo function of macrophages in fish.
Article
Biochemistry & Molecular Biology
Alessia Belloni, Michele Furlani, Stefania Greco, Valentina Notarstefano, Chiara Pro, Basilio Randazzo, Pamela Pellegrino, Alessandro Zannotti, Giovanni Delli Carpini, Andrea Ciavattini, Francesca Di Lillo, Elisabetta Giorgini, Alessandra Giuliani, Saverio Cinti, Pasquapina Ciarmela
Summary: Collagen, as a crucial component of the extracellular matrix, plays a key role in tissue structural support, particularly in fibrotic diseases. Investigating collagen structure can help identify specific pathological markers. Uterine leiomyoma serves as a valuable model to study collagen self-organization in a pathological condition using innovative multidisciplinary approaches.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Anatomy & Morphology
Rongyan Sun, Yubo Zhou, Jiao Liang, Lihong Yang, Zhengjun Fan, Huali Wang
Summary: This study found that knockdown of MDM2 can attenuate vascular endothelial dysfunction in hypertension, which may be achieved through inhibiting the activation of Notch1 and NLRP3 inflammasome.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2024)
Article
Anatomy & Morphology
E. M. Fernandez-Rubio, R. J. Radlanski
Summary: This study investigates the development of the primary and secondary temporomandibular joints in humans, focusing on their coexistence and subsequent separation. The findings reveal that both joints develop simultaneously and remain connected for over 6 weeks before separating at around the 18th to 24th week. Understanding the precise timing and functional movements involved in the development of these joints is crucial for comprehending the overall development of the temporomandibular joint.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2024)
Article
Anatomy & Morphology
Nawwaf Sebastian Damen, Sorin Hostiuc, Adelina Maria Jianu, Bogdan Adrian Manta, Mugurel Constantin Rusu, Mihai Adrian Dobra
Summary: This study documented the anatomical variations of the left renal vein (LRV) through a retrospective analysis of 375 computed tomography angiograms. Five different anatomical types of LRV were identified, and a new anatomical classification system for the retroaortic LRV (RLRV) was proposed.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2024)
Article
Anatomy & Morphology
Harald Knof, Markus Berndt, Thomas Shiozawa
Summary: This study investigated the impact of online learning conditions on the oral exam performance of medical students. The results showed that students who used collaborative learning strategies were able to assess their abilities more realistically. However, the learning strategies did not have an impact on exam results.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2024)
Review
Anatomy & Morphology
Felix Margenfeld, Adib Zendehdel, Giorgio Tamborrini, Magdalena Mueller-Gerbl
Summary: This study is the first scoping review to screen ultrasound studies on human cadaver joints and revealed a heterogeneous field. It is recommended to use a uniform method for conducting and presenting ultrasound examinations in future studies.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2024)
Article
Anatomy & Morphology
Rafael Heiss, Svenja A. Hoeger, Michael Uder, Thilo Hotfiel, Jannis Hanspach, Frederik B. Laun, Armin M. Nagel, Frank W. Roemer
Summary: This study utilized 7 Tesla magnetic resonance imaging (MRI) to assess morphological and functional changes in skeletal muscle during exercise-induced delayed onset muscle soreness (DOMS). The results showed significant alterations in T2 signal intensity and T2 values immediately after exercise and 60 hours later. These findings suggest the potential use of ultra-high field MRI in sports medicine to monitor microstructural muscle damage and guide appropriate therapeutic strategies.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2024)
Article
Anatomy & Morphology
Lukasz Olewnik, Nicol Zielinska, Kacper Ruzik, Michal Podgorski, Przemyslaw Kicinski, Rui Diogo, Friedrich Paulsen
Summary: This study provides the first comprehensive classification of the fibularis brevis tendon based on its insertion in human fetuses and evaluates the prevalence of fibularis digiti quinti.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2024)
Article
Anatomy & Morphology
Denise Bonente, Virginia Barone, Vitaliano Francesco Muzii, Sara Ottolenghi, Miriam Durante, Sandra Bracco, Claudio Nicoletti, Eugenio Bertelli
Summary: This study characterizes the morphology and topography of the spina musculi recti lateralis (SMRL) and compares it to known bony landmarks. The results show that the SMRL is a process of the sphenoidal orbital plate and serves as a reliable landmark for the insertion of the tendinous ring and lateral rectus. This is an important variation for orbital surgeons to be aware of.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2024)
Article
Anatomy & Morphology
Fatih Cicek, Selim Cinaroglu, Faruk Gazi Ceranoglu, Erkut Kucuk, Kursad Ramazan Zor
Summary: This study revealed the nerve innervation of the orbicularis oculi muscle for BoNT treatment of blepharospasm using Sihler's staining technique. It identified precise crossing points of these nerves, which can be targeted for BoNT administration, leading to more efficient outcomes and reduced expenses.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2024)