Article
Biotechnology & Applied Microbiology
R. Gathercole, E. Tranfield, D. Xia, G. Perez-Cordon, G. Robinson, D. Timofte, F. Zendri, R. M. Chalmers
Summary: The study developed a method to purify Cryptosporidium spp. oocysts from clinical stool samples and evaluated them using mass spectrometry. The method produced distinct spectra specific to different Cryptosporidium species, demonstrating the utility of the purification method.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Kristine Korzow Richter, Maria C. Codlin, Melina Seabrook, Christina Warinner
Summary: Collagen peptide mass fingerprinting by MALDI-TOF-MS, also known as ZooMS, is a rapidly growing analytical technique in the fields of archaeology, ecology, and cultural heritage. It enables rapid taxonomic identification of large bone assemblages and cultural heritage objects. However, there are limitations that need to be addressed for future advancements.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Anil K. Persad, Hanan A. Fahmy, Nicholas Anderson, Jing Cui, Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M. Spanninger, Robert L. Buchanan, Kalmia E. Kniel, Michele T. Jay-Russell, Michelle D. Danyluk, Gireesh Rajashekara, Jeffrey T. LeJeune
Summary: This study demonstrates that MALDI-TOF is a viable alternative for the rapid identification and differentiation of Salmonella serovars.
Article
Multidisciplinary Sciences
Natalija Topic Popovic, Slavko Kepec, Snjezana P. Kazazic, Ivancica Strunjak-Perovic, Krunoslav Bojanic, Rozelindra Coz-Rakovac
Summary: This study evaluated the use of the API20E biochemical identification system as a supportive tool for enhancing the discrimination of environmental bacteria by the MALDI-TOF MS method. The results showed that the API20E system can effectively identify bacterial genera, but there are issues with misidentification. In environmental isolates with lower identification scores, the API20E tests can be used as a purposeful and inexpensive identification support.
Article
Microbiology
Camila Morschbacher Wilhelm, Maiara dos Santos Carneiro, Everton Inamine, Afonso Luis Barth
Summary: This study aimed to evaluate an adaptation of the MBT-ASTRA technique for faster antimicrobial susceptibility testing. The adapted method showed high categorical agreement with the standard method, indicating its potential for rapid AST results.
MICROBIOLOGY SPECTRUM
(2023)
Article
Medical Laboratory Technology
Qianqian Zhang, Ge Wang, Dehui Sun, Wanying Lin, Tizhen Yan, Yuanjun Wu, Meiying Wu, Jianhong Chen, Shaomin Zou, Wenchun Xie, Yuqiu Zhou, Yuxi Wang, Linlin He, Yanhui Liu, Zhenxiong Qiu, Lingling Hu, Bin Lin, Xiaoguang Zhou, Yan Li, Xiangmin Xu
Summary: A MALDI-TOF-MS assay was developed for identifying various beta-globin disorders and classifying thalassemia major (TM) and thalassemia intermedia (TI) patients. The assay showed comparable accuracy to traditional methods but higher clinical sensitivity. It also identified additional abnormal hemoglobins and accurately screened for carriers of deletional hereditary persistence of fetal hemoglobin or delta beta-thalassemia. The study concludes that MALDI-TOF-MS is a highly accurate and predictive tool for large-scale screening and clinical classification of beta-globin disorders.
CLINICAL CHEMISTRY
(2022)
Article
Dermatology
Dipika Shaw, Anup K. Ghosh, Saikat Paul, Shreya Singh, Arunaloke Chakrabarti, Harsimran Kaur, Tarun Narang, Sunil Dogra, Shivaprakash M. Rudramurthy
Summary: This study developed and validated an efficient protein extraction protocol for the accurate identification of dermatophytes using MALDI-TOF MS. The protocol provided high accuracy and comparable results to DNA sequence-based identification.
Article
Chemistry, Analytical
Stefania-Alexandra Iakab, Gerard Baquer, Marta Lafuente, Maria Pilar Pina, Jose Luis Ramirez, Pere Rafols, Xavier Correig-Blanchar, Maria Garcia-Altares
Summary: This study presents a multimodal imaging approach that combines surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) and surface-enhanced Raman spectroscopy (SERS) using a gold-coated nanostructured silicon substrate. By using the same substrate, sample preparation, and data analysis software, both imaging modalities can be integrated and co-registered on the same sample. The approach was successfully applied to analyze the chemical composition and distribution of molecules transferred from clean fingertips and fingertips covered with plasticine modeling clay, providing valuable information on the composition of the clay and locating the traces of plasticine on fingermarks.
ANALYTICAL CHEMISTRY
(2022)
Article
Infectious Diseases
Emilie Cardot Martin, Claudine Renaux, Emilie Catherinot, Lucie Limousin, Louis Jean Couderc, Marc Vasse
Summary: This study evaluated the new ID-FUNGI plate for mold identification using MALDI Biotyper, and found that IDFP significantly improved species identification compared to traditional Sabouraud agar with gentamicin and chloramphenicol. The results of the study suggest that the new IDFP can enhance mycological diagnostic accuracy and workflow in laboratories.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Agriculture, Multidisciplinary
Liang Shan, Yiman Huang, Jing Zhang, Yue Su, Yinlong Guo
Summary: In this study, the use of cellulose nanocrystal (CNC) was shown to effectively reduce protein aggregation in the sample solution, leading to improved sensitivity and repeatability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis for intact protein. The addition of CNC resulted in a 2-fold increase in the signal-to-noise ratio and a detection limit of <10 µg/mL for intact protein. Furthermore, the use of CNC improved the repeatability of ion signals and demonstrated a linear relationship between protein ion intensity and concentration.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Applied
Yunhe Hong, Nicholas Birse, Brian Quinn, Yicong Li, Wenyang Jia, Saskia van Ruth, Christopher T. Elliott
Summary: In this study, the difference between wild and farmed salmon production was successfully characterized using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) combined with chemometric analysis. The established method involved multivariate analysis techniques, such as principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and orthogonal partial least squares-discriminant analysis (OPLS-DA), to identify distinctive features in wild and farmed salmon products. The proposed method achieved 100% classification accuracy in discriminating farmed and wild salmon, as well as distinguishing different species of wild salmon. The results suggest that this method could be used as a reference for detecting salmon fraud related to production type and expand the application of MALDI-ToF technology in food authenticity testing.
Article
Microbiology
Arnaud Jabet, Anne-Cecile Normand, Alicia Moreno-Sabater, Jacques Guillot, Veronica Risco-Castillo, Sophie Brun, Magalie Demar, Romain Blaizot, Cecile Nabet, Ann Packeu, Renaud Piarroux
Summary: The objective of the study was to reduce recurrent errors between phylogenetically close species by adding new reference spectra. Diversifying the references and adding spectra from various culture media and culture durations improved identification performance, although confusion remained between certain closely related species.
Article
Food Science & Technology
Minghui Yan, Liwen Luo, Dandan Li, Zhenmin Liu, Ran Wei, Jia Yi, Liang Qiao, Chunping You
Summary: We developed an efficient strategy to assess the risk of biofilm formation by psychrotrophic Pseudomonas in raw milk using MALDI-TOF MS. By collecting molecular mass fingerprints of whole cells and combining multivariate statistical analysis with machine learning, we established a classification model for the biofilm-forming ability of psychrotrophs. The results showed that different biofilm-forming capabilities of Pseudomonas can be well distinguished by the MALDI-TOF MS molecular mass fingerprints. The established classification model demonstrated 100% accuracy in identifying biofilm-forming risk.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Infectious Diseases
T. Garrigos, C. Neuwirth, A. Chapuis, J. Bador, L. Amoureux
Summary: The study aimed to establish a MALDI-TOF/MS Bruker database including 18 Achromobacter species and evaluate its suitability in laboratory settings. The new database accurately identified 99.4% of isolates, significantly outperforming the Bruker default database at 50.9%. This highlights the potential of using MALDI-TOF/MS with a local database for rapid and accurate identification.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Retraction
Chemistry, Multidisciplinary
Feng Ding, Yuna Qian, Zaian Deng, Jitai Zhang, Yongchao Zhou, Lan Yang, Fangyan Wang, Juping Wang, Zhihua Zhou, Jianliang Shen
Summary: The study on size-selected silver nanoparticles for MALDI-TOF mass spectrometry of amyloid-beta peptides by Feng Ding et al. was retracted from Nanoscale journal in 2018.
Article
Chemistry, Organic
Sandeep Singh, Annah Gupta, Kamal K. Kapoor
SYNTHETIC COMMUNICATIONS
(2020)
Article
Chemistry, Organic
Sheena Mahajan, Nancy Slathia, Kamal K. Kapoor
TETRAHEDRON LETTERS
(2020)
Article
Chemistry, Multidisciplinary
Rajneesh P. Sharma, Usha K. Verma, Kamal K. Kapoor
Article
Chemistry, Physical
Yeshwinder Saini, Sheena Mahajan, Kamal K. Kapoor, Geeta Hundal, Saikat Kumar Seth
Summary: Three ninhydrin derivatives (2-4) were synthesized and characterized by single crystal X-ray diffraction. The solid-state structures of these compounds show various intermolecular interactions, including C-H ... π, π-π, and lone-pair ... π interactions. The analysis through Hirshfeld surface reveals both similarities and differences in these interactions among the compounds.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Chemistry, Organic
Nancy Slathia, Annah Gupta, Kamal K. Kapoor
Summary: The iodine/TBHP/DMSO mediated oxidative rearrangement of 3-styrylquinoxalin-2(1H)-one led to the formation of 3-aroylquinoxalin-2(1H)-ones in good to high yields via Kornblum oxidation. This methodology proceeds under mild conditions via oxidative aryl migration, followed by C-C bond cleavage and does not make use of any metal.
TETRAHEDRON LETTERS
(2021)
Article
Chemistry, Analytical
Shahid Shafi Shah, Dinesh Kumar, Taniya Sharma, Kamal K. Kapoor, Rajinder K. Bamezai
Summary: In this study, chemically treated walnut leaves were used as adsorbents for crystal violet dye removal from aqueous solution. Various characterization techniques were used to analyze the adsorbents. The adsorption process was found to be most efficient under specific conditions, with Freundlich isotherm fitting well and the kinetics following pseudo-second-order rate equation. The Gibbs free energy was determined to be spontaneous, and response surface methodology and density functional theory were applied for further analysis and optimization.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Organic
Lalita Devi, Annah Gupta, Kamal K. Kapoor
Summary: Barium chloride embedded polyaniline (Ba/PANI) nanocomposite has been used as an efficient heterogeneous nanocatalyst for the synthesis of biologically active quinoxalinone compounds. The results demonstrate that Ba/PANI nanocatalyst exhibits significantly higher catalytic activity compared to BaCl2 and PANI, and it can be easily recovered and reused. The synthesized nanocomposite has been characterized using various techniques.
POLYCYCLIC AROMATIC COMPOUNDS
(2023)
Article
Chemistry, Organic
Varun Sharma, Nancy Slathia, Sheena Mahajan, Kamal K. Kapoor, Vivek Kumar Gupta
Summary: A novel biologically active heterocyclic compound has been synthesized and characterized through various analytical techniques. The stable crystal structure showed complex intermolecular interactions and exhibited promising potential for anti-Alzheimer's activity.
POLYCYCLIC AROMATIC COMPOUNDS
(2022)
Article
Chemistry, Multidisciplinary
Venu Sharma, Arem Qayum, Kamal K. Kapoor, Debaraj Mukherjee, Shashank K. Singh, Manoj K. Dhar, Sanjana Kaul
Summary: This study described the synthesis of 14-deoxy-benzylidene-8,17-epoxy-diene-andrographolide derivatives from andrographolide and evaluated their anticancer activities. The synthesized compounds showed significant proliferation inhibition against various human cancer cell lines.
JOURNAL OF THE INDIAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Organic
Rajneesh Paul Sharma, Sheena Mahajan, Nancy Slathia, Kamal K. Kapoor
Summary: The synthesis of (E)-3-substituted styrylquinoxalin-2(1H)-ones was achieved by the reaction of 3-methylquinoxalinone and aromatic aldehydes using FeCl3 as a catalyst in toluene under microwave irradiation. The protocol is characterized by milder reaction conditions, high yields of product, and easy workup procedures.
SYNTHETIC COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Sonakshi Sasan, Tavishi Chopra, Annah Gupta, Dolma Tsering, Kamal K. Kapoor, Raman Parkesh
Summary: Two cyanoimidazopyridine-based sensors (SS1 and SS2) have been studied for the colorimetric and fluorometric detection of Fe2+, Fe3+, and Cu2+ ions in a semi-aqueous medium. The sensors show a turn-off fluorescence response due to the restriction in internal charge transfer. The binding ratios of SS1 and SS2 with Cu2+ and Fe2+/3+ ions have been determined, and the sensors exhibit high binding constant values and low detection limits. Additionally, sensor-coated filter paper strips have been prepared for real-time detection of the ions based on color changes.
Article
Biochemistry & Molecular Biology
Venu Sharma, Supriya Sharma, Rukmankesh Mehra, Kamal K. Kapoor, Manoj K. Dhar, Sanjana Kaul
Summary: This study explored the antibacterial activity of a glucoside labdane 'neoandrographolide' and its derivatives, which were modified from a natural product. The results showed promising potential as antibacterial agents against drug-resistant pathogens.
INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS
(2022)
Article
Chemistry, Multidisciplinary
Mahesh K. Zilla, Sheena Mahajan, Rajni Khajuria, Vivek K. Gupta, Kamal K. Kapoor, Asif Ali
Summary: A mild and efficient synthesis method was reported for the preparation of various 4-phenoxyquinazoline, 2-phenoxyquinoxaline, and 2-phenoxypyridine derivatives from specific starting materials. The synthetic approach offers a new environmentally friendly way to access these compounds with high yields and broad substrate scope.
Article
Chemistry, Multidisciplinary
Dilpreet Kour, Sonakshi Sasan, Kamal K. Kapoor
JOURNAL OF CHEMICAL SCIENCES
(2019)
Article
Chemistry, Multidisciplinary
Sheena Mahajan, Nancy Slathia, Vijay K. Nuthakki, Sandip B. Bharate, Kamal K. Kapoor
