Article
Microbiology
Joshua R. Prindle, Yibo Wang, Julian M. Rocha, Andreas Diepold, Andreas Gahlmann
Summary: Injectisome proteins in the cytosol form distinct complexes with each other prior to binding to injectisomes, and these interactions have important implications for the functional regulation of injectisomes.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Multidisciplinary
Ryo Kawaguchi, Katsushi Hashimoto, Toshiyuki Kakudate, Keiichi Katoh, Masahiro Yamashita, Tadahiro Komeda
Summary: The spintronic properties of magnetic molecules have been widely studied, especially in the context of qubits for quantum information processing. This study focuses on the role of the delocalized pi-radical electron spin in the Pc ligand in reading and intermediating the localized Tb spin qubits. Using electron spin resonance (ESR) technique with a scanning tunneling microscope (STM), the researchers were able to measure the local ESR of a single TbPc2 molecule decoupled from the Cu(100) substrate by a two-monolayer NaCl film. The results indicate that the pi-radical electron is delocalized within the ligands and exhibits intramolecular coupling susceptible to the chemical environment.
Review
Chemistry, Multidisciplinary
Christian Werner, Markus Sauer, Christian Geis
Summary: Fluorescence imaging techniques, especially super-resolution microscopy, have significantly improved our understanding of neuronal structure and protein arrangements. This has led to novel insights into neuron function and dysfunction in the past two decades.
Article
Medicine, General & Internal
Max Masthoff, Felix Noah Freppon, Lisa Zondler, Enrica Wilken, Lydia Wachsmuth, Silke Niemann, Christian Schwarz, Ina Fredrich, Asli Havlas, Helena Block, Mirjam Gerwing, Anne Helfen, Walter Heindel, Alexander Zarbock, Moritz Wildgruber, Cornelius Faber
Summary: This study used time-lapse MRI to track the behavior of single circulating monocytes dynamically. It was found that the patrolling behavior of monocytes is influenced by both local and systemic stimuli, and varies depending on the type, duration, and strength of the stimulus.
Article
Multidisciplinary Sciences
Sarah Kono, Aafke van den Berg, Marco Simonetta, Ann Mukhortava, Elspeth F. Garman, Ingrid Tessmer
Summary: AGT is an important DNA repair protein that repairs highly mutagenic and cytotoxic alkylguanine lesions. Studies have revealed the interactions of AGT with alkyl lesions and its role in DNA repair mechanism. Surprisingly, AGT clusters do not enhance DNA translocation speed, suggesting a different role in AGT function.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Patrick Hunter, Alex L. Payne-Dwyer, Michael Shaw, Nathalie Signoret, Mark C. Leake
Summary: The ability of tumors to create a pro-tumorigenic microenvironment is an important area of study in the development of new therapeutics. CCR5, a receptor associated with pro-tumorigenic features, is being considered as a potential target for treatment. Various biophysical methods, such as super-resolution structured illumination microscopy and single molecule partially TIRF-coupled HILO microscopy, can provide valuable insights into the structure and function of onco-immunogenic receptors and other biomolecules.
Article
Chemistry, Physical
Kevin Song, Dmitrii E. Makarov, Etienne Vouga
Summary: Single-molecule and single-particle tracking experiments often cannot accurately measure thermal motion details on short timescales due to continuous trajectories. We demonstrate that the error in measuring the first passage time to a specific domain can be significantly larger than the time resolution when a diffusive trajectory xt is sampled at finite time intervals delta t. This error arises from unobserved trajectory entries and exits, which artificially lengthen the apparent first passage time by more than delta t. These systematic errors are particularly important in single-molecule studies of barrier crossing dynamics. However, we show that the correct first passage times and other trajectory properties can be recovered using a stochastic algorithm that reintroduces unobserved first passage events probabilistically.
JOURNAL OF CHEMICAL PHYSICS
(2023)
Article
Biochemical Research Methods
Longjie Li, Arun Mouli Kolinjivadi, Kok Haur Ong, David Young, Gabriel Pik Liang Marini, Sock Hoai Chan, Siao Ting Chong, Ee Ling Chew, Haoda Lu, Laurent Gole, Weimiao Yu, Joanne Ngeow
Summary: DNA fibre analysis has significant applications in genomic medicine, cancer, and stem cell research. However, currently available software lacks user-friendly interfaces, limiting accuracy and adaptability. To address these limitations, we propose DNA Stranding, a software that offers accurate fibre length quantification and pattern recognition.
Article
Chemistry, Analytical
Xinyu Zhou, Rui Wang, Zijian Wan, Pengfei Zhang, Shaopeng Wang
Summary: In this study, we demonstrate multiplexed protein detection and parallel protein interaction analysis through evanescent scattering microscopy (ESM). ESM allows label-free digital single-molecule counting for binding kinetics measurement, improving counting efficiency and accuracy. We show that digital single-molecule counting can differentiate proteins with different molecular weights, enabling real-time monitoring of protein binding processes in solution. Additionally, we show that this strategy can analyze the kinetics of two different protein interaction processes simultaneously, both on surfaces and in solution. This work may provide a way to investigate complex protein interactions, such as the competition between biomarker-antibody binding in biofluids and biomarker-protein binding on cellular membranes.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Allison R. Cutri, Vignesh Sundaresan, Joshua D. Shrout, Paul W. Bohn
Summary: A device that can capture single Pseudomonas aeruginosa cells and study their spectroelectrochemical behavior has been developed. By capturing cells vertically, three characteristic fluorescence behaviors sensitive to external conditions were observed. This device enables the study of parallel collections of single bacterial cells and facilitates efforts to elucidate methods of bacterial communication and multidrug resistance at the single-cell level.
CELL REPORTS PHYSICAL SCIENCE
(2023)
Article
Chemistry, Analytical
Xinyu Zhou, Rui Wang, Zijian Wan, Pengfei Zhang, Shaopeng Wang
Summary: Multiplexed protein detection and parallel protein interaction analysis are achieved using evanescent scattering microscopy (ESM), enabling label-free digital single-molecule counting with high temporal resolution. This strategy allows for real-time tracking of protein binding processes and simultaneous analysis of multiple protein interactions.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yi Wang, Wenwen Jing, Nongjian Tao, Hui Wang
Summary: The study introduces an optical imaging technique for directly counting and monitoring binding and motion events of single nanoparticles with high sensitivity and temporal resolution, providing a new approach for studying individual dynamic behaviors.
Article
Biology
Shipeng Shao, Hongchen Zhang, Yong Zeng, Yongliang Li, Chaoying Sun, Yujie Sun
Summary: Researchers have developed the TagBiFC technique based on split HaloTag for visualizing protein-protein interactions in living cells, showing better brightness and photostability compared to fluorescent proteins. The reconstituted HaloTag exhibited higher localization precision and longer tracking length, revealing unique interactions of transcription factor dimers with chromatin DNA and demonstrating advantageous applications in various imaging techniques. The superior properties of TagBiFC system are believed to have broad applications in single molecule imaging studies inside living cells.
COMMUNICATIONS BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Nitesh Kumar Podh, Sheetal Paliwal, Partha Dey, Ayan Das, Shruti Morjaria, Gunjan Mehta
Summary: Single-molecule imaging in live yeast cells has become a powerful tool for researchers to quantitatively measure cellular activities in real time, with significant progress made in recent years to provide important references for studying biological processes. Different labs have tailored imaging setups and data analysis pipelines to estimate biophysical parameters for various biological processes, reflecting a rich variety of experimental experiences.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Review
Chemistry, Physical
Wei Xu, Ruihao Li, Chenhao Wang, Jiahe Zhong, Junyang Liu, Wenjing Hong
Summary: This review article explores the excitation mechanisms between molecules and plasmonic nanocavities, discusses the influence of nanostructures on excited-state properties, and presents potential applications and challenges in single-molecule optoelectronic devices.
Article
Biochemical Research Methods
Charles J. Richardson, Eric A. First
ANALYTICAL BIOCHEMISTRY
(2015)
Article
Microbiology
Sara A. Leiman, Charles Richardson, Lucy Foulston, Alexander K. W. Elsholz, Eric A. First, Richard Losick
JOURNAL OF BACTERIOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Charles J. Richardson, Eric A. First
Article
Biochemistry & Molecular Biology
Charles J. Richardson, Eric A. First
Article
Biochemical Research Methods
Eric A. First, Charles J. Richardson
Article
Cell Biology
Julian Michael Rocha, Charles Joseph Richardson, Mingxing Zhang, Caroline Maureen Darch, Eugene Cai, Andreas Diepold, Andreas Gahlmann
INTEGRATIVE BIOLOGY
(2018)
Article
Oncology
Gulshan Sunavala-Dossabhoy, Senthilnathan Palaniyandi, Charles Richardson, Arrigo De Benedetti, Lisa Schrott, Gloria Caldito
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2012)
Article
Cell Biology
Jennifer R. Larson, Jennifer P. Bharucha, Shantelle Ceaser, Joanna Salamon, Charles J. Richardson, Segalit M. Rivera, Kelly Tatchell
MOLECULAR BIOLOGY OF THE CELL
(2008)
Article
Biochemistry & Molecular Biology
Eduard Nedea, Demet Nalbant, Daniel Xia, Nathaniel T. Theoharis, Bernhard Suter, Charles J. Richardson, Kelly Tatchel, Thomas Kislinger, Jack F. Greenblatt, Peter L. Nagy
Article
Optics
T. Yan, C. J. Richardson, M. Zhang, A. Gahlmann
Article
Multidisciplinary Sciences
Charles J. Richardson, Eric A. First