Review
Biochemical Research Methods
Ramon Lorenzo-Gomez, Rebeca Miranda-Castro, Noemi De-los-Santos-alvarez, Maria Jesus Lobo-Castanon
Summary: The role of extracellular matrix (ECM) in tumorigenesis and metastasis is crucial, as tumor cells need to recruit a permissive microenvironment for growth. The interaction between tumor cells and ECM leads to the release of ECM-derived molecules into the bloodstream, which can serve as potential biomarkers for tumor development. Developing better bioanalytical methods for detecting ECM remodeling-derived components is essential for translating basic knowledge about cancer progression into clinical practice.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Oncology
Barbara Cardinali, Roberta Tasso, Patrizia Piccioli, Maria Chiara Ciferri, Rodolfo Quarto, Lucia Del Mastro
Summary: This review discusses the potential application of circulating microRNAs (miRNAs) as diagnostic and monitoring biomarkers in breast cancer. It highlights the advantages and challenges that need to be addressed. Although further studies are needed to support their clinical utility, miRNAs could represent novel tools for breast cancer management.
Article
Gastroenterology & Hepatology
Stine Johansen, Mads F. Israelsen, Ida Villesen, Nikolaj J. Torp, Mette Nielsen, Maria P. Kjaergaard, Katrine D. Lindvig, Camilla Hansen, Peter N. Andersen, Ditlev Rasmussen, Sonke J. Detlefsen, Diana Leeming, Maja Thiele, Morten Karsdal, Aleksander Krag, Ema Anastasiadou, Manimozhian Arumugam, Peer Bork, Torben Hansen, Roland Henrar, Hans Israelsen, Morten Karsdal, Cristina Legido-Quigley, Hans Olav Melberg, Maja Thiele, Jonel Trebicka, Aleksander Krag, Mathias Mann, Jelle Matthijnssens
Summary: This study aimed to assess the PRO-C3 model in predicting liver-related events in patients with alcohol-related liver disease. The results showed that the PRO-C3 model had high prognostic accuracy in predicting these events.
LIVER INTERNATIONAL
(2023)
Review
Biochemistry & Molecular Biology
Natalia Georgantzoglou, Alexandros Pergaris, Christos Masaoutis, Stamatios Theocharis
Summary: Exosomes, extracellular vesicles containing biomolecular cargo, play a crucial role in intercellular communication and oncogenesis. Urine is suggested as a rich source of exosomal biomarkers with potential applications in bladder cancer management. Further studies optimizing sample collection procedures and analytical methods are needed to enhance the diagnostic and therapeutic potential of exosomal biomarkers in bladder cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Codrin-Constantin Burlacu, Daniela Ciobanu, Andrei-Vlad Badulescu, Vlad-Florin Chelaru, Andrei-Otto Mitre, Bogdan Capitanescu, Dirk M. Hermann, Aurel Popa-Wagner
Summary: Stroke is the second leading cause of death and disability, with limited therapeutic strategies. Blood-based biomarkers, such as microRNAs and exosomes, are recognized as useful tools to predict prognosis in patients with stroke. This systematic review identified 72 circulating biomarkers with prognostic value in stroke recovery, including 68 miRNAs predictive for motor recovery and seven biomarkers predictive for cognitive recovery. The most significant correlations were found with miR-21, miR-29b, miR-125b-5p, miR-126, and miR-335. Serum miR-9 and neutrophil miR-29b were identified as promising biomarkers for further studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Claudia Daniela Alvarez-Zuniga, Idalia Garza-Veloz, Jacqueline Martinez-Rendon, Misael Ureno-Segura, Ivan Delgado-Enciso, Margarita L. Martinez-Fierro
Summary: Acute lymphoblastic leukemia (ALL) is a hematological disease characterized by dysfunction of the hematopoietic system, leading to arrest in stem cell development and reduced production of blood components. BCP-ALL is a neoplasm that arises from B-cell progenitor lineage and is driven by various mechanisms with tumor potential. Finding noninvasive and accessible circulating biomarkers for diagnosis, prognosis, and disease monitoring is crucial. Evaluation: 9/10.
Review
Oncology
Sergio Antonio Oropeza-de Lara, Idalia Garza-Veloz, Bertha Berthaud-Gonzalez, Margarita L. Martinez-Fierro
Summary: In developed countries, endometrial cancer is a common neoplasm of the female reproductive system. MicroRNAs, single-stranded noncoding RNA molecules, have been studied for their diagnostic, prognostic, and treatment response potential in EC. However, the heterogeneity in research methodologies, sample types, selection criteria, and results makes it challenging to identify potential miR biomarkers. This study aimed to summarize the current knowledge on miRs that have shown the most suitability as potential markers for EC.
Article
Oncology
Troels D. Christensen, Christina Jensen, Ole Larsen, Bonna Leerhoy, Carsten P. Hansen, Kasper Madsen, Dan Hogdall, Morten A. Karsdal, Inna M. Chen, Dorte Nielsen, Julia S. Johansen, Nicholas Willumsen
Summary: Collagen remodeling is increased in patients with biliary tract cancer (BTC) and is associated with survival. The pro-peptides of collagen (PRO-C3 and PRO-C6) could be used as novel biomarkers for these patients.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Cell Biology
Miriana d'Alessandro, Piera Soccio, Laura Bergantini, Paolo Cameli, Giulia Scioscia, Maria Pia Foschino Barbaro, Donato Lacedonia, Elena Bargagli
Summary: This multicenter study revealed significant differences in exosomal surface epitope expression between IPF patients and healthy controls. Higher levels of markers such as CD19, CD69, CD8, and CD86 were observed in IPF patients, while CD42a was lower. Survival analysis indicated potential predictive value of CD25 and CD8 expression levels in IPF patient outcomes.
Review
Biochemistry & Molecular Biology
Lauren A. Newman, Kate Muller, Andrew Rowland
Summary: This study summarizes the contribution of extracellular vesicles (EVs) to normal liver function and chronic liver diseases. It explores the use of circulating EV biomarkers for monitoring disease status and treatment response. Emerging technologies for selectively isolating and analyzing circulating EVs derived only from hepatic cells show promise for biomarker platforms in chronic liver diseases.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Sarah Ali-Berrada, Jeanne Guitton, Sophie Tan-Chen, Anna Gyulkhandanyan, Eric Hajduch, Herve Le Stunff
Summary: Sphingolipids are lipid molecules produced in mammals and play important roles in cellular processes and glucose metabolism. Ceramides and sphingosine-1-phosphate (S1P) are two sphingolipid species abundantly found in the bloodstream and associated with lipoproteins or extracellular vesicles. The concentrations of these sphingolipids in plasma can be altered in metabolic disorders and may serve as predictive biomarkers. Advances in understanding the molecular mechanisms of ceramides and S1P association with lipoproteins or extracellular vesicles and their impact on glucose metabolism are discussed in this review.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Respiratory System
Nils Hoyer, Henrik Jessen, Thomas S. Prior, Jannie M. B. Sand, Diana J. Leeming, Morten A. Karsdal, Emilia K. A. Attingsberg, Gustav K. M. Vangsgaard, Elisabeth Bendstrup, Saher B. Shaker
Summary: Blood biomarkers of types III and VI collagen turnover measured at the time of diagnosis are associated with disease severity, short-term progression, and long-term mortality in patients with idiopathic pulmonary fibrosis (IPF). High baseline levels of these biomarkers may indicate more advanced disease and higher risk of progression or death.
Article
Reproductive Biology
Emma Gerber, Meshach Asare-Werehene, Arkadiy Reunov, Dylan Burger, Tien Le, Euridice Carmona, Anne-Marie Mes-Masson, Benjamin K. K. Tsang
Summary: Resistance to chemotherapy in epithelial ovarian cancer (EOC) remains a challenge, and there is a need for predictive biomarkers. This study found that circulating small extracellular vesicle (sEV)-plasma gelsolin (pGSN) could be a predictive biomarker for chemoresistance in EOC, outperforming conventional biomarkers such as CA125.
JOURNAL OF OVARIAN RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Dongchan Kim, Bhavya Gupta, Geoffrey Yuet Mun Wong
Summary: The liver is the most common site of metastasis in colorectal cancer. Multimodal treatment, including liver resection, is potentially curative and prolongs survival for selected patients with colorectal liver metastases (CRLM). However, the treatment of CRLM remains challenging because recurrence is common, and prognosis varies widely between patients despite curative-intent treatment. Clinicopathological features and tissue-based molecular biomarkers, either alone or in combination, are insufficient for accurate prognostication. As most of the functional information in cells resides in the proteome, circulating proteomic biomarkers may be useful for rationalising the molecular complexities of CRLM and identifying potentially prognostic molecular subtypes. High-throughput proteomics has accelerated a range of applications including protein profiling of liquid biopsies for biomarker discovery. Moreover, these proteomic biomarkers may provide non-invasive prognostic information even before CRLM resection. This review evaluates recently discovered circulating proteomic biomarkers in CRLM. We also highlight some of the challenges and opportunities with translating these discoveries into clinical applications. (c) 2023 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Arman Sourani, Saeid Saghaei, Masih Sabouri, Masoud Soleimani, Leila Dehghani
Summary: This systematic review explored the potential application of Extracellular vesicles (EVs) in the diagnosis, prognosis, and treatment response monitoring of gliomas. The majority of studies suggested that EVs, particularly with different types of miRNAs, hold considerable potential for diagnostics. However, limited evidence regarding prognosis and treatment response monitoring inhibits drawing practical conclusions about EVs.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Multidisciplinary Sciences
Andrea Cairo, Marilena Iorio, Silvia Spena, Elda Tagliabue, Flora Peyvandi
Summary: This study analyzed the genetic diversity and distribution of SARS-CoV-2 in the early stages of the COVID-19 pandemic. The researchers identified thousands of genetic variants, clustering into four major clades with specific geographical distributions. The most common variant, p.Asp614Gly, was found to occur in conjunction with three other variants, suggesting a high linkage. Additionally, the study observed a synergistic effect in the mutations characterizing the British variant.
Review
Oncology
Martina Di Modica, Valeria Arlotta, Lucia Sfondrini, Elda Tagliabue, Tiziana Triulzi
Summary: Microbiota plays a key role in cancer by influencing various host physiological functions and affecting the efficacy of anticancer treatments. The involvement of the breast microbiota in breast cancer has been extensively studied. This review focuses on the impact of gut and breast microbiota on HER2+ breast cancer subtype and discusses the potential of defining microbial signatures associated with disease aggressiveness, treatment response, and therapy toxicity. The mechanisms of microorganism-host interactions and the possibility of microbiota editing in breast cancer prevention and treatment optimization are also explored.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Milena Perrone, Claudia Chiodoni, Mara Lecchi, Laura Botti, Barbara Bassani, Annamaria Piva, Elena Jachetti, Matteo Milani, Daniele Lecis, Elda Tagliabue, Paolo Verderio, Sabina Sangaletti, Mario P. Colombo
Summary: Cancer can reprogram the bone marrow niche through the BM mesenchymal stem cells (MSC) and IL-1B/ATF3 signaling pathway, which promotes myeloid cell differentiation and tumor development. The expression of CD11b thorn population in the peripheral blood can be used as a potential biomarker for early diagnosis of breast cancer. This study suggests that targeting IL-1B or ATF3 may have therapeutic benefits for breast cancer patients.
Article
Biochemistry & Molecular Biology
Valentino Le Noci, Giancarla Bernardo, Giacomo Manenti, Gabriele Infante, Dariush Khaleghi Hashemian, Lucia Minoli, Simone Canesi, Francesca Bianchi, Tiziana Triulzi, Stefania Arioli, Loris De Cecco, Simone Guglielmetti, Federico Ambrogi, Camilla Recordati, Nicoletta Gagliano, Elda Tagliabue, Michele Sommariva, Lucia Sfondrini
Summary: This study explores the impact of perturbing lung microbiota on lung cancer development by using antibiotics or probiotic aerosol. The results suggest that L.RGG aerosol can reduce tumor nodules and Tregs in the lung, while increasing the expression of immunoglobulin joining chain and promoting B and CD4 T cell infiltration. This non-invasive strategy shows efficacy in impairing lung cancer growth by promoting local immunity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Valentina Fogazzi, Marcel Kapahnke, Alessandra Cataldo, Ilaria Plantamura, Elda Tagliabue, Serena Di Cosimo, Giulia Cosentino, Marilena V. Iorio
Summary: Breast cancer is the most commonly diagnosed malignancy in women globally, with 20% of cases being HER2 positive. Despite the improvement in prognosis with anti-HER2 agents, a significant number of patients do not respond to treatment. MicroRNAs are promising clinical tools for managing HER2 positive breast cancers due to their detectability and stability in tissues and blood, as well as their potential as therapeutic agents, prognostic and predictive biomarkers.
Article
Oncology
Giancarla Bernardo, Valentino Le Noci, Emerenziana Ottaviano, Loris De Cecco, Chiara Camisaschi, Simone Guglielmetti, Martina Di Modica, Giorgio Gargari, Francesca Bianchi, Serena Indino, Patrizia Sartori, Elisa Borghi, Michele Sommariva, Elda Tagliabue, Tiziana Triulzi, Lucia Sfondrini
Summary: This study highlights the significant influence of mammary tumor microbiota on local immune status and the relevance of its treatment with antibiotics, in combination with breast cancer therapies.
Article
Biochemistry & Molecular Biology
Serena Indino, Cristina Borzi, Claudia Moscheni, Patrizia Sartori, Loris De Cecco, Giancarla Bernardo, Valentino Le Noci, Francesca Arnaboldi, Tiziana Triulzi, Gabriella Sozzi, Elda Tagliabue, Lucia Sfondrini, Nicoletta Gagliano, Massimo Moro, Michele Sommariva
Summary: Certain malignant cells may induce an HPD-related phenotype in macrophages, accelerating tumor growth, while tumor-derived Extracellular Vesicles play a key role in macrophage re-education program.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Tiziana Triulzi, Viola Regondi, Elisabetta Venturelli, Patrizia Gasparini, Cristina Ghirelli, Jessica Groppelli, Martina Di Modica, Francesca Bianchi, Loris De Cecco, Lucia Sfondrini, Elda Tagliabue
Summary: HER2 mRNA expression is associated with the response to anti-HER2 treatment and is independent of estrogen receptor tumor status in HER2-positive breast cancer patients. HER2 mRNA levels can predict the risk and exhibit distinct biological characteristics compared to HER2 protein levels.
Article
Oncology
G. Lo Russo, F. Sgambelluri, A. Prelaj, F. Galli, S. Manglaviti, A. Bottiglieri, R. M. Di Mauro, R. Ferrara, G. Galli, D. Signorelli, A. De Toma, M. Occhipinti, M. Brambilla, E. Rulli, T. Triulzi, T. Torelli, L. Agnelli, S. Brich, A. Martinetti, A. D. Dumitrascu, V Torri, G. Pruneri, A. Fabbri, F. de Braud, A. Anichini, C. Proto, M. Ganzinelli, R. Mortarini, M. C. Garassino
Summary: The study found that circulating immune biomarkers can predict outcomes in negative and low PD-L1 aNSCLC patients treated with first-line pembrolizumab. The results showed that higher T cell and NK cell counts were associated with improved survival and disease control, while higher myeloid cell counts were associated with worse survival and disease control.
Article
Oncology
Arsela Prelaj, Monica Ganzinelli, Francesco Trovo, LailaC. Roisman, Alessandra Laura Giulia Pedrocchi, Sokol Kosta, Marcello Restelli, Emilia Ambrosini, Massimo Broggini, Gabriella Pravettoni, Dario Monzani, Alessandro Nuara, Ramon Amat, Nikos Spathas, Michael Willis, Alexander Pearson, James Dolezal, Laura Mazzeo, Sabina Sangaletti, Ana Maria Correa, Alfonso Aguaron, Iris Watermann, Crina Popa, Giulia Raimondi, Tiziana Triulzi, Stefan Steurer, Giuseppe Lo Russo, Helena Linardou, Nir Peled, Enriqueta Felip, Martin Reck, Marina Chiara Garassino
Summary: Despite the success of immunotherapy in treating aNSCLC, only a fraction of patients truly benefit from it. PD-L1, the only biomarker used for predicting IO outcomes, is not sufficient due to the complex nature of the immune system and tumor microenvironment. Artificial Intelligence and Machine Learning are now utilized to develop decision-making tools to match treatments with individual patients, improve outcomes, and reduce the economic burden. I3LUNG, a funded study, aims to promote individualized treatment in aNSCLC through AI-based tools and create an integrated platform for IO administration.
CLINICAL LUNG CANCER
(2023)
Review
Cell Biology
Giancarla Bernardo, Valentino Le Noci, Martina Di Modica, Elena Montanari, Tiziana Triulzi, Serenella M. Pupa, Elda Tagliabue, Michele Sommariva, Lucia Sfondrini
Summary: Emerging evidence suggests that the composition of the gut microbiota is closely associated with breast cancer progression. In addition to their role in modulating the immune response and estrogen levels, recent research has revealed the presence of microbes in the breast tissue, which was once considered sterile, and their potential contribution to tumor progression. Specific bacterial species enriched in breast tumors have been identified, and their mechanisms of influencing cancer progression have been elucidated. Understanding the crosstalk between the gut/mammary microbiota and breast cancer holds promise for developing innovative therapeutic approaches.
