Review
Biochemistry & Molecular Biology
Yi-Ping Yang, Yu-Jer Hsiao, Kao-Jung Chang, Shania Foustine, Yu-Ling Ko, Yi-Ching Tsai, Hsiao-Yun Tai, Yu-Chieh Ko, Shih-Hwa Chiou, Tai-Chi Lin, Shih-Jen Chen, Yueh Chien, De-Kuang Hwang
Summary: Human pluripotent stem cells (PSCs) have the potential to replace diseased or injured tissues and are widely used in regenerative medicine. Induced pluripotent stem cells (iPSCs) can differentiate into various cell types and are being explored for the treatment of retinal diseases. Further clinical trials are needed to assess the efficacy and adverse effects of iPSC-based therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell & Tissue Engineering
Azam Shamsian, Roxana Sahebnasagh, Amir Norouzy, Safin Hassan Hussein, Mohammad Hossein Ghahremani, Zahra Azizi
Summary: Induced pluripotent stem cells (iPSCs) have wide-ranging potential applications in medical research, and cancer cells may serve as a new source for generating iPSCs. Although cancer cells share similarities with iPSCs, they carry genetic abnormalities and pose a risk of tumor formation. This review discusses the feasibility of using cancer cells as a preferred source for iPSC generation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Naresh Polisetti, Julian Rapp, Paula Liang, Viviane Dettmer-Monaco, Felicitas Bucher, Jan Pruszak, Ursula Schloetzer-Schrehardt, Toni Cathomen, Guenther Schlunck, Thomas Reinhard
Summary: hiPSC-LEPC and T-LEPC exhibit similar gene expression patterns, colony-forming ability, wound-healing capacity, and melanosome uptake, suggesting that hiPSC-LEPC could be a potential cell source for the treatment of LSCD.
Article
Biochemistry & Molecular Biology
Jieun Baek, Bokyeong Ryu, Jin Kim, Seul-Gi Lee, Min-Seok Oh, Ki-Sung Hong, Eun-Young Kim, C-Yoon Kim, Hyung-Min Chung
Summary: This study compared M-MSCs with adult-MSCs and found that M-MSCs have the potential to be used in next-generation therapies for rotator cuff tears (RCTs). They are able to regulate inflammation and promote tissue repair, leading to improved muscle recovery in RCTs.
Review
Pharmacology & Pharmacy
Lu Zhang, Xiao-Jing-Nan Ma, Yuan-Yuan Fei, Heng-Tong Han, Jun Xu, Lu Cheng, Xun Li
Summary: The liver has the ability to repair itself, but pathological changes can impair its regenerative capacity and lead to liver failure. Mesenchymal stem cells (MSCs) have shown promise in liver regeneration, and induced pluripotent stem cells have made progress in organoid establishment and drug screening.
PHARMACOLOGY & THERAPEUTICS
(2022)
Review
Cell & Tissue Engineering
Victoria Dupuis, Elisa Oltra
Summary: Mesenchymal stem cells (MSCs) have great potential for cell therapy due to their immunomodulatory effects, but their clinical application is restricted by several factors. Induced pluripotent stem cells (iPSCs) offer a new source to generate MSCs, known as iMSCs, which may overcome technical and biological limitations and ethical concerns regarding human embryonic stem cells. Various differentiation methods for iPSCs into MSCs have been developed to meet clinical demands.
WORLD JOURNAL OF STEM CELLS
(2021)
Review
Biotechnology & Applied Microbiology
Wei Cheng, Chengming Fan, Qing Song, Ping Chen, Hong Peng, Ling Lin, Cong Liu, Bin Wang, Zijing Zhou
Summary: Fibrotic diseases are responsible for a third of global deaths, leading to organ remodeling and dysfunction. Despite the complications of organ transplantation, induced pluripotent stem cell (iPSC)-based therapy shows potential as a treatment option by replacing fibrotic organs. Recent studies have successfully differentiated iPSCs into fibrosis-relevant cell types with anti-fibrotic effects, providing promising precision treatments for organ-specific fibrosis. This review summarizes the potential, advantages, challenges, and future outlook of iPSC-based approaches in treating organ fibrosis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Immunology
Ratchapong Netsrithong, Methichit Wattanapanitch
Summary: This review discusses the current status and future directions of CAR T cell therapy, exploring the potential of using iPSCs as a source of T cells and the genome engineering techniques for generating next-generation CAR T cells. It also highlights the challenges faced at present and the prospects for future universal off-the-shelf immunotherapeutic products.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Ying Zhang, Jun Wei, Jiani Cao, Kehua Zhang, Yaojin Peng, Hongkui Deng, Jiuhong Kang, Guangjin Pan, Yong Zhang, Boqiang Fu, Shijun Hu, Jie Na, Yan Liu, Lei Wang, Lingmin Liang, Huanxin Zhu, Yu Zhang, Zi-Bing Jin, Jie Hao, Aijin Ma, Tongbiao Zhao, Junying Yu
Summary: "Requirements for Human-Induced Pluripotent Stem Cells" is the first set of guidelines in China on human-induced pluripotent stem cells. It provides detailed technical requirements and aims to promote international standardization.
CELL PROLIFERATION
(2022)
Review
Biochemistry & Molecular Biology
Li-Jie Hsu, Chao-Lin Liu, Ming-Ling Kuo, Chia-Ning Shen, Chia-Rui Shen
Summary: Cell therapy involves using cells selected, manipulated, and treated outside the body to treat or prevent human disease. iPSCs are ideal cell sources due to their unique characteristics. NK cells are effective tumor killers without causing GVHD, making them excellent candidates for cell therapy.
Article
Biochemistry & Molecular Biology
Xuehui Fan, Lukas Cyganek, Katja Nitschke, Stefanie Uhlig, Philipp Nuhn, Karen Bieback, Daniel Duerschmied, Ibrahim El-Battrawy, Xiaobo Zhou, Ibrahim Akin
Summary: This study compared the electrophysiological and functional properties of endothelial cells derived from human induced pluripotent stem cells with primary human cardiac microvascular endothelial cells. The results demonstrated that the two types of endothelial cells were similar in terms of ion channel function, membrane receptor signaling, and physiological cell functions, although some differences were observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Ying Chen, Qing Yin, Xiao-Yu Cheng, Jin-Ru Zhang, Hong Jin, Kai Li, Cheng-Jie Mao, Fen Wang, Hong-Zhe Bei, Chun-Feng Liu
Summary: Induced pluripotent stem cells (iPSCs) can successfully mimic age-related diseases, such as Parkinson's disease (PD). In this study, we differentiated dopaminergic (DA) neurons from iPSCs of PD patients with the G2019S LRRK2 mutation and found morphological changes compared to control group. Furthermore, we observed that these mutated DA neurons were more vulnerable to damage, with higher rates of apoptosis and inflammatory response.
FRONTIERS IN NEUROSCIENCE
(2022)
Review
Oncology
Yang Zhou, Miao Li, Kuangyi Zhou, James Brown, Tasha Tsao, Xinjian Cen, Tiffany Husman, Aarushi Bajpai, Zachary Spencer Dunn, Lili Yang
Summary: Cell-based immunotherapy has revolutionized the treatment of hematological malignancies, but obstacles remain in the widespread clinical applications of current autologous therapies. Human induced pluripotent stem cells offer a potential solution by providing a scalable source for cell therapy, and recent advances have been made in using these cells for immune therapy, particularly in combination with CAR-engineering.
Article
Cardiac & Cardiovascular Systems
Samantha K. Atkins, Abhijeet R. Sonawane, Romi Brouwhuis, Johana Barrientos, Anna Ha, Maximillian Rogers, Takeshi Tanaka, Takehito Okui, Shiori Kuraoka, Sasha A. Singh, Masanori Aikawa, Elena Aikawa
Summary: Cardiovascular calcification is a major predictor of cardiovascular events and a leading cause of global morbidity and mortality. This study presents a model of induced pluripotent stem cell-derived smooth muscle cells (iSMCs) for studying the pathogenesis of vascular calcification. The study successfully differentiated iPSCs into iSMCs and induced calcification in the iSMCs, providing an in vitro model for future research on cardiovascular calcification.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Nicole Pui-Yu Ho, Hitoshi Takizawa
Summary: This article summarizes the current understanding of how hematopoietic stem cell functions are maintained, damaged, or exhausted during acute, prolonged, and pathological inflammatory conditions. It also highlights the impact of inflammation-altered hematopoietic stem cell niche on escalating the insults on hematopoietic stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Ghmkin Hassan, Toshiaki Ohara, Said M. Afify, Kazuki Kumon, Maram H. Zahra, Xiaoying Fu, Mohamad Al Kadi, Akimasa Seno, David S. Salomon, Masaharu Seno
Summary: This study found that normal stem cells can be converted into cancer stem cells in the cancer-inducing microenvironment. The converted cells exhibited tumorigenicity and self-renewal characteristics and their drug responses and metastatic abilities varied depending on the microenvironment of cancer cells. Transcriptome analysis showed upregulation of pathways related to aggressiveness and energy production in the converted cells.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Multidisciplinary Sciences
Yanning Xu, Said M. Afify, Juan Du, Bingbing Liu, Ghmkin Hassan, Qing Wang, Hanbo Li, Yixin Liu, Xiaoying Fu, Zhengmao Zhu, Ling Chen, Masaharu Seno
Summary: This study established a model of cancer stem cells (CSCs) derived from mouse induced pluripotent stem cells (miPSCs) and found that the activation of PI3K-Akt and EGFR signaling pathways played significant roles in the process. The inhibitors targeting these pathways showed significant anti-tumor effects in vitro and in vivo, with PI3K-gamma inhibitor Eganelisib demonstrating better therapeutic efficacy and fewer side effects.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Said M. Afify, Ghmkin Hassan, Akimasa Seno, Masaharu Seno
Summary: The growth of cancer tissue is driven by a small group of cells called cancer stem cells, which have the potential to self-renew, differentiate, and form tumors. Chronic diseases like obesity and diabetes may establish an environment that promotes the transformation of undifferentiated cells into cancer stem cells and the development of malignant tumors. Chronic inflammation is believed to play a key role in this process.
BRITISH JOURNAL OF CANCER
(2022)
Article
Oncology
Ling Chen, Yanxia Liu, Yanning Xu, Said M. Afify, Ang Gao, Juan Du, Bingbing Liu, Xiaoying Fu, Yixin Liu, Ting Yan, Zhengmao Zhu, Masaharu Seno
Summary: In this study, it was discovered that the up-regulation of Dsg2 expression during the conversion of miPSCs into CSCs activates the Wnt/beta-catenin signaling pathway, implying a potential mechanism for the transformation of stem cells into a malignant phenotype.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Ghmkin Hassan, Said M. Afify, Maram H. Zahra, Hend M. Nawara, Kazuki Kumon, Yoshiaki Iwasaki, David S. Salomon, Akimasa Seno, Masaharu Seno
Summary: Researchers have successfully converted human induced pluripotent stem cells into cancer stem cells by culturing them in a medium mimicking the microenvironment of tumor initiation. The converted cells exhibited characteristics of cancer stem cells and could be used to establish personalized cancer models for studying tumor initiation and screening personalized therapies.
Article
Engineering, Biomedical
Said M. Afify, Ghmkin Hassan, Maram H. Zahra, Hend M. Nawara, Hagar A. Abu Quora, Amira Osman, Hager Mansour, Kazuki Kumon, Akimasa Seno, Ling Chen, Ayano Satoh, David S. Salomon, Masaharu Seno
Summary: Tumor associated myoepithelial cells (TAMEs) are a crucial population in the tumor microenvironment, originating from cancer stem cells (CSCs) and playing a key role in tumor development.
Meeting Abstract
Oncology
Hiroko Ishii, Maram Zahra, Ghmkin Hassan, Masaharu Seno
Meeting Abstract
Oncology
Monzur Sadia, Ghmkin Hassan, Said M. Affify, Hager Mansour, Mona Sheta, Kazuki Kumon, Maram H. Zahra, Akimasa Seno, Masaharu Seno
Meeting Abstract
Oncology
Akimasa Seno, Maram Zahra, Masaharu Seno
Meeting Abstract
Oncology
Hassan Ghmkin, Said M. Afify, Maram H. Zahara, David S. Salomon, Akimasa Seno, Masaharu Seno