Journal
ACS SENSORS
Volume 4, Issue 4, Pages 961-967Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.9b00063
Keywords
pathogen; gold; nanoparticle; colorimetric; LSPR; sepsis; urine; magnetic
Funding
- [CTQ2017-92226-EXP]
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Identifying the pathogen responsible for an infection is a requirement in order to personalize antimicrobial treatments. Detecting bacterial enzymes, such as proteases, lipases, and oxidoreductases, is a winning approach for detecting pathogens at the point of care. In this Article, a new method for detecting urease-producing bacteria rapidly and at ultralow concentrations is reported. In this method, longsome bacteriological culture steps are substituted for a 10 min capture procedure with positively charged magnetic beads. The presence of urease-positive bacteria on the particles is then queried with a plasmonic signal generation step that generates blue- or red-colored nanoparticle suspensions upon addition of the enzyme substrate. These colorimetric signals, which can be easily identified by eye, are generated by the NH3-dependent assembly of gold nanoparticles in the presence of bovine serum albumin (BSA). The proposed method can detect Proteus mirabilis with a limit of detection of 10(1) cells mL(-1), with a total assay time of 40 min, even in the presence of a large excess of urease-negative bacteria (Pseudomonas aeruginosa). Furthermore, it does not require bulky equipment, and it can detect P. mirabilis at clinically relevant concentrations within minutes, making it suitable for detecting urease-positive pathogens at the point of care.
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