Article
Biophysics
Naveen K. Singh, Partha Ray, Aaron F. Carlin, Celestine Magallanes, Sydney C. Morgan, Louise C. Laurent, Eliah S. Aronoff-Spencer, Drew A. Hall
Summary: The study introduces an aptamer-based SARS-CoV-2 salivary antigen assay that utilizes low-cost reagents and an off-the-shelf glucometer. This test is highly scalable, provides rapid results within 1 hour, and can accurately detect viral loads in infected specimens while distinguishing them from uninfected controls.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Shan Jiang, Lvxia Zhang, Jizhou Li, Hui Ouyang, Zhifeng Fu
Summary: A sensitive immunochromatographic test strip (ITS) for detecting AFB(1) using dendritic platinum nanoparticles as pressure/colorimetric dual-readout probes was developed. The ITS showed a detection range of 0.05-10 ng mL(-1) with a detection limit of 0.03 ng mL(-1) for AFB(1). It was successfully utilized for detecting AFB(1) in herbal medicine samples, demonstrating its reliability for real sample detection and potential applications in drug and food quality control, pollutants monitoring, and medical diagnosis.
Article
Chemistry, Analytical
Chung-Hsuan Yang, Ching-Hsiu Tsai
Summary: Aflatoxin B1 (AFB1) is a common mycotoxin that poses a threat to food and feed safety. In this study, we generated AFB1-specific aptamers using SELEX technology and developed an aptasensor based on AFB1. The aptasensor showed a broad range of detection for AFB1 in peanut extract samples, with high accuracy.
Article
Nanoscience & Nanotechnology
Dai Lu, Hao Jiang, Guangyin Zhang, Qian Luo, Qian Zhao, Xingbo Shi
Summary: This study developed a novel multimode NLISA for ultrasensitive detection of AFB1 using in situ generated PBNPs. Photothermal and colorimetric signals allow portable/visual point-of-care testing, while fluorescent signals enable accurate determination.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Applied
Supan Cheng, Mashooq Khan, Fangchao Yin, Chunxia Ma, Jinpeng Yuan, Ting Jiang, Xuefeng Liu, Qiongzheng Hu
Summary: In this study, a liquid-crystal (LC) based assay for rapid detection of AFB1 in food samples was designed. The LC-based sensing platform provides simple operation, remarkable sensitivity, high selectivity, low cost, and excellent portability without the use of any bulky instrument, which is very promising in rapid on-field detection of mycotoxins.
Article
Biochemistry & Molecular Biology
Chao Wang, Hao Yu, Qiang Zhao
Summary: In this study, a aptamer-based method using structure-switch for fluorescent detection of aflatoxin B1 (AFB1) was reported. The method allowed detection of AFB1 in complex sample matrix with high detection limit and dynamic concentration range.
Article
Biophysics
Yuye Li, Dong Liu, Shuyun Meng, Ting Chen, Chang Liu, Tianyan You
Summary: This study presents a dual-ratiometric electrochemical aptasensor for the detection of AFB1, integrating two aptamers with varied affinity to expand the dynamic range. The sensor allows for rapid positive/negative detection of AFB1 with a threshold concentration of 10 pg mL-1, and offers two linear ranges for quantitative detection.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Applied
Junning Ma, Yue Guan, Fuguo Xing, Yan Wang, Xu Li, Qiang Yu, Xiaohua Yu
Summary: To detect aflatoxin B1 (AFB1) in foodstuffs on-site, we developed a smartphone-based chemiluminescence detection method using both labelled and label-free modes. The labelled mode achieved a detection limit of 0.04 ng/mL and a linear range of 1-100 ng/mL through double streptavidin-biotin mediated signal amplification. To simplify the system, a label-free mode was fabricated using split aptamer and split DNAzyme, which achieved a satisfactory detection limit of 0.33 ng/mL in the same linear range. Both systems showed excellent recovery rates in AFB1-spiked maize and peanut kernel samples. The integration of these systems into a smartphone-based portable device allowed for comparable AFB1 detection ability to a commercial microplate reader. These systems have great potential for on-site AFB1 detection in the food supply chain.
Review
Food Science & Technology
Xiaodong Guo, Mengzhi Wang
Summary: This article discusses the recent progress in optical and electrochemical aptasensors for monitoring AFB1 over the past three years. It highlights the use of advanced nanomaterials for improved analytical performance and provides a critical analysis of various signal transduction strategies for aptasensor construction. Furthermore, it reveals the challenges and future opportunities for the development of aptasensors.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Chemistry, Analytical
Chao Wang, Kexiao Zhu, Jie Yu, Pengfei Shi
Summary: This paper presents an improved molecular beacon method for the rapid and sensitive detection of aflatoxin B1 (AFB1), a highly carcinogenic natural mycotoxin. The molecular beacon, consisting of a DNA aptamer flanked by FAM and BHQ1, showed a larger fluorescent response to AFB1 with the assistance of complementary DNA (cDNA) chain. By optimizing key experimental factors, the method achieved rapid detection of AFB1 within 20 minutes in a concentration range of 1 nM to 3 μM. The aptamer-based molecular beacon method demonstrated easy operation, rapid analysis, larger signal response, good specificity, anti-interference ability, and potential for real-sample analysis.
Article
Chemistry, Analytical
Kaixuan Wang, Nuanfei Zhu, Yumo Li, Hu Zhang, Beibei Wu, Jian Cui, Jun Tang, Zhugen Yang, Fang Zhu, Zhen Zhang
Summary: In this study, a universal detection system based on a novel polyadenine-based tetrahedral DNA nanostructure was developed, which can rapidly and sensitively detect various targets in different fields.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Lijun Zhao, Jianfei Mao, Li Hu, Shu Zhang, Xiaofeng Yang
Summary: A rapid signal amplified AFB1 detection system based on SRCHA has been developed, which utilizes a catalyzed hairpin assembly reaction to significantly enhance the fluorescence signal for AFB1 detection within 15 minutes with a low detection limit. This system shows great potential for on-site rapid detection of AFB1.
ANALYTICAL METHODS
(2021)
Article
Food Science & Technology
Yu Chen, Fuyuan Zhang, Ruobing Liu, Minxuan Liu, Yaxin Sang, Shuo Wang, Xianghong Wang
Summary: A novel and sensitive aptasensor for AFB1 was developed using an Exo III-integrated signal amplification strategy. The method achieved a low detection limit, wide detection range, and demonstrated good performance in complex matrices. The recovery rate and RSD in cereal samples were comparable to or better than previously reported methods, showing the potential of this sensing method for practical applications.
Article
Spectroscopy
Hongyan Zhang, Weiwei Mao, Yijin Hu, Xiaohong Wei, Lishan Huang, Shen Fan, Mingqing Huang, Yu Song, Yuyan Yu, FengFu Fu
Summary: A highly sensitive and specific visual detection method for aflatoxin B1 (AFB1) has been developed based on the combination of aptamer, rolling circle amplification (RCA), and enzyme catalysis. The method can achieve a wide linear detection range and low detection limit, and exhibits excellent specificity in complex sample matrices, showing great potential for practical AFB1 detection.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Review
Chemistry, Analytical
Liping Zhao, Linsen Li, Yi Zhao, Chao Zhu, Ruiqi Yang, Mengqi Fang, Yunxia Luan
Summary: Point-of-care testing (POCT) has become popular in a wide range of promising applications due to its low cost, ease of use, simple result interpretation, and reliability. This review summarizes the methods of aptamer selection for small molecules, the design principles of aptasensors, and different types of portable devices, and discusses future directions in aptamer selection, aptasensor design, high throughput, and simultaneous detection.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Shan Huang, Lumin Wang, Chusheng Huang, Jiangning Xie, Wei Su, Jiarong Sheng, Qi Xiao
SENSORS AND ACTUATORS B-CHEMICAL
(2015)
Article
Chemistry, Analytical
Shan Huang, Lumin Wang, Chusheng Huang, Baoqing Hu, Wei Su, Qi Xiao
Article
Chemistry, Analytical
Shan Huang, Lumin Wang, Chusheng Huang, Wei Su, Qi Xiao
SENSORS AND ACTUATORS B-CHEMICAL
(2016)
Article
Chemistry, Analytical
Shan Huang, Lumin Wang, Chusheng Huang, Wei Su, Qi Xiao
SENSORS AND ACTUATORS B-CHEMICAL
(2017)
Article
Chemistry, Applied
Lumin Wang, Fawei Zhu, Miao Chen, Yuqiu Zhu, Jianbo Xiao, Hua Yang, Xiaoqing Chen
Article
Chemistry, Analytical
Lumin Wang, Fawei Zhu, Sen Liao, Miao Chen, Yu Qiu Zhu, Qi Liu, Xiaoqing Chen
Article
Chemistry, Multidisciplinary
Lumin Wang, Fawei Zhu, Yuqiu Zhu, Siqi Xie, Miao Chen, Yu Xiong, Qi Liu, Hua Yang, Xiaoqing Chen
Article
Chemistry, Analytical
Lumin Wang, Guangjuan Liu, Yuxiang Ren, Yinghui Feng, Xinyi Zhao, Yuqiu Zhu, Miao Chen, Fawei Zhu, Qi Liu, Xiaoqing Chen
ANALYTICAL CHEMISTRY
(2020)