Journal
NEUROPHOTONICS
Volume 6, Issue 1, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.NPh.6.1.015009
Keywords
microscopy; structured illumination; light sheet fluorescence microscopy; optical sectioning
Categories
Funding
- National Institutes of Health [R01NS090645]
- National Science Foundation [1350654]
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1350654] Funding Source: National Science Foundation
Ask authors/readers for more resources
Light sheet fluorescence microscopy (LSFM) is a powerful tool for investigating model organisms including zebrafish. However, due to scattering and refractive index variations within the sample, the resulting image often suffers from low contrast. Structured illumination (SI) has been combined with scanned LSFM to remove out-of-focus and scattered light using square-law detection. Here, we demonstrate that the combination of LSFM with linear reconstruction SI can further increase resolution and contrast in the vertical and axial directions compared to the widely adopted root-mean square reconstruction method while using the same input images. We apply this approach to imaging neural activity in 7-day postfertilization zebrafish larvae. We imaged two-dimensional sections of the zebrafish central nervous system in two colors at an effective frame rate of 7 frames per second. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available