Article
Horticulture
Maria Karen Serrano-Fuentes, Fernando Carlos Gomez-Merino, Serafin Cruz-Izquierdo, Jose Luis Spinoso-Castillo, Jerico Jabin Bello-Bello
Summary: In this study, somaclonal variation in Vanilla planifolia was induced by gamma radiation and detected using ISSR molecular markers. Results showed that low doses of radiation promoted development, while high doses inhibited growth and caused death. UBC-808, UBC-836, and UBC-840 primers exhibited the highest percentage of polymorphism. Genetic distance analysis revealed the presence of somaclonal variation in both irradiated and non-irradiated explants.
Article
Plant Sciences
Maria Teresa Gonzalez-Arnao, Carlos A. Cruz-Cruz, Fabiola Hernandez-Ramirez, Jorge A. Alejandre-Rosas, Ana Constanza Hernandez-Romero
Summary: This study compared the impact of different cryogenic techniques on the vegetative growth of vanilla plants, finding a significant increase in leaf number in cryo-derived plants with the V-Cp method, and a high level of genetic stability.
Article
Agronomy
Danuta Wojcik, Aleksandra Trzewik, Danuta Kucharska
Summary: This study evaluated field performance and genetic stability of micropropagated plants of four gooseberry cultivars, finding that the growth vigor and fruit yield of most MP plants were greater than those of ST plants. Microscopic observations showed higher stomata frequency on MP plants compared to ST plants. Genetic variability assessments using AFLP and ISSR techniques revealed differences among the cultivars, with no polymorphism detected in ST plants.
Article
Plant Sciences
Marco A. Ramirez-Mosqueda, Jerico J. Bello-Bello
Summary: The study compared three different culture systems for the micropropagation of vanilla, with temporary immersion system showing the best results in terms of shoot proliferation and development. The SETIS(TM) bioreactor was found to improve the physiological conditions of in-vitro development and is considered an alternative for large-scale propagation of V. planifolia.
ACTA PHYSIOLOGIAE PLANTARUM
(2021)
Review
Biochemistry & Molecular Biology
Georgiana Duta-Cornescu, Nicoleta Constantin, Daniela-Maria Pojoga, Daniela Nicuta, Alexandra Simon-Gruita
Summary: Cell and tissue plant cultures are widely used in conservation and multiplication of species, especially for economically important plants. In vitro technologies have been developed for medicinal plants to produce secondary metabolites and pathogen-free plants. However, caution must be taken to balance maintaining genetic fidelity while exploring somaclonal variations for useful traits. This review focuses on somaclonal variations in medicinal plants from the perspectives of cytogenetics, genetics, and epigenetics, with emphasis on the subtle changes at the genetic and epigenetic level using DNA markers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Mi Li, Yunqiao Pu, Xianzhi Meng, Fang Chen, Richard A. Dixon, Arthur J. Ragauskas
Summary: This study revealed a particularly high level of tricin-lignin in vanilla plant, especially in the aerial roots, which could play a vital role in valorization of lignin and offer new ways to design and engineer lignin structures for improved plant properties.
Review
Plant Sciences
Renatha Tavares de Oliveira, Joana Paula da Silva Oliveira, Andrea Furtado Macedo
Summary: This review article discusses the importance of the vanilla crisis and the potential of vanilla crop wild relatives as alternatives. The metabolic characterization and potential flavor compounds of these wild relatives are highlighted.
Article
Biodiversity Conservation
Agustin Maceda, Adriana Delgado-Alvarado, Victor M. Salazar-Rojas, B. Edgar Herrera-Cabrera
Summary: This study aimed to investigate the distribution and morphological variation of Vanilla planifolia in the Huasteca region of Hidalgo. The results showed the distribution of the vanilla populations and identified five different morphotypes based on labellum characteristics.
Article
Plant Sciences
Jehan Salem, Ahmed Hassanein, Deiaa A. El-Wakil, Naglaa Loutfy
Summary: The study found that the water potential level and medium type have significant effects on the shoot number and growth morphology of paulownia plantlets, with long-term culture leading to high genetic variation. ISSR technique showed higher polymorphism than RAPD, and NaCl application further increased polymorphism.
Article
Biotechnology & Applied Microbiology
Karolina Nowakowska, Anna Pinkowska, Ewa Siedlecka, Andrzej Pacholczak
Summary: The study found that different cytokinins treatments had significant effects on shoot proliferation in 'Kazimierz Odnowiciel', with the ZEA+2iP combination showing the best growth performance. The addition of cytokinins not only increased chlorophyll, carotenoids, and total soluble sugar contents, but also enhanced plant defense against oxidative stress in tissue culture.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Biochemistry & Molecular Biology
Hui Wang, Xiao-Qian Wang, Zhi-Lan Zeng, Huan Yu, Wei Huang
Summary: This study investigated the photosynthetic dynamics of the CAM orchid Vanilla planifolia under fluctuating light. The results showed that the light use efficiency was higher in the morning compared to the afternoon. During photosynthetic induction, electron flow through photosystem I reached its maximum value rapidly. A rapid re-oxidation of P700 was observed upon transition from dark to actinic light, indicating the effective utilization of the water-water cycle. In the afternoon, cyclic electron flow was stimulated to optimize photosynthesis.
Article
Multidisciplinary Sciences
Eduardo Martinez-Santos, Carlos Alberto Cruz-Cruz, Jose Luis Spinoso-Castillo, Jerico Jabin Bello-Bello
Summary: The study focused on evaluating the morphological, physiological, and biochemical responses of Vanilla planifolia under in vitro water stress conditions induced with polyethylene glycol (PEG). It was found that increasing PEG concentration led to a decrease in growth and chlorophyll content, while dry matter, proline, and glycine betaine contents increased. The results suggest that PEG-induced osmotic stress can help in understanding the physiological and biochemical mechanisms of response to water stress, and Pro and GB osmolytes can be used as potential markers in breeding programs for water stress tolerance.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Jaturon Thipwong, Kittima Kongton, Sainiya Samala
Summary: This research investigated the micropropagation of the economically important orchid Doritis pulcherrima using in vitro culture. The study found the suitable medium and plant growth regulators concentration for protocorm-like body formation and plantlet regeneration. It also observed somaclonal variation after prolonged subculture of the plantlets.
PLANT BIOTECHNOLOGY REPORTS
(2022)
Article
Ecology
Ireri A. Carbajal-Valenzuela, Ariel H. Munoz-Sanchez, Juan Hernandez-Hernandez, Francisco Barona-Gomez, Camille Truong, Angelica Cibrian-Jaramillo
Summary: The study investigated the differences in endophytic microbiomes between symptomatic and asymptomatic vanilla plants, showing the presence of specific core microbiota in roots and stems. However, there were no significant differences in microbial community richness among feral, asymptomatic, and symptomatic cultivated vanilla. The findings lay the groundwork for a better understanding of the role of the microbiome in vanilla fungal diseases.
Article
Food Science & Technology
Oscar Antonio-Gutierrez, Isidro Pacheco-Reyes, Luicita Lagunez-Rivera, Rodolfo Solano, Maria del Pilar Canizares-Macias, Gerard Vilarem
Summary: This research evaluated the application of microwave and ultrasound during the killing stage of the Vanilla planifolia pod curing process and its effect on vanillin content and beta-glucosidase activity. The results show that the application of these technologies significantly improves vanillin yield and reduces the curing time to 20 days.
Article
Plant Sciences
Marco A. Ramirez-Mosqueda, Jerico J. Bello-Bello
Summary: The study compared three different culture systems for the micropropagation of vanilla, with temporary immersion system showing the best results in terms of shoot proliferation and development. The SETIS(TM) bioreactor was found to improve the physiological conditions of in-vitro development and is considered an alternative for large-scale propagation of V. planifolia.
ACTA PHYSIOLOGIAE PLANTARUM
(2021)
Article
Plant Sciences
Lourdes Delgado-Aceves, Maria Teresa Gonzalez-Arnao, Fernando Santacruz-Ruvalcaba, Raquel Folgado, Liberato Portillo
Summary: This study focused on indirect somatic embryogenesis and cryopreservation of Agave tequilana Weber cultivar 'Chato'. The optimal conditions for somatic embryo formation were identified, and the highest post-cryopreservation regrowth and plant conversion were achieved using PVS2 at 0°C for 15 min. Overall, this work provides new insights into somatic embryogenesis in Agave and reports the first results on cryopreservation of somatic embryos in this species.
Article
Biotechnology & Applied Microbiology
Jerico Jabin Bello-Bello, Sandra Schettino-Salomon, Javier Ortega-Espinoza, Jose Luis Spinoso-Castillo
Summary: The study evaluated the efficiency of temporary immersion system for micropropagation of pitahaya, with the best culture system being immersion for 2 minutes every 4 hours with 15 explants per bioreactor. High survival rates of 98%-100% were achieved during acclimatization.
Article
Agriculture, Dairy & Animal Science
Miguel Angel Matus-Aragon, Fernando Gonzalez-Ceron, Josafhat Salinas-Ruiz, Eliseo Sosa-Montes, Arturo Pro-Martinez, Omar Hernandez-Mendo, Juan Manuel Cuca-Garcia, David Jesus Chan-Diaz
Summary: The study evaluated the productive performance and nutrient utilization of Mexican Creole chickens fed with different diets. Results showed that a diet with 2,550 kcal ME and 17% CP maintained good productive parameters and improved retention efficiency of CP. This suggests that the Mexican Creole chickens can be successfully fed with this diet without compromising their productivity.
Article
Plant Sciences
Jose Luis Spinoso-Castillo, Juan Antonio Perez-Sato, Sandra Silvana Schettino-Salomon, Jerico Jabin Bello-Bello
Summary: This study evaluated the efficiency of gibberellin inhibitors, paclobutrazol (PBZ) and trinexapac-ethyl (TNE), for in vitro conservation in anthurium, sugarcane, and agave. The results showed that 1 mg L-1 PBZ was the best treatment for anthurium, while 1 mg L-1 PBZ and 3 mg L-1 PBZ or TNE were most effective for sugarcane and agave. The gibberellin-inhibiting compounds PBZ and TNE are low-cost alternatives for in vitro conservation and can be evaluated in other species.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2022)
Article
Biotechnology & Applied Microbiology
Lourdes Delgado-Aceves, Liberato Portillo, Raquel Folgado, Felipe de Jesus Romo-Paz, Maria Teresa Gonzalez-Arnao
Summary: This study successfully developed supplementary strategies for micropropagation and cryopreservation of Agave peacockii, providing new viable methods for the conservation of this endangered species.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Plant Sciences
Maria Teresa Gonzalez-Arnao, Carlos A. Cruz-Cruz, Fabiola Hernandez-Ramirez, Jorge A. Alejandre-Rosas, Ana Constanza Hernandez-Romero
Summary: This study compared the impact of different cryogenic techniques on the vegetative growth of vanilla plants, finding a significant increase in leaf number in cryo-derived plants with the V-Cp method, and a high level of genetic stability.
Article
Plant Sciences
Javier Emanuel Bulbarela-Marini, Fernando Carlos Gomez-Merino, Maria Elena Galindo-Tovar, Miriam Cristina Pastelin-Solano, Joaquin Murguia-Gonzalez, Rosalia Nunez-Pastrana, Odon Castaneda-Castro
Summary: This study aims to identify an in vitro culture system for the propagation of disease-free Persian lime clones with genetic stability. The RITA system showed the highest number of shoots and the lowest somaclonal variation, indicating its effectiveness in maintaining genetic stability during subcultures.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Food Science & Technology
Cardenas-Cagal Angel, Herrera-Corredor J. Andres, Josafhat Salinas-Ruiz, Francisco Osorio-Acosta, Francisco Hernandez-Rosas
Summary: This study aimed to explore preferences for lemon consumption and the different reactions consumers show in terms of basic needs, emotions, and attitudes. The results revealed that juiciness was the most valued attribute, and fresh lemon was the most preferred form of consumption. Age, occupation, and frequency of consumption were found to be significant factors in lemon consumption. Females and people over 40 years of age showed more positive attitudes towards the importance of lemon consumption.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Plant Sciences
Carlos A. Cruz-Cruz, Maria T. Gonzalez-Arnao, Jose R. Bautista-Aguilar, Marco A. Ramirez-Mosqueda
Summary: This study aimed to establish an efficient protocol for the in vitro conservation of Stanhopea tigrina. The effects of various plant growth inhibitors and osmoregulators were evaluated, and it was found that 2 mg L-1 ABA had the lowest impact on shoot height, shoot number, and root number of S. tigrina. During the regeneration process, 2 mg L-1 BAP achieved the regeneration of 3.2 shoots per explant and promoted root growth. The acclimatization process resulted in a plant survival rate of 80%.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Genetics & Heredity
Osval A. A. Montesinos-Lopez, Alison R. R. Bentley, Carolina Saint Pierre, Leonardo Crespo-Herrera, Josafhat Salinas Ruiz, Patricia Edwigis Valladares-Celis, Abelardo Montesinos-Lopez, Jose Crossa
Summary: Genomic selection (GS) is a revolutionary plant breeding method that allows the selection of candidate genotypes without the need for field phenotypic evaluation. This study investigated the genomic prediction accuracy of wheat hybrids by incorporating covariates with parental phenotypic information into the model. The results showed that the models with parental information outperformed those without parental information, and the inclusion of covariates significantly improved prediction accuracy compared to marker information. However, the use of parental phenotypic information as covariates is expensive and not always available.
Article
Biotechnology & Applied Microbiology
Spinoso-Castillo Jose Luis, Bello-Bello Jerico Jabin
Summary: The research aimed to study the physiological and biochemical mechanisms of V. planifolia during in vitro multiplication using a photomixotrophic system. Results showed that the best development parameters were obtained with 30 g L-1 sucrose + CO2 treatment. The study provides a better understanding of photomixotrophism in vanilla and can be applied to other species.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Plant Sciences
Edgar Garcia-Ramirez, Adriana Contreras-Oliva, Josafhat Salinas-Ruiz, Gabriela Hernandez-Ramirez, Jose Luis Spinoso-Castillo, Saira Itzel Colmenares Cuevas
Summary: Natural alternatives, such as cinnamon essential oil, neem oil, and black sapote fruit extract, were evaluated for their in vitro inhibition of fungi isolated from chayote fruit. Cinnamon oil showed fungicidal effects, while neem oil and black sapote extract demonstrated varying degrees of growth inhibition for different fungi at specific concentrations. Applying these plant extracts at appropriate concentrations can effectively prevent or reduce postharvest fungal infections on chayote fruit.
Article
Agronomy
Sherell Zamora Juarez, Daniel Arturo Rodriguez-Lagunes, Francisco Osorio-Acosta, Odon Castaneda-Castro, Rosalia Nunez-Pastrana, Juan Carlos Noa-Carrazana
Summary: In commercial citrus orchards in Martinez de la Torre, Veracruz, Mexico, there is evidence of resistance in Diaphorina citri to chlorpyrifos and dimethoate. The study showed that the mortality rates of D. citri exposed to dimethoate and chlorpyrifos were lower compared to the control group, indicating some level of resistance to organophosphate insecticides in the area. This information can be valuable in determining the appropriate insecticides to use for pest control.
REVISTA DE LA FACULTAD DE AGRONOMIA DE LA UNIVERSIDAD DEL ZULIA
(2021)
Article
Plant Sciences
Gisela Carrera-Alvarado, Maria de Lourdes Arevalo-Galarza, Joel Velasco-Velasco, Gumercindo Honorato de-la-Cruz-Guzman, Josafhat Salinas-Ruiz, Obdulia Baltazar-Bernal
Summary: This study found that wax coating could effectively reduce chilling injury and prolong the vase life of Heliconia wagneriana stems stored at low temperature. However, there was no significant effect observed for the use of 1 mM salicylic acid.
ORNAMENTAL HORTICULTURE-REVISTA BRASILEIRA DE HORTICULTURA ORNAMENTAL
(2021)
