Article
Chemistry, Multidisciplinary
Feng Chen, Shiming Wu, Jingjing Fu, Xiaohui Lv, Huihui Chai, Lixia Gao, Ling Yu
Summary: This study fabricated rough PDMS surfaces with microcavity structures by replicating from a sandpaper template and investigated their impact on cell anchoring and growth. The results showed that rough PDMS could significantly support more cell growth compared to smooth PDMS. The study also compared the efficacy of physical topology-enhanced cell compatibility with chemically functionalized PDMS and found comparable results. Moreover, the study highlighted the potential of sandpaper templating in building microdevices for on-chip cell culture.
NEW JOURNAL OF CHEMISTRY
(2022)
Review
Oncology
Emma Schuster, Rokana Taftaf, Carolina Reduzzi, Mary K. Albert, Isabel Romero-Calvo, Huiping Liu
Summary: CTC clusters play a crucial role in cancer, with a higher metastatic potential and unfavorable clinical outcomes compared to single CTCs. While the mechanism of CTC cluster formation is still unclear, certain cell adhesion molecules and tight junction proteins have been identified as key factors.
Article
Engineering, Electrical & Electronic
Yong Wang, Qian Zhang, Ran Tao, Dongyang Chen, Jin Xie, Hamdi Torun, Linzi E. Dodd, Jingting Luo, Chen Fu, Jethro Vernon, Pep Canyelles-Pericas, Richard Binns, Yongqing Fu
Summary: A rapid and controllable acoustothermal microheater using AlN/Si thin film SAWs was proposed in this paper, showing superior performance compared to other types of thin film SAW devices. The dynamic heating processes of the device for both sessile droplets and liquid within a microchamber were studied, demonstrating stable and repeatable temperature control. Standing SAW heating exhibited improved temperature uniformity compared to travelling SAWs, suggesting great potential for microfluidic heating applications.
SENSORS AND ACTUATORS A-PHYSICAL
(2021)
Review
Biotechnology & Applied Microbiology
Sahar Asghari, Fatemeh Barati, Manizheh Avatefi, Matin Mahmoudifard
Summary: The majority of cancer-related deaths are attributed to metastasis, which involves the circulation of circulating tumor cells (CTCs) in the body. Detecting and counting these cells is challenging due to their rarity, but crucial for early diagnosis and treatment. Nanomaterials offer a promising approach for capturing and releasing CTCs, potentially surpassing traditional detection methods.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2021)
Review
Chemistry, Analytical
Fulai Li, Hengyi Xu, Yufen Zhao
Summary: As a new type of liquid biopsy, circulating tumor cell (CTC) detection and analysis offer the possibility of real-time monitoring of metastatic development, cancer recurrence, and therapeutic efficacy. The rarity and heterogeneity of CTCs present great challenges, and magnetic particle-based methods show promise for effective CTC capture. Progress has been made in utilizing magnetic particles to enhance CTC capture, which is crucial for cancer diagnosis and prognosis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Reef Einoch-Amor, Yoav Y. Broza, Hossam Haick
Summary: The study introduces an artificially intelligent nanoarray using chemisensitive nanostructured films for detecting single cancer cells in blood samples, showing high accuracy, sensitivity, and specificity. The technology is not affected by the medium or environment, and could potentially be used for early cancer detection and continuous monitoring.
Article
Oncology
Nerymar Ortiz-Otero, Jocelyn R. Marshall, Antonio Glenn, Jubin Matloubieh, Jean Joseph, Deepak M. Sahasrabudhe, Edward M. Messing, Michael R. King
Summary: The study revealed that radical surgery for localized prostate cancer can lead to tumor cell mobilization into the bloodstream, with these cells returning to baseline levels within a few weeks. In patients experiencing cancer recurrence, levels of circulating tumor cells and cancer-associated fibroblasts gradually increase over time. An experimental TRAIL-based therapy was shown to induce apoptosis in tumor cells in blood samples ex vivo, suggesting a potential new adjuvant therapy in combination with surgery.
Article
Polymer Science
Sahar Asghari, Matin Mahmoudifard
Summary: A smart system is needed to identify the initiation of metastasis efficiently in order to prevent an increase in deaths due to the metastasis of primary tumors in many cancers. The early detection of cancer and using lower doses of drugs can lead to more efficient treatment with lower side effects, as shown by the efficacy of core-shell NFMs in capturing and releasing cancerous cells.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2021)
Article
Oncology
Maria Spiliotaki, Christiana Michael Neophytou, Paris Vogazianos, Ioannis Stylianou, Gregoria Gregoriou, Andreas Ioannou Constantinou, Constantinos Deltas, Haris Charalambous
Summary: This study investigated the expression of programmed cell death protein ligand-1 (PD-L1) and marker of proliferation Ki-67 (Ki67) in circulating tumor cells (CTCs) of non-small cell lung cancer (NSCLC) patients treated with pembrolizumab. The results showed that PD patients had higher levels of PD-L1 and Ki67 expression after the first treatment cycle, while DC patients had reduced expression levels. The study also found that PD-L1 positive patients with high Ki67 expression had shorter survival. These findings suggest that monitoring PD-L1 and Ki67 expression in CTCs may predict the efficacy of pembrolizumab.
MOLECULAR ONCOLOGY
(2023)
Article
Oncology
Zhen Tan, Josephine Mun-Yee Ko, Valen Zhuoyou Yu, Ka-On Lam, Dora Lai-Wan Kwong, Ian Yu-Hong Wong, Fion Siu-Yin Chan, Claudia Lai-Yin Wong, Kwan-Kit Chan, Tsz-Ting Law, Faith Sin-Fai Choy, Hoi-Yan Ng, Simon Ying-Kit Law, Maria Li Lung
Summary: By detecting multiple cancer driver genes associated with epithelial-to-mesenchymal transition (EMT) at the transcript level, we investigated the clinical significance of circulating tumor cells (CTCs) in cancer progression. The study found significant correlations between the presence of CTCs and tumor size, cancer stages, and metastasis. Furthermore, high mRNA levels of certain genes were associated with shorter overall survival in esophageal squamous cell carcinoma (ESCC) patients.
Review
Oncology
Anna Szczerba, Aleksandra Sliwa, Pawel P. Pieta, Anna Jankowska
Summary: This review focuses on the biology of ovarian circulating tumor cells (CTCs) and their role in the diagnosis and therapy of ovarian cancer.
Review
Biochemistry & Molecular Biology
Patricia Mondelo-Macia, Jorge Garcia-Gonzalez, Luis Leon-Mateos, Adrian Castillo-Garcia, Rafael Lopez-Lopez, Laura Muinelo-Romay, Roberto Diaz-Pena
Summary: Lung cancer is a leading cause of mortality worldwide, with small cell lung cancer being characterized by aggressiveness and short-lived treatment effects. Liquid biopsy has emerged as a new tool for the management of SCLC patients in recent years.
Article
Polymer Science
Jisu Kim, U. Pramod Kumar, Sung-Jun Lee, Chang-Lae Kim, Jeong-Won Lee
Summary: Modifying surface wettability has been widely practiced in engineering for functional purposes. This study focuses on the fabrication and characterization of fluorine-free superhydrophobic coatings using varying ratios of PDMS and toluene. The results showed that the coating's roughness, wettability, and durability can be controlled by viscosity and surface structure. Furthermore, the superhydrophobic coatings exhibited great durability in saline solutions, but degradation was observed in acidic and alkaline solutions.
Article
Oncology
Lucia Tombolan, Elisabetta Rossi, Andrea Binatti, Angelica Zin, Mariangela Manicone, Antonella Facchinetti, Silvia Lucchetta, Maria Carmen Affinita, Paolo Bonvini, Stefania Bortoluzzi, Rita Zamarchi, Gianni Bisogno
Summary: Liquid biopsy analysis is a powerful and noninvasive tool for uncovering molecular features and disease progression in pediatric rhabdomyosarcoma patients. The study found that patients with metastatic disease have a higher number of circulating tumor cells compared to those with localized disease, while the detection of disseminated tumor cells is independent of disease presentation. Liquid biopsy can also provide information about the molecular makeup of primary tumors.
MOLECULAR ONCOLOGY
(2022)
Article
Oncology
Yuichiro Miki, Masakazu Yashiro, Kenji Kuroda, Tomohisa Okuno, Shingo Togano, Go Masuda, Hiroaki Kasashima, Masaichi Ohira
Summary: This study found that a high number of CEA-positive CTCs in patients with gastric cancer were associated with a lower 3-year recurrence-free survival rate. CEA-positive CTCs could serve as a clinically useful biomarker for patients with gastric cancer.
Article
Chemistry, Analytical
Jingxin Liu, Mohammed A. A. Abdullah, Liwei Yang, Jun Wang
ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Wenning Jiang, Lulu Han, Liwei Yang, Ting Xu, Jiabei He, Ruilian Peng, Ziyu Liu, Chong Zhang, Xiaomin Yu, Lingyun Jia
Article
Nanoscience & Nanotechnology
He Sun, Rui Li, Huijie Li, Zhengyan Weng, Guangfu Wu, Peter Kerns, Steven Suib, Xueju Wang, Yi Zhang
Summary: This paper introduces a bioinspired oil-infused slippery surface with excellent waterproof performance, which can serve as encapsulation materials for medical devices, underwater electronics, and more. Experimental tests and reaction-diffusion modeling reveal the key waterproof properties of this surface.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Huijie Li, Guangfu Wu, Zhengyan Weng, He Sun, Ravi Nistala, Yi Zhang
Summary: Electrolytes are crucial for regulating various bodily functions, but current monitoring methods are often inconvenient. A minimally invasive microneedle-based potentiometric sensing system has been developed for multiplexed and continuous monitoring of Na(+)and K+ in skin interstitial fluids, showing promising experimental results.
Article
Multidisciplinary Sciences
Guangfu Wu, Ian Heck, Nannan Zhang, Glenn Phaup, Xincheng Zhang, Yixin Wu, David E. Stalla, Zhengyan Weng, He Sun, Huijie Li, Zhe Zhang, Shinghua Ding, De-Pei Li, Yi Zhang
Summary: This study introduces a wireless, programmable push-pull microsystem for membrane-free neurochemical sampling with cellular spatial resolution in freely moving animals. It shows high efficiency in sampling various neurochemicals and captures the release of neuropeptides in freely moving mice. This system provides opportunities for understanding the modulation of diverse behavioral outputs of the brain by neuropeptide release.
Article
Chemistry, Analytical
Revanth Reddy, Liwei Yang, Jesse Liu, Zhuojie Liu, Jun Wang
Summary: This study presents a rapid multiplex method for quantitative detection of protein markers on brain sections. The technology has high sensitivity, resolution, and signal-to-noise level, and is 10 times faster than similar methods. It can be used to quickly and conveniently detect multiple biomarkers on tissue specimens, making it suitable for clinical pathology and disease mechanistic studies.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Huijie Li, Jie Yang, Guangfu Wu, Zhengyan Weng, Yang Song, Yuxuan Zhang, Jeffrey A. Vanegas, Lori Avery, Zan Gao, He Sun, Yupeng Chen, Kevin D. Dieckhaus, Xue Gao, Yi Zhang
Summary: This study reports a new biosensor for amplification-free nucleic acid detection using Cas13a and ultrasensitive graphene field-effect transistors (gFETs). It achieves the detection of SARS-CoV-2 and RSV genome without target preamplifications, even at very low viral loads, making it one of the most sensitive nucleic acid diagnostic platforms to date.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Guangfu Wu, Nannan Zhang, Avi Matarasso, Ian Heck, Huijie Li, Wei Lu, J. Glenn Phaup, Michael J. Schneider, Yixin Wu, Zhengyan Weng, He Sun, Zan Gao, Xincheng Zhang, Stefan G. Sandberg, Dilruba Parvin, Elena Seaholm, Syed Kamrul Islam, Xueju Wang, Paul E. M. Phillips, Daniel C. Castro, Shinghua Ding, De-Pei Li, Michael R. Bruchas, Yi Zhang
Summary: This study presents a soft implantable aptamer-graphene microtransistor probe for real-time monitoring of neurochemical release, demonstrating improved spatial resolution and selectivity with high sensitivity. The developed system shows capability in effectively monitoring dopamine release dynamics and has potential applications in basic neuroscience studies and research on neurological disease processes.
Article
Chemistry, Analytical
Zan Gao, Guangfu Wu, Yang Song, Huijie Li, Yuxuan Zhang, Michael J. Schneider, Yingqi Qiang, Jackson Kaszas, Zhengyan Weng, He Sun, Bryan D. Huey, Rebecca Y. Lai, Yi Zhang
Summary: This study presents a flexible neural probe for multiplexed neurochemical monitoring using aptamers functionalized on graphene field-effect transistors (G-FETs). The neural probes demonstrate excellent flexibility, lightweight, and can monitor serotonin and dopamine with high sensitivity and selectivity. The developed methodology can be adapted for monitoring other neurochemicals.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Liwei Yang, Avery Ball, Jesse Liu, Tanya Jain, Yue-Ming Li, Firoz Akhter, Donghui Zhu, Jun Wang
Summary: Current single-cell tools are limited in analyzing proteins, but the CycMIST technology allows for comprehensive functional proteome profiling of single cells through multiple rounds of multiplexing.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Yi Li, Guangfu Wu, Gyuho Song, Shao-Hao Lu, Zizheng Wang, He Sun, Yi Zhang, Xueju Wang
Summary: This paper introduces a soft, pressure-tolerant, and flexible electronic sensor that can operate under high hydrostatic pressure and salinity environments without the need for pressure chambers. The sensors show excellent performance in resistive temperature and conductivity measurements, with negligible effect from pressure. Encapsulated with a developed soft oil-infused elastomer, the sensor demonstrates long-term stability in a high salinity water environment.
Article
Nanoscience & Nanotechnology
Yue Cao, Xingchi Chen, Avi Matarasso, Zizheng Wang, Yang Song, Guangfu Wu, Xincheng Zhang, He Sun, Xueju Wang, Michael R. Bruchas, Yan Li, Yi Zhang
Summary: Silicone elastomers are widely used in biomedical research and clinical medicine, but protein adsorption on their surfaces poses a challenge. This study presents a covalently attached slippery surface coating that effectively reduces protein adsorption on silicone elastomer surfaces. The results suggest potential applications of this coating in implantable medical devices, organs-on-chips, and other fields to prevent protein adsorption.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Zhengyan Weng, Zheng You, Huijie Li, Guangfu Wu, Yang Song, He Sun, Ava Fradlin, Camille Neal-Harris, Mengshi Lin, Xue Gao, Yi Zhang
Summary: The CRISPR Cas12a-gFET biosensor array is a novel platform for amplification-free, ultrasensitive, reliable, and highly specific detection of both single-stranded and double-stranded DNA targets. It utilizes the multiturnover trans-cleavage activity of CRISPR Cas12a for intrinsic signal amplification and the ultrasensitivity of gFET. The array of 48 sensors on a single chip enhances data reliability and enables the discrimination of single-nucleotide polymorphisms.
Article
Chemistry, Physical
Yi Li, Andres Villada, Shao-Hao Lu, He Sun, Jianliang Xiao, Xueju Wang
Summary: Traditional rigid ocean pressure sensors can be replaced by soft, flexible pressure sensors that measure a wide range of hydrostatic pressures in harsh ocean environments. These sensors are made of lithographically patterned gold thin films encapsulated with a soft Parylene C film. Studies show that the pressure sensors have high consistency, negligible hysteresis, and excellent flexibility, making them promising for integration with various platforms such as animal tags and soft underwater robotics.
Article
Biochemical Research Methods
Mohammed A. A. Abdullah, Nooshin Amini, Liwei Yang, Janet L. Paluh, Jun Wang