Article
Engineering, Biomedical
Xinyu Niu, Yuguo Xia, Lei Luo, Yu Chen, Ji Yuan, Juntao Zhang, Xianyou Zheng, Qing Li, Zhifeng Deng, Yang Wang
Summary: Research shows that inducible pluripotent stem cell-derived small extracellular vesicles (iPSC-sEVs) can alleviate microglia senescence and improve the outcome of aged stroke.
MATERIALS TODAY BIO
(2023)
Article
Immunology
Rinal Sahputra, Krittee Dejyong, Adrian S. Woolf, Matthias Mack, Judith E. Allen, Dominik Ruckerl, Sarah E. Herrick
Summary: Peritoneal adhesions commonly occur after abdominal or pelvic surgery and there are currently no effective treatments available. Peritoneal macrophages have complex and nuanced responses after surgery, and differences in response among specific macrophage subpopulations partially explain the risk of developing peritoneal scars. In a mouse experiment, it was found that monocyte-derived macrophages play an important protective role in reducing adhesion formation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Luiza Ghila, Thomas Aga Legoy, Andreas Froslev Mathisen, Shadab Abadpour, Joao A. Paulo, Hanne Scholz, Helge Raeder, Simona Chera
Summary: Research in the past decade has shown that changes in cell identity, such as dedifferentiation or transdifferentiation, accompany the decline of insulin-producing beta cells in most diabetes conditions. External glucose levels have a concentration-dependent impact on different protein subsets in human pancreatic progenitors, influencing cell identity through redox balance regulation. High glucose levels lead to both beneficial and detrimental changes in achieving the desired islet cell fingerprint, with only a subgroup of effects and pathways regulated by changes in redox balance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Lulu Zhang, Xiuteng Zhou, Huijian Chen, Liangzhen You, Ting Zhang, Meng Cheng, Yudi Yao, Xin Pan, Xinzhou Yang
Summary: Mulberry (Morus alba L.) is not only a tasty food but also a beneficial medicinal substance that has been historically used to treat diabetes. Recent research has shown that the ethyl acetate extract of Morus alba L. fruits (EMF) has hypoglycemic and hypolipidemic properties, but the specific mechanisms are unclear.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Cell Biology
Agnes Badu-Mensah, Paola Valinski, Hemant Parsaud, James J. Hickman, Xiufang Guo
Summary: This study investigated the effect of hyperglycemia on human myoblast physiology using iPSC-derived myoblasts, and found that hyperglycemic myoblasts showed impaired proliferation, cell cycle progression, and mitochondrial function. The differentiation of hyperglycemic myoblasts resulted in hypertrophic and branched myotubes with disturbed myosin heavy chain arrangement, and the myofibers derived from hyperglycemic myoblasts showed increased fatigability.
Article
Medicine, Research & Experimental
Janis Kerkering, Bakhrom Muinjonov, Kamil S. Rosiewicz, Sebastian Diecke, Charlotte Biese, Juliane Schiweck, Claudia Chien, Dario Zocholl, Thomas Conrad, Friedemann Paul, Marlen Alisch, Volker Siffrin
Summary: Multiple sclerosis (MS) is a common chronic central nervous system inflammatory disease with variable clinical courses. Researchers investigated the mechanisms in benign MS (BMS) compared with progressive MS (PMS) using induced pluripotent stem cells (iPSCs). They found that BMS astrocytes exhibited less axonal damage and upregulated neuronal resilience pathways, accompanied by differential growth factor expression. Furthermore, cocultures of BMS astrocytes and neurons showed neuroprotective effects against inflammatory-induced neurite damage through the expression of specific growth factors and JAK-STAT activation.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Cell Biology
Xingyu Li, Heng Zhang, Xuemeng Wang, Meng Lu, Qianqian Ding, Alex F. Chen, Meng Xiang, Sifeng Chen
Summary: Exosomes derived from induced pluripotent stem cells (iPSCs) have shown to enhance angiogenesis in naturally aged mice, improve antioxidant capacity and cell proliferation, and reduce aging gene expression. These findings suggest that iPSC-derived exosomes may rejuvenate the vascular system and have anti-aging effects.
Article
Cell Biology
Karo Talvio, Rimante Minkeviciene, Kayla G. Townsley, Venkat Swaroop Achuta, Laura M. Huckins, Padraic Corcoran, Kristen J. Brennand, Maija L. Castren
Summary: This study compared the transcriptomes of human neural progenitors (NPCs) generated from patient-derived induced pluripotent stem cells (iPSCs) of three fragile X syndrome (FXS) and three control male donors. The results showed altered expression of several genes related to triplet repeat instability, RNA splicing, testes development, and pathways previously shown to be affected in FXS. The study also revealed disturbed growth linked to reduced expression of LYNX1, suggesting dysregulated cholinergic system. The findings highlight the potential of human iPSCs in disease modeling and therapeutic interventions for FXS.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
David R. Martinez, Alexandra Schafer, Sarah R. Leist, Gabriela De la Cruz, Ande West, Elena N. Atochina-Vasserman, Lisa C. Lindesmith, Norbert Pardi, Robert Parks, Maggie Barr, Dapeng Li, Boyd Yount, Kevin O. Saunders, Drew Weissman, Barton F. Haynes, Stephanie A. Montgomery, Ralph S. Baric
Summary: The emergence of SARS-CoV in 2003 and SARS-CoV-2 in 2019 has led to the development of universal vaccination strategies against the broader Sarbecovirus subgenus. Through the use of chimeric spike designs, high levels of broadly protective neutralizing antibodies have been induced against various high-risk Sarbecoviruses, showing potential for preventing SARS-like zoonotic coronavirus infections with pandemic potential.
Article
Biochemistry & Molecular Biology
Minu Chaudhuri, Ujjal K. Singha, Boden H. Vanderloop, Anuj Tripathi, W. David Nes
Summary: This study reports the lethal effects of two natural product antimetabolites on T. brucei and demonstrates their potential as antitrypanosomal drugs for the treatment of human African trypanosomiasis.
Review
Cell Biology
Kristina G. Maxwell, Jeffrey R. Millman
Summary: Improved stem cell-derived pancreatic islet differentiation protocols can robustly generate insulin-secreting beta cells from patient iPSCs, enabling in vitro disease modeling and the development of autologous diabetes cell replacement therapy. Combining disease modeling with gene editing and sequencing reveals the impact of diabetes-causing mutations on various islet cell types.
CELL REPORTS MEDICINE
(2021)
Article
Immunology
Jason Kang, Margalida Mateu-Borras, Hunter L. Monroe, Emel Sen-Kilic, Sarah Jo Miller, Spencer R. Dublin, Annalisa B. Huckaby, Evita Yang, Gage M. Pyles, Mason A. Nunley, Josh A. Chapman, Md Shahrier Amin, F. Heath Damron, Mariette Barbier
Summary: Pseudomonas aeruginosa is a common cause of hospital-acquired infections. Developing antibodies against this pathogen has shown potential in reducing bacterial burden and preventing infection. The use of ammonium metavanadate resulted in the development of two antibodies that directly reduced the viability of P. aeruginosa and mediated bacterial agglutination.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Endocrinology & Metabolism
Shicheng Sun, Hongkui Deng
Summary: We present a novel abdominal infusion transplantation protocol that allows for the survival, maturation, and functionality maintenance of human pluripotent stem cell-derived pancreatic islets (PSC-islets) in diabetic monkeys.
Article
Biotechnology & Applied Microbiology
Fatemeh Ashrafian, Shahrbanoo Keshavarz Azizi Raftar, Arezou Lari, Arefeh Shahryari, Sara Abdollahiyan, Hamid Reza Moradi, Morteza Masoumi, Mehdi Davari, Shohreh Khatami, Mir Davood Omrani, Farzam Vaziri, Andrea Masotti, Seyed Davar Siadat
Summary: The study showed that pasteurized A. muciniphila and its EVs have preventive effects on HFD-induced obesity, with more noticeable effects than its active form. These treatments also inhibited inflammation and gene expression changes related to lipid metabolism caused by HFD, indicating a potential role in regulating metabolic functions to prevent obesity.
MICROBIAL CELL FACTORIES
(2021)
Article
Biochemical Research Methods
Ishan Goswami, Eleonora de Klerk, Phichitpol Carnese, Matthias Hebrok, Kevin E. Healy
Summary: This article introduces a scalable, multiplexed islet beta MPS device for culturing and testing stem cell-derived enriched beta clusters (eBCs) in MPS, assessing their functionality and drug responses; confirming a glycolytic bottleneck and reactions to long-term drug exposure.
Article
Cell Biology
Jaime Santo-Domingo, Antonio Nunez Galindo, Ornella Cominetti, Umberto De Marchi, Pedro Cutillas, Loic Dayon, Andreas Wiederkehr
CELL COMMUNICATION AND SIGNALING
(2019)
Article
Cell & Tissue Engineering
Laura Lukjanenko, Sonia Karaz, Pascal Stuelsatz, Uxia Gurriaran-Rodriguez, Joris Michaud, Gabriele Dammone, Federico Sizzano, Omid Mashinchian, Sara Ancel, Eugenia Migliavacca, Sophie Liot, Guillaume Jacot, Sylviane Metairon, Frederic Raymond, Patrick Descombes, Alessio Palini, Benedicte Chazaud, Michael A. Rudnicki, C. Florian Bentzinger, Jerome N. Feige
Article
Multidisciplinary Sciences
Massoud Vosough, Francesco Ravaioli, Mihaela Zabulica, Miriam Capri, Paolo Garagnani, Claudio Franceschi, Julie Piccand, Marine R. -C. Kraus, Kristina Kannisto, Roberto Gramignoli, Stephen C. Strom
Article
Endocrinology & Metabolism
Julie Piccand, Constance Vagne, Florence Blot, Aline Meunier, Anthony Beucher, Perrine Strasser, Mari L. Lund, Sabitri Ghimire, Laure Nivlet, Celine Lapp, Natalia Petersen, Maja S. Engelstoft, Christelle Thibault-Carpentier, Celine Keime, Sara Jimenez Correa, Valerie Schreiber, Nacho Molina, Thue W. Schwartz, Adele De Arcangelis, Gerard Gradwohl
MOLECULAR METABOLISM
(2019)
Review
Pharmacology & Pharmacy
Umberto De Marchi, Silvia Fernandez-Martinez, Sergio de la Fuente, Andreas Wiederkehr, Jaime Santo-Domingo
Summary: Pancreatic beta-cells play a central role in regulating glucose homeostasis, with mitochondria playing a crucial role in linking nutrient metabolism to insulin secretion. Mitochondria, as double-membrane organelles with various channels, regulate energy production, signaling, and cell death within beta-cells.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Nutrition & Dietetics
Flavien Bermont, Aurelie Hermant, Romy Benninga, Christian Chabert, Guillaume Jacot, Jaime Santo-Domingo, Marine R-C Kraus, Jerome N. Feige, Umberto De Marchi
Article
Biochemistry & Molecular Biology
Arkadiy A. Bazhin, Riccardo Sinisi, Umberto De Marchi, Aurelie Hermant, Nicolas Sambiagio, Tamara Maric, Ghyslain Budin, Elena A. Goun
NATURE CHEMICAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Laurent Ferrier, Helene Eutamene, Lea Siegwald, Andrea M. Marquard, Valerie Tondereau, Julien Chevalier, Guillaume E. Jacot, Laurent Favre, Vassilia Theodorou, Maria Vicario, Andreas Rytz, Gabriela Bergonzelli, Clara L. Garcia-Rodenas
Summary: The study found that human milk oligosaccharides (HMOs) can impact visceral hypersensitivity (VHS) and alleviate abdominal pain. HMOs have the ability to modulate the microbiota, gut, and brain, playing a role in regulating pain-related functional gastrointestinal disorders.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Nadege Zanou, Haikel Dridi, Steven Reiken, Tanes Imamura de Lima, Chris Donnelly, Umberto De Marchi, Manuele Ferrini, Jeremy Vidal, Leah Sittenfeld, Jerome N. Feige, Pablo M. Garcia-Roves, Isabel C. Lopez-Mejia, Andrew R. Marks, Johan Auwerx, Bengt Kayser, Nicolas Place
Summary: Exercise-induced transient calcium leak through RyR1 causes mitochondrial remodeling to improve respiration.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Anne-Sophie Delbes, Mar Quinones, Cedric Gobet, Julien Castel, Raphael G. P. Denis, Jeremy Berthelet, Benjamin D. Weger, Etienne Challet, Aline Charpagne, Sylviane Metairon, Julie Piccand, Marine Kraus, Bettina H. Rohde, John Bial, Elizabeth M. Wilson, Lise-Lotte Vedin, Mirko E. Minniti, Matteo Pedrelli, Paolo Parini, Frederic Gachon, Serge Luquet
Summary: Peripheral organ circadian clocks, such as the liver clock, can affect the central pacemaker and the overall circadian physiology, offering potential insights into disorders related to altered circadian rhythms.
Article
Biochemistry & Molecular Biology
Anna Weiser, Aurelle Hermant, Flavien Bermont, Federico Sizzano, Sonia Karaz, Pilar Alvarez-Illera, Jaime Santo-Domingo, Vincenzo Sorrentino, Jerome N. Feige, Umberto De Marchi
Summary: Regulation of mitochondrial redox balance is crucial for cell signaling, but the connection between the redox state and cell conditions is not well understood. The study found that activation of the MCU protein modulates mitochondrial redox state, leading to mitochondrial reduction and improved respiratory capacity and mobility. By bypassing MCU, similar benefits can be achieved by directly reducing mitochondrial proteins. This research demonstrates the importance of MCU in regulating mitochondrial redox balance and its effects on respiratory and mobility functions.