Article
Plant Sciences
Lindy A. Allsman, Marschal A. Bellinger, Vivian Huang, Matthew Duong, Alondra Contreras, Andrea N. Romero, Benjamin Verboonen, Sukhmani Sidhu, Xiaoguo Zhang, Holly Steinkraus, Aimee N. Uyehara, Stephanie E. Martinez, Rosalie M. Sinclair, Gabriela Salazar Soriano, Beatrice Diep, V. Dawson Byrd, Alexander Noriega, Georgia Drakakaki, Anne W. Sylvester, Carolyn G. Rasmussen
Summary: In this study, live-cell markers are used to track the dynamic reorganization of microtubules, nuclei, endoplasmic reticulum, and endomembrane compartments during division and the formation of the cell plate in maize leaf epidermal cells. The results show that microtubule dynamicity increases during mitosis, supporting rapid changes in microtubule structures. Additionally, the localization of cell-plate specific proteins was assessed after treatment with specific inhibitors.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Cell Biology
Jennifer M. Roehrl, Rouven Arnold, Karima Djabali
Summary: In Hutchinson-Gilford progeria syndrome (HGPS), NPC clustering mainly occurs in dysmorphic nuclei, with a higher frequency in early passages compared to control cells. Progerin does not disrupt the post-mitotic reassembly of NPCs, but leads to NPC clustering in nuclei with high progerin content. Additionally, nuclear envelope defects during replicative senescence result in NPC clustering in senescent cells with dysmorphic nuclei.
Review
Cell Biology
Gautam Dey, Buzz Baum
Summary: The defining feature of eukaryotic cells, the nucleus, is bounded by a double envelope with nuclear pores critical for separating the genome from the cytoplasm. Eukaryotes have evolved various strategies to remodel the nuclear compartment boundary during mitosis, from disassembling and reassembling the nucleus in daughter cells to maintaining an intact boundary throughout division. This review discusses common features of the division process, topological challenges, and the selective pressures that may drive the evolution of distinct modes of division.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Microbiology
Benjamin Liffner, Sabrina Absalon
Summary: The study utilizes ultrastructure expansion microscopy (U-ExM) to investigate mitosis in the malaria parasite Plasmodium falciparum, revealing various intranuclear microtubule structures. Depletion of MCMBP results in abnormal parasites, which were further analyzed using U-ExM with a newly developed nuclear stain specifically for P. falciparum.
Article
Microbiology
Francisco Alejandro Lagunas-Rangel, Janet Yee, Rosa Maria Bermudez-Cruz
Summary: Giardia duodenalis, a flagellated protozoan responsible for diarrheal disease worldwide, has a complex microtubule cytoskeleton and must coordinate cell division to ensure successful infection through duplication and partitioning of cell structures.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Cell Biology
Alison M. Mills, Carolyn G. Rasmussen
Summary: Cell-division-plane orientation is crucial for the development and growth of plants and animals. TAN1 and AIR9 are microtubule-binding proteins localized at the division site and are necessary for positioning the division plane. The tan1 and air9 single mutants have minor or no noticeable phenotypes, but the double mutant tan1 air9 exhibits synthetic phenotypes including stunted growth, misoriented divisions, and abnormal cell-file rotation in the root differentiation zone. In this study, it was found that TAN1 also plays a role in non-dividing cells and defects in the meristematic zone can affect the organization of elongating and differentiating cells.
JOURNAL OF CELL SCIENCE
(2022)
Review
Cell Biology
Vincent Archambault, Jingjing Li, Virginie Emond-Fraser, Myreille Larouche
Summary: In most animal cell types, the disassembly of the interphase nucleus during mitotic entry is well-studied, but the mechanisms of nuclear reassembly at the end of mitosis are less understood. Recent research has made progress in deciphering the roles of dephosphorylation events in promoting nuclear envelope reassembly, chromosome decondensation, kinetochore disassembly, and interphase chromatin organization. The precise roles and regulation of protein phosphatases in this process, particularly the PP1 and PP2A groups, are being elucidated.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Plant Sciences
M. Arif Ashraf
Summary: This review article focuses on the localization and functional role of nuclear envelope proteins during cell division, with a specific emphasis on the evolution of these proteins in land plant species. While our current knowledge about the localization and function of nuclear envelope proteins during mitosis is limited, more experiments are needed to determine the functional roles of different nuclear envelope proteins in cell division.
Review
Immunology
Anna Selezneva, Alasdair J. Gibb, Dean Willis
Summary: The traditional view of the nuclear envelope as an inert physical barrier has been challenged by recent research, which suggests that it plays important roles in regulating cellular functions, particularly in immune cells. The nuclear envelope may serve as a sensor/transducer of mechanical signals and an integrator of mechanical and chemical signals, allowing precise regulation of gene transcription and functionality in immune cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Oncology
Valentina Lodde, Rodrigo Garcia Barros, Laura Terzaghi, Federica Franciosi, Alberto Maria Luciano
Summary: This review highlights the emerging role of PGRMC1 in cell division, particularly in mitosis. Due to limited research on PGRMC1, there is still much to be explored regarding its function and mechanisms in cell division.
Article
Cell Biology
Kristina Mitic, Irene Meyer, Ralph Graef, Marianne Grafe
Summary: The study reveals that the permeabilization of the nuclear envelope in Dictyostelium discoideum occurs in synchrony with centrosome insertion and partial disassembly of nuclear pore complexes. Furthermore, it shows that restoration of nuclear envelope integrity usually happens after reassembly of NPCs and cytokinesis, accompanied by the concentration of ESCRT components at sites of nuclear envelope fenestration.
Article
Biochemistry & Molecular Biology
Diane Pelzer, Ludmilla de Plater, Peta Bradbury, Adrien Eichmuller, Anne Bourdais, Guillaume Halet, Jean-Leon Maitre
Summary: Cell fragmentation is a common phenomenon in human preimplantation embryos and is associated with poor prognosis during assisted reproductive technology procedures. This study uses light sheet microscopy imaging to reveal that inefficient chromosome separation due to spindle defects, caused by dysfunctional molecular motors Myo1c or dynein, leads to cell fragmentation during mitosis. The extended exposure of the cell cortex to chromosomes triggers actomyosin contractility and pinches off cell fragments, similar to the process observed during meiosis. This study uncovers the mechanisms underlying cell fragmentation in preimplantation embryos and provides insight into the regulation of mitosis during the maternal-zygotic transition.
Article
Cell Biology
Sherman Foo, Amaury Cazenave-Gassiot, Markus R. Wenk, Snezhana Oliferenko
Summary: The expansion of the nuclear envelope (NE) is crucial for maintaining nuclear shape and function during cell division. The depletion of diacylglycerol (DG) from the inner nuclear membrane and the rerouting of phosphatidic acid (PA) to membrane synthesis contribute to NE expansion. The conversion of DG to PA by diacylglycerol kinase Dgk1 and the synthesis of glycerophospholipids from DG through diacylglycerol cholinephosphotransferase/ ethanolaminephosphotransferase Ept1 play important roles in controlling NE expansion and mitotic fidelity.
JOURNAL OF CELL SCIENCE
(2023)
Review
Cell Biology
Nishit Srivastava, Guilherme Pedreira de Freitas Nader, Alice Williart, Romain Rollin, Damien Cuvelier, Alexis Lomakin, Matthieu Piel
Summary: The cell nucleus can adopt a variety of shapes in tissues due to cell deformities caused by cell crowding or migration through dense matrices. Recent studies have shown that the nuclear envelope can form blebs, leading to nuclear envelope opening and uncontrolled nucleocytoplasmic mixing. While blebs are a major source of nuclear instability, they are poorly understood and require more in-depth research.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yunan Ye, Hayden A. Homer
Summary: Previous studies have shown that microtubules play a role in nuclear centring during early embryonic divisions. In acentrosomal mouse zygotes, microtubules and actin work together to centre the pronuclei before the first mitosis. This study found that after the first mitotic division, the nuclei in acentrosomal embryos do not remain stationary in the cell centre but instead travel to the cortex in a microtubule-dependent manner. The high cytoplasmic viscosity in 2-cell embryos is associated with actin-dependent mechanisms for subsequent nuclear centring when microtubules exert a negative influence.
Article
Multidisciplinary Sciences
Marina Martinez-Garcia, Nadia Fernandez-Jimenez, Juan L. Santos, Monica Pradillo
SCIENTIFIC REPORTS
(2020)
Review
Plant Sciences
Nadia Fernandez-Jimenez, Monica Pradillo
JOURNAL OF EXPERIMENTAL BOTANY
(2020)
Editorial Material
Plant Sciences
Geraint Parry, Monica Pradillo, Aline V. Probst, Christophe Tatout
JOURNAL OF EXPERIMENTAL BOTANY
(2020)
Article
Multidisciplinary Sciences
Longfei Zhu, Nadia Fernandez-Jimenez, Maja Szymanska-Lejman, Alexandre Pele, Charles J. Underwood, Heidi Serra, Christophe Lambing, Julia Dluzewska, Tomasz Bieluszewski, Monica Pradillo, Ian R. Henderson, Piotr A. Ziolkowski
Summary: This study reveals the importance of the SMC5/6 complex in ensuring the proper progress of meiotic recombination in plants, showing the impact of SNI1 mutations and interactions with other genes on crossover distribution.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Christophe Tatout, Guillaume Mougeot, Geraint Parry, Celia Baroux, Monica Pradillo, David Evans
Summary: This paper introduces a range of resources developed by the INDEPTH COST Action, providing materials and tools for learning and researching epigenetics and nuclear structure. These resources aim to meet the demand for shared, high-quality resources and open access data repositories in the field of plant and crop science.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Fen Yang, Nadia Fernandez Jimenez, Joanna Majka, Monica Pradillo, Ales Pecinka
Summary: The study reveals that autotetraploid Arabidopsis plants with loss-of-function mutations in NSE2 and NSE4A subunits of the Structural Maintenance of Chromosomes 5/6 (SMC5/6) complex are almost sterile and produce hexaploid and aneuploid offspring. This suggests that the SMC5/6 complex plays a crucial role in maintaining tetraploid genome stability and that autotetraploid Arabidopsis plants have a higher tolerance for aneuploidy compared to diploids.
FRONTIERS IN PLANT SCIENCE
(2021)
Editorial Material
Plant Sciences
Olivier Da Ines, Kyuha Choi, Monica Pradillo, Christophe Lambing
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Francesco Blasio, Pilar Prieto, Monica Pradillo, Tomas Naranjo
Summary: Hybridization and polyploidy are important forces in plant adaptation and speciation. Interspecific gene flow generates adaptive genetic variants and increases yield in crops. The frequency of interspecific hybridization varies among taxa, with an estimated 9% occurrence. Allopolyploidy is more common than homoploid hybrid speciation. Chromosome doubling after hybridization is caused by cellular defects during meiosis. Unreduced gametes, formed at a frequency of 2.52% on average, result from altered spindle organization, disrupted kinetochore functioning, abnormal cytokinesis, or loss of meiotic division. Meiotic changes leading to cytological diploidization in allopolyploids are being understood, particularly in wheat. However, the understanding of homoeologous recombination suppressor genes in other allopolyploids is limited. The merger of two genomes leads to architectural, gene expression, and molecular interaction changes, resulting in phenotypic differences. This article provides an overview of genomic and transcriptomic changes during the early stages of allopolyploid formation.
Review
Cell Biology
Guillaume Mougeot, Tristan Dubos, Frederic Chausse, Emilie Pery, Katja Graumann, Christophe Tatout, David E. Evans, Sophie Desset
Summary: This review summarizes the main concepts and terminologies of deep learning in cell biology, emphasizing their availability and proposing best practices for sharing deep learning methods with biologists.
JOURNAL OF CELL SCIENCE
(2022)
Review
Agronomy
Serena Varotto, Tamar Krugman, Riccardo Aiese Cigliano, Khalil Kashkush, Ankica Kondic-Spika, Fillipos A. Aravanopoulos, Monica Pradillo, Federica Consiglio, Riccardo Aversano, Ales Pecinka, Dragana Miladinovic
Summary: Crop wild relatives (CWRs) possess epigenetic diversity that can contribute novel epialleles for crop improvement. Conservation and monitoring of epigenotypes can help preserve agrobiodiversity. Integrating epigenetics into breeding programs using advanced tools is a promising approach.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Plant Sciences
Pablo Parra-Nunez, Nadia Fernandez-Jimenez, Miguel Pachon-Penalba, Eugenio Sanchez-Moran, Monica Pradillo, Juan Luis Santos
Summary: Mutations affecting crossover frequency and distribution during meiosis can result in aneuploid gametes and sterility. The cytogenetic consequences of colchicine-induced autotetraploids from Arabidopsis mutants with altered crossover frequency were analyzed, revealing the potential of these mutants for studying key proteins in plant meiosis.
Article
Cell Biology
Nadia Fernandez-Jimenez, Marina Martinez-Garcia, Javier Varas, Felix Gil-Dones, Juan Luis Santos, Monica Pradillo
Summary: This study investigates the function of structural components of nuclear pore complexes (NPCs) in meiotic chromosome behavior. The study reveals that mutations in several nucleoporin genes lead to chromosome fragmentation and condensation abnormalities during meiosis. The findings provide important insights into the role of nucleoporins in meiotic chromosome behavior in plants.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Gaetan Pochon, Isabelle M. Henry, Chao Yang, Niels Lory, Nadia Fernandez-Jimenez, Franziska Boewer, Bingyan Hu, Lena Carstens, Helen T. Tsai, Monica Pradillo, Luca Comai, Arp Schnittger
Summary: ASY1 plays a crucial role in meiotic recombination by regulating the number and placement of cross-overs (COs). COs in asy1 mutants are positioned closer to each other, but the reduction in CO number is not significant. These findings contribute to our understanding of the mechanisms behind CO assurance and CO interference during meiosis.
Article
Biochemistry & Molecular Biology
Fen Yang, Nadia Fernandez-Jimenez, Martina Tuckova, Jan Vrana, Petr Capal, Mariana Diaz, Monica Pradillo, Ales Pecinka
Summary: Mutants of the Arabidopsis SMC5/6 complex subunits result in triploid offspring due to a meiotic defect, leading to the production of unreduced male gametes. The mutants show chromosome segregation absence and a disorganized microtubule network during meiotic division, with a high rate of ovule abortion observed. The SMC5/6 complex is partly required for repairing SPO11-induced DNA double-strand breaks, but the nonreduction phenomenon is SPO11-independent.
