Article
Biochemical Research Methods
Sandeep Kumar Kalva, Xose Luis Dean-Ben, Michael Reiss, Daniel Razansky
Summary: The authors propose spiral volumetric optoacoustic tomography (SVOT) for visualizing contrast agent perfusion and biodistribution in mice. The method overcomes the limitations of current whole-body imaging by providing optical contrast and unprecedented spatial and temporal resolution. SVOT allows visualization of deep-seated structures in living tissues and real-time imaging of biodynamics at the whole-organ level.
Article
Computer Science, Interdisciplinary Applications
Justine Robin, Ali Ozbek, Michael Reiss, Xose Luis Dean-Ben, Daniel Razansky
Summary: Spherical matrix arrays, combined with clinically-approved microbubble injection, enable multimodal imaging for non-invasive mapping of vascular networks and oxygenation in deep tissues. In experiments, this method achieved high spatial resolution and signal to noise ratio, while allowing for flow velocity mapping.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2022)
Article
Medicine, Research & Experimental
Berkan Lafci, Anna Hadjihambi, Madita Determann, Christos Konstantinou, Clara Freijo, Joaquin L. Herraiz, Sena Bluemel, Luc Pellerin, Neal C. Burton, Xose Luis Dean-Ben, Daniel Razansky
Summary: Non-alcoholic fatty liver disease (NAFLD) is a group of conditions associated with fat deposition and damage to liver tissue. Early detection of fat accumulation is crucial in preventing the progression of NAFLD to serious stages such as cirrhosis and hepatocellular carcinoma. The study used transmission-reflection optoacoustic ultrasound (TROPUS) to assess NAFLD in mice, providing spectroscopic differentiation of lipid content and mapping of speed of sound changes in lipid-rich regions.
Review
Biotechnology & Applied Microbiology
Xue-feng Shi, Bin Ji, Yanyan Kong, Yihui Guan, Ruiqing Ni
Summary: Optoacoustic imaging has versatile applications in biomedical research, visualizing disease pathophysiology and monitoring treatment effects in animal models, as well as in clinical settings. Multimodal imaging provides insights into the complex disease mechanisms with different molecular, structural, and functional readouts in vivo. Rapid development in molecular imaging contrast agents employing a multimodal imaging strategy for pathological targets involved in brain diseases is ongoing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Acoustics
Hector Estrada, Ali Ozbek, Justine Robin, Shy Shoham, Daniel Razansky
Summary: This article introduces a system for parallel optoacoustic imaging and transcranial ultrasound delivery with high resolution and wide field of view. The system is capable of generating and controlling focal spots with widths as small as 130 μm and capturing high-resolution 3D optoacoustic data in real time, showing potential for monitoring brain activity and effects of ultrasound emission in a non-invasive manner.
IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL
(2021)
Article
Cell Biology
Benedikt Hindelang, Teresa Nau, Ludwig Englert, Andrei Berezhnoi, Felix Lauffer, Ulf Darsow, Tilo Biedermann, Kilian Eyerich, Juan Aguirre, Vasilis Ntziachristos
Summary: The study found that optoacoustic mesoscopy offers a method for label-free assessment of inflammation biomarkers in human skin, providing potential guidance for disease and treatment characterization.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Review
Medicine, General & Internal
Jian Yang, Fengyan Zhang, Ying Qiao
Summary: Ultrasound (US), CT, and their combination have different diagnostic accuracies in detecting cervical lymph node metastasis (CLNM) in patients with papillary thyroid cancer (PTC). US has higher sensitivity and specificity than CT in detecting CLNM in patients with PTC, especially in the lateral compartment. The combination of US and CT increases the sensitivity in the patient-based analysis.
Article
Optics
Ruiqing Ni, Xose Luis Dean-Ben, Valerie Treyer, Anton Gietl, Christoph Hock, Jan Klohs, Roger M. Nitsch, Daniel Razansky
Summary: Imaging modalities, such as multi-spectral optoacoustic tomography (MSOT), have greatly advanced neurology and brain research. Non-invasive transcranial MSOT angiography has been demonstrated to visualize pial veins in the human brain, which can be beneficial for clinical brain imaging.
Article
Radiology, Nuclear Medicine & Medical Imaging
Lingli Xiao, Jianhua Zhou, Wen Tan, Ying Liu, Haoming Zheng, Guanying Wang, Wei Zheng, Xiaoqing Pei, Ankui Yang, Longzhong Liu
Summary: This study demonstrated that the postvascular phase of CEUS with perfluorobutane had excellent performance in diagnosing suspicious small lateral cervical LNs in patients with PTC. It showed improved diagnostic value compared to traditional ultrasonography alone. Overall, this study highlights the importance of utilizing contrast-enhanced US for better diagnosis of metastatic LNs in PTC.
Article
Engineering, Biomedical
Qiude Zhang, Quanquan Liu, Zhaohui Liu, Mingyue Ding, Ming Yuchi
Summary: The study investigates the impact of the distance between the central frequency and the transducer elements on the image resolution in full-aperture tomography. The results demonstrate that a shorter distance leads to higher resolution. The study also introduces the concept of spatial-domain pulse width broadening factor (SDPWBF) to evaluate the change in resolution. Numerical simulations and experiments corroborate the theoretical predictions.
PHYSICS IN MEDICINE AND BIOLOGY
(2023)
Article
Automation & Control Systems
Hao Liu, Chao Tan, Manuchehr Soleimani, Feng Dong
Summary: In this article, a novel transmission/reflection dual-mode image reconstruction algorithm based on information fusion is proposed. The transmissive attenuation and reflective time-delay information are integrated into an improved Lagrange framework to solve the objective function. The experimental results show that the proposed algorithm outperforms existing image fusion strategies in terms of accuracy and robustness.
IEEE TRANSACTIONS ON INDUSTRIAL INFORMATICS
(2023)
Article
Biochemical Research Methods
Mengyang Lu, Xin Liu, Chengcheng Liu, Boyi LI, Wenting Gu, Jiehui Jiang, Dean Ta
Summary: PA-GAN, a new image domain transformation method based on CycleGAN, is introduced to remove artifacts in PAT images caused by limited-view measurement data in an unsupervised manner. Experimental results demonstrate that PA-GAN performs well in removing artifacts in photoacoustic tomographic images without the need for ground-truth data.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Acoustics
Alex Benjamin, Gregory Ely, Brian W. Anthony
Summary: Quantitative ultrasound (QUS) and shear wave elastography (SWE) are important tools for diagnosing diseases, with SWE having limitations such as low-frame rates and sensitivity to operating conditions. Longitudinal speed of sound (SOS) is proposed as a potential alternative to SWE. A framework for obtaining 2D sound speed maps using a commercial ultrasound probe is validated and rigorously analyzed for uncertainty.
Article
Dermatology
Tassahil Messas, Achraf Messas, George Kroumpouzos
Summary: Optoacoustic imaging (OAI) is a hybrid imaging technique that combines optical contrast and ultrasound detection. Raster-scan optoacoustic mesoscopy (RSOM) is an emerging OAI method that provides information about the structure, function, and molecular features of dermatological conditions. Several studies have shown the usefulness of RSOM in inflammatory skin conditions such as psoriasis and eczema, particularly in quantifying changes to the dermal vasculature. RSOM can also aid in the early diagnosis and monitoring of disease activity and treatment efficacy in psoriasis. Multispectral RSOM, which uses multiple wavelengths of light, is beneficial in diagnosing and monitoring hypoxia-associated conditions such as systemic sclerosis and chronic wounds. OAI techniques can visualize the pathological vascularization of skin cancers, differentiate them from normal skin, measure the depth of malignant melanoma, and detect metastatic spread to lymph nodes. This review highlights the wide range of potential applications of OAI imaging, especially RSOM, in the diagnosis, treatment, and management of skin diseases.
CLINICS IN DERMATOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Zachary H. Levine, Bradley K. Alpert, Amber L. Dagel, Joseph W. Fowler, Edward S. Jimenez, Nathan Nakamura, Daniel S. Swetz, Paul Szypryt, Kyle R. Thompson, Joel N. Ullom
Summary: This article presents three-dimensional reconstructions of a region of an integrated circuit using x-ray computed tomography. The reconstructions are based on measurements obtained with a new high-magnification x-ray microscope. This innovative instrument generates x-rays in a 100 nm spot using a focused electron beam and employs energy-resolving x-ray detectors to minimize background noise and potentially identify materials within the sample. The reconstructions are consistent with the circuit's design file.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Computer Science, Interdisciplinary Applications
Ali Ozbek, Xose Luis Dean-Ben, Daniel Razansky
Summary: This paper presents a fast adaptive OAT data compression framework operating on fully sampled tomographic data. The framework is based on a wavelet packet transform that maximizes the data compression ratio according to the desired signal energy loss. A dedicated reconstruction method efficiently renders images directly from the compressed data.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2022)
Article
Acoustics
Berkan Lafci, Justine Robin, Xose Luis Dean-Ben, Daniel Razansky
Summary: Reflection ultrasound computed tomography (RUCT) is a new imaging technology that achieves optimal image quality for fully accessible objects from multiple directions. It effectively mitigates several deficiencies of conventional pulse-echo ultrasound systems. However, the small interelement pitch required in RUCT increases system complexity and costs, and limits imaging speed. This study explores acquisition schemes that enable RUCT imaging with a reduced number of transmit/receive elements and assesses the tradeoffs in achievable field of view, contrast-to-noise ratio, and temporal and spatial resolutions.
IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL
(2022)
Article
Optics
Ruiqing Ni, Xose Luis Dean-Ben, Valerie Treyer, Anton Gietl, Christoph Hock, Jan Klohs, Roger M. Nitsch, Daniel Razansky
Summary: Imaging modalities, such as multi-spectral optoacoustic tomography (MSOT), have greatly advanced neurology and brain research. Non-invasive transcranial MSOT angiography has been demonstrated to visualize pial veins in the human brain, which can be beneficial for clinical brain imaging.
Article
Engineering, Biomedical
Ahlam Said Mohamad Issa, Juergen Scheins, Lutz Tellmann, Alejandro Lopez-Montes, Joaquin L. Herraiz, Claudia Regio Brambilla, Hans Herzog, Irene Neuner, N. Jon Shah, Christoph Lerche
Summary: Dead time correction is crucial for accurate quantification in PET measurements. The currently used global dead time correction method may introduce quantification bias, while the block-pairwise dead time correction method significantly improves quantification accuracy.
PHYSICS IN MEDICINE AND BIOLOGY
(2022)
Article
Biochemical Research Methods
Wuwei Ren, Xose Luis Dean-ben, Zhiva Skachokova, Mark-Aurel Augath, Ruiqing Ni, Zhenyue Chen, Daniel Razansky
Summary: Progress in brain research relies on next-generation multi-modal imaging tools capable of capturing transient functional events and multiplexed contrasts noninvasively. This study presents a hybrid magnetic resonance and optoacoustic tomography system for murine brain imaging, enabling simultaneous dual-modality visualization of contrast agent dynamics. The system combines MR-compatible spherical matrix array transducer, fiber-based light illumination, and an optimized radiofrequency coil for whole-brain interrogation.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Chemistry, Multidisciplinary
Quanyu Zhou, Daniil Nozdriukhin, Zhenyue Chen, Lukas Glandorf, Urs A. T. Hofmann, Michael Reiss, Lin Tang, Xose Luis Dean-Ben, Daniel Razansky
Summary: This study introduces a new method for volumetric deep-tissue microangiography that overcomes the limitations of light diffusion and optical diffraction in wide-field imaging configurations. The proposed method combines stereovision and super-resolution localization imaging to achieve high-resolution 3D imaging and accurate blood flow quantification, enabling detailed visualization and analysis of microvascular networks.
Article
Chemistry, Multidisciplinary
Zhenyue Chen, Irmak Gezginer, Mark-Aurel Augath, Yu-Hang Liu, Ruiqing Ni, Xose Luis Dean-Ben, Daniel Razansky
Summary: This article reports on the development of a fully hybridized system enabling concurrent functional magnetic resonance optoacoustic tomography (MROT) measurements of stimulus-evoked brain-wide sensory responses in mice. The results indicate that the BOLD signals are highly correlated, both spatially and temporally, with the total hemoglobin readings resolved with volumetric multi-spectral optoacoustic tomography. Furthermore, the differential oxygenated and deoxygenated hemoglobin optoacoustic readings exhibit superior sensitivity as compared to the BOLD signals when detecting stimulus-evoked hemodynamic responses.
Article
Chemistry, Multidisciplinary
Joaquin L. Herraiz, Clara Freijo, Jorge Camacho, Mario Munoz, Ricardo Gonzalez, Rafael Alonso-Roca, Jorge Alvarez-Troncoso, Luis Matias Beltran-Romero, Maximo Bernabeu-Wittel, Rafael Blancas, Antonio Calvo-Cebrian, Ricardo Campo-Linares, Jaldun Chehayeb-Moran, Jose Chorda-Ribelles, Samuel Garcia-Rubio, Gonzalo Garcia-de-Casasola, Adriana Gil-Rodrigo, Cesar Henriquez-Camacho, Alba Hernandez-Piriz, Carlos Hernandez-Quiles, Rafael Llamas-Fuentes, Davide Luordo, Raquel Marin-Baselga, Maria Cristina Martinez-Diaz, Maria Mateos-Gonzalez, Manuel Mendez-Bailon, Francisco Miralles-Aguiar, Ramon Nogue, Marta Nogue, Borja Ortiz de Urbina-Antia, Alberto Angel Oviedo-Garcia, Jose M. Porcel, Santiago Rodriguez, Diego Anibal Rodriguez-Serrano, Talia Sainz, Ignacio Manuel Sanchez-Barrancos, Marta Torres-Arrese, Juan Torres-Macho, Angela Trueba Vicente, Tomas Villen-Villegas, Juan Jose Zafra-Sanchez, Yale Tung-Chen
Summary: This study aimed to investigate the inter-rater reliability (IRR) and performance of lung ultrasound (LUS) in detecting COVID-19 related signs in longitudinal or transverse orientation. Thirty-three physicians with advanced LUS experience independently evaluated ultrasound videos of 20 confirmed COVID-19 patients. The results showed substantial IRR for normal LUS, but only fair IRR for individual B-lines. No statistically significant differences were found between longitudinal and transverse scans. Standardized clinical protocols may improve the IRR of LUS in COVID-19 patients.
APPLIED SCIENCES-BASEL
(2023)
Article
Biochemical Research Methods
Sandeep Kumar Kalva, Xose Luis Dean-Ben, Michael Reiss, Daniel Razansky
Summary: The authors propose spiral volumetric optoacoustic tomography (SVOT) for visualizing contrast agent perfusion and biodistribution in mice. The method overcomes the limitations of current whole-body imaging by providing optical contrast and unprecedented spatial and temporal resolution. SVOT allows visualization of deep-seated structures in living tissues and real-time imaging of biodynamics at the whole-organ level.
Article
Multidisciplinary Sciences
Xose Luis Dean-Ben, Justine Robin, Daniil Nozdriukhin, Ruiqing Ni, Jim Zhao, Chaim Gluck, Jeanne Droux, Juan Sendon-Lago, Zhenyue Chen, Quanyu Zhou, Bruno Weber, Susanne Wegener, Anxo Vidal, Michael Arand, Mohamad El Amki, Daniel Razansky
Summary: By using dichloromethane microdroplets with high optical absorption, the authors demonstrate 3D microangiography of the mouse brain, overcoming the issue of strong background absorption from red blood cells.
NATURE COMMUNICATIONS
(2023)
Review
Chemistry, Multidisciplinary
Rebeca Tenajas, David Miraut, Carlos I. Illana, Rodrigo Alonso-Gonzalez, Fernando Arias-Valcayo, Joaquin L. Herraiz
Summary: Ultrasound is a versatile imaging modality widely used in medical examinations. Although it faces challenges in diagnostic performance due to manual operation and operator dependence, artificial intelligence has the potential to improve accuracy and repeatability by recognizing complex patterns and providing quantitative assessments. AI is being used in various areas of ultrasound scanning, including standard plane recognition, organ identification, extraction of clinical planes from 3D volumes, and guidance of acquisitions. However, standardized datasets and open-access repositories are needed to foster further development in this research field.
APPLIED SCIENCES-BASEL
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
F. Arias-Valcayo, P. Galve, Joaquin L. Herraiz, J. J. Vaquero, M. Desco, J. M. Udias
Summary: In this study, a spatially-variant resolution model was incorporated into a real time iterative reconstruction code for accurate imaging of multi-animal PET studies. The spatially variant point spread function (SV-PSF) was estimated using measurements and Monte Carlo simulations, and implemented in a GPU-based reconstruction code. The method showed uniform spatial resolution and superior image quality compared to the uniform PSF model.
BIOMEDICAL PHYSICS & ENGINEERING EXPRESS
(2023)
Article
Chemistry, Multidisciplinary
Fernando Arias-Valcayo, Pablo Galve, Jose Manuel Udias, Juan Jose Vaquero, Manuel Desco, Joaquin L. Herraiz
Summary: This study investigates the optimal conditions and motion compensation methods for preclinical PET studies using awake rats with attached point sources. Monte Carlo simulations and experimental validations are performed to determine the parameters for maximum detectability without compromising image quality.
APPLIED SCIENCES-BASEL
(2023)
Proceedings Paper
Acoustics
Cagla Ozsoy, Andrea Cossettini, Ali Ozbek, Sergei Vostrikov, Pascal Hager, Xose Luis Dean-Ben, Luca Benini, Daniel Razansky
Summary: The article introduces a compact, high-speed, low-cost, and configurable imager based on a novel custom-made optical-link software-defined ultrasound acquisition platform and a fiber-coupled laser diode illumination. The system has high imaging performance and can be widely used in neuroscience, dermatology, cancer, cardiovascular research, etc., and can achieve ultrafast in-vivo imaging.
PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2022
(2022)
Proceedings Paper
Acoustics
Neda Davoudi, Berkan Lafci, Ali Ozbek, Xose Luis Dean-Ben, Daniel Razansky
Summary: In this study, a convolutional neural network (CNN) approach was developed to enhance the quality of optoacoustic images by training on both time-resolved signals and tomographic reconstructions, overcoming distortions and artifacts caused by hardware design limitations. The combined methodology outperformed other CNN-based methods operating solely on image-domain data.
PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2022
(2022)
