Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 55, Issue 32, Pages 9365-9370Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201603813
Keywords
gas immobilization; hydrogen sulfide; imaging agents; nuclear imaging; radioisotopes
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Funding
- R&D Program of the National Research Foundation of Korea - Ministry of Science, ICT and Future Planning [2013M2A2A6042317, 20120006386, 2013R1A4A1069507]
- National Research Foundation of Korea [2013M2A2A6042317] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Grants-in-Aid for Scientific Research [16K15194] Funding Source: KAKEN
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Hydrogen sulfide (H2S) has multifunctional roles as a gas signaling molecule in living systems. However, the efficient detection and imaging of H2S in live animals is very challenging. Herein, we report the first radioisotope-based immobilization technique for the detection, quantification, and in vivo imaging of endogenous H2S. Macrocyclic Cu-64 complexes that instantly reacted with gaseous H2S to form insoluble (CuS)-Cu-64 in a highly sensitive and selective manner were prepared. The H2S concentration in biological samples was measured by a thin-layer radiochromatography method. When Cu-64-cyclen was injected into mice, an elevated H2S concentration in the inflamed paw was clearly visualized and quantified by Cerenkov luminescence and positron emission tomography (PET) imaging. PET imaging was also able to pinpoint increased H2S levels in a millimeter-sized infarcted lesion of the rat heart.
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