Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 55, Issue 40, Pages 12431-12435Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201606039
Keywords
immunoassays; isoelectric focusing; lab-on-a-chip; proteomics; single-cell analysis
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Funding
- UC Cancer Research Coordinating Committee
- University of California, Berkeley
- National Science Foundation
- National Science Foundation CAREER [CBET-1056035]
- National Institutes of Health [1R01CA203018]
- Directorate For Engineering [1056035] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys [1056035] Funding Source: National Science Foundation
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To measure protein isoforms in individual mammalian cells, we report single-cell resolution isoelectric focusing (scIEF) and high-selectivity immunoprobing. Microfluidic design and photoactivatable materials establish the tunable pH gradients required by IEF and precisely control the transport and handling of each 17-pL cell lysate during analysis. The scIEF assay resolves protein isoforms with resolution down to a single-charge unit, including both endogenous cytoplasmic and nuclear proteins from individual mammalian cells.
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