Article
Biochemistry & Molecular Biology
Mohammad Mousaei Ghasroldasht, Jin Seok, Hang-Soo Park, Farzana Begum Liakath Ali, Ayman Al-Hendy
Summary: Regenerative medicine is a promising therapeutic mode using stem cells for the treatment of challenging human diseases. Stem cells have pleotropic therapeutic potential through inflammation or apoptosis inhibition, cell recruitment, angiogenesis stimulation, and differentiation. This review provides an overview of the translation process from idea to clinical practice, including guidelines for clinical trial approval and a review of regenerative medicine clinical trials registered on the Clinicaltrials.gov website.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Talagavadi Channaiah Anudeep, Madhan Jeyaraman, Sathish Muthu, Ramya Lakshmi Rajendran, Prakash Gangadaran, Prabhu Chandra Mishra, Shilpa Sharma, Saurabh Kumar Jha, Byeong-Cheol Ahn
Summary: Alopecia, including scarring and non-scarring types, is a common diagnosis in clinical practice. The most prevalent type is non-scarring alopecia, with androgenetic alopecia and alopecia areata being the majority of cases. Traditional treatments have significant downsides, leading to the exploration of regenerative therapies. Growth factor-rich and stem cell-rich treatments have shown potential, but standardization is lacking. Further understanding and standardization of regenerative cellular therapies for hair restoration are needed.
Article
Ophthalmology
Michael Moller-Hansen, Ann-Cathrine Larsen, Peter Bjerre Toft, Charlotte Duch Lynggaard, Camillax Schwartz, Helle Bruunsgaard, Mandana Haack-Sorensen, Annette Ekblond, Jens Kastrup, Steffen Heegaard
Summary: The study evaluated the safety and feasibility of injecting allogeneic adipose-derived mesenchymal stem cells (ASCs) into the lacrimal gland (LG) for treating aqueous deficient dry eye disease (ADDE). Results showed that injection of ASCs into the LG was not associated with adverse events and led to improvements in ocular surface parameters. Further research is needed to confirm the therapeutic effect of allogeneic ASCs in a larger patient cohort.
Review
Medicine, Research & Experimental
Yingqian Zhu, Jianli Ge, Ce Huang, Hailiang Liu, Hua Jiang
Summary: Aging frailty is a complex geriatric syndrome characterized by disruption of physiological homeostasis and declining health status. Factors such as genome instability, DNA damage, and stem cell exhaustion contribute to the development of frailty with advancing age. Effective interventions are needed to improve the health status of frail patients.
Article
Agriculture, Dairy & Animal Science
Michela Mocchi, Elia Bari, Silvia Dotti, Riccardo Villa, Priscilla Berni, Virna Conti, Maurizio Del Bue, Gian Paolo Squassino, Lorena Segale, Roberto Ramoni, Maria Luisa Torre, Sara Perteghella, Stefano Grolli
Summary: The study demonstrates the safety and potential efficacy of canine mesenchymal stromal cells-secretome in treating osteoarthritis through both in vitro experiments and clinical trials on dogs.
Review
Cell & Tissue Engineering
Ria Margiana, Alexander Markov, Angelina O. Zekiy, Mohammed Ubaid Hamza, Khalid A. Al-Dabbagh, Sura Hasan Al-Zubaidi, Noora M. Hameed, Irshad Ahmad, R. Sivaraman, Hamzah H. Kzar, Moaed E. Al-Gazally, Yasser Fakri Mustafa, Homayoon Siahmansouri
Summary: MSCs have great potential for immunomodulation and tissue regeneration, and can be used for the treatment of various diseases. Recent advances in MSCs biology have facilitated their clinical application.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
W. Benton Swanson, Maiko Omi, Seth M. Woodbury, Lindsey M. Douglas, Miranda Eberle, Peter X. Ma, Nan E. Hatch, Yuji Mishina
Summary: This study investigated the impact of scaffold pore curvature on the differentiation trajectory of mesenchymal stem cells (MSCs), demonstrating that the curvature of scaffold pores can influence cell aggregation and differentiation by altering the cytoskeleton, ultimately guiding cell fate specification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Sichen Zhang, Dashuai Zhu, Zhenhua Li, Ke Huang, Shiqi Hu, Halle Lutz, Mengjie Xie, Xuan Mei, Junlang Li, Genevieve Neal-Perry, Shaowei Wang, Ke Cheng
Summary: The therapeutic ovarian regenerative patch (ORP) has been found to effectively treat POI and restore fertility in rats by stimulating the proliferation of ovarian somatic cells and inhibiting apoptosis under injury stress. This cell-free, off-the-shelf, and clinically feasible treatment represents a promising approach for POI.
Review
Biotechnology & Applied Microbiology
Zongjie Wang
Summary: Stem cells have the potential to proliferate and differentiate into various cell types, making them promising in regenerative medicine. However, concerns about their tumorigenicity require the assessment of safety in stem cell-derived therapies. Various approaches, including animal models, culture methods, and molecular techniques, can be used to assess tumorigenicity, each with its own advantages and limitations. Combining multiple assays may provide a more comprehensive evaluation. Future developments should focus on optimizing microfluidics-based methods and integrating multiple assays into a single platform for efficient and comprehensive tumorigenicity evaluation.
BIOENGINEERING-BASEL
(2023)
Article
Chemistry, Medicinal
Aleksandra Skubis-Sikora, Bartosz Sikora, Weronika Malysiak, Patrycja Wieczorek, Piotr Czekaj
Summary: Melatonin has been evaluated for its safety and biological activity on ADSCs in vitro, and it was found that low-dose melatonin can improve cell viability without negatively affecting osteogenic potential.
Article
Critical Care Medicine
Filip Rangatchew, Bo Sonnich Rasmussen, Jesper Dyrendom Svalgaard, Eva Haastrup, Maj-Lis M. Talman, Christian Bonde, Anne Fischer - Nielsen, Krzysztof T. Drzewiecki, Rikke Holmgaard, Lea Munthe - Fog
Summary: This study evaluated an automated multi-needle injection method for cutaneous MSC-therapy and demonstrated that the automated injector can deliver dermal cell-doses with acceptable cell quality.
Review
Cell Biology
Kosha J. Mehta
Summary: Mesenchymal stem cells (MSCs) play a valuable role in cell-based therapy for various pathological conditions, but iron-loading can hinder their functionality and impact both allogeneic and autologous MSC therapy. Understanding MSC biology from an iron perspective is clinically significant, highlighting the importance of iron regulation for MSC physiology in health and disease.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Agriculture, Dairy & Animal Science
Yanmin Wang, Michael Alexander, Todd Scott, Desiree C. T. Cox, Augusta Wellington, Mike K. S. Chan, Michelle B. F. Wong, Orn Adalsteinsson, Jonathan R. T. Lakey
Summary: Companion animals often suffer from ailments that cannot be treated by conventional veterinary medicine. Stem cell therapy, with its regenerative potential, offers a new approach for treating these ailments by promoting local tissue regeneration and repair. This review aims to summarize the various uses of stem cells in treating age-related and joint diseases in companion animals.
Article
Biochemistry & Molecular Biology
Ling Chen, Zhongwen Li, Wumei Wei, Bin An, Yao Tian, Wenjing Liu, Shuaishuai Niu, Yukai Wang, Liu Wang, Wei Li, Jie Hao, Jun Wu
Summary: Acute liver failure (ALF) is a clinical syndrome with high mortality. Liver transplantation is the only curative treatment for ALF, thus there is an urgent need for innovative therapies. This study evaluated the use of immunity-and-matrix regulatory cells (IMRCs) derived from human embryonic stem cells in treating ALF and investigated the underlying mechanism. The results showed that IMRCs improved liver histopathological changes, reduced liver enzyme levels in serum, promoted cell renewal, and protected against CCl4-induced liver damage.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Review
Orthopedics
Zetao Wang, Youguo Liao, Canlong Wang, Chenqi Tang, Cailian Fang, Junchao Luo, Hengzhi Liu, Xianan Mo, Zicheng Wang, Lingfang Shen, Junjuan Wang, Xiao Chen, Zi Yin, Jianyou Li, Weiliang Shen
Summary: Rotator cuff tendinopathy is a common disorder that causes a significant burden. Although stem cell therapy has shown promising results, there are many unresolved issues, such as determining the optimal source and dosage of stem cells, as well as standardizing patient types.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2023)
Article
Multidisciplinary Sciences
Satoshi Yasuda, Shinji Kusakawa, Takuya Kuroda, Takumi Miura, Keiko Tano, Nozomi Takada, Satoko Matsuyama, Akifumi Matsuyama, Michiyo Nasu, Akihiro Umezawa, Takao Hayakawa, Hideki Tsutsumi, Yoji Sato
Article
Multidisciplinary Sciences
Takuya Kuroda, Satoshi Yasuda, Hiroyuki Nakashima, Nozomi Takada, Satoko Matsuyama, Shinji Kusakawa, Akihiro Umezawa, Akifumi Matsuyama, Shin Kawamata, Yoji Sato
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Akiyuki Nishimura, Tsukasa Shimauchi, Tomohiro Tanaka, Kakeru Shimoda, Takashi Toyama, Naoyuki Kitajima, Tatsuya Ishikawa, Naoya Shindo, Takuro Numaga-Tomita, Satoshi Yasuda, Yoji Sato, Koichiro Kuwahara, Yoshito Kumagai, Takaaki Akaike, Tomomi Ide, Akio Ojida, Yasuo Mori, Motohiro Nishida
Article
Multidisciplinary Sciences
Emiko Ito, Shigeru Miyagawa, Maki Takeda, Ai Kawamura, Akima Harada, Hiroko Iseoka, Shin Yajima, Nagako Sougawa, Noriko Mochizuki-Oda, Satoshi Yasuda, Yoji Sato, Yoshiki Sawa
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Takuya Kuroda, Satoshi Yasuda, Shiori Tachi, Satoko Matsuyama, Shinji Kusakawa, Keiko Tano, Takumi Miura, Akifumi Matsuyama, Yoji Sato
NATURE COMMUNICATIONS
(2019)
Article
Cell & Tissue Engineering
Takeshi Watanabe, Satoshi Yasuda, Shinji Kusakawa, Takuya Kuroda, Mayumi Futamura, Mitsuhide Ogawa, Hidemi Mochizuki, Eri Kikkawa, Hatsue Furukawa, Masato Nagaoka, Yoji Sato
Summary: The MEASURE study aims to standardize the tumorigenicity evaluation of hPSC-derived CTPs, finding that the HEC assay and MACS can improve sensitivity to residual undifferentiated hPSCs. Multisite experiments confirmed the effectiveness of these methods in detecting low levels of undifferentiated cells.
Article
Multidisciplinary Sciences
Takamasa Hirai, Ken Kono, Rumi Sawada, Takuya Kuroda, Satoshi Yasuda, Satoko Matsuyama, Akifumi Matsuyama, Naoya Koizumi, Naoki Utoguchi, Hiroyuki Mizuguchi, Yoji Sato
Summary: The study demonstrates that the Ad vector is the most efficient in detecting undifferentiated hiPSCs in preparations of hiPSC-derived neural progenitor cells. It also shows that the Ad vector selectively eliminates hiPSC-NPCs, enabling sensitive detection of hiPSCs. This cytotoxic viral vector could enhance the quality and safety of hiPSCs-NPCs for therapeutic use.
SCIENTIFIC REPORTS
(2021)
Review
Cell & Tissue Engineering
Yoshiteru Kamiyama, Yoichi Naritomi, Yuu Moriya, Syunsuke Yamamoto, Tsukasa Kitahashi, Toshihiko Maekawa, Masahiro Yahata, Takeshi Hanada, Asako Uchiyama, Akari Noumaru, Yoshiyuki Koga, Tomoaki Higuchi, Masahiko Ito, Hiroyuki Komatsu, Sosuke Miyoshi, Sadaaki Kimura, Nobuhiro Umeda, Eriko Fujita, Naoko Tanaka, Taku Sugita, Satoru Takayama, Akihiko Kurogi, Satoshi Yasuda, Yoji Sato
Summary: Information on the biodistribution of cell therapy products is crucial for predicting and assessing their efficacy and toxicity. Understanding regulatory requirements, implementation status, and analytical methods is necessary for conducting BD studies. The results of this review will serve as a useful resource for implementing BD studies.
REGENERATIVE THERAPY
(2021)
Article
Cell & Tissue Engineering
Takako Yamamoto, Yoji Sato, Satoshi Yasuda, Masayuki Shikamura, Takashi Tamura, Chiemi Takenaka, Naoko Takasu, Masaki Nomura, Hiromi Dohi, Masayo Takahashi, Michiko Mandai, Yonehiro Kanemura, Masaya Nakamura, Hideyuki Okano, Shin Kawamata
Summary: This study suggests that genetic testing can predict abnormal tissue formation after transplantation of iPSC derivatives for cell therapy. While no positive correlation was found between the presence of SNVs/deletions and abnormal tissue formation, a copy number greater than 3 was strongly correlated. Additionally, CNV analysis may be useful in predicting abnormal tissue formation and reducing the need for tumorigenicity tests.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Yoshitaka Shirasago, Hidesuke Fukazawa, Shotaro Nagase, Yoshimi Shimizu, Tomoharu Mizukami, Takaji Wakita, Tetsuro Suzuki, Hideki Tani, Masuo Kondoh, Takuya Kuroda, Satoshi Yasuda, Yoji Sato, Kentaro Hanada, Masayoshi Fukasawa
Summary: The entry of the hepatitis C virus into host cells is a complex process mediated by various host factors. Through studying a highly infectious HCV-JFH1-tau substrain, researchers discovered that a single mutation in the E2 glycoprotein, M706L, was essential for infecting non-permissive cells. This mutant virus was able to use CLDN1, CLDN6, and CLDN9 to infect cells other than hepatocytes. Additionally, induction pluripotent stem cells were found to be susceptible to HCV infection, suggesting that HCV may change its receptor requirement through a single amino acid mutation to infect non-hepatic cells.
SCIENTIFIC REPORTS
(2022)
Article
Cell & Tissue Engineering
Takamasa Hirai, Ken Kono, Shinji Kusakawa, Satoshi Yasuda, Rumi Sawada, Akihiko Morishita, Shinko Hata, Atsushi Wakita, Takayasu Kageyama, Ryo Takahashi, Sono Watanabe, Norihiko Shiraishi, Yoji Sato
Summary: This study demonstrated the reproducibility of the cellular immortality test in detecting tumorigenic cellular impurities, as well as its ability to detect immortalized cells with different growth characteristics, contributing to the safety and quality of hCTPs.
REGENERATIVE THERAPY
(2022)
Article
Cell & Tissue Engineering
Takeshi Watanabe, Satoshi Yasuda, Connie L. Chen, Louise Delsing, Mick D. Fellows, Gabor Foldes, Shinji Kusakawa, Lucilia Pereira Mouries, Yoji Sato
Summary: The study evaluated the sensitivity and reproducibility of the highly efficient culture (HEC) assay for detecting residual undifferentiated human pluripotent stem cells (hPSCs) in cell therapy products. All facilities were able to detect spiked hiPSCs in hiPSC-derived cardiomyocytes, with repeatability being the main factor affecting reproducibility. The results show that the HEC assay is highly sensitive and can be widely used for tumorigenicity evaluation of hPSC-derived cell therapy products.
REGENERATIVE MEDICINE
(2023)
Article
Genetics & Heredity
Takumi Miura, Satoshi Yasuda, Yoji Sato
Summary: Next-generation sequencing (NGS) has revolutionized genetic/genomic research, particularly in detecting mutations associated with disease risk and evaluating cell substrates for biopharmaceuticals. This study developed a simple method to estimate the limit of detection (LOD) for low-frequency somatic mutations using whole-exome sequencing (WES) by NGS. Results showed that with 15 Gbp or more sequencing data, WES was able to detect mutations with allele frequencies between 5% and 10%, providing a rationale for setting a cutoff threshold in interpreting somatic genetic mutations.
Article
Multidisciplinary Sciences
Fumiya Ohashi, Shigeru Miyagawa, Satoshi Yasuda, Takumi Miura, Takuya Kuroda, Masayoshi Itoh, Hideya Kawaji, Emiko Ito, Shohei Yoshida, Atsuhiro Saito, Tadashi Sameshima, Jun Kawais, Yoshiki Sawa, Yoji Sato
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Ken Kono, Rumi Sawada, Takuya Kuroda, Satoshi Yasuda, Satoko Matsuyama, Akifumi Matsuyama, Hiroyuki Mizuguchi, Yoji Sato
SCIENTIFIC REPORTS
(2019)
Article
Biochemical Research Methods
Arnaud Marchant, Pierre Van Damme, Stanley Plotkin, Pieter Neels, Maria Cristina Cassetti, Jakob Cramer, Marion F. Gruber, David Goldblatt, Deborah King, Wendy Hartig-Merkel, Joris Vandeputte
Summary: A meeting was held in February 2023 by international organizations to discuss correlates of protection against COVID-19, reviewing evidence and identifying knowledge gaps. Neutralizing antibodies and T lymphocytes are believed to play a significant role in protection, but further studies are needed to consolidate the evidence and evaluate other potential factors.
Article
Biochemical Research Methods
Neetu Raghav, Seema Parveen, Simleen Kaur, Selwyn A. Wilson David, Hyesuk Kong, James L. Kenney, Rajesh K. Gupta
Summary: This study evaluated the use of solid medium in sterility testing. Results showed that the sterility test using Schaedler blood agar (SBA) was more sensitive and faster in detecting microorganisms compared to the current compendial method, particularly in samples containing thimerosal. The sterility test using solid medium could be completed in 7 days without any additional technology.
Article
Biochemical Research Methods
Loushambam Samananda Singh
Summary: Antibiotic resistance has become a major concern for global public health, and phage therapy is seen as an alternative for treating multidrug resistant bacterial infections. Nano-emulsion encapsulation is described as the best approach to maintain phage efficacy.
