Article
Pharmacology & Pharmacy
Xueping Zhang, Dan Deng, Daxin Cui, Yin Liu, Siyuan He, Hongmei Zhang, Yaorui Xie, Xiaoqian Yu, Shanshan Yang, Yulong Chen, Zhiguang Su
Summary: This study revealed a dual role of cholesterol sulfate (CS) in protecting pancreatic beta-cells and maintaining cell function, suggesting that CS might offer a physiological approach to preserve beta-cells and protect against the development of diabetes mellitus.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Yonela Ntamo, Ebrahim Samodien, Joleen Burger, Nolan Muller, Christo J. F. Muller, Nireshni Chellan
Summary: Immortalized rodent beta-cell lines have been traditionally studied in 2D culture techniques, but limitations in 2D culture have led to the development of 3D models. The study successfully demonstrated that the INS-1 3D spheroid model replicated in vivo tissue-like features, showing extended viability and potential for metabolic disease research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Suma Elumalai, Udayakumar Karunakaran, Jun-Sung Moon, Kyu-Chang Won
Summary: In type 2 diabetes, metabolic stress negatively affects pancreatic beta-cell function and survival. Imbalance in redox homeostasis causes abnormal tissue damage and beta-cell death, making beta-cells more susceptible to oxidative damage by NADPH oxidase.
Article
Food Science & Technology
Hikari Hirakida, Shinsuke Nakamura, Satoshi Inagaki, Shohei Tsuji, Masahiro Hayashi, Masamitsu Shimazawa, Hideaki Hara
Summary: This study evaluated the antidiabetic effect of an astaxanthin-rich extract (ARE) derived from Paracoccus carotinifaciens on pancreatic beta cells. The extract was found to increase insulin secretion and protect against cell death, potentially through the control of mitochondrial superoxide production.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biochemistry & Molecular Biology
Vivian Doerr, Ryan N. Montalvo, Branden L. Nguyen, Franccesco P. Boeno, Michael D. Sunshine, Victoria E. Bindi, David D. Fuller, Ashley J. Smuder
Summary: DOX chemotherapy can lead to cardiorespiratory dysfunction, while HBO therapy may increase muscle antioxidant expression. However, HBO therapy is not sufficient to prevent DOX-induced cardiorespiratory dysfunction.
Review
Biochemistry & Molecular Biology
Akinobu Nakamura
Summary: SGLT2 inhibitors not only lower blood glucose levels but also have protective effects on organs such as the heart and kidneys. These inhibitors prevent beta-cell dysfunction by reducing glucose overload, leading to increased beta-cell proliferation and decreased apoptosis. Clinical and preclinical data support the protective effect of SGLT2 inhibitors on beta-cell mass and function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Yue Sun, Ya-ping Bai, De-guo Wang, Yu-jie Xing, Teng Zhang, Wen Wang, Si-min Zhou, Jin-han Cheng, Wei-wei Chang, Xiang Kong, Xin-ming Yao, Li-qun Guo
Summary: Metformin treatment exerts a protective effect against beta-cell damage in patients with T2DM by modulating ferroptosis.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Endocrinology & Metabolism
Alexandra C. Title, Maria Karsai, Joan Mir-Coll, Oezlem Yavas Grining, Chantal Rufer, Sebastian Sonntag, Felix Forschler, Sayro Jawurek, Thomas Klein, Burcak Yesildag
Summary: The study introduces a novel method for assessing compound effects on beta-cell proliferation and count using reaggregated primary human islets, providing a representative 3D human islet model for high-throughput evaluation of short- and long-term changes in human beta-cell proliferation and function.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Mathematics, Applied
A. Ruiz-Silva
Summary: This paper discusses the emergence of collective behaviors in a network of a-cells coupled through gap-junction channels, presenting a pancreatic islet structure with different levels of connection and proposing a heterogeneous islet model with different dynamics in compact units. The conditions for achieving synchronization in the model and the emergence of cluster synchronization in the islet are established using Lyapunov stability theory. Numerical simulations are presented to illustrate the theoretical results.
PHYSICA D-NONLINEAR PHENOMENA
(2021)
Article
Materials Science, Multidisciplinary
Laura Clua-Ferre, Francesco De Chiara, Julia Rodriguez-Comas, Jordi Comelles, Elena Martinez, Amelie Luise Godeau, Ainhoa Garcia-Alaman, Rosa Gasa, Javier Ramon-Azcon
Summary: A novel bio-printing procedure is developed to fabricate large amounts of reproducible microspheres capable of secreting insulin. This method overcomes the limitations of traditional microsphere fabrication techniques and provides an efficient way to produce cell-laden microspheres.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Review
Biochemistry & Molecular Biology
Anna Weiser, Jerome N. Feige, Umberto De Marchi
Summary: Accumulation of calcium in energized mitochondria of pancreatic beta-cells plays a crucial role in beta-cell function. Mitochondrial calcium signaling is important for regulating insulin secretion and new pharmacological and genetic findings provide potential intervention strategies for modulating pancreatic beta-cell function in the context of diabetes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Zhen Zeng, Christoph Centner, Albert Gollhofer, Daniel Koenig
Summary: Whole dietary strategies rich in antioxidants have gained attention for their potential to alleviate exercise-induced oxidative stress, with most studies showing favorable effects. However, research on the effects of such strategies on oxidative stress is still limited, and further systematically designed studies are needed to strengthen the evidence.
Article
Pharmacology & Pharmacy
Yuya Mizukami, Yuki Takahashi, Kazunori Shimizu, Satoshi Konishi, Yoshinobu Takakura, Makiya Nishikawa
Summary: The study developed O-2-generating microwells by incorporating calcium peroxide (CaO2) into polydimethylsiloxane (PDMS)-based microwells, maintaining oxygen levels and improving cell viability in multicellular spheroids. This technique proved to be effective in preparing multicellular spheroids with high cell viability.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2021)
Review
Biochemistry & Molecular Biology
Petr Jezek, Blanka Holendova, Martin Jaburek, Jan Tauber, Andrea Dlaskova, Lydie Plecita-Hlavata
Summary: Pancreatic beta-cell insulin secretion is regulated by NADPH oxidase 4-mediated H2O2 production, requiring both ATP and H2O2 elevation to close the K-ATP channel. Additionally, simultaneous activation of other channels is necessary for the pulsatile exocytosis of insulin granule vesicles.
Article
Environmental Sciences
Siyu Zhang, Weiyan Hu, Mengran Li, Zhaoxia Guo, Liye Wang, Lihong Wu
Summary: The study reveals that there are supply-demand mismatches of multifunctional cultivated land in the Wuhan Metropolitan Area, with deficit regions of production function and ecological function decreasing in proportion at different scales, while deficit regions of landscape culture function remain concentrated in the center of the metropolitan area.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Article
Biotechnology & Applied Microbiology
Seiya Kanno, Yusuke Okubo, Tatsuto Kageyama, Lei Yan, Junji Fukuda
Summary: The number of man-made chemicals has increased rapidly in recent decades, and some of them may cause fetal malformations. However, animal testing cannot accurately predict human responses to chemicals. Therefore, researchers have proposed a signal reporter assay using human induced pluripotent stem cells (iPSCs) to detect potentially teratogenic chemicals.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Lei Yan, Tatsuto Kageyama, Binbin Zhang, Seiya Yamashita, Paul J. Molino, Gordon G. Wallace, Junji Fukuda
Summary: The study showed that electrical stimulation can activate the trichogenic gene expression in human dermal papilla cells, leading to increased hair growth, which could be beneficial for hair regenerative medicine.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Multidisciplinary Sciences
Seiya Kanno, Yusuke Okubo, Tatsuto Kageyama, Lei Yan, Satoshi Kitajima, Junji Fukuda
Summary: The number of man-made chemicals has been increasing rapidly in recent years, and some of these chemicals can cause fetal malformations. Disruption of complex and precisely programmed signaling pathways by external chemicals often leads to developmental toxicity. However, there is currently a lack of highly accurate and high-throughput screening assays for potential developmental toxicants. In this study, a reporter assay based on human-induced pluripotent stem cells (iPSCs) was proposed to detect changes in fibroblast growth factor signaling, which is crucial for limb morphogenesis. This assay successfully predicted the capacity of chemicals to induce limb malformations.
Article
Biotechnology & Applied Microbiology
Monami Yamane, Jieun Seo, Yinghui Zhou, Tomoki Asaba, Shan Tu, Ayaka Nanmo, Tatsuto Kageyama, Junji Fukuda
Summary: Transcriptome comparisons of dermal papilla cells (DPCs) in hair beads (HB) culture and typical 2D culture revealed the upregulation of the phosphoinositide 3-kinase and Akt (PI3K/Akt) signaling pathway in HB culture. Inhibition of this pathway decreased the hair-inducing capability of DPCs in HBs, while activation of the pathway improved trichogenous gene expression of DPCs in 2D culture.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Engineering, Biomedical
Ayaka Nanmo, Lei Yan, Tomoki Asaba, Licheng Wan, Tatsuto Kageyama, Junji Fukuda
Summary: This study proposes a scalable and automated approach for the preparation of highly hair-inductive grafts using a bioprinter. The method utilizes collagen droplets to facilitate hair follicle regeneration through cell interactions. This approach holds great potential for clinical applications.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Yuka Sawada, Hanae Tsukumo, Junji Fukuda, Kazutoshi Iijima, Hiroshi Itagaki
Summary: To improve the accuracy of predicting chemical skin sensitization using cell-based alternative methods, researchers developed a skin sensitization test system using THP-1 cells as a substitute for dendritic cells and keratinized normal human epidermal keratinocytes (NHEK). The expression of CD54 and CD86 on THP-1 cells was measured after exposure to chemicals via keratinized NHEK, and it was found that the composition of the medium has a significant effect on the evaluation index in the co-culture system.
APPLIED SCIENCES-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Tatsuto Kageyama, Riki Anakama, Hideru Togashi, Junji Fukuda
Summary: This study investigated the effects of cell sorting manipulation on hair regeneration in vitro using Y27632. The supplementation of Y27632 modulated the spatial distribution of cells and promoted the development of hair follicle organoids. The study also revealed the involvement of specific signaling pathways in the process.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Engineering, Biomedical
Hyeonyu Kim, Tatsuya Osaki, Roger D. Kamm, H. Harry Asada
Summary: Creating a vasculature in engineered human skeletal muscle tissues has various advantages, including increasing tissue thickness, improving cell survival, providing models for drug testing, and enhancing muscle differentiation. The researchers demonstrated the creation of contractile skeletal muscle tissues with a central vascular channel and microvascular networks. By recreating the in vivo extracellular fluid compartments, they were able to construct large vascularized skeletal muscle tissues for drug testing platforms. Coculturing with incompatible media from an early stage of muscle differentiation led to improved muscle differentiation and contractile force.
Article
Multidisciplinary Sciences
Tatsuto Kageyama, Akihiro Shimizu, Riki Anakama, Rikuma Nakajima, Kohei Suzuki, Yusuke Okubo, Junji Fukuda
Summary: This study demonstrates the successful induction of hair follicle formation in vitro by controlling the interactions between epithelial and mesenchymal cells. The in vitro hair follicle model has potential applications in understanding hair follicle induction, evaluating drug effects on hair growth and inhibition, and modeling gray hairs.
Article
Materials Science, Biomaterials
Sugi Hirano, Tatsuto Kageyama, Maki Yamanouchi, Lei Yan, Kohei Suzuki, Katsumi Ebisawa, Keiichiro Kasai, Junji Fukuda
Summary: Hair regeneration using HFSCs and dermal papilla cells has potential for alopecia treatment. This study developed a novel approach for HFSC expansion through cell aggregates formation and Matrigel encapsulation. The HFSCs cultured using a microarray device showed a 20-fold increase in quantity and higher expression of trichogenic stem cell markers compared to conventional culture methods. HFSCs cultured using this method combined with embryonic mesenchymal cells demonstrated vigorous hair regeneration in nude mice. Moreover, the expansion of human HFSCs from androgenetic alopecia patients showed significant improvement using this approach. Therefore, this study proposes a useful method for HFSC expansion in hair regenerative medicine.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Mio Aoki, Ryoto Yokota, Shoji Maruo, Tatsuto Kageyama, Junji Fukuda
Summary: This study investigated the efficacy of slow vitrification methods for freezing hair follicle germs (HFGs) and proposed a microfinger device to scale up the cryopreservation process. The results showed that DMSO vitrification yielded efficient hair regeneration, comparable to non-cryoprotected HFGs, and the microfinger device achieved over 50% hair regeneration rate for cryopreserved HFGs.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2023)
Article
Multidisciplinary Sciences
Do-Won Jeong, Jong-Wan Park, Kyeong Seog Kim, Jiyoung Kim, June Huh, Jieun Seo, Ye Lee Kim, Joo-Youn Cho, Kwang-Woong Lee, Junji Fukuda, Yang-Sook Chun
Summary: This study demonstrates that palmitic acid (PA) acts as a molecular checkpoint of lipid reprogramming in liver cancer cells. PA mediates the palmitoylation of PHF2, leading to ubiquitin-dependent degradation of PHF2 and reducing lipogenesis. Additionally, PHF2 functions as an E3 ubiquitin ligase and destabilizes the master transcription factor of lipogenesis, SREBP1c. The induction of PA triggers the PHF2/SREBP1c axis, which increases lipogenesis in hepatocellular carcinoma (HCC). Therefore, monitoring dietary PA levels may be important for HCC patients.
NATURE COMMUNICATIONS
(2023)