Article
Multidisciplinary Sciences
Xuexiang Lin, Xiao-Yu Liu, Bo Zhang, Ai-Qing Qin, Kwok-Min Hui, Kevin Shi, Yang Liu, Don Gabriel, X. James Li
Summary: A virus enzyme assay using a luciferin derivative as the substrate has been developed as an alternative method for detection of acute viral infection, particularly for influenza diagnosis. The assay showed similar detection rates for all influenza types and subtypes except for A(H7N9) due to lower viral titer. When tested with diluted specimens in comparison to a real-time polymerase chain reaction assay, the assay demonstrated high sensitivity and specificity.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, General & Internal
Huijun Zong, Shengwei Zhang, Xueyi Shang, Hua Jiang, Zhongpeng Zhao, Shaolong Chen, Xin Wang, Ye Wang, Yongqiang Jiang, Xinyu Li, Lingyun Tan, Peng Liu, Qingyu Lv, Yan Li
Summary: After optimizing the conditions, AlphaLISA showed higher sensitivity and throughput in the detection of influenza B virus, and can be used for virus diagnosis and epidemic control.
FRONTIERS IN MEDICINE
(2023)
Article
Environmental Sciences
Warish Ahmed, Aaron Bivins, Mikayla Stephens, Suzanne Metcalfe, Wendy J. M. Smith, Kwanrawee Sirikanchana, Masaaki Kitajima, Stuart L. Simpson
Summary: This study analyzed wastewater samples from four wastewater treatment plants in Queensland, Australia, and found the presence of 13 respiratory viruses. The results were compared with clinical cases, showing the potential of wastewater surveillance in viral disease monitoring. The most commonly detected viruses were bocavirus, parechovirus, rhinovirus A, and rhinovirus B.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Virology
Jahan Ara Begum, Ismail Hossain, Mohammed Nooruzzaman, Jacqueline King, Emdadul Haque Chowdhury, Timm C. Harder, Rokshana Parvin
Summary: This study assessed the pathogenicity of low-pathogenic avian influenza (LPAI) H9N2 viruses in commercial poultry in Bangladesh. The results showed that these viruses caused mild to moderate tracheitis, pneumonia, and enteritis in Sonali and commercial broiler chickens. The study also found that the viruses predominantly replicated in the respiratory tract and were shed through the oropharyngeal route.
Article
Chemistry, Analytical
Junmin Li, Xuejiao Hu, Xiaoming Wang, Jianing Yang, Lei Zhang, Qianyun Deng, Xiqin Zhang, Zixia Wang, Tieying Hou, Shan Li
Summary: A novel one-tube rapid diagnostic approach based on formamide and colorimetric RT-LAMP was established for the detection of SARS-CoV-2 without the extraction of specific viral RNA. The technique showed high specificity and could visually detect the virus within 45 minutes.
ANALYTICA CHIMICA ACTA
(2021)
Article
Infectious Diseases
Masato Inaba, Yuki Higashimoto, Yoko Toyama, Tomoya Horiguchi, Masaya Hibino, Mitsunaga Iwata, Kazuyoshi Imaizumi, Yohei Doi
Summary: The study demonstrates that RT-LAMP has a diagnostic accuracy comparable to RT-qPCR in the early phase of COVID-19, until the 9th day after symptom onset. However, after the 10th day, the positivity rate of RT-LAMP significantly decreases, suggesting its limitations in diagnosing COVID-19 in later stages of the disease.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Immunology
Rebecca L. Smith, Laura L. Gibson, Pamela P. Martinez, Ruian Ke, Agha Mirza, Madison Conte, Nicholas Gallagher, Abigail Conte, Leyi Wang, Richard Fredrickson, Darci C. Edmonson, Melinda E. Baughman, Karen K. Chiu, Hannah Choi, Tor W. Jensen, Kevin R. Scardina, Shannon Bradley, Stacy L. Gloss, Crystal Reinhart, Jagadeesh Yedetore, Alyssa N. Owens, John Broach, Bruce Barton, Peter Lazar, Darcy Henness, Todd Young, Alastair Dunnett, Matthew L. Robinson, Heba H. Mostafa, Andrew Pekosz, Yukari C. Manabe, William J. Heetderks, David D. McManus, Christopher B. Brooke
Summary: This study found that RT-qPCR tests are more effective than antigen tests at identifying individuals infected with SARS-CoV-2 prior to or early during the infectious period. Additionally, serial testing multiple times per week can increase the sensitivity of antigen tests.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Chemistry, Analytical
Muhabat Adeola Raji, Yumna Aloraij, Fatimah Alhamlan, Ghadeer Suaifan, Karina Weber, Dana Cialla-May, Juergen Popp, Mohammed Zourob
Summary: This study developed a novel, low-cost and easy-to-use colorimetric flu virus biosensor assay. The assay utilizes a sandwich format with antibodies immobilized on cotton swabs for rapid detection of flu A and B viruses.
Article
Engineering, Environmental
Xiawan Zheng, Keyue Zhao, Xiaoqing Xu, Yu Deng, Kathy Leung, Joseph T. Wu, Gabriel M. Leung, Malik Peiris, Leo L. M. Poon, Tong Zhang
Summary: Wastewater surveillance is an effective tool for controlling infectious diseases, providing cost-effective and real-time monitoring results. In this study, we developed preanalytical protocols for influenza virus surveillance in wastewater and conducted large-scale monitoring in Hong Kong. The concentration of influenza viruses in wastewater was associated with clinical detection rates and illness rates in the community, and increased significantly after the mask mandate was cancelled.
Review
Environmental Sciences
Vu Duc Canh, Miaomiao Liu, Jatuwat Sangsanont, Hiroyuki Katayama
Summary: Waterborne diseases caused by pathogenic human viruses are a major public health concern. An advanced approach called capsid integrity (RT-)qPCR has been developed to assess the infectivity of pathogenic viruses. This study reviews the development and application of this approach and discusses its efficiency and potential future applications.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Akihiko Hata, Yuno Meuchi, Miaomiao Liu, Shotaro Torii, Hiroyuki Katayama
Summary: Polymerase chain reaction (PCR) is widely used for monitoring pathogenic viruses in water environments. This study characterized and validated methods for quantifying active viruses and indicators in surface water. The study found that CDDP-RT-qPCR can be used for quantifying virus concentrations with higher detection frequencies than culture assays, but it may overestimate the activity of the virus.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
Katarzyna Linkowska, Tomasz Bogiel, Katarzyna Lamperska, Andrzej Marszalek, Jaroslaw Starzynski, Lukasz Szylberg, Aleksandra Szwed-Kowalska, Malgorzata Pawlowska, Tomasz Grzybowski
Summary: Although SARS CoV-2 infection may not be as serious a threat as before, the increased transmissibility of the Omicron variant remains a challenge for health care systems. This study evaluates the performance of three SARS-CoV-2 diagnostic tests and finds that all have high specificity and sensitivity for detecting the virus. The LOD values for each test were determined and suggest that the reported outcomes should be based on internal validation rather than manufacturer's declarations.
SCIENTIFIC REPORTS
(2023)
Article
Veterinary Sciences
Yushun Wang, Mincai Nie, Huidan Deng, Siyuan Lai, Yuancheng Zhou, Xiangan Sun, Ling Zhu, Zhiwen Xu
Summary: In this study, a rapid and specific diagnostic method for porcine rotavirus type A (PoRVA) was successfully developed. The method utilized specific primers and probes designed based on the PoRVA VP6 gene, and obtained detection results that were completely consistent with the TaqMan probe-based RT-qPCR method in clinical samples.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Harry H. Jenkins, Ana A. Tellechea Lopez, Francesco Saverio Tarantini, Hannah Tomlin, Danielle Scales, I-Ning Lee, Siyu Wu, Ralph Hyde, Katarzyna Lis-Slimak, Timothy Byaruhanga, Jamie L. Thompson, Sara Pijuan-Galito, Lara Doolan, Kazuyo Kaneko, Penny Gwynne, Caroline Reffin, Emily Park, Jayasree Dey, Jack Hill, Asta Arendt-Tranholm, Amy Stroud, Moira Petrie, Chris Denning, Andrew Benest, Claire Seedhouse
Summary: Polymerase chain reaction (PCR) has been proven to be the gold-standard for SARS-CoV-2 detection. Performing a non-invasive one-step RT-qPCR assay directly on saliva specimens simplifies sample collection, reduces processing time and costs. The assay demonstrates good sensitivity and concordance.
SCIENTIFIC REPORTS
(2022)
Article
Infectious Diseases
Byron Freire-Paspuel, Miguel Angel Garcia-Bereguiain
Summary: A new triplex RT-qPCR assay based on CDC primers and probes showed 100% specificity and 97.7% sensitivity, with a limit of detection at 1000 copies/mL. This assay could provide a reliable and efficient method for SARS-CoV-2 diagnosis, especially in times of testing supply shortages.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)