Letter
Hematology
Yoko Nishinaka-Arai, Akira Niwa, Shiori Matsuo, Yasuhiro Kazuki, Yuwna Yakura, Takehiko Hiroma, Tsutomu Toki, Tetsushi Sakuma, Takashi Yamamoto, Etsuro Ito, Mitsuo Oshimura, Tatsutoshi Nakahata, Megumu K. Saito
Article
Developmental Biology
Alice Pieplow, Meseret Dastaw, Tetsushi Sakuma, Naoaki Sakamoto, Takashi Yamamoto, Mamiko Yajima, Nathalie Oulhen, Gary M. Wessel
Summary: Researchers are exploring the manipulation of gene function through CRISPR-Cas9 editing of cis-regulatory sequences, aiming to minimize the side effects of typical mutations in coding regions. This approach allows for reducing gene activity without causing lethality from complete gene knockouts, and is also effective in rapidly identifying key regulatory sites essential for gene expression. The study further demonstrated this strategy by targeting established genomic cis-regulatory regions and utilizing a deadCas9-VP64 transcriptional activator to modulate gene transcription directly. Additionally, a traditional GFP reporter construct approach was paired with CRISPR-Cas9 to investigate the transcriptional regulation of a key gene in the sea urchin, providing insights into the potential post-transcriptional mechanisms behind its germ cell identity.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Kenshi Watanabe, Charose Marie Ting Perez, Tomoki Kitahori, Kosuke Hata, Masato Aoi, Hirokazu Takahashi, Tetsushi Sakuma, Yoshiko Okamura, Yutaka Nakashimada, Takashi Yamamoto, Keisuke Matsuyama, Shinzo Mayuzumi, Tsunehiro Aki
Summary: Efficiency of specific gene knock-in by homologous recombination was significantly increased by over 10 times through combining the CRISPR-Cas9 system in Aurantiochytrium strains. Genome edited strains with higher fatty acid productivity were isolated by disrupting genes associated with beta-oxidation, demonstrating the effectiveness of CRISPR-Cas9 for molecular breeding in Aurantiochytrium species to improve lipid productivity.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Endocrinology & Metabolism
Ji Min Lee, Ukjin Kim, Hyokyung Yang, Bokyeong Ryu, Jin Kim, Tetsushi Sakuma, Takashi Yamamoto, Jae-Hak Park
Summary: Recent advancements in gene editing using TALENs have facilitated the creation of genetically engineered animal models. The Nkx3.1 knockout rat model was successfully generated, revealing reduced fertility, decreased prostate weights, increased epithelial cell layers, elevated mRNA expression of genes linked to prostate carcinogenesis, and a propensity for malignant breast tumors. This model serves as a valuable tool for studying the impact of NKX3.1 deficiency on prostate health, fertility, and breast cancer development.
Article
Cell Biology
Kaichi Watanabe, Yuhei Yasui, Yuta Kurose, Masashi Fujii, Takashi Yamamoto, Naoaki Sakamoto, Akinori Awazu
Summary: This study investigated the process of gastrulation in sea urchin embryos and found that omeprazole may induce partial exogastrulation by disrupting the apical-basal polarity of F-actin distribution.
Article
Gastroenterology & Hepatology
Tomoki Takashina, Akihiro Matsunaga, Yukiko Shimizu, Tetsushi Sakuma, Tadashi Okamura, Kunie Matsuoka, Takashi Yamamoto, Yukihito Ishizaka
Summary: A protein-based artificial transcription system was developed to engineer hepatocyte-like cells from human umbilical cord-derived mesenchymal stem cells. The engineered cells exhibited functional and molecular characteristics similar to hepatocytes, and significantly improved the survival rate in mice with hepatic failure. This transcription system could serve as a versatile tool for cell therapy for hepatic failures.
HEPATOLOGY COMMUNICATIONS
(2023)
Meeting Abstract
Chemistry, Applied
Kenshi Watanabe, Charose Marie Ting Perez, Tomoki Kitahori, Kosuke Hata, Masato Aoi, Hirokazu Takahashi, Tetsushi Sakuma, Yoshiko Okamura, Yutaka Nakashimada, Takashi Yamamoto, Shinzo Mayuzumi, Tsunehiro Aki
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
(2021)
Article
Endocrinology & Metabolism
Yuichiro Arima, Yoshiko Nakagawa, Toru Takeo, Toshifumi Ishida, Toshihiro Yamada, Shinjiro Hino, Mitsuyoshi Nakao, Sanshiro Hanada, Terumasa Umemoto, Toshio Suda, Tetsushi Sakuma, Takashi Yamamoto, Takehisa Watanabe, Katsuya Nagaoka, Yasuhito Tanaka, Yumiko K. Kawamura, Kazuo Tonami, Hiroki Kurihara, Yoshifumi Sato, Kazuya Yamagata, Taishi Nakamura, Satoshi Araki, Eiichiro Yamamoto, Yasuhiro Izumiya, Kenji Sakamoto, Koichi Kaikita, Kenichi Matsushita, Koichi Nishiyama, Naomi Nakagata, Kenichi Tsujita
Summary: Ketogenesis plays a protective role in mitochondrial function during the perinatal period, critical for survival of neonates and infants.
Meeting Abstract
Oncology
Takashi Yamamoto, Tetsushi Sakuma
Meeting Abstract
Oncology
Tetsushi Sakuma
Meeting Abstract
Oncology
Tetsushi Sakuma
Meeting Abstract
Oncology
Kazuma Nakatani, Tetsushi Sakuma, Takashi Yamamoto, Yoshitaka Hippo, Yusuke Suenaga
Meeting Abstract
Oncology
Yukihito Ishizaka, Yoichi Teratake, Tetsushi Sakuma, Takashi Yamamoto
Meeting Abstract
Oncology
Takuya Fukazawa, Etsuko Yokota, Tetsushi Sakuma, Yoshio Naomoto, Takashi Yamamoto, Tomoki Yamatsuji