Article
Endocrinology & Metabolism
Hyo Jeong Kim, Ka-Young Ryu, Yong-Gun Kim, Myoung Ok Kim, Ji Hye Lee, Min-Kyoung Song, Young-Jin Youn, Nitin Kumar Pohkrel, Sung-Hyun Kim, Jae-Young Kim, Hye-Jin Jung, Woo-Shin Kim, Chang-Won Hong, Hong-Hee Kim, Youngkyun Lee
Summary: The study revealed the crucial role of PTP1B in osteoclast differentiation, with PTP1B deficiency leading to decreased osteoclast number and activity. This highlights the potential of PTP1B as a therapeutic target for bone-lytic diseases.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Genetics & Heredity
Xu Cheng, Xiaoting Song, Zhiyan Li, Chiting Yuan, Xinhuan Lei, Mingxuan Feng, Zhenghua Hong, Liwei Zhang, Dun Hong
Summary: Hormonal homeostasis is crucial for bone remodeling. Treating intestinal inflammation can regulate bone resorption in distant bones. Increased intestinal permeability may lead to systemic inflammation and bone loss. LPS could be a key mediator in the gut-bone axis. AOAH participates in bone metabolism mainly through inhibiting osteoclast differentiation.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2022)
Article
Dentistry, Oral Surgery & Medicine
Shuang Liu, Xijiao Yu, Qiushuang Guo, Shuaiqi Zhao, Kaixian Yan, Meng Hou, Fuxiang Bai, Shu Li
Summary: This study revealed that the knockout of PLAP-1 inhibits osteoclast differentiation and decreases alveolar bone resorption through the TGF-beta 1/Smad1 signaling pathway, which could serve as an innovative target for the prevention and treatment of periodontitis.
JOURNAL OF PERIODONTOLOGY
(2023)
Article
Cell Biology
Xinyue Liang, Yafei Hou, Lijuan Han, Shuxiang Yu, Yunyun Zhang, Xiumei Cao, Jianshe Yan
Summary: Bone homeostasis is maintained by a balance between osteoblast-mediated bone formation and osteoclast-mediated bone resorption. ELMO1, as a regulator of Rac1, plays a crucial role in osteoclast differentiation and bone resorption, with Elmo1(-/-) mice exhibiting defective bone resorption and alleviated bone erosion in a rheumatoid arthritis model.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Noreen Mumtaz, Marijke Koedam, Johannes P. T. M. van Leeuwen, Marion P. G. Koopmans, Bram C. J. van der Eerden, Barry Rockx
Summary: Bone-related complications are common following arbovirus infection, including Zika virus (ZIKV). This study investigated the effect of ZIKV infection on osteoclastogenesis and found that infection reduced osteoclast formation and bone resorption. Additionally, antibody-dependent enhancement may play a role in ZIKV-associated bone pathology.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Muhanad Ali, Nathan Kucko, John A. Jansen, Fang Yang, X. Frank Walboomers
Summary: The study showed that PDLCs stimulated by LPS can significantly increase osteoclastogenesis and enhance specific gene expression, while treatment with LXA4 exhibits strong anti-inflammatory activity and can reverse these effects. This suggests that PDLCs may be a potential target for treating bacterial-induced bone resorption in patients with periodontal disease, and LXA4 is a promising candidate for such therapy.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Dentistry, Oral Surgery & Medicine
T. M. Taira, E. S. Ramos-Junior, P. H. Melo, C. C. Costa-Silva, M. G. Alteen, D. J. Vocadlo, W. B. Dias, F. Q. Cunha, J. C. Alves-Filho, K. Soe, S. Y. Fukada
Summary: Osteoclasts are important in bone mass regulation and have high metabolic activity. The study shows that metabolic reprogramming towards the hexosamine biosynthetic pathway (HBP) is necessary for osteoclast differentiation and determining bone resorption modes. In addition, O-GlcNAcylation facilitates aggressive trench resorption in human cells.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Medicine, Research & Experimental
Seong Cheol Kim, Hyeon Jeong Kim, Gi Eun Park, Ramesh Prasad Pandey, Jisun Lee, Jae Kyung Sohng, Yong Il Park
Summary: This study demonstrated that Tri inhibits osteoclastic cell differentiation and bone resorption by down-regulating the expression of osteoclastogenic marker genes and signalling proteins, bone resorptive proteins, and by augmenting serum OPG/RANKL ratio, suggesting that Tri can be a novel anti-osteoporotic compound for treating senile and postmenopausal OP.
Review
Immunology
Tatjana Bordukalo-Niksic, Vera Kufner, Slobodan Vukicevic
Summary: Bone remodeling in the adult skeleton involves a continuous process of old and damaged bone removal by osteoclasts and subsequent new bone formation by osteoblasts. BMPs play a crucial role in maintaining healthy bone, affecting both bone formation and resorption, and may be involved in pathological processes related to inflammatory disorders affecting bones and joints.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Hailong Wang, Jinqiao Li, Zihan Xu, Feng Wu, Hongyu Zhang, Chao Yang, Jian Chen, Bai Ding, Xiukun Sui, Zhifeng Guo, Yinghui Li, Zhongquan Dai
Summary: Our study demonstrated that ucOCN negatively affected the early differentiation and bone resorption of osteoclasts through the Gprc6a receptor. The inhibitory effects of ucOCN on osteoclasts were mainly observed at the early stage of osteoclastogenesis. Signaling analysis suggested that Gprc6a receptor played a crucial role in mediating the effects of ucOCN.
Article
Dentistry, Oral Surgery & Medicine
Saki Nakane, Kentaro Imamura, Rio Hisanaga, Kazuyuki Ishihara, Atsushi Saito
Summary: The study demonstrated that CTLA-4-Ig can effectively inhibit bone resorption in periodontitis by affecting osteoclast differentiation and activity, potentially through the regulation of the NF-kappa B pathway and PP2A expression.
JOURNAL OF PERIODONTAL RESEARCH
(2021)
Article
Genetics & Heredity
Salman M. Toor, Sachin Wani, Omar M. E. Albagha
Summary: Osteoclasts are the only bone resorbing cells and play an important role in skeletal mass and structure. Differentiation of osteoclasts involves a complex regulatory network of genes and LncRNAs, with potential implications for understanding bone-related diseases.
FRONTIERS IN GENETICS
(2021)
Article
Medicine, Research & Experimental
Biao Wu, Jie Shang, Shiyuan Lin, Ning Jiang, Baizhou Xing, Rong Peng, Xianghe Xu, Huading Lu
Summary: Increased bone resorption caused by excessive osteoclasts is the main cause of osteoporosis. The expression of RILP, a protein, is induced by receptor activator of NF-KB ligand, and its inhibition decreases the number and function of osteoclasts. Inhibition of RILP also reduces preosteoclast migration and bone resorption. The study suggests that RILP plays a crucial role in osteoclast formation and bone resorption, and could be a therapeutic target for bone diseases caused by excessive osteoclasts.
LABORATORY INVESTIGATION
(2023)
Article
Endocrinology & Metabolism
Samuel D. Burciaga, Flavia Saavedra, Lori Fischer, Karen Johnstone, Eric D. Jensen
Summary: Studies using kinase inhibitors have found that PRKD is necessary for the formation and function of osteoclasts. However, the role of individual PRKD genes and their significance in skeletal dynamics is still unclear. In this study, we show that PRKD3 is the primary form expressed in osteoclasts. Knockout of Prkd3 reduces osteoclast differentiation and resorptive function, leading to increased bone mass in male mice. Protection against bone loss was not observed in a periodontal disease model. Overall, our data demonstrate that PRKD3 promotes osteoclastogenesis both in vitro and in vivo.
Article
Biochemistry & Molecular Biology
Perry C. Caviness, Dongzheng Gai, Oxana P. Lazarenko, Michael L. Blackburn, Fenghuang Zhan, Jin-Ran Chen
Summary: Osteoclasts derived from hematopoietic stem cells play a crucial role in bone resorption. Identifying new molecules that can epigenetically regulate osteoclastogenesis is essential for the development of novel treatments for bone deterioration disorders like osteoporosis. The protein Ezh2 has been found to be associated with the epigenetic regulation of various cellular processes, but its role in bone cell development is still not fully understood.
Article
Biochemistry & Molecular Biology
Ryo Tanaka, Shosei Yoshinouchi, Kento Karouji, Yuki Tanaka, Tsukasa Tominari, Michiko Hirata, Chiho Matsumoto, Yoshifumi Itoh, Chisato Miyaura, Masaki Inada
Summary: A novel intranasal injection model of lung cancer was developed for anticancer drug screening, simulating the native lung cancer pathology. The model proved to be a powerful tool for investigating lung cancer pathology and evaluating the efficacy of drugs.
Article
Biochemistry & Molecular Biology
Shosei Yoshinouchi, Kento Karouji, Tsukasa Tominari, Moe Sugasaki, Chiho Matsumoto, Chisato Miyaura, Michiko Hirata, Yoshifumi Itoh, Masaki Inada
Summary: Prostate cancer highly metastasizes to bone and is associated with severe bone resorption and bone formation. In this study, the researchers investigated the role of membrane-bound TGF-a in prostate cancer-induced bone formation through autocrine PGE2 signaling in osteoblasts. The results suggest that TGF-a enhances ERK signaling while also inducing PGE2-mediated bone formation, indicating that prostate cancer regulates both PGE2-mediated bone resorption and bone formation at the site of bone metastasis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Physiology
Noriaki Kawanishi, Takaki Tominaga, Katsuhiko Suzuki
Summary: This study found that resistance exercise, specifically electrical pulse stimulation-induced muscle contraction, resulted in changes in the miRNA and mRNA profiles of circulating extracellular vesicles. Muscle-specific miRNAs such as miR-1, miR-133, and miR-206 showed significant upregulation after 90 minutes of muscle contraction. Additionally, mRNA expression changes in circulating extracellular vesicles after muscle contraction were found to promote angiogenesis and regulate the immune response. These changes in extracellular vesicle properties may play a crucial role in resistance exercise-induced physiological adaptations.
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY, INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Fangfei Qin, Boyuan Li, Hui Wang, Sihui Ma, Jiaofeng Li, Shanglin Liu, Linghao Kong, Huangtao Zheng, Rongfeng Zhu, Yu Han, Mingdong Yang, Kai Li, Xiong Ji, Peng R. Chen
Summary: We developed a strategy named SiTomics for systematic mapping of dynamic modifications and subsequent profiling of chromatinized proteome and genome defined by specific chromatin acylations. Our SiTomics toolkit revealed distinct crotonylation and β-hydroxybutyrylation upon short chain fatty acids stimulation and established linkages for chromatin acylation mark-defined proteome, genome, and functions. SiTomics offers a plat-form technology for elucidating the metabolites-modification-regulation axis, which is widely applicable for multi-omics profiling and functional dissection of modifications beyond acylations and proteins beyond histones.
Article
Biochemistry & Molecular Biology
Takaki Tominaga, Jiapeng Huang, Shuo Wang, Miwa Noguchi, Yishan Tong, Momoko Asano-Oritani, Katsuhiko Suzuki
Summary: Several food components, including polyphenols and phytonutrients, have immunomodulatory effects. Collagen, known for its various bioactivities, can promote wound healing and relieve symptoms of bone/joint disease. This study investigated the immunomodulatory effects of collagen-derived dipeptides and amino acids on macrophages and peripheral blood mononuclear cells (PBMC), and found that there is no difference in cytokine secretion between dipeptides and amino acids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Shiori Wakasugi-Onogi, Sihui Ma, Ruheea Taskin Ruhee, Yishan Tong, Yasuhiro Seki, Katsuhiko Suzuki
Summary: This study found that sulforaphane has various effects on neutrophil functions, including inhibiting reactive oxygen species (ROS) production, degranulation, phagocytosis, and neutrophil extracellular trap (NET) formation. Sulforaphane also has direct antioxidant activity. It was observed that sulforaphane can decrease the release of inflammatory cytokines and myeloperoxidase from neutrophils. In addition, sulforaphane directly removes ROS, including hypochlorous acid (HOCl).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Michiko Hirata, Tsukasa Tominari, Ryota Ichimaru, Naruhiko Takiguchi, Yuki Tanaka, Masaru Takatoya, Daichi Arai, Shosei Yoshinouchi, Chisato Miyaura, Chiho Matsumoto, Sihui Ma, Katsuhiko Suzuki, Florian M. W. Grundler, Masaki Inada
Summary: In this study, the demethylated forms of citrus nobiletin and tangeretin, 4'-demethylnobiletin and 4'-demethyltangeretin, were found to inhibit osteoclast differentiation and function via the suppression of the NF-KB pathway. These compounds may have potential applications in maintaining bone health and preventing metabolic bone diseases such as osteoporosis.
Article
Nutrition & Dietetics
Chika Ando, Sihui Ma, Moe Miyoshi, Kyohei Furukawa, Xuguang Li, Huijuan Jia, Hisanori Kato
Summary: Maternal malnutrition affects offspring health by manipulating the epigenome, but recent studies suggest that postnatal nutrition can reverse DNA methylation changes. This study used DNA methylation arrays to examine the methylation status of renal promoter regions and the effects of postnatal protein intake. The findings showed that protein intake in the offspring reprogrammed the methylation status of 2,395 DNA regions and reset 34 genes. These genes, including Adora2b, Trpc5, Ar, Xrcc2, and Atp1b1, are involved in renal disease and blood pressure regulation. The study highlights the potential of postnatal nutritional interventions in targeting epigenetic changes for hypertension prevention and treatment.
FRONTIERS IN NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Hiroaki Sako, Mohieldin Youssef, Olga Elisseeva, Takayuki Akimoto, Katsuhiko Suzuki, Takashi Ushida, Tadashi Yamamoto
Summary: Slower translation rates reduce protein misfolding by allowing time for proper folding. MicroRNAs (miRNAs), which are absent in prokaryotes, can stall translating ribosomes, preventing misfolding with little effect on protein abundance. This eukaryote-specific mechanism provides insights into co-translational protein folding and has potential in targeting protein misfolding diseases.
Review
Nutrition & Dietetics
Cong Wu, Katsuhiko Suzuki
Summary: Flavonoids may have a great potential to treat sarcopenia by improving skeletal muscle mass, muscle strength, and physical performance in older adults.
Article
Biochemistry & Molecular Biology
Sihui Ma, Sae Takasugi, Masayoshi Sugawara, Kenji Saito, Huijuan Jia, Hisanori Kato
Summary: This study evaluated the effects of heat-moisture-treated high-amylose rice on body weight, lipid metabolism, and gut microbiome composition in a rat model of obesity. Heat-moisture treatment, rather than the level of amylose content, contributes to the observed anti-obesity and cholesterol-lowering effects. The study also identified candidate genes contributing to the cholesterol-regulating potential and demonstrated the influence of heat-moisture-treated rice flour on gut microbiome, particularly the Ruminococcus taxa.
Article
Medicine, General & Internal
Mahsa Naghashpour, Dian Dayer, Hadi Karami, Mahshid Naghashpour, Mahin Taheri Moghadam, Seyed Mohammad Jafar Haeri, Katsuhiko Suzuki
Summary: This study demonstrates that magnolol has a significant anti-tumor effect on MKN-45 cells and can be used to overcome cisplatin resistance in gastric cancer cells when combined with cisplatin.
MEDICINA-LITHUANIA
(2023)