Article
Chemistry, Multidisciplinary
Shota Hozumi, Satoko Honda, Takayuki Arie, Seiji Akita, Kuniharu Takei
Summary: Continuous multiple data health monitoring has the potential to detect abnormal conditions or early stages of diseases. A promising architecture for monitoring small vital signals is an attachable flexible multimodal sensor system that can detect multiple health conditions from the skin surface. While recent breakthroughs have enabled continuous monitoring of sweat chemicals or physical conditions using flexible sensors, integrating multimodal sensors to simultaneously and accurately monitor chemical and physical information remains a challenge.
Article
Environmental Sciences
Kevin T. Le, Zhouyuan Yuan, Areeb Syed, Devin Ratelle, Eric C. Orenstein, Melissa L. Carter, Sarah Strang, Kasia M. Kenitz, Pedro Morgado, Peter J. S. Franks, Nuno Vasconcelos, Jules S. Jaffe
Summary: By using deep learning methods, we were able to effectively classify and identify marine plankton using images collected by in situ microscopes. The accuracy of our method was evaluated and compared to established measurement methods, showing good consistency and correlation.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Chemistry, Analytical
Lei Guo, Jinyu Zhu, Keqi Wang, Kian-Kai Cheng, Jingjing Xu, Liheng Dong, Xiangnan Xu, Can Chen, Mudassir Shah, Zhangxiao Peng, Jianing Wang, Zongwei Cai, Jiyang Dong
Summary: In this study, a deep learning model called DeepFERE was proposed to fuse multimodal images and enhance the spatial resolution of mass spectrometry imaging (MSI). Results showed that the DeepFERE model was able to generate high-resolution reconstruction images with rich chemical information and detailed structure, and improve the delimitation of cancerous and para-cancerous regions.
ANALYTICAL CHEMISTRY
(2023)
Review
Medicine, General & Internal
Yu Luo, Guangcan Xu, Hongyu Li, Tianju Ma, Zi Ye, Zhaohui Li
Summary: The fluidics system in phacoemulsification plays a crucial role. Both the gravity-fluidics and active-fluidics systems are commonly used, with the active-fluidics system having advantages in maintaining stable intraocular pressure. However, its low penetration rate and high cost hinder its wider usage. The article provides an overview of the active-fluidics system's mechanism and performance, aiming to guide future research.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Optics
Luis E. Villegas-Hernandez, Vishesh Dubey, Mona Nystad, Jean-Claude Tinguely, David A. Coucheron, Firehun T. Dullo, Anish Priyadarshi, Sebastian Acuna, Azeem Ahmad, Jose M. Mateos, Gery Barmettler, Urs Ziegler, Asa Birna Birgisdottir, Aud-Malin Karlsson Hovd, Kristin Andreassen Fenton, Ganesh Acharya, Krishna Agarwal, Balpreet Singh Ahluwalia
Summary: This study introduces a photonic chip as a high-throughput microscopy platform for super-resolution imaging of histological samples. It demonstrates diverse imaging capabilities and paves the way for the adoption of super-resolution high-throughput multimodal analysis of cryopreserved tissue samples.
LIGHT-SCIENCE & APPLICATIONS
(2022)
Article
Optics
M. S. Pochechuev, A. A. Lanin, I. Kelmanson, A. S. Chebotarev, E. S. Fetisova, D. S. Bilan, E. K. Shevchenko, A. A. Ivanov, A. B. Fedotov, V. V. Belousov, A. M. Zheltikov
Summary: Multimodal nonlinear microscopy combining third-harmonic generation (THG) with two- and three-photon excited fluorescence (2PEF and 3PEF) provides a powerful resource for high-fidelity imaging of nucleoli and nucleolar proteins. With a suitably tailored genetically encoded fluorescent stain, reliable detection of specific nucleolar proteins' 2PEF/3PEF readout against extranucleolar signal enables high-contrast imaging. THG offers a versatile readout for imaging unstained nucleoli in various biological systems, from neurons in brain slices to cultured HeLa cells.
Article
Chemistry, Multidisciplinary
[Anonymous]
Summary: The vortex fluidic device accelerated immunoblot assay (VAIA) is a new method that significantly reduces the processing time of immunoblot from nearly 2 hours to less than 5 minutes. Gregory A. Weiss and his colleagues demonstrate the wide applicability and potential of VAIA in their Communication.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Emily C. Sanders, Sanjana R. Sen, Aidan A. Gelston, Alicia M. Santos, Xuan Luo, Keertna Bhuvan, Derek Y. Tang, Colin L. Raston, Gregory A. Weiss
Summary: Unlocking the potential of personalized medicine in point-of-care settings requires the development of a new generation of biomarker and proteomic assays. A newly developed immunoassay method shows efficient, robust, and cost-effective characteristics, making it applicable in both laboratory and clinical settings.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Physics, Particles & Fields
Andrew Lifson, Malin Sjodahl, Zenny Wettersten
Summary: This paper introduces a new method, called chirality-flow formalism, which simplifies Feynman rules and diagrams and speeds up the numerical evaluation of scattering amplitudes. Experimental results show that using this method reduces computation time and improves efficiency.
EUROPEAN PHYSICAL JOURNAL C
(2022)
Article
Chemistry, Physical
Chih-Feng Wang, Patrick Z. El-Khoury
Summary: The study reveals that nonlinear optical processes can be enhanced at plasmonic tip-sample nanojunctions, allowing for high-spatial-resolution chemical analysis through enhanced spectroscopies.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Irene Wacker, Ronald Curticean, Daniel Ryklin, Britta Weidinger, Frederik Mayer, Li-Yu Huang, Julian Hoffmann, Monsur Islam, Nadine von Coelln, Tanja Schmitt, Christian Huck, Petra Tegeder, Florian Feist, Jochen A. Kammerer, Christopher Barner-Kowollik, Martin Wegener, Eva Blasco, Ulrich Gengenbach, Rasmus R. Schroder
Summary: With the rapid progress in additive manufacturing, there is a demand for nanoscale characterization of micro-patterned heterostructures of soft materials. Advanced function-structure relationships can be achieved through direct 3D structuring, which requires morphological and spectroscopical imaging at nanometer resolution. This article demonstrates the use of serial ultramicrotomy to access the interior of 3D objects and highlights the power of correlative imaging and spectroscopy techniques for understanding emerging functions from nanoscopic structuring of carbon-based materials in 3D.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Physics, Applied
Guan-Yu Zhuo, K. U. Spandana, K. M. Sindhoora, Yury Kistenev, Fu-Jen Kao, Viktor V. Nikolaev, Hale Zuhayri, Natelya A. Krivova, Nirmal Mazumder
Summary: This paper discusses the application of a compact MNLO microscope in clinical research for label-free non-invasive molecular imaging, providing insights into molecular properties and disease progression in biological systems and organs. The integration of nonlinear optical phenomena with multidimensional imaging allows for addressing complex biomedical questions.
JOURNAL OF APPLIED PHYSICS
(2021)
Article
Chemistry, Multidisciplinary
Violaine Hubert, Ines Hristovska, Szilvia Karpati, Sarah Benkeder, Arindam Dey, Chloe Dumot, Camille Amaz, Naura Chounlamountri, Chantal Watrin, Jean-Christophe Comte, Fabien Chauveau, Emmanuel Brun, Patrice Marche, Frederic Lerouge, Stephane Parola, Yves Berthezene, Thomas Vorup-Jensen, Olivier Pascual, Marlene Wiart
Summary: This study proposes and validates a preclinical in vivo MRI tool to monitor neuroinflammation following ischemic stroke, based on injection of a novel multimodal nanoprobe designed for internalization by phagocytic cells. The study confirms efficient internalization of NanoGd by microglia and shows hypointense signals in the ischemic lesion using in vivo MRI with NanoGd injection in a mouse model. Additionally, the study demonstrates NanoGd internalization by activated phagocytic cells through both intravital microscopy and ex vivo analysis.
Article
Multidisciplinary Sciences
Yu-Chung Lin, Elena Perevedentseva, Zhe-Rui Lin, Chia-Chi Chang, Hsiang-Hsin Chen, Shun-Min Yang, Ming-Der Lin, Artashes Karmenyan, Giorgio Speranza, Luca Minati, Christoph Nebel, Chia-Liang Cheng
Summary: Hybrid core-shell nanodiamond-gold nanoparticles with tunable and tailored properties have been synthesized, providing new options for optical labeling, imaging, sensing, drug delivery, and targeted treatment in biomedical applications. The combination of nanostructured gold and nanodiamond properties offers various bioimaging modalities and potential theranostic functionalities.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Artem S. Chebotarev, Veronika S. Ledyaeva, Olga I. Patsap, Anatoli A. Ivanov, Andrei B. Fedotov, Vsevolod V. Belousov, Arina G. Shokhina, Aleksandr A. Lanin
Summary: Label-free imaging of genetically induced hepatocellular carcinoma in a murine model using two- and three-photon fluorescence microscopy provides valuable information about cell arrangement, lipid accumulation, fibrogenesis, and steatosis, demonstrating the potential of multimodal label-free microscopy for visualizing liver pathology development.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Multidisciplinary Sciences
Lena Harker-Kirschneck, Anne E. Hafner, Tina Yao, Christian Vanhille-Campos, Xiuyun Jiang, Andre Pulschen, Fredrik Hurtig, Dawid Hryniuk, Sian Culley, Ricardo Henriques, Buzz Baum, Andela Saric
Summary: This study developed a physical model for the division of archaeal cells, which share similarities with eukaryotes. By comparing simulations with live cell imaging experiments, the researchers proposed a previously unidentified division mechanism based on changes in the curvature of elastic cytoskeletal filaments. This study not only reveals this mechanism in archaea, but also suggests its potential application in other biological systems.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Materials Science, Biomaterials
Mario Del Rosario, Hannah S. Heil, Afonso Mendes, Vittorio Saggiomo, Ricardo Henriques
Summary: The maker movement has brought 3D printing technology into optics labs, revolutionizing the field by improving accessibility and allowing rapid, low-cost prototyping. This approach fosters collaboration among researchers and promotes democratization of technology, extending the open-access concept from knowledge to technology.
Review
Biotechnology & Applied Microbiology
Etienne Boulter, Julien Colombelli, Ricardo Henriques, Chloe C. Feral
Summary: LEGO (R) is not just a toy for children, but also a building ecosystem that can be used for designing and assembling various devices for scientific research and biotechnology. This emerging trend in LEGO (R) building pairs with a growing interest in open-access and frugal science.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Immunology
David Tomaz, Pedro Matos Pereira, Nadia Guerra, Julian Dyson, Keith Gould, Ricardo Henriques
Summary: NK cell inhibition and activation rely on the nanoscale colocalization of their receptors. NKG2D and Ly49A integrate signals at the immune synapse based on their relative positioning, which is affected by the size of NK cell ligand-receptor pairs.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Cell Biology
Christophe Leterrier, Pramod A. Pullarkat
Summary: This article reviews the mechanical properties of spectrins, their organization in red blood cells and neurons, and their contribution to the mechanical properties of these cells.
JOURNAL OF CELL SCIENCE
(2022)
Editorial Material
Multidisciplinary Sciences
Guillaume Charras, Martin Lenz
Article
Multidisciplinary Sciences
Manasi Kelkar, Pierre Bohec, Matthew B. Smith, Varun Sreenivasan, Ana Lisica, Leo Valon, Emma Ferber, Buzz Baum, Guillaume Salbreux, Guillaume Charras
Summary: Proper orientation of the mitotic spindle is essential for embryo development, tissue development, and maintenance of tissue integrity and homeostasis in adults. This study used optogenetics and computational modeling to investigate how mitotic spindles respond to inhomogeneous tension within the actomyosin cortex. The results showed that the activation of RhoA at both poles of the cell influences spindle orientation by reducing pulling forces exerted by cortical regulators on astral microtubules. This perturbation of torque balance leads to spindle rotation in response to mechanical stress.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Afonso Mendes, Hannah S. Heil, Simao Coelho, Christophe Leterrier, Ricardo Henriques
Summary: Understanding the structure of supramolecular complexes is important for studying their function and disease modulation. Super-resolution microscopy combined with single-particle analysis overcomes technical limitations and provides detailed structural information, broadening our knowledge.
Letter
Biochemical Research Methods
Wei Ouyang, Jiachuan Bai, Manish Kumar Singh, Christophe Leterrier, Paul Barthelemy, Samuel F. H. Barnett, Teresa Klein, Markus Sauer, Pakorn Kanchanawong, Nicolas Bourg, Mickael M. Cohen, Benoit Lelandais, Christophe Zimmer
Article
Biochemistry & Molecular Biology
Morgan Gazzola, Alexandre Schaeffer, Ciaran Butler-Hallissey, Karoline Friedl, Benoit Vianay, Jeremie Gaillard, Christophe Leterrier, Laurent Blanchoin, Manuel Thery
Summary: Microtubule self-repair was investigated by microinjecting purified tubulin dimers labeled with a red fluorophore into cells expressing GFP-tubulin. The appearance of red dots along the green microtubules indicated the incorporation of injected dimers into damaged sites, replacing the pre-existing green dimers. The location of these repair sites was found to be more concentrated in regions with a less dense actin filament network and greater lateral fluctuations of microtubules.
Article
Cell Biology
Joanna W. Pylvanainen, Romain F. Laine, Bruno M. S. Saraiva, Sujan Ghimire, Gautier Follain, Ricardo Henriques, Guillaume Jacquemet
Summary: Fast4DReg is a new tool developed for ImageJ or Fiji that can quickly correct axial and lateral drift in 3D video-microscopy datasets. It outperforms other state-of-the-art open-source drift-correction tools in terms of speed and accuracy, and can be used to register misaligned channels in 3D.
JOURNAL OF CELL SCIENCE
(2023)
Article
Chemistry, Analytical
Liliana J. Gomes, Joao P. Carrilho, Pedro M. Pereira, Artur J. Moro
Summary: This study presents a new Near InfraRed (NIR) fluorescent chemosensor that can detect metal ions and anions. The sensor is based on a synthetic analogue of the natural anthocyanin family and has a metal chelating unit. It shows high sensitivity to Zn2+ and has selectivity towards ATP and ADP. The sensor also exhibits strong luminescence in bacterial and mammalian cells, suggesting potential applications in intracellular labeling.
Article
Biochemistry & Molecular Biology
Helena Veiga, Ambre Jousselin, Simon Schaper, Bruno M. Saraiva, Leonor B. Marques, Patricia Reed, Joana Wilton, Pedro M. Pereira, Sergio R. Filipe, Mariana G. Pinho
Summary: Unregulated cell cycle progression in bacteria can have lethal consequences. A new study has revealed a link between chromosome replication/segregation and division septum splitting. The protein FtsK, which contains a DNA translocase domain, has been found to regulate the cellular levels of a peptidoglycan hydrolase Sle1. This regulation is crucial for cell separation in the bacterial pathogen Staphylococcus aureus.
Article
Chemistry, Medicinal
John R. Schultz, Stephen K. Costa, Gorakhnath R. Jachak, Pooja Hegde, Matthew Zimmerman, Yan Pan, Michaele Josten, Chinedu Ejeh, Travis Hammerstad, Hans Georg Sahl, Pedro M. Pereira, Mariana G. Pinho, Veronique Dartois, Ambrose Cheung, Courtney C. Aldrich
Summary: Nosocomial infections caused by resistant Gram-positive organisms are increasing, and new drugs are urgently needed. This study describes the identification of a 5-amino-4-quinolone with potent Gram-positive activity and preliminary understanding of its mechanism of action and resistance. The compound shows promising antibacterial properties and warrants further investigation for the treatment of Gram-positive infections.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
John R. Schultz, Stephen K. Costa, Gorakhnath R. Jachak, Pooja Hegde, Matthew Zimmerman, Yan Pan, Michaele Josten, Chinedu Ejeh, Travis Hammerstad, Hans Georg Sahl, Pedro M. Pereira, Mariana G. Pinho, Veronique Dartois, Ambrose Cheung, Courtney C. Aldrich
Summary: This article describes the discovery of a new compound, 5-amino-4-quinolone 111, which shows exceptional activity against multidrug-resistant Gram-positive bacteria with low minimum inhibitory concentrations. Preliminary studies suggest that this compound selectively disrupts bacterial membranes, does not induce resistance, and has a high therapeutic index.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
