Article
Multidisciplinary Sciences
Amir Mirbeik, Robin Ashinoff, Tannya Jong, Allison Aued, Negar Tavassolian
Summary: This study evaluates the application of a real-time high-resolution millimeter-wave imaging system for in-vivo skin cancer diagnosis. The results show that the system can distinguish malignant tissues from benign skin lesions with high diagnostic accuracy.
SCIENTIFIC REPORTS
(2022)
Article
Respiratory System
Lizzy Wijmans, Kirsten Kalverda, Daniel de Bruin, Paul Brinkman, Inge van den Berk, Joris J. T. H. Roelofs, Rene Jonkers, Peter I. I. Bonta, Jouke Annema
Summary: This study investigates the correlation between bronchoscopic CLE and OCT imaging with HRCT imaging in ILD. The results show that both CLE and OCT imaging provide more detailed anatomical information compared to HRCT. Real-time CLE during bronchoscopy can identify alveolar cells and lung fibrosis, while real-time OCT allows visualization of patchy fibrotic disease, honeycombing, and mucosal granulomas in the airways.
Article
Multidisciplinary Sciences
Stephan Allgeier, Andreas Bartschat, Sebastian Bohn, Rudolf F. Guthoff, Veit Hagenmeyer, Lukas Kornelius, Ralf Mikut, Klaus-Martin Reichert, Karsten Sperlich, Nadine Stache, Oliver Stachs, Bernd Koehler
Summary: This article describes a novel approach for real-time creation and visualization of a mosaic image of the SNP, allowing for evaluation of the acquired image data quality during the examination. Online mosaicking also offers the chance to identify overlap with previous images, which is particularly advantageous for follow-up examinations.
SCIENTIFIC REPORTS
(2022)
Article
Pharmacology & Pharmacy
Eloy Pena-Rodriguez, Teresa Garcia-Berrocoso, Ezequiel Vazquez Fernandez, Francisco J. Otero-Espinar, Joaquin Abian, Francisco Fenandez-Campos
Summary: Confocal Raman microscopy and MALDI-TOF mass spectrometry imaging are two promising techniques for ex vivo skin imaging and quantification. The biodistribution of dexamethasone (DEX) loaded lipomers in the skin was compared using both techniques, with benzalkonium chloride (BAK) as a tracer. Confocal Raman microscopy showed higher DEX measurements, while MALDI-TOF MSI was more suitable for tracing BAK. Confocal Raman microscopy provided higher spatial resolution and observed specific skin structures, while MALDI-TOF MSI had a faster sampling rate and analyzed larger tissue areas.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Engineering, Biomedical
Dazhao Zhou, Shang Sun, Ming Li, Xueping Liu, Shengli Mi
Summary: This research proposes a GAN-based super-resolution reconstruction model which significantly enhances the quality of eyelid tissue images by introducing a new attention mechanism and high-order degradation simulation. Compared to existing models, the proposed model demonstrates the strongest generalization ability and robustness, reducing reconstruction time and improving image quality for low and high-noise images.
BIOMEDICAL SIGNAL PROCESSING AND CONTROL
(2024)
Article
Geochemistry & Geophysics
Xuejun Huang, Jinshan Ding, Zhong Xu
Summary: This letter presents an unsupervised CNN-based framework for super-resolution ISAR imaging, which can directly produce high-resolution ISAR images in real time and is suitable for practical applications.
IEEE GEOSCIENCE AND REMOTE SENSING LETTERS
(2022)
Article
Urology & Nephrology
Jesus Z. Villarreal, J. Perez-Anker, S. Puig, G. Pellacani, M. Sole, J. Malvehy, Luis F. Quintana, A. Garcia-Herrera
Summary: This study evaluated the feasibility of using ex vivo confocal microscopy in non-neoplastic kidney biopsies, and found that confocal images correlated well with conventional histological sections without introducing artifacts to the samples. The ease and speed of image acquisition, along with the quality of digitally stained images obtained, suggest promise for clinical applications in nephrology and renal transplantation.
JOURNAL OF NEPHROLOGY
(2021)
Article
Chemistry, Multidisciplinary
Franco F. Faucher, Kevin J. Liu, Emily D. Cosco, John C. Widen, Jonathan Sorger, Matteo Guerra, Matthew Bogyo
Summary: Surgery is the preferred treatment for solid tumors, but inaccurate cancer border detection can result in incomplete removal or excessive excision of tissue. We describe a strategy to convert fluorescent probes into ratiometric contrast agents, improving tumor detection sensitivity. We also built a camera system for real-time imaging of ratiometric signals during surgery, showing the potential for clinical implementation to improve cancer resection.
ACS CENTRAL SCIENCE
(2023)
Article
Biology
Marius Kroger, Jorg Scheffel, Evgeny A. Shirshin, Johannes Schleusener, Martina C. Meinke, Jurgen Lademann, Marcus Maurer, Maxim E. Darvin
Summary: This study demonstrates a noninvasive method (TPE-FLIM) that can visualize macrophages in human dermis. The method shows high sensitivity and specificity, making it valuable for studying the role of macrophages in health and disease.
Article
Biotechnology & Applied Microbiology
Xinyang Li, Yixin Li, Yiliang Zhou, Jiamin Wu, Zhifeng Zhao, Jiaqi Fan, Fei Deng, Zhaofa Wu, Guihua Xiao, Jing He, Yuanlong Zhang, Guoxun Zhang, Xiaowan Hu, Xingye Chen, Yi Zhang, Hui Qiao, Hao Xie, Yulong Li, Haoqian Wang, Lu Fang, Qionghai Dai
Summary: DeepCAD-RT is a real-time denoising method for fluorescence time-lapse images, which uses self-supervised deep learning for noise suppression. Compared to traditional methods, DeepCAD-RT significantly reduces the number of network parameters, memory consumption, and processing time, and achieves high imaging signal-to-noise ratio with one-tenth of the photons. In various photon-limited experiments, DeepCAD-RT demonstrates its utility in morphological and functional interrogation of biological dynamics.
NATURE BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Aditi Sahu, Yuna Oh, Gary Peterson, Miguel Cordova, Cristian Navarrete-Dechent, Melissa Gill, Christi Alessi-Fox, Salvador Gonzalez, William Phillips, Steven Wilson, Reza Afzalneia, Raven Rose, Abu-Akeel Mohsere, Danielle Bello, Ashfaq Marghoob, Anthony Rossi, Jedd D. Wolchok, Taha Merghoub, Veronica Rotemberg, Chih-Shan Jason Chen, Milind Rajadhyaksha
Summary: The study explored the feasibility of performing 1-2 mm precision biopsies guided by high-resolution imaging techniques. Results showed that this biopsy method can ensure accurate diagnosis and molecular profiling in the presence of spatial heterogeneity, especially in tumors, providing a new strategy for next-generation histopathology and pathology.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Nina Ogrinc, Alexandre Kruszewski, Paul Chaillou, Philippe Saudemont, Chann Lagadec, Michel Salzet, Christian Duriez, Isabelle Fournier
Summary: The study developed a novel method for in vivo mass spectrometry imaging, using SpiderMass technology and a high-accuracy robotic arm. By moving the laser probe above the sample surface and equipping the robotic arm with a sensor, images can be acquired and molecular data can be plotted directly onto a 3D topographical image without the need for image fusion.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Adrian Phoulady, Nicholas May, Hongbin Choi, Yara Suleiman, Sina Shahbazmohamadi, Pouya Tavousi
Summary: This research proposes a method that uses femtosecond laser ablation as a delayering method in combination with optical and confocal microscopy for rapid 3D imaging. The use of confocal microscopy eliminates image distortion caused by non-flat layers and allows for maintaining layer height variations within a small range. The method has been applied to a printed circuit board (PCB) and validated and compared with X-ray CT imaging.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Weikai Xue, Jonas Ogien, Pavel Bulkin, Anne-Lise Coutrot, Arnaud Dubois
Summary: This study aimed to develop a more compact and lighter LC-OCT device that can acquire images faster without significant degradation of resolution. The device is based on a Mirau interferometer using a single objective and incorporates a galvanometer scanner for lateral scanning of the illumination line. The optimized device can acquire B-scans at an unprecedented rate of 17 fps and produce 3D images of human skin at cellular resolution.
JOURNAL OF BIOMEDICAL OPTICS
(2022)
Review
Chemistry, Multidisciplinary
Stefania Guida, Federica Arginelli, Francesca Farnetani, Silvana Ciardo, Laura Bertoni, Marco Manfredini, Nicola Zerbinati, Caterina Longo, Giovanni Pellacani
Summary: Confocal laser scanning microscopy (CLSM) is a valuable tool in clinical settings, providing a quasi-histologic view of tissues without the need for biopsy. It has shown to improve diagnostic accuracy of skin cancers and contribute to reducing unnecessary excisions, as well as improving margin recognition for tissue sparing in excisions. Further studies using CLSM in real-life settings could enhance our understanding of its applications.
APPLIED SCIENCES-BASEL
(2021)
Review
Biochemistry & Molecular Biology
Elena-Georgiana Dobre, Mihaela Surcel, Carolina Constantin, Mihaela Adriana Ilie, Ana Caruntu, Constantin Caruntu, Monica Neagu
Summary: Early diagnosis is crucial for effectively treating skin cancer. Emerging optical imaging techniques, such as RCM, OCT, MRI, NIR bioimaging, and PET, can provide non-invasive imaging data for early detection and monitoring of cutaneous tumors. These techniques can observe dynamic processes relevant to disease evolution, including blood flow, immune cell activation, and tumor energy metabolism. This review discusses the latest advances in imaging techniques for skin cancer detection and monitoring, highlighting the challenges and opportunities for their implementation in clinical practice and their potential to complement molecular and histological approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Carolina Constantin, Daniela Simina Stefan, Ana-Maria Manea-Saghin, Irina Meghea
Summary: Improving soil quality through the reuse and recycling of biopolymers from tannery waste has been proposed, with collagen hydrolysate and micronutrients as fertilizers. Polyacrylamide, starch, and dolomite were included to enhance their efficiency. These fertilizers were characterized and tested on poor soils, showing positive effects on plant growth and nodulation. The application dose for different crops was estimated. This study's novelty lies in the recovery and functionalization of waste materials into efficient fertilizers.
Article
Medicine, General & Internal
Ancuta Mihai, Diana Maria Chitimus, Ciprian Jurcut, Florin Cristian Blajut, Daniela Opris-Belinski, Constantin Caruntu, Ruxandra Ionescu, Ana Caruntu
Summary: This study aimed to evaluate the predictive potential of hematological and immunological parameters associated with peripheral neuropathy (PN) development in patients with Primary Sjogren syndrome (pSS). The results showed that neutrophils and neutrophil-to-lymphocyte ratio (NLR) were significantly higher in PN+ patients, while lymphocytes, monocytes, and monocyte-to-lymphocyte ratio (MLR) were significantly lower. Furthermore, immuno-inflammatory parameters such as gammaglobulins, complement fractions C3, C4, total proteins, and vitamin D were significantly lower in the PN+ group. Therefore, these biological parameters can be useful in predicting neurological involvement and monitoring disease progression in pSS patients.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Andreea Nicoleta Serbanica, Delia Codruta Popa, Constantin Caruntu, Sergiu Pasca, Cristian Scheau, Ionut Vlad Serbanica, Raluca Suciu, Valeria Tica, Elisa Busescu, Luminita Nicoleta Cima, Cerasela Jardan, Mihaela Dragomir, Daniel Coriu, Andrei Colita, Anca Colita
Summary: This retrospective study evaluated the expression of CD20 in 114 patients with B-cell precursor acute lymphoblastic leukemia (B-ALL), and found that CD20 expression was associated with disease evolution. The results suggest that CD20 expression level is an important factor in the assessment and treatment of pediatric patients with B-ALL.
JOURNAL OF CLINICAL MEDICINE
(2023)
Editorial Material
Health Care Sciences & Services
Mircea Tampa, Cristina Iulia Mitran, Madalina Irina Mitran, Simona Roxana Georgescu
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Review
Dermatology
Maria-Alexandra Visan, Constantin Caruntu, Raluca Simona Costache, Sorin George Tiplica, Daniel Octavian Costache
Summary: Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease that negatively impacts patients' quality of life. The understanding of its underlying mechanisms is still limited, but it is believed to involve genetic mutations, microbiome dysbiosis, cytokine upregulation, and environmental factors. There is a need for improved diagnostic methods and classification systems to personalize management and advance research in this debilitating condition.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Review
Nutrition & Dietetics
Carolina Constantin, Mihaela Surcel, Adriana Munteanu, Monica Neagu, Jean Christopher Chamcheu
Summary: Psoriasis, an autoimmune chronic inflammatory skin condition, is influenced by genetic factors, immune disturbances, and environmental factors, especially the Western lifestyle and overall diet. Obesity is identified as a significant risk factor for psoriasis, aggravating the disease, while weight reduction can improve clinical outcomes. The connection between obesity and psoriasis lies in the pro-inflammatory effects of white adipose tissue, which is an important endocrine organ. This paper emphasizes the importance of nutrition strategies in psoriasis management.
Review
Nutrition & Dietetics
Aristodemos-Theodoros Periferakis, Argyrios Periferakis, Konstantinos Periferakis, Ana Caruntu, Ioana Anca Badarau, Ilinca Savulescu-Fiedler, Cristian Scheau, Constantin Caruntu
Summary: Capsaicin, a phytochemical derived from Capsicum plants, shows important antimicrobial properties against a wide range of bacteria, fungi, and viruses. The prospects for future development using new formulations and drug delivery mechanisms are promising. The influence of capsaicin on somatostatin and substance P secretion and action offers interesting research possibilities.
Review
Health Care Sciences & Services
Dana Mihaela Mihele, Paul Andrei Nistor, Gabriela Bruma, Cristina Iulia Mitran, Madalina Irina Mitran, Carmen Elena Condrat, Mihaela Tovaru, Mircea Tampa, Simona Roxana Georgescu
Summary: Mast cells are not only IgE effectors of anaphylaxis, but also defenders against infections and toxins. They play a role in inflammation regulation and tissue repair but are also involved in allergies and various pathological conditions. Mast cell activation syndrome (MCAS) has been proposed to explain symptoms caused by mast cell activation, but there is still debate about the diagnostic criteria.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Review
Biology
Gabriela Loredana Popa, Cristina Iulia Mitran, Madalina Irina Mitran, Mircea Tampa, Clara Matei, Mircea Ioan Popa, Simona Roxana Georgescu
Summary: Acne vulgaris is a chronic inflammatory skin disease involving keratinization, sebum production, Cutibacterium acnes colonization, and inflammation. Recent studies have shown that there is an imbalance between oxidants and antioxidants in acne patients, indicating the involvement of oxidative stress in the pathogenesis of acne.
Review
Biochemistry & Molecular Biology
Dalia Bratu, Daniel Boda, Constantin Caruntu
Summary: Atopic dermatitis (AD) is a chronic inflammatory skin disease with a strong genetic component. Genetic studies have revealed defects in the cutaneous barrier as one of the main causes of AD. Recently, epigenetic studies have shown that environmental factors can influence gene expression, leading to changes in the chromatin structure. While there are currently no targeted therapies focusing on the epigenetic process of AD, miR-143 shows potential as a therapeutic target for restoring the cutaneous barrier defects.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
George-valentin Saftoiu, Carolina Constantin, Adrian-ionut Nicoara, George Pelin, Denisa Ficai, Anton Ficai
Summary: This article investigates the properties of glass fiber composite specimens made from a mixture of EPOLAM 2001 resin and hardener with different concentrations. The study also examines the influence of resin amount, hardener amount, drying time, and drying temperature on the final material properties.
UNIVERSITY POLITEHNICA OF BUCHAREST SCIENTIFIC BULLETIN SERIES B-CHEMISTRY AND MATERIALS SCIENCE
(2023)
Article
Health Care Sciences & Services
Dalia Bratu, Daniel Boda, Constantin Caruntu
Summary: Atopic dermatitis is a chronic inflammatory skin disease associated with multiple allergies. It is influenced by genetic, immune, and environmental factors. The incidence and prevalence of the disease have been increasing, particularly in developed countries. Non-invasive techniques such as reflectance confocal microscopy can be used to effectively manage and monitor patients with this heterogeneous disease. It allows for the visualization of dynamic changes in the skin during treatment and can help in the early detection of inflammatory processes. Further developments in reflectance confocal microscopy may contribute to improved diagnosis, staging, and treatment strategies for atopic dermatitis.
