Article
Virology
Jingjing Liang, Gordon Ruthel, Cari A. Sagum, Mark T. Bedford, Sachdev S. Sidhu, Marius Sudol, Chaitanya K. Jaladanki, Hao Fan, Bruce D. Freedman, Ronald N. Harty
Summary: The study identified the host WW-domain-containing protein WWOX as a novel interactor with VP40 and Z proteins, demonstrating that WWOX inhibits budding of VP40/Z VLPs and live virus in a PPxY/WW-domain-dependent manner. These findings expand the repertoire of host interactors regulating PPxY-mediated budding of RNA viruses and highlight competitive interplay and modular virus-host interactions impacting both the virus life cycle and the host cell.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Jingjing Liang, Gordon Ruthel, Bruce D. Freedman, Ronald N. Harty
Summary: Filoviruses (Ebola virus [EBOV] and Marburg virus [MARV]) are zoonotic, emerging pathogens that cause outbreaks of severe hemorrhagic fever in humans. A fundamental understanding of the virus-host interface is critical for understanding the biology of these viruses and for developing future strategies for therapeutic intervention.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jiarui Song, Claudia Castillo-Gonzalez, Zeyang Ma, Dorothy E. Shippen
Summary: The recent discovery of bona-fide telomerase RNA from plants has provided new insights into telomerase RNP with conserved and unique structural elements. Two highly conserved proteins in Arabidopsis, AtPOT1a and AtNAP57 (dyskerin), have been found to interact with plant telomerase. This study reveals that AtPOT1a stimulates telomerase activity by interacting with TERT, while dyskerin binds to AtTR through a plant-specific three-way junction, playing a crucial role in telomerase biogenesis and evolution.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Virology
Kosuke Oda, Yasuyuki Matoba, Masanori Sugiyama, Takemasa Sakaguchi
Summary: The study reveals the crucial role of the binding between the Respirovirus C protein and Alix in viral replication, with mutant C proteins of varying binding affinities showing impaired virus production. This interaction is essential for viral budding and provides insights for the development of new antiviral drugs against hPIV1.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Chih-Yen Lin, Aspiro Urbina, Wen-Hung Wang, Arunee Thitithanyanont, Sheng-Fan Wang
Summary: Viral assembly and budding are the final and crucial steps of the virus life cycle, regulated by virus-host interaction. Many viruses exploit their late assembly (L) domains to manipulate host machinery for replication.
Article
Biochemistry & Molecular Biology
Bo He, Yuan Ma, Fangfang Tian, Guang-Rong Zhao, Yi Wu, Ying-Jin Yuan
Summary: As an enabling technique of synthetic biology, the YLC-assembly method allows in vivo iterative assembly of large DNA through cell-cell transfer in the yeast life cycle. It exhibits a higher success rate and easier workflow compared to other methods, minimizing the need for in vitro operations. The YLC-assembly lowers the technical difficulty of assembling large DNA and has potential for large-scale genome engineering and synthetic genomics.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Microbiology
Victoria Ortega, J. Lizbeth Reyes Zamora, I. Abrrey Monreal, Daniel T. Hoffman, Shahrzad Ezzatpour, Gunner P. Johnston, Erik M. Contreras, Fernando J. Vilchez-Delgado, Hector C. Aguilar
Summary: This study uncovers new mechanisms by which the G stalk and its mobility modulate the triggering of F and also unexpectedly affect a very late step in membrane fusion, namely fusion pore expansion. These novel findings may extend to other paramyxoviruses, offering new potential targets for therapeutic interventions.
Article
Virology
Erik M. Contreras, Gunner P. Johnston, David W. Buchholz, Victoria Ortega, I. Abrrey Monreal, J. Lizbeth R. Zamora, Tracy Cheung, Hector C. Aguilar
Summary: Cholesterol is involved in various stages of the viral life cycle for enveloped viruses. Nipah virus (NiV) is a highly pathogenic enveloped virus in the Henipavirus genus within the Paramyxoviridae family, and cholesterol levels influence both cell-cell membrane fusion during syncytium formation and virus-cell membrane fusion during viral entry. The levels of cholesterol impact early and late steps during the membrane fusion cascade in NiV, expanding our understanding of viral entry and cell-cell fusion processes.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Amber Vogel, Alexandra Crawford, Afua Nyarko
Summary: This study successfully generated a polypeptide containing the complete set of three PPxY binding sites of AMOTL1 and investigated its binding mechanism with YAP WW domains using various biophysical techniques. The study revealed the formation of an ensemble of complexes with different stabilities between the AMOTL1 polypeptide and YAP WW domains, and the binding process was regulated by transient interactions.
Article
Cell Biology
Mariko Enshoji, Yoshiko Miyano, Nao Yoshida, Makoto Nagano, Minami Watanabe, Mayumi Kunihiro, Daria E. Siekhaus, Junko Y. Toshima, Jiro Toshima
Summary: This study demonstrates that anchoring the yeast Eps15-like protein Pan1p to the peroxisome can trigger most of the events occurring during the late phase of endocytosis. Pan1p recruits various proteins and initiates actin polymerization, ultimately completing the process of endocytosis.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemical Research Methods
Fengqi Ge, Chunxiang Peng, Xinyue Cui, Yuhao Xia, Guijun Zhang
Summary: AlphaFold2 achieves breakthrough in protein structure prediction with an end-to-end deep learning method, accurately predicting single-domain proteins. However, accuracy in predicting full-chain proteins is lower due to incorrect domain interactions. This study introduces DeepIDDP, an inter-domain distance prediction method, which incorporates attention mechanisms and new inter-domain features to enhance capturing domain interactions. Integration of DeepIDDP into the SADA domain assembly method improves inter-domain distance prediction accuracy by 11.3% and 21.6% compared to trRosettaX and trRosetta, and the domain assembly model outperforms SADA by 2.5%. Additionally, using DeepIDDP to reassemble human multi-domain protein models enhances average TM-score by 11.8%. The online server can be found at .
BRIEFINGS IN BIOINFORMATICS
(2023)
Article
Biochemistry & Molecular Biology
Soubhagyalaxmi Jema, Chu Chen, Lauren Humphrey, Shriya Karmarkar, Frank Ferrari, Ajit P. Joglekar
Summary: During mitosis, the recruitment of SAC signaling proteins to kinetochores plays a crucial role in preventing chromosome missegregation. This study reveals that the cellular abundance of the SAC signaling protein Bub1 limits its own recruitment and that of BubR1, thereby regulating the SAC signaling activity. The number of SAC proteins recruited by a signaling kinetochore is inversely correlated with the total number of signaling kinetochores in the cell.
Article
Virology
Zhaohuan Wang, Rui Li, Chenxi Liu, Wentao Qiao, Juan Tan
Summary: This study reveals that bovine foamy virus (BFV) recruits endosomal sorting complex required for transport (ESCRT) through the L domain motifs on the Gag protein for virus budding. The functional sites of the ESCRT components, ALIX and Tsg101, for BFV budding are identified. These findings contribute to a better understanding of the budding mechanism of foamy viruses.
Article
Biophysics
Rohit Joshi, Pavel Pohl, Dita Strachotova, Petr Herman, Tomas Obsil, Veronika Obsilova
Summary: Nedd4-2, a ubiquitin ligase, is involved in the endocytosis and degradation of target molecules through interactions with 14-3-3 proteins. The binding of 14-3-3 induces structural rearrangement of Nedd4-2, affecting the mobility and emission properties of certain domains. In contrast, the active site of the HECT domain becomes more mobile upon 14-3-3 binding.
BIOPHYSICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Paolo Rossi, Qiong Xing, Elisabetta Bini, Athina G. Portaliou, Mary C. Clay, Eric M. Warren, Nandish K. Khanra, Anastassios Economou, Charalampos G. Kalodimos
Summary: This article investigates the interaction of late-stage flagellar chaperones FliT and FlgN with the export controller protein FliJ in Salmonella enterica. The study finds that FliT and FlgN bind cooperatively to FliJ with high affinity at specific sites. It is discovered that the binding of chaperones disrupts the coiled-coil structure of FliJ and alters its interactions with the export gate. The researchers propose that FliJ aids in the release of substrates from chaperones and plays a role in chaperone recycling during late-stage flagellar assembly.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Virology
Greeshma Ray, Phuong Tieu Schmitt, Anthony P. Schmitt
JOURNAL OF VIROLOGY
(2016)
Article
Virology
Nicolas Cifuentes-Munoz, Weina Sun, Greeshma Ray, Phuong Tieu Schmitt, Stacy Webb, Kathleen Gibson, Rebecca Ellis Dutch, Anthony P. Schmitt
JOURNAL OF VIROLOGY
(2017)
Article
Microbiology
Jillian C. Carmichael, Hiroki Yokota, Rebecca C. Craven, Anthony Schmitt, John W. Wills
Article
Immunology
Karen Racicot, Anthony Schmitt, Troy Ott
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2012)
Review
Biochemistry & Molecular Biology
Megan S. Harrison, Takemasa Sakaguchi, Anthony P. Schmitt
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2010)
Article
Virology
Phuong Tieu Schmitt, Greeshma Ray, Anthony P. Schmitt
JOURNAL OF VIROLOGY
(2010)
Article
Virology
Zifei Pei, Megan S. Harrison, Anthony P. Schmitt
JOURNAL OF VIROLOGY
(2011)
Article
Virology
Megan S. Harrison, Phuong Tieu Schmitt, Zifei Pei, Anthony P. Schmitt
JOURNAL OF VIROLOGY
(2012)
Article
Virology
John B. Johnson, Anthony P. Schmitt, Griffith D. Parks
JOURNAL OF VIROLOGY
(2013)
Article
Virology
Weina Sun, Thomas S. McCrory, Wei Young Khaw, Stephanie Petzing, Terrell Myers, Anthony P. Schmitt
JOURNAL OF VIROLOGY
(2014)
Article
Biochemistry & Molecular Biology
T. Oka, A. P. Schmitt, M. Sudol
Review
Virology
Farah El Najjar, Anthony P. Schmitt, Rebecca Ellis Dutch
Article
Virology
Santosh Panthi, Phuong Tieu Schmitt, F. Jeffrey Lorenz, Brent A. Stanfield, Anthony P. Schmitt
Summary: A flexible platform for protein delivery to target cell interiors using paramyxovirus-like particles has been developed, enabling efficient cargo packaging and delivery to target cells. This approach holds promise for safe and efficient delivery of therapeutic proteins to cells and tissues, addressing current limitations in protein delivery for potential clinical applications.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lara Rheinemann, Diane Miller Downhour, Kate Bredbenner, Gaelle Mercenne, Kristen A. Davenport, Phuong Tieu Schmitt, Christina R. Necessary, John McCullough, Anthony P. Schmitt, Sanford M. Simon, Wesley I. Sundquist, Nels C. Elde
Summary: Duplicated and truncated copies of the ESCRT-III factor CHMP3 have been found in New World monkeys and mice, which can block virus budding. These new retroCHMP3 proteins have evolved to reduce interactions with other ESCRT-III factors, providing a new route for inhibiting the release of various viruses.