Journal
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
Volume 74, Issue 3-4, Pages 71-76Publisher
WALTER DE GRUYTER GMBH
DOI: 10.1515/znc-2018-0216
Keywords
6-hydroxyhexanoic acid; Baeyer-Villiger monooxygenase; Candida antartica Lipase B; cyclohexanone monooxygenase; enzyme cascade reactions; whole-cell biocatalysis
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Multi-enzyme cascade reactions capture the essence of nature's efficiency by increasing the productivity of a process. Here we describe one such three-enzyme cascade for the synthesis of 6-hydroxyhexanoic acid. Whole cells of Escherichia soli co-expressing an alcohol dehydrogenase and a Baeyer-Villiger monooxygenase (CHMO) for internal cofactor regeneration were used without the supply of external NADPH or NADP(+). The product inhibition caused by the epsilon-caprolactone formed by the CHMO was overcome by the use of lipase CAL-B for in situ conversion into 6-hydroxyhexanoic acid. A stirred tank reactor under fed-batch mode was chosen for efficient catalysis. By using this setup, a product titre of >20 g L-1 was achieved in a 500 mL scale with an isolated yield of 81% 6-hydroxyhexanoic acid.
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