Journal
ULTRASONICS SONOCHEMISTRY
Volume 51, Issue -, Pages 12-19Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ultsonch.2018.10.013
Keywords
Ultra sonication; Butterfly pea flower; Bioactive compounds; Conventional extraction; pBR322 plasmid DNA; Antioxidant activities
Categories
Funding
- National key research and development program [2018YFD0400403, 2016YFD0400802]
- National Natural Science Foundation of China (NSFC) [31571801]
- Beijing Municipal Science and Technology Project [Z171100002217019]
- Support Project of High-level Teachers in Beijing Municipal Universities in the Period of 13th Five-year Plan [CITTCD201704042]
- Beijing Technology and Business University
- Beijing Government Scholarship
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The present study was conducted to evaluate the influence of ultrasound on bioactive compounds and biological activities of blue butterfly pea flower (Clitoria ternatea L). For this purpose, optimized conditions (temperature 50 degrees C, time 150 min, solid to liquid ratio 1 g:15ml, 70% amplitude and 240 W, 20 kHz frequency, 3 s on and 3 s off) of ultrasound (US) and conventional extraction (AGE: Agitation, water bath for 150 min, 50 degrees C at 150 rmp) were used. The results showed significant (p < 0.05) effect of US and AGE on total phenolics (TPCs), flavonoids (TFCs) and antioxidant activities (DPPH, ABTS, FRAP, reducing activity, Cu2+ and H2O2) of butterfly pea flower extract (BPFE). The results showed an increased trend in yield, TPCs, TFCs and antioxidant activities of US treated BPFE with comparison to AGE. However, insignificant (p > 0.05) effect of US and AGE over TFlaCs and PACs were observed. Moreover, the results of Fourier-transform infrared spectroscopy (FTIR) showed little changes in spectrum and US does not affect the functional group of bioactive compounds structure. Additionally, extracts (500-2000 mu g/ml) protect pBR322 plasmid DNA damage induced by (1 mM H2O2 and 1 mM FeSO4), plasma oxidation (induced by 250 mu M CuCl2) and inhibit erythrocyte hemolysis (induced by 200 mM AAPH, 34.6 to 66.73%). Sonication can be applied successfully for the extraction of bioactive compounds from plant materials with high biological activities.
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