Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Ophthalmology
Satoko Nakano, Yasuhiro Tomaru, Toshiaki Kubota, Hiroshi Takase, Manabu Mochizuki, Norio Shimizu, Sunao Sugita
Summary: The Direct Strip PCR test offers rapid and accurate detection of infectious pathogens causing uveitis. It shows high concordance with qPCR results, even when used by beginners, indicating its reliability for clinical use. Additionally, it can be used for intraoperative diagnosis, improving the prognosis of infectious diseases.
Article
Parasitology
Gereon Schares, Majda Globokar Vrhovec, Mareen Tuschy, Maike Joeres, Andrea Baerwald, Bretislav Koudela, Jitender P. Dubey, Pavlo Maksimov, Franz J. Conraths
Summary: The 529-bp repeat of H. hammondi can be used to establish a quantitative real-time PCR assay, with excellent specificity and sensitivity. This assay is a valuable diagnostic tool for epidemiological and cell biological studies on H. hammondi and related parasites.
PARASITES & VECTORS
(2021)
Article
Optics
Damian Presti, Julian Bergier, Lucas Ripoll, Cristina Borio, Gustavo A. Torchia, Marcos Bilen
Summary: Since the global pandemic of SARS-CoV-2, the need for a low-cost and faster diagnostic tool for evaluating symptomatic patients has emerged. Point-of-care testing and point-of-need testing systems have been developed to provide accurate and rapid diagnostics. In this study, a bio-photonic device using an iso-thermal system was developed for detecting SARS-CoV-2. The device showed comparable sensitivity to the commercially used reference method and was built with simple and low-cost components, making it a high-efficiency and affordable instrument.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2023)
Article
Biochemical Research Methods
Renna L. Nouwairi, Larissa L. Cunha, Rachelle Turiello, Orion Scott, Jeff Hickey, Scott Thomson, Stuart Knowles, Jeff D. Chapman, James P. Landers
Summary: This article describes a field-forward prototype instrument capable of ultra-fast thermal cycling for real-time PCR amplification of DNA and RNA. The instrument can complete 40 cycles of real-time PCR in under 10 minutes, an 84% reduction in time compared to standard assays.
Article
Biochemical Research Methods
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hyejeong Jeong, Ki-Hun Jeong
Summary: A multi-channel plasmonic real-time reverse-transcription polymerase chain reaction system is used to demonstrate high-throughput and multi-target detection of the SARS-CoV-2 virus with four simultaneous reactions in a single assay within 15 min.
Article
Immunology
Wei-Hsuan Lin, Han-Cheng Chiu, Kuan-Fu Chen, Kuo-Chien Tsao, Yi-Yin Chen, Ting-Hsuan Li, Yhu-Chering Huang, Yu-Chia Hsieh
Summary: In adult patients with CAP, bacterial pathogens accounted for 50% and viruses for 36.3%. Molecular testing could detect more pathogens than traditional culture techniques in diagnosing pneumonia. Co-infections with multiple pathogens, especially K. pneumoniae and human rhinovirus, were common in this study.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Chemistry, Analytical
Padideh Mohammadyousef, Miltiadis Paliouras, Mark A. Trifiro, Andrew G. Kirk
Summary: In response to the need for rapid infectious pathogen detection, researchers have been working on adapting PCR for POC applications. This paper introduces a compact plasmonic PCR thermocycler for fast DNA amplification using photothermal heating with gold nanorods and a VCSEL. An ultrasensitive real-time amplicon detection strategy is also reported, based on monitoring UV absorption of the PCR sample cycle-by-cycle. This system allows for high detection sensitivity and does not require specific probes or fluorophores.
Article
Chemistry, Multidisciplinary
Shaw-Hwa Parng, Li-An Wu, Chien-Chih Kuo, Ping-Jung Wu, Yu-Yin Tsai, Chih-Hung Lee
Summary: This study proposes a method to generate droplets in a cylindrical tube for emulsion polymerase chain reaction. The validity of a non-planar droplet chip is verified by analyzing the light intensity of liquids in real-time during droplet processing. The study also compares two digital droplet PCR methods for measuring the copy numbers of EGFR.
Article
Agriculture, Multidisciplinary
Lin Yao, Hongmei Xin, Meng Qu, Yanhua Jiang, Yingying Guo, Fengling Li, Na Li, Zhijun Tan, Lianzhu Wang
Summary: The study developed a duplex real-time PCR method to specifically, sensitively, and accurately detect oilfish and escolar-derived components. The method showed high specificity without cross-reaction on other species, with a limit of detection for DNA of 0.0002 ng μL(-1) for R. pretiosus and 0.002 ng μL(-1) for L. flavobrunneum. Oilfish- and escolar-derived components were detected in 12 of the 70 commercial samples, consistent with DNA barcoding test results.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Food Science & Technology
Barbara Brezna, Jiri Smid, Tomas Kuchta
Summary: A novel real-time PCR method was developed for the DNA-based detection of pistachio nuts. The method exhibited high sensitivity and specificity, with a practical detection limit estimated to be between 0.002% and 0.01% in pastry samples. Further research is required to validate its compliance with the recent recommendations, but the method can currently be used for result confirmation or detection of intentional food adulteration.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Review
Medicine, General & Internal
Edinson Dante Meregildo-Rodriguez, Martha Genara Asmat-Rubio, Gustavo Adolfo Vasquez-Tirado
Summary: Tuberculosis is a global public health emergency. Improved diagnostic methods are urgently needed to address this epidemic. Droplet digital PCR (ddPCR) shows comparable diagnostic performance to qPCR for pulmonary tuberculosis, and better performance for extrapulmonary tuberculosis.
FRONTIERS IN MEDICINE
(2023)
Article
Microbiology
Daya Marasini, Melissa J. Whaley, Laurel T. Jenkins, Fang Hu, Wenxin Jiang, Nadav Topaz, Alex Chen, Susanna Schmink, Jennifer Dolan Thomas, Brian H. Harcourt, Henju Marjuki, Xin Wang
Summary: We have developed direct real-time PCR assays for the detection of Haemophilus influenzae (Hi) and its six serotypes directly from cerebrospinal fluid specimens. These assays are highly sensitive and specific, do not require DNA extraction, and can detect three serotypes in a single reaction, improving testing efficiency.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Dermatology
Hyeonji Seo, Ji Yeun Kim, Hyo-Ju Son, Jiwon Jung, Min Jae Kim, Yong Pil Chong, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Sung-Han Kim
Summary: Our study suggests that blood PCR can be a useful adjunct test for diagnosing patients with suspected invasive mould infection.
Article
Infectious Diseases
Joseph J. Modarelli, Julie Piccione, Pamela J. Ferro, Maria D. Esteve-Gasent
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2019)
Article
Entomology
Mackenzie Tietjen, Maria D. Esteve-Gassent, Raul F. Medina
JOURNAL OF MEDICAL ENTOMOLOGY
(2019)
Article
Infectious Diseases
Melina M. Ojeda-Chi, Roger Ivan Rodriguez-Vivas, Maria D. Esteve-Gasent, Adalberto A. Perez de Leon, Joseph J. Modarelli, Sandra L. Villegas-Perez
TRANSBOUNDARY AND EMERGING DISEASES
(2019)
Article
Infectious Diseases
Mariel Aguilar-Dominguez, Sokani Sanchez-Montes, Maria Dolores Esteve-Gassent, Carolina Barrientos-Salcedo, Adalberto Perez de Leon, Dora Romero-Salas
TICKS AND TICK-BORNE DISEASES
(2019)
Article
Veterinary Sciences
Joseph J. Modarelli, Mindy M. Borst, Julie Piccione, Maria D. Esteve-Gasent
VETERINARY CLINICAL PATHOLOGY
(2019)
Article
Infectious Diseases
Melina Maribel Ojeda-Chi, Roger Ivan Rodriguez-Vivas, Maria Dolores Esteve-Gasent, Adalberto Perez de Leon, Joseph J. Modarelli, Sandra Villegas-Perez
TICKS AND TICK-BORNE DISEASES
(2019)
Article
Multidisciplinary Sciences
Joseph J. Modarelli, Pamela J. Ferro, Adalberto A. Perez de Leon, Maria D. Esteve-Gasent
SCIENTIFIC REPORTS
(2019)
Article
Immunology
Wisam Salim Hassan, Paula R. Giaretta, Raquel Rech, Morgane Ollivault-Shiflett, Maria D. Esteve-Gasent
Article
Ecology
Serene Yu, Joseph Modarelli, John M. Tomecek, Justin T. French, Clayton Hilton, Maria D. Esteve-Gasent
INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE
(2020)
Article
Parasitology
Lais Sanseverino, Henrique Ortencio Filho, Maria Esteve-Gassent, Thais Martinez Rodrigues Jorge
REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL
(2019)
Article
Public, Environmental & Occupational Health
Eman M. Abouelhassan, Hamdy M. El-Gawady, Ahmad Anwar Abdel-Aal, Amal K. El-Gayar, Maria D. Esteve-Gassent
JOURNAL OF ARTHROPOD-BORNE DISEASES
(2019)