Journal
TALANTA
Volume 193, Issue -, Pages 184-191Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2018.09.103
Keywords
Electrochemiluminescent immunoassay; Environmental analysis; Diclofenac; Graphene oxide/PEDOT; Gold nanorods
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Funding
- National Natural Science Foundation of China [21175097, 31772053]
- Project of State Key Laboratory of Analytical Chemistry for Life Science [SKLACLS1716]
- Suzhou Industry, State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials [SYG201636]
- Priority Academic Program Development of Jiangsu Higher Education Institutions [YX10900212]
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Diclofenac (DCF) is among the pharmaceutical compounds of concern based on its nocuousness in environment. In this work, a novel electrochemiluminescent (ECL) immunosensor for detecting DCF was constructed using poly(etherimide)-poly(3,4-ethylene dioxythiophene): poly(styrene sulfonate) functionalized graphene oxide and CdSe@CdS quantum dots (QDs-PEI-GO/PEDOT) as bioreceptor for conjugating DCF antibody to magnify signal. It is worth noting that this is the first time GO/PEDOT has been applied to ECL sensor field. Compared with GO, GO/PEDOT exhibited a higher conductivity and more stable chemical property, indicating that the proposed immunosensor would possess stronger and more stable luminescence performance. In addition, the electrode was modified with gold nanorods (AuNRs) which increase the load capacity of DCF coating antigen through Au-N bond. Competitive immunoassay method was chosen for structuring immunosensor where polyclonal antibody (pAb) against DCF exhibited high-affinity recognition of DCF, which greatly improved the sensitively and selectivity of sensor. Consequently, the proposed immunosensor gratified in DCF detection with low detection limit (LODs) of 0.33 pg mL(-1) (S/N = 3), and displayed high stability and sensitivity, which initiated a new route for DCF determining.
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