Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 290, Issue -, Pages 676-683Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.03.114
Keywords
Fluorescent probe; Glutathione; Mitochondria; Lysosomes; Cyanine
Funding
- National Natural Science Foundation of China [21676113, 21402057, 21772054, 21472059]
- Distinguished Young Scholar of Hubei Province [2018CFA079]
- Youth Chen-Guang Project of Wuhan [2016070204010098]
- 111 Project [B17019]
- Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology (Shenzhen)
- State Key Laboratory of Materials-Oriented Chemical Engineering [KL17-10]
- Open Project Fund of Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules (Yanbian University) [NRFM201701]
- Ministry of Education
- foundation of Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, Jiangnan University [JDSJ2017-07]
- CCNU [CCNU18TS012]
- excellent doctoral dissertation cultivation grant of CCNU from the colleges' basic research and operation of MOE [2018YBZZ031, 2018YBZZ035]
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Fluorescent probes with novel organelle-labeling manner can contribute to verify the unraveling cellular functions of various organelles. In this work, we reported a glutathione (GSH) specific near-infrared fluorescent probe, which was composed of a near-infrared cyanine moiety, a morpholine-containing naphthalimide and a thiol-reactive site. The cationic fluorophore cyanine can be capable of targeting mitochondria while the morpholine group can serve as the lysosome-targeting role. The optical behavior in vitro showed that this probe showed high selectivity toward GSH over other thiol-containing amino acids in near-infrared channel. Similar selectivity was found in bioimaging of living cells. The fluorescence co-localization images confirmed that the probe had a good mitochondria-targeting and lysosome-targeting capability in living cells. Moreover, the probe was endowed with near-infrared emission which was favorable for marking the GSH in vivo. We anticipate that such dual targeting strategy will be helpful to develop multiple organelle-targeting fluorescent probes.
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