Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 282, Issue -, Pages 861-867Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.11.149
Keywords
miRNA detection; Graphene oxide; Colorimetric method; Paper sensor; Point of care testing
Funding
- National Research Foundation of Korea (NRF) - Ministry of Education [2017R1D1A1B03031616]
- Korea Institute of Toxicology [KK-1801-02]
- National Research Foundation of Korea [2017R1D1A1B03031616] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
MicroRNAs (miRNAs), short non-coding RNAs, have emerged as promising next-generation biomarkers of diverse diseases. In general, conventional quantitative real-time PCR and miRNA microarray methods have been utilized for the quantitative detection of miRNA. However, owing to their complicated procedures and expensive reagents/instruments, these methods cannot be widely applied by medical experts such as doctors and nurses, and are thus limited for practical clinical application. Here, we established a new graphene oxide (GO)-based NET strategy for facile miRNA detection with enhanced sensitivity, which is applicable for point-of-care testing (POCT). The proposed miRNA sensing strategy is fundamentally based on the peroxidase-mimicking DNAzyme (Dz) mediated colorimetric assay in response to target miRNA using rationally designed duplex molecular beacon (dMB). The application of GO as a net in this basic system (designated GONET) allows for gathering of the target-dependently activated Dz, which enhances the sensitivity and extends the applicability for POCT. The GONET system provides clear visualization of the target at the 10(-9) M scale with the naked eye without any complicated amplification steps.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available