Article
Plant Sciences
Jilin Chen, Shaoya Li, Yubing He, Jingying Li, Lanqin Xia
Summary: This article summarizes the latest developments in CRISPR/Cas-mediated homology-directed repair technology for plants, highlighting significant achievements in crop improvement. It describes various strategies to enhance repair efficiency, as well as the challenges and open questions in precision genome editing for both plant biological research and crop improvement.
Article
Plant Sciences
Guo-Qing Song, Grace Urban, John T. Ryner, Gan-Yuan Zhong
Summary: This study evaluated chimeric editing in gene editing, using a transgenic tobacco line and a CRISPR-Cas9 editing vector. It was found that chimeric editing primarily occurred during or after cell regeneration. Further transformation was conducted to enhance editing efficiency. The findings provide valuable information for understanding gene editing in plant cells using constitutively expressed CRISPR-Cas9 and gRNAs.
Article
Biochemistry & Molecular Biology
Ramona Gruetzner, Patrick Martin, Claudia Horn, Samuel Mortensen, Erin J. Cram, Carolyn W. T. Lee-Parsons, Johannes Stuttmann, Sylvestre Marillonnet
Summary: The recent discovery of the mode of action of the CRISPR/Cas9 system has provided biologists with a useful tool for generating site-specific mutations in genes of interest. In this study, optimizing the efficiency of the Cas9 nuclease in generating mutations in target genes by investigating its nucleotide and/or amino acid sequence features led to the finding that adding introns to the Cas9 coding sequence significantly improved editing efficiency. The intronized Cas9 gene was shown to be effective in generating mutant phenotypes in various plants, highlighting the importance of sequence modifications for enhancing CRISPR/Cas9 genome editing.
PLANT COMMUNICATIONS
(2021)
Review
Plant Sciences
Koushik Halder, Abira Chaudhuri, Malik Z. Abdin, Manoj Majee, Asis Datta
Summary: RNA interference (RNAi) and CRISPR/Cas9 are important biotechnological tools in the field of agriculture. RNAi can improve disease resistance in plants, while CRISPR/Cas9 can be used to develop virus-resistant plants. In addition, the use of RNAi-induced dsRNA has great potential in various applications.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Cell Biology
Heba M. Mansour, Aiman S. El-Khatib
Summary: This article reviews the pathogenesis of Parkinson's disease (PD) and its association with CRISPR/Cas9 gene editing technology. It introduces the kinases encoding genes related to PD and targeted control by CRISPR/Cas9 technology. It also discusses the off-target effects of the CRISPR/Cas9 system and how to address them, as well as the challenges and future directions in kinase research for PD.
AGEING RESEARCH REVIEWS
(2023)
Review
Biochemistry & Molecular Biology
Chao Zhang, Shanhe Liu, Xuan Li, Ruixuan Zhang, Jun Li
Summary: CRISPR/Cas-based genome editing technologies have revolutionized plant science by allowing precise manipulation of plant genomes. Virus-induced genome editing (VIGE) has emerged as a powerful method for delivering CRISPR/Cas reagents into plant cells, with high editing efficiency and simplified process. This article provides an overview of CRISPR/Cas-based genome editing and focuses on the VIGE systems, types of viruses used for CRISPR/Cas9 cassette delivery, recent applications, and challenges and potential of VIGE in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Xing Yin, Romain Harmancey, David D. McPherson, Hyunggun Kim, Shao-Ling Huang
Summary: The efficient delivery of CRISPR/Cas remains a challenge in gene editing, but liposomes provide a promising approach to enhance the delivery of CRISPR/Cas and guide RNA. Liposomes facilitate endosomal escape and offer spatial and temporal control of cargo release, making them an effective tool for precise and efficient genetic modifications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Mujeeb Ur Rahman, Muhammad Bilal, Junaid Ali Shah, Ajeet Kaushik, Pierre-Louis Teissedre, Malgorzata Kujawska
Summary: Parkinson's disease and other neurodegenerative diseases have a significant impact on individuals and society. Recent research has identified neuroinflammation, oxidative stress, dysregulated protein degradation, and mitochondrial dysfunction as primary causes of Parkinson's disease. Gene-editing technologies hold promise for new treatment options.
Article
Biochemical Research Methods
Guoliang Yuan, Haiwei Lu, Kuntal De, Md Mahmudul Hassan, Yang Liu, Yi Li, Wellington Muchero, Paul E. Abraham, Gerald A. Tuskan, Xiaohan Yang
Summary: This study developed a strategy based on split inteins to achieve targeted genome editing in plant cells by splitting the required CRISPR/Cas9 components across a dual-vector system. The effectiveness of the split system was validated using a plant-based biosensor, and several split sites were identified for future biodesign strategies.
ACS SYNTHETIC BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Si-Wei Wang, Chao Gao, Yi-Min Zheng, Li Yi, Jia-Cheng Lu, Xiao-Yong Huang, Jia-Bin Cai, Peng-Fei Zhang, Yue-Hong Cui, Ai-Wu Ke
Summary: The CRISPR/Cas9 gene editing technology has made significant progress in oncological research, providing a powerful tool for exploring the mechanism of cancer, establishing tumor models, and studying drug targets.
Review
Materials Science, Multidisciplinary
Shao Wei Hu, Tao Ding, Honghai Tang, Huiping Guo, Wenguo Cui, Yilai Shu
Summary: With the advancement of genome editing techniques, gene therapy is increasingly being used for the treatment of various human diseases. Nanomaterials have shown great potential in improving the efficiency and safety of gene editing in gene therapy. This review introduces three gene editing tools, with a particular focus on the development and optimization of the CRISPR system. It also discusses the recent application of nanobiomaterials in gene therapy, along with the prospects and remaining challenges. Lastly, future directions for gene editing tools, nanobiomaterial vectors, and gene therapy are presented.
Review
Pharmacology & Pharmacy
Daniel Allen, Nechama Kalter, Michael Rosenberg, Ayal Hendel
Summary: Genome engineering through targeted nucleases, especially CRISPR-Cas9, has revolutionized gene therapy research and has the potential to treat blood and immune system diseases. CRISPR-Cas9 homology-directed repair (HDR) represents a promising method for site-specific gene insertion or correction. Other methods, like viral gene addition, gene knockout through non-homologous end joining (NHEJ), and base or prime editing, also show promise but have significant limitations for treating genetic immune or blood disorders. This review aims to highlight the transformative benefits of HDR-mediated gene therapy and propose solutions for current challenges, in order to advance HDR-based gene therapy in CD34(+) hematopoietic stem progenitor cells (HSPCs).
Article
Agriculture, Dairy & Animal Science
Kamlesh Kumari Bajwa, Meeti Punetha, Dharmendra Kumar, Prem Singh Yadav, Chares R. Long, Naresh L. Selokar
Summary: In this study, the optimization of electroporation conditions allowed for successful editing of the ITGB2 gene in adult buffalo fibroblasts, achieving high editing efficiency. These electroporation conditions can be applied to various biotechnological research applications for buffalo genes.
ANIMAL BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Qiaoyun Qi, Bichun Hu, Weiyu Jiang, Yixiong Wang, Jinjiao Yan, Fengwang Ma, Qingmei Guan, Jidi Xu
Summary: Plant epistatic regulation involves DNA methylation, non-coding RNA regulation, and histone modification to regulate gene expression and plant growth without altering the genome sequence. This review summarizes the recent progress of CRISPR/Cas9 in epigenome editing and discusses its potential applications in crop breeding and gene expression. The CRISPR/Cas9 system is known for its high editing efficiency and rapid translation of results, making it a valuable tool for plant epigenetic modification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Ganna Reint, Zhuokun Li, Kornel Labun, Salla Keskitalo, Inkeri Soppa, Katariina Mamia, Eero Tolo, Monika Szymanska, Leonardo A. Meza-Zepeda, Susanne Lorenz, Artur Cieslar-Pobuda, Xian Hu, Diana L. Bordin, Judith Staerk, Eivind Valen, Bernhard Schmierer, Markku Varjosalo, Jussi Taipale, Emma Haapaniemi
Summary: Precision CRISPR gene editing can be improved by fusing DNA repair proteins with Cas9, with different fusion proteins showing varying effects based on cell type and genomic site. Optimization is necessary to account for the diverse factors contributing to locus-specific genome editing outcomes.
Article
Plant Sciences
Xiaoyan Wang, Jiong Gao, Zheng Zhu, Xianxin Dong, Xiaolei Wang, Guodong Ren, Xin Zhou, Benke Kuai
Article
Genetics & Heredity
Xiaoyan Wang, Shuxin Zhang, Yongchao Dou, Chi Zhang, Xuemei Chen, Bin Yu, Guodong Ren
Article
Multidisciplinary Sciences
Xiaoyan Wang, Yuan Wang, Yongchao Dou, Lu Chen, Junli Wang, Ning Jiang, Chunce Guo, Qingqing Yao, Chizao Wang, Lin Liu, Bin Yu, Binglian Zheng, Julia A. Chekanova, Jinbiao Ma, Guodong Ren
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Plant Sciences
Xiaoyan Wang, Xintong Xu, Xiaowei Mo, Luyao Zhong, Jiancong Zhang, Beixin Mo, Benke Kuai
Letter
Biology
Wenwen Kong, Xianxin Dong, Yongbing Ren, Yuan Wang, Xintong Xu, Beixin Mo, Yu Yu, Xiaoyan Wang
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Plant Sciences
Kangwen Lao, Yinlin Xiao, Qing Huang, Beixin Mo, Xianxin Dong, Xiaoyan Wang
Summary: In this study, ectopic expression of the FT gene along with the Cas9 cassette promoted fast reproduction of T1 transgenic plants, and the late-flowering phenotype served as a convenient marker for screening transgene-free plants in the T2 segregation population.
PLANT CELL REPORTS
(2023)
Article
Multidisciplinary Sciences
Xiaoyan Wang, Wenwen Kong, Yuan Wang, Jinhui Wang, Luyao Zhong, Kangwen Lao, Xianxin Dong, Dingyu Zhang, He Huang, Beixin Mo, Yu Yu, Guodong Ren
Summary: In plants, HESO1 and URT1 cooperate with the cytoplasmic RNA degradation pathway component SKI2 to regulate photosynthesis by monitoring the RNA of the Calvin cycle gene TKL1. Dysfunction of HESO1, URT1, and SKI2 leads to leaf etiolation and reduced photosynthetic efficiency, accompanied by the production of illegitimate siRNAs that affect TKL expression and activity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Letter
Plant Sciences
Yongbing Ren, Xuan Ma, Bo Song, Xiaolan Yang, You Chen, Yu Yu, Xuemei Chen, Beixin Mo, Xiaoyan Wang
Summary: HEN1 SUPPRESSOR1 is crucial for stabilizing P4RNA through 3'-end uridylation.
Article
Plant Sciences
Jianbo Song, Xiaoyan Wang, Bo Song, Lei Gao, Xiaowei Mo, Luming Yue, Haiqi Yang, Jiayun Lu, Guodong Ren, Beixin Mo, Xuemei Chen
