Journal
ANALYTICAL SCIENCES
Volume 32, Issue 3, Pages 371-374Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.32.371
Keywords
Nucleic acid; extraction; polymerase chain reaction; cartridge; magnetic beads
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Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT Japan)
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We have developed a rapid, automated nucleic acid purification device in a single cartridge containing silica-coated magnetic beads. We succeeded in extracting the matrix protein gene of influenza A virus from pharyngeal swab samples within 3 min. The device will be widely applicable to detect a specific gene from the various samples for clinical diagnosis and genetic research.
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