Article
Biochemical Research Methods
Fayaj A. Mulani, Sharvani S. Nandikol, Jagadeesh S. Kajjihundi, Niranjana Pathappa, Sharanappa Puttappa, Hirekodathakallu Thulasiram
Summary: Meliaceae plants are widely used in agriculture, folklore, and traditional medicine, and contain diverse limonoids with various bioactivities. Accurate detection of these compounds from plant tissue extracts or biological samples is challenging. In this study, a new method was developed for the identification, quantification, and characterization of limonoids in different plant tissue extracts using minimal plant material.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Plant Sciences
Maryam Mehmood, Marina Perez-Llorca, Andrea Casadesus, Sumaira Farrakh, Sergi Munne-Bosch
Summary: Phytohormones such as cytokinins and auxin play major roles in modulating leaf growth in sesame plants, while a putative antagonistic response between jasmonic acid and salicylic acid was observed during leaf development. Additionally, cytokinins were found to modulate growth arrest during water deficit stress, suggesting a metabolic limitation in cytokinin biosynthesis under drought conditions.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Food Science & Technology
Rukshan Mehta, Sweekruthi A. Shetty, Melissa F. Young, P. Barry Ryan, Kannan Rangiah
Summary: A validated UHPLC/MS-SRM method was developed for quantification of aflatoxins and ochratoxins in animal milk. Results showed that 90% of milk samples exceeded the EU limits, with 40% exceeding the Food Safety Standards Authority of India limits.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2021)
Article
Chemistry, Analytical
Zhanhua Hu, Jiali Li, Aixiang Xiao, Juntao Zheng, Shaoxing Guan, Jianxiong Guo, Min Huang
Summary: A rapid and sensitive method was established for simultaneous quantification of Escitalopram and its metabolites in human plasma. The method was validated and found to be in compliance with applicable regulations. The plasma concentrations of Escitalopram and its metabolites varied among depressed patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Rune Slimestad, Amritha Johny, Mette Goul Thomsen, Christian Rene Karlsen, Jan Thomas Rosnes
Summary: The present study evaluated the antimicrobial and antioxidant properties of extracts from nine herb species. The results showed that these herb extracts have potential as natural antimicrobials and antioxidants.
Article
Agriculture, Multidisciplinary
Yiming Zhang, Lanlan Zhan, Quan Wen, Yulin Feng, Yun Luo, Ting Tan
Summary: This study discovered that taxifolin is able to effectively trap methylglyoxal (MGO), an important precursor of advanced glycation end products (AGEs), by forming MGO adducts in vitro and in vivo. Quantification studies showed that taxifolin and its metabolites can dose-dependently trap MGO. Similar results were observed in diabetic mice.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Analytical
Zhenzhen Xie, James D. Morris, Stephanie J. Mattingly, Saurin R. Sutaria, Jiapeng Huang, Michael H. Nantz, Xiao-An Fu
Summary: Analysis of volatile organic compounds (VOCs) in exhaled breath has shown great potential for disease detection. However, analyzing metabolic VOCs in breath is still a challenge. This study presents a method using ultrahigh-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-MS) to analyze a broad range of carbonyl metabolites in breath. The combination of a silicon microreactor for capturing carbonyl compounds and UHPLC-MS analysis may provide a quantitative method for identifying disease markers in exhaled breath.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Bowen Huang, Adabelle Ong, Wenli Wang, Yu He, Yongjun Xiao
Summary: The detection and understanding of emerging contaminants (ECs) pose new challenges to water pollution control. Triclosan (TCS), an emerging contaminant widely found in the environment, is a commonly used antibacterial agent. Microdialysis (MD), a sampling technique, combined with UHPLC-QTOF/MS analysis, was used to identify endogenous metabolites as biomarkers of TCS exposure in living crucian carp. The quantification of these biomarkers using UHPLC-MS/MS provided continuous monitoring of the effect of TCS on endogenous metabolites, contributing to a better understanding of its toxicological effect.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Delia Castilla-Fernandez, David Moreno-Gonzalez, Ma Carmen Murillo-Cruz, Juan F. Garcia-Reyes, Antonio Molina-Diaz
Summary: The study evaluated three different clean-up strategies combined with QuEChERS extraction for determining fipronil and its metabolites in chicken eggs, with EMR-Lipid showing the best results.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Lina Wu, Liying Zhou, Di Wen, Xin Wang, Ping Xiang, Yan Shi
Summary: An efficient and rapid UHPLC-MS/MS method was developed for the detection of 87 synthetic cannabinoids and their metabolites in urine. The method showed high accuracy, good stability, and is applicable to a variety of analytes in forensic cases.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemical Research Methods
Bangjian Dong, Chongsheng Peng, Ping Ma, Xiaobo Li
Summary: This study comprehensively analyzed the non-polysaccharide components of Sijunzi decoction using advanced techniques, identifying 449 components, including potentially novel substances. By establishing an MS-network of SJZD and quantifying 19 representative components using UHPLC-QTRAP®-MS/MS, the pharmacological effects and action mechanism of SJZD were further understood.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Wei Zuo, Zhi Wu, Huan Xiong, Huyi Zhou, Chengqiu Wang, Jing Li
Summary: A fast and sensitive method for simultaneously detecting the nematicide fluensulfone and its two major metabolites in different types of agricultural soils was established using ultra-high performance liquid chromatography-tandem mass spectrometry. The method demonstrated high recoveries and precision, with an overall average recovery range of 73.1%-113.9% and relative standard deviations of less than 12.7%. The method was successfully applied to examine the degradation of the nematicide and the formation of its metabolites in three different types of soil, indicating its efficacy in studying the environmental behavior of the nematicide in agricultural soil.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Environmental Sciences
Antonio Cascajosa-Lira, Concepcion Medrano-Padial, Silvia Pichardo, Jose Manuel de la Torre, Alberto Banos, Angeles Jos, Ana M. Camean
Summary: This study focused on analyzing the toxicokinetic profile of propyl-propane-thiosulfonate (PTSO) in the agricultural food sector. In silico and in vitro techniques were used to perform a pilot-study, and ecotoxicity studies were conducted to evaluate the environmental impact of the compound. The results showed the formation of detoxified metabolites and highlighted the need for further in vivo assays to confirm the findings.
ENVIRONMENTAL RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Federica Pigliasco, Sara Malaca, Alfredo Fabrizio Lo Faro, Anastasio Tini, Giuliana Cangemi, Alessia Cafaro, Sebastiano Barco, Antonella Riva, Angelica Pisati, Elisabetta Amadori, Pasquale Striano, Adriano Tagliabracci, Marilyn Ann Huestis, Francesco Paolo Busardo
Summary: Cannabidiol (CBD) has anti-inflammatory, anxiolytic, antiseizure, and neuroprotective properties without addictive or psychotropic side effects. A VAMS method combined with ultra-high-performance liquid chromatography coupled with mass spectrometry was used to quantify CBD, THC, and their respective metabolites. The highest concentration was found in CBD-7-oic acid (7-COOH-CBD), followed by 7-hydroxy-cannabidiol (7-OH-CBD) and CBD. This analysis method is useful for studying epilepsy patients treated with CBD.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Food Science & Technology
Zhi Wu, Yuan Ma, Huan Xiong, Wenjin An, Yaohai Zhang, Qiyang Zhao, Jing Li
Summary: The present study applies a UHPLC-MS/MS method to simultaneously analyze spiropidion and its major metabolites in sweet orange fruit and by-products during the industrial juicing process. The method utilizes r-DSPE with MWCNTs for extraction and purification, and has been validated for its effectiveness. The study also investigates the effect of juicing processing on spiropidion residue and determines various processing factors. The results highlight the importance of the method in routine control analysis and further research on spiropidion residues in sweet orange products.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Gang Qu, Yuxin Zhao, Qiaoli Zhang, Jina Wu, Xiaosen Li, Yang Yang, Shilei Liu
Summary: In this study, magnetic mesoporous materials combined with real-time in situ mass spectrometry were used for the high-throughput detection of hydrolyzed products of organophosphorus nerve agents. The method showed good linearity, low limits of detection and quantification, and high extraction recoveries. The magnetic preparation method used was quick, cost-effective, rugged, and safe. The results demonstrated the potential of this method for rapid and efficient determination of the target analytes in environmental samples.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Anna Hildebrand, Mariam Merchant, Danny O'Hare
Summary: Substandard and falsified artemisinin derivatives in antimalarials have caused significant deaths and economic losses. This study evaluates the feasibility of voltammetric methods for identifying and quantifying artemether. The findings suggest that electrochemical analysis shows promise as a method for artemether identification and quantification.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Marx Osorio Araujo Pereira, Alvaro Ferreira Junior, Edson Silvio Batista Rodrigues, Helena Mulser, Giovanna Nascimento de Mello e Silva, Wallans Torres Pio dos Santos, Eric de Souza Gil
Summary: Brazilian spotted fever (BSF) is a serious and rapidly evolving disease. A new impedimetric immunosensor was developed for rapid diagnosis by measuring specific antibodies in plasma. The sensor demonstrated selectivity and accuracy, and has potential for important applications in diagnostic testing.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Kathrin Schilling, Ronald A. Glabonjat, Olgica Balac, Marta Galvez-Fernandez, Arce Domingo-Relloso, Vesna Slavkovich, Jeff Goldsmith, Miranda R. Jones, Tiffany R. Sanchez, Ana Navas-Acien
Summary: Analysis of trace elements in urine is an important tool for assessing exposures, diagnosing nutritional status, and guiding public health and healthcare intervention. This study provides a sensitive method for analyzing 18 elements in urine samples, using only 100 μL. The results show good accuracy and sensitivity of the method.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Mengya Li, Shijie Liu, Shiliang Guo, Dong Liang, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Jong-Hoon Lee, Gaiming Zhao, Yangyang Ma, Yanxia Liu
Summary: In this study, a magnetic flow device was developed to purify spores in a culture medium system. The device used magnetic nanoparticles to absorb vegetative cells, separating them from the spores. The achieved purity of the collected spores was over 95%. The study also demonstrated a rapid quantitative detection method using Raman spectroscopy.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Wanqiong Liu, Zixuan Wu, Jianwei Peng, Zebin Xu, Yong Liang
Summary: Metal-organic frameworks (MOFs) are effective carriers for molecular imprinting, but their poor dispersibility in aqueous solution is a significant drawback. In this study, we have applied amphiphilic block copolymers and molecularly imprinted technology on MOFs to improve the hydrophilicity of molecularly imprinted fluorescent materials.
ANALYTICAL METHODS
(2024)
