Article
Acoustics
Tong Zhang, Jie Wang, Jiaqi Feng, Yaqiong Liu, Ran Suo, Jingyu Jin, Wenxiu Wang
Summary: This study investigated the effects of different ultrasonic pretreatments on the gel properties of shrimp surimi. The results showed that proper ultrasound treatment could improve the gel properties by expanding the protein and exposing more functional groups. The study provides a new technical means to improve the gel properties of shrimp surimi.
ULTRASONICS SONOCHEMISTRY
(2022)
Article
Fisheries
Pablo Intriago, Andres Medina, Jorge Espinoza, Xavier Enriquez, Kelly Arteaga, Luis Fernando Aranguren, Andrew P. P. Shinn, Xavier Romero
Summary: High and sudden mortality of Penaeus vannamei post-larvae stage 1+ in Latin American hatcheries is caused by a Vibrio species carrying VpPirAB genes. This study investigated the matter by analyzing samples from diseased and healthy shrimp in commercial hatcheries. The disease progression involves cellular sloughing and necrosis, with an early phase characterized by hemocyte infiltration and a later phase characterized by massive sloughing and loss of the peritrophic membrane. Molecular and histological studies are needed to confirm the disease's cause, tentatively named post-larvae acute hepatopancreatic necrosis disease (PL-AHPND) to distinguish it from other pathologies.
AQUACULTURE INTERNATIONAL
(2023)
Article
Fisheries
Nuriyeh Mirzaei, Seyed Mohammad Mousavi, Vahid Yavari, Morteza Souri, Hossein Pasha-Zanoosi, Annahita Rezaie
Summary: The present study evaluated the quality of Litopenaeus vannamei postlarvae produced in different commercial hatcheries in Chouebdeh Abadan, Iran. The results showed that hatchery no. 3 had the best management practices, leading to better morphometric indices, stress test performance, behavioral index, and carcass biochemical composition in the postlarvae compared to other hatcheries. Proper management in hatchery no. 3 was identified as the key factor for higher quality in postlarvae production.
Article
Fisheries
Feng Yang, Limei Xu, Wanzhen Huang, Fang Li
Summary: A new lethal disease called glass post-larval disease has emerged in shrimp hatcheries in China since late 2019. This disease affects 6 - 12-day-old post-larvae of Penaeus vannamei and causes over 90% mortality within 24 - 48 hours. The disease is caused by highly lethal Vibrio parahaemolyticus strains and is a serious threat to shrimp aquaculture.
Article
Fisheries
Guowei Liao, Qiuping Wu, Binhua Mo, Jiang Zhou, Junyi Li, Jixing Zou, Lanfen Fan
Summary: This study investigated the impact of Vibrio alginolyticus on the intestinal histomorphology and microbiome composition in Litopenaeus vannamei. The results showed that Vibrio alginolyticus infection caused exfoliation of intestinal mucosal epithelial cells and altered the tissue morphology of the intestine. It also affected the abundance and diversity of intestinal microbiome.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Jiamin Feng, Yongxiong Huang, Meiling Huang, Junliang Luo, Liwen Que, Shiping Yang, Jichang Jian
Summary: In this study, a novel C-type lectin (CTL) called perlucin-like protein (PLP) was identified in Litopenaeus vannamei. PLP could be activated in response to Vibrio harveyi infection and could bind and agglutinate various bacteria. PLP was involved in stabilizing the expression of immune-related and apoptosis genes and reducing bacterial load in the hepatopancreas.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Environmental Sciences
Nanxiong Chen, Jiaojiao Jin, Yibin Chen, Yicheng Hu, Yuchun Shen, Sedong Li
Summary: This study compared the transcriptome of Litopenaeus vannamei Zoea larvae and adults infected by Vibrio parahaemolyticus using high-throughput Illumina sequencing. The results showed that pathways involved in the innate immune response were more enriched in the Zoea stage compared to the adult stage, suggesting the existence of innate immunity against Vibrio vulnificus during the Zoea larvae stage. These findings provide useful information for the study of innate immunity in shrimp larvae.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Fisheries
Yunhao Yuan, Haoxiang Guan, Yongxiong Huang, Junliang Luo, Jichang Jian, Shuanghu Cai, Shiping Yang
Summary: NF-E2-related factor-like-2 (Nrf2), a transcription factor belonging to the Cap'n'Collar transcription factor family, plays a role in regulating inflammation, autophagy, metabolism, proteostasis, and cancer prevention. The effects of Nrf2 on the immune response in Vibrio spp infection were investigated. The results showed that Nrf2 RNAi decreased antioxidant-related genes expression and up-regulated inflammation-related genes and apoptosis gene. Histopathological analysis revealed tissue damage in Nrf2 RNAi group, while SFN group exhibited lessened tissue damage and fewer apoptosis signals.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Chemistry, Medicinal
Xinjia Lv, Shihao Li, Yang Yu, Xiaojun Zhang, Fuhua Li
Summary: This study reveals that a crustin called LvCrustin I-2 can resist bacterial infection by regulating the balance of intestinal microbiota and has an impact on the intestinal epithelial barrier and immune function in shrimp.
Article
Fisheries
Xin Zhang, Jialong Shi, Yulong Sun, Yilei Wang, Ziping Zhang
Summary: This study discovered the immune regulation mechanism of shrimp in response to Vibrio parahaemolyticus infection after eyestalk ablation. RNASeq and KEGG analysis revealed that eyestalk ablation might activate the neuroendocrine-immune system. The expression of genes involved in the prophenoloxidase activating system and TLR signaling pathway were affected by eyestalk ablation. These findings shed light on the immune response mechanism of shrimp and provide a framework for building crustacean immunity theory.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biochemical Research Methods
Kristina Kappel, Joanna Fafinska, Markus Fischer, Jan Fritsche
Summary: This study describes the development of a rapid and easy-to-use DNA microarray assay for authenticating giant tiger prawns and whiteleg shrimp. By analyzing the signal patterns of 47 crustacean specimens, the specificity of the DNA microarray for the target species was demonstrated.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Acoustics
Tong Zhang, Jie Wang, Jiaqi Feng, Yaqiong Liu, Ran Suo, Qianyun Ma, Jianfeng Sun
Summary: This study evaluated the effects of ultrasound, microwave, and ultrasonic-microwave combination treatment on shrimp surimi gel properties. The results showed that ultrasound and microwave treatments improved the compactness of the gel structure and inhibited the degradation of myofibrillar protein.
ULTRASONICS SONOCHEMISTRY
(2022)
Article
Microbiology
Zhongyan Wang, Jude Juventus Aweya, Defu Yao, Zhihong Zheng, Chuanqi Wang, Yongzhen Zhao, Shengkang Li, Yueling Zhang
Summary: This study found significant changes in the metabolic profiles of shrimp at different stages of infection, with diseased shrimp showing high levels of taurine and moribund shrimp showing high levels of hypoxanthine. There was also a strong negative correlation between taurine levels and the relative abundance of Vibrio parahaemolyticus and Vibrio harveyi. Exogenous supplementation of taurine enhanced the survival of shrimp against V. parahaemolyticus infection.
Article
Multidisciplinary Sciences
Lian Gan, Jianwei Zheng, Wei-Hua Xu, Jianhao Lin, Jingshu Liu, Yu Zhang, Zizhan Wu, Zhaolin Lv, Youming Jia, Qingqi Guo, Shijun Chen, Chuanhe Liu, Tom Defoirdt, Qiwei Qin, Yiying Liu
Summary: This study found that bacterial contamination can cause muscle spoilage in crustaceans by changing the microbial composition, and suggested that crustaceans can be used as sensitive bio-detectors to indicate the extent of microbial contamination.
SCIENTIFIC REPORTS
(2022)
Article
Fisheries
Chen Wang, Peng-Fei Li, Da-Gang Hu, Hui Wang
Summary: In this study, the impact of Clostridium butyricum feeding on intestinal microorganisms and protection against Vibrio alginolyticus infection in Penaeus vannamei was evaluated. The results showed that Clostridium butyricum significantly altered the abundance of intestinal microbial populations and reduced Vibrio spp. Furthermore, the Clostridium butyricum-fed group exhibited improved survival rate, reduced pathological changes, and enhanced immune response against V. alginolyticus infection compared to the control group.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Jormil Revilla, Adrian Marquez, Daniel Rodriguez-Pesantes, Cristobal Dominguez-Borbor, Jenny Rodriguez, Cesar Lodeiros, Stanislaus Sonnenholzner
Article
Fisheries
Cristobal Dominguez-Borbor, Valeska Ardiles, Marissa Bermeo, Chalen Bolivar-Alvarado, Cecilia Tomala, Stanislaus Sonnenholzner, Jenny A. Rodriguez
Article
Fisheries
Cristobal Dominguez-Borbor, Aminael Sanchez-Rodriguez, Stanislaus Sonnenholzner, Jenny Rodriguez
Article
Fisheries
Thao Nguyen, Andrea Alfaro, Bonny Bayot Arroyo, Jenny Antonia Rodriguez Leon, Stanislaus Sonnenholzner
Summary: The study used a metabolomics approach to measure molecular responses of shrimp exposed to Vibrio parahaemolyticus, revealing an increase in metabolic rate, elevated TCA cycle, and gluconeogenesis activities in infected shrimp. This provides important new findings about the pathogenic mechanisms of V. parahaemolyticus invasion in shrimp at the metabolic level.
Article
Zoology
Andrea C. Alfaro, Thao Nguyen, Bonny Bayot, Jenny A. Rodriguez Leon, Cristobal Dominguez-Borbor, Stanislaus Sonnenholzner
Summary: Outbreaks of white spot syndrome virus (WSSV) have caused serious damage to penaeid shrimp aquaculture worldwide. Despite great efforts to characterize the virus, the conditions that lead to infection and the infection mechanisms, there is still a lack of understanding regarding these complex virus-host interactions, which is needed to develop consistent and effective treatment methods for WSSV. In this study, a metabolomics approach was used to compare metabolite profiles of WSSV-challenged shrimp and controlled shrimp in gills, haemolymph and hepatopancreas, revealing tissue-specific immune responses of shrimp to WSSV. The findings provide insights for the development of disease management strategies against WSSV invasion.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2021)
Article
Microbiology
Leda Restrepo, Cristobal Dominguez-Borbor, Leandro Bajana, Irma Betancourt, Jenny Rodriguez, Bonny Bayot, Alejandro Reyes
Summary: The study demonstrates the potential of probiotics in controlling pathogenic bacterial populations in shrimp's gastrointestinal tract, particularly against AHPND-causing Vibrio pathogens. However, the risk of probiotic strain becoming pathogenic when working with AHPND-causing Vibrio pathogens needs to be carefully evaluated.
Article
Biochemistry & Molecular Biology
Maria S. Pena-Carrillo, Edgar A. Pinos-Tamayo, Bruno Mendes, Cristobal Dominguez-Borbor, Carolina Proano-Bolanos, Danilo C. Miguel, Jose R. Almeida
Summary: This study evaluated the in vitro therapeutic potential of three biomimetic peptides derived from Asp49 PLA(2)s, highlighting their antimicrobial, cytotoxic, and antileishmanial activities. The results demonstrated the versatility of PLA(2)-derived synthetic peptides as potential drug candidates for public health issues.
BIOORGANIC CHEMISTRY
(2021)
Article
Fisheries
Mery Ramirez, Cristobal Dominguez-Borbor, Lizeth Salazar, Alexis Debut, Karla Vizuete, Stanislaus Sonnenholzner, Frank Alexis, Jenny Rodriguez
Summary: This study found that using a probiotic consortium with colonization capacity can effectively control AHPND and improve the protection of shrimp larvae.
Article
Fisheries
Andrea C. Alfaro, Thao Nguyen, Jenny A. Rodriguez, Bonny Bayot, Cristobal Dominguez-Borbor, Stanislaus Sonnenholzner, Awanis Azizan, Leonie Venter
Summary: Metabolomics approach using GC-MS was used to analyze metabolite profiles of whiteleg shrimp exposed to different treatments in aquaculture. Significant differences were found in haemolymph metabolite profiles of immune stimulated treatments, especially in the combination of cellulose fiber and probiotics. Potential biomarkers for health assessment in aquaculture were identified as itaconic acid and lactic acid.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Microbiology
Leandro Bajana, Irma Betancourt, Bonny Bayot
Summary: In this study, the second published genome sequence of Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) was reported. It was isolated from cultured Penaeus vannamei shrimp in Ecuador and has been associated with the runt-deformity syndrome (RDS).
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Microbiology
Alejandro Castellanos, Leda Restrepo, Leandro Bajana, Irma Betancourt, Bonny Bayot, Alejandro Reyes
Summary: AHPND is a disease causing significant losses in the shrimp farming industry, with global losses exceeding $2.6 billion. The most common etiological agent is V. parahaemolyticus strains carrying the PirAB(vp) toxin. By analyzing South American AHPND-causing V. parahaemolyticus isolates at the genomic level, it was found that they have high similarity but do not cluster with other Mexican strains, suggesting different genetic backgrounds and possible acquisition of the pVA1-type plasmid through horizontal gene transfer at different times.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Cristobal Dominguez-Borbor, Irma Betancourt, Fanny Panchana, Stanislaus Sonnenholzner, Bonny Bayot