Article
Multidisciplinary Sciences
Chancievan Thangaratnarajah, Jan Rheinberger, Cristina Paulino, Dirk J. Slotboom
Summary: Energy-coupling factor (ECF)-type transporters are small, asymmetric membrane protein complexes that import micronutrients into bacterial cells. Recent research suggests that during the transport process, protein-induced membrane deformations facilitate the toppling of the S component.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Pharmacology & Pharmacy
Yuqi Yang, Zhuo-Xun Wu, Jing-Quan Wang, Qiu-Xu Teng, Zi-Ning Lei, Sabrina Lusvarghi, Suresh V. Ambudkar, Zhe-Sheng Chen, Dong-Hua Yang
Summary: OTS964 is a potent inhibitor of TOPK that interacts with ABCG2 to affect multidrug resistance, and has an upregulating effect on ABCG2 transporter. It enhances resistance to ABCG2 substrate-drugs by inhibiting efflux function mediated by ABCG2.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Kamil Nosol, Rose Bang-Sorensen, Rossitza N. Irobalieva, Satchal K. Erramilli, Bruno Stieger, Anthony A. Kossiakoff, Kaspar P. Locher
Summary: ABCB4 is expressed in hepatocytes and plays a key role in translocating phosphatidylcholine into bile canaliculi. Cryogenic electron microscopy structures of ABCB4 reveal its specificity for phosphatidylcholine and the essential role of key residues in this process. These findings provide a structural basis for understanding bile generation and its sensitivity to azole drugs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemical Research Methods
Ryuichi Hirota, Zen-ichiro Katsuura, Naoki Momokawa, Hiroki Murakami, Satoru Watanabe, Takenori Ishida, Takeshi Ikeda, Hisakage Funabashi, Akio Kuroda
Summary: In this study, it was found that under specific conditions, a phosphite-dependent strain of Escherichia coli can generate escape mutants. The mutation occurs at position 210 of the transmembrane domain protein HtxC of the phosphorus compound transporter HtxBCDE. The replacement of a specific amino acid residue at this position can reduce the rate of escape mutant generation.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tiantian Yang, Sikandar Amanullah, Shenglong Li, Rui Cheng, Chen Zhang, Zhengxiang Zhao, Hongyu Liu, Feishi Luan, Xuezheng Wang
Summary: The genetic regulatory basis of qualitative and quantitative phenotypes of watermelon is being studied using molecular and genetic breeding approaches. In this study, mapping populations were developed and genetic linkage maps were constructed. QTLs and candidate genes related to fruit and seed traits were identified. The results provide valuable insights for marker-assisted breeding to improve the quality traits of watermelon.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kristin Oepen, Hueseyin Ozbek, Anja Schueffler, Johannes C. Liermann, Eckhard Thines, Dirk Schneider
Summary: ABC transporters, found in all kingdoms of life, transport substrates against concentration gradients across membranes. Some natural substances have been found to inhibit the activity of ABC transporters, showing potential implications for the regulation of these proteins in biological membranes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Shoji Kokubo, Shinobu Ohnuma, Megumi Murakami, Haruhisa Kikuchi, Shota Funayama, Hideyuki Suzuki, Taiki Kajiwara, Akihiro Yamamura, Hideaki Karasawa, Norihiko Sugisawa, Kosuke Ohsawa, Kuniyuki Kano, Junken Aoki, Takayuki Doi, Takeshi Naitoh, Suresh V. Ambudkar, Michiaki Unno
Summary: This study identified a novel ABCG2 inhibitor, PFC, through high-throughput screening, which significantly decreased IC50 of SN-38 in cancer cells and reversed resistance to irinotecan without causing toxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Chung-Pu Wu, Sung-Han Hsiao, Yu-Shan Wu
Summary: The overexpression of human ATP-binding cassette (ABC) transporters in cancer cells can lead to multidrug resistance (MDR), which poses a significant obstacle to chemotherapy. Currently, there are no approved drugs specifically designed to treat multidrug-resistant cancers. However, drug repurposing has emerged as a practical approach to discover effective modulators of drug transporters.
DRUG RESISTANCE UPDATES
(2023)
Article
Neurosciences
Zhongsong Xiao, Peizheng Li, Yiqing Shen, Anatol Manaenko, Wensong Yang, Peng Wang, Xinhui Li, Fangyu Liu, Peng Xie, Qi Li
Summary: Despite limited treatment options for intracerebral hemorrhage (ICH), recent evidence suggests that the ultra-early stage of ICH may have significant importance. This study investigated the metabolic changes induced by ICH in the ultra-early stage using a mouse model and identified key pathological mechanisms associated with this stage.
EXPERIMENTAL NEUROLOGY
(2023)
Review
Pharmacology & Pharmacy
Erkka Jarvinen, Feng Deng, Wilma Kiander, Alli Sinokki, Heidi Kidron, Noora Sjostedt
Summary: Glucuronidation and sulfation are common phase II metabolic reactions of drugs, resulting in inactive and safe conjugates. However, these conjugates may be the most prominent drug-related substances in human circulation and excreta. Understanding the active transport of these metabolites is crucial for predicting drug fate and ensuring safety.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Environmental Sciences
Lamia Sakouhi, Oumayma Kadri, Sirine Werghi, Marouane Ben Massoud, Oussama Kharbech, Yoshiyuki Murata, Abdelilah Chaoui
Summary: In this study, the beneficial effect of melatonin on alleviating cadmium toxicity in chickpea plants was investigated. The results showed that pretreating the seeds with melatonin improved the growth of seedlings under cadmium stress, reduced cadmium accumulation, and protected the cell membrane integrity.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Lea Girard, Niels Geudens, Brent Pauwels, Monica Hofte, Jose C. Martins, Rene De Mot
Summary: Pseudomonas lipopeptides are secondary metabolites with diverse ecological functions and biotechnological applications, and a reliable transporter gene-guided approach allows for the detection and classification of lipopeptide producers among Pseudomonas strains, providing a valuable tool for strain prioritization.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Cinthia R. Millan, Martina Francis, Nitesh Kumar Khandelwal, Valery F. Thompson, Tarjani M. Thaker, Thomas M. Tomasiak
Summary: The study reveals the important role of the GRD motif in coordinating domain rearrangements in the heterodimeric peptide transporter TmrAB, with disruption of this network leading to different functional consequences and hinting at intrinsic asymmetry in the transporter. These findings suggest a mechanism by which the GRD motifs help coordinate a transition to an outward open conformation, with each half of the transporter playing a different role in the conformational cycle of TmrAB.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ashlee M. Plummer-Medeiros, Alan T. Culbertson, Claudio L. Morales-Perez, Maofu Liao
Summary: The human ATP-binding cassette transporter ABCA1 plays a critical role in lipid homeostasis. Deleterious mutations of ABCA1 lead to sterol accumulation and are associated with various diseases. The mechanism by which ABCA1 drives lipid movement is poorly understood. In this study, a stable expression system was established for both a human cell-based sterol export assay and protein purification. The produced ABCA1 protein showed activity in sterol export and displayed enhanced ATPase activity. Cryo-EM study revealed multiple distinct conformations and a previously unknown structure of nanodisc-embedded ABCA1. This platform provides important mechanistic and structural insights for investigating ABCA1 modulators.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Microbiology
Lei Qiao, Xina Dou, Xiaofan Song, Jiajing Chang, Xiaonan Zeng, Lixu Zhu, Chunlan Xu
Summary: In this study, it was found that food-grade probiotic L. casei ATCC 393 effectively reduced selenite to SeNPs by GSH and nitrate reductase-mediated reduction pathway, providing an environmentally friendly biocatalyst for the bioremediation of Se contamination.
MICROBIOLOGY SPECTRUM
(2023)
Review
Biotechnology & Applied Microbiology
Udita Basu, Swarup K. Parida
Summary: Plants have adapted to different environments by regulating developmental factors, resulting in variations in traits that contributed to their survival. Key developmental pathways, such as CLAVATA-WUSCHEL, GID1-DELLA, LAZY1-TAC1, and TFL1-FT, play significant roles in plant architecture. Allelic variants of these regulators shaped crops during domestication. With genome-editing techniques, it is possible to manipulate these developmental pathways to customize crops for specific environments and increase yield stability.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Uday Chand Jha, Harsh Nayyar, Swarup K. Parida, R. Beena, Jiayin Pang, Kadambot H. M. Siddique
Summary: Phosphorus (P) is a crucial nutrient for plant growth, but its sources are non-renewable, raising concerns for sustainable agriculture and food security. Improving plant P-use efficiency (PUE) is necessary for intensive cropping systems, and this review explores various breeding approaches and tools to enhance PUE in grain legumes. The genetic architecture of PUE traits is complex, influenced by both genetics and the environment. Advances in genomics and functional genomics have provided insights into the underlying genetic mechanisms and pathways related to PUE, while novel breeding schemes and tools offer opportunities for designing P-use-efficient cultivars in low-P environments.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Amit Kumar Singh, Pradeep Kumar, Vishnu D. Rajput, Sunil Kumar Mishra, Kavindra Nath Tiwari, Anand Kumar Singh, Tatiana Minkina, Ajay Kumar Pandey
Summary: Hyperglycemia is the characteristic of diabetes, leading to cardiovascular disease, nephropathy, neuropathy, and retinopathy. Ethanolic novel polyherbal extract (PHE) prepared from a mixture of plant materials contains various bioactive compounds with antioxidant and anti-inflammatory activities.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Engineering, Environmental
Priya Kumari, Juhi Sharma, Anup Kumar Singh, Ajay Kumar Pandey, Farnaz Yusuf, Shashi Kumar, Naseem A. Gaur
Summary: Developing microbial chassis for synthesizing value-added compounds is an eco-friendly approach to minimize the impacts of overusing fossil fuels and harsh chemicals. This study focused on modifying the fatty acid biosynthesis pathway in a strain of Saccharomyces cerevisiae to produce fatty acid ethyl ester (FAEE) with high yield and titer. The engineered strain showed promising results, producing FAEE at a high titer and with a high percentage of monounsaturated fatty acids.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Plant Sciences
Anurag Daware, Ankit Malik, Rishi Srivastava, Durdam Das, Ranjith K. K. Ellur, Ashok K. K. Singh, Akhilesh K. K. Tyagi, Swarup K. K. Parida
Summary: The advent of the pangenome era has uncovered previously unknown genetic variation in diverse crop plants, but conventional single-reference genotyping methods often fail to capture a large portion of this variation, leading to reference bias. To overcome this, a Rice Pangenome Genotyping Array (RPGA) was developed, enabling rapid genotyping based on the entire 3K rice pangenome. This array provides a cost-effective solution and has been successfully applied in various areas, such as genome-wide association studies, population structure analysis, and marker-assisted selection.
Article
Biology
Arunima Mahto, Antima Yadav, P. V. Aswathi, Swarup K. Parida, Akhilesh K. Tyagi, Pinky Agarwal
Summary: By analyzing the transcriptomes and miRNomes of small-grained and large-grained indica rice, this study identified the crucial role of the S3 stage in enhancing grain size and discovered several transcription factor and miRNA families that influence grain size. A Domino effect model was proposed to explain the regulation of grain size. Additionally, a rice grain development database was established for easy access to the data generated in this study.
Article
Plant Sciences
Gazaldeep Kaur, Varsha Meena, Anil Kumar, Gaurav Suman, Deepshikha Tyagi, Riya Joon, Janneke Balk, Ajay Kumar Pandey
Summary: Iron (Fe) limitation induces changes in gene expression in plants, leading to iron uptake and mobilization while restricting Fe-dependent metabolism and growth. RNA-seq studies were performed on wheat plants exposed to Fe stress to understand the underlying molecular pathways and biochemical processes in this stress response. Transcriptional analysis revealed dynamic changes in gene expression patterns, particularly in membrane transporters and transcription factors related to Fe homeostasis. Comparative analysis of the Fe deficiency response at different time points revealed a core set of Fe-regulated genes, with an increased induction bias at day 8 compared to day 4. Prolonged Fe stress had compounding effects on the number and levels of gene expression. The study contributes to the understanding of Fe signaling networks in wheat and highlights the interplay of the three sub-genomes in regulating the transcriptional response.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Chemistry, Applied
Paras Sharma, Giridhar Goudar, Anil Kumar Chandragiri, R. Ananthan, K. Subhash, Anitha Chauhan, T. Longvah, Mohar Singh, Rakesh Bhardwaj, Swarup Kumar Parida, Amit Kumar Singh, Debasis Chattopadhyay, Debasis Chattopadhyay
Summary: A study evaluated the nutrient composition, anti-nutrients, and mineral composition of 38 ricebean accessions cultivated in India. The accessions showed variation in protein, dietary fiber, oligosaccharides, free sugars, resistant starch, iron, zinc, and selenium content. The study employed hierarchical clustering analysis and principal component analysis to analyze the diversity of nutrients and anti-nutrients across the ricebean accessions.
Article
Plant Sciences
Samriti Mankotia, Dhriti Singh, Kumari Monika, Muskan Kalra, Himani Meena, Varsha Meena, Ram Kishor Yadav, Ajay Kumar Pandey, Santosh B. Satbhai
Summary: Iron is essential for plants and animals, and its limitation significantly affects crop yield and human nutrition. Plants have adapted strategies to regulate iron uptake and homeostasis, as well as modify root system architecture to survive under low iron conditions. Understanding the molecular mechanism of root growth responses has implications for plant breeding.
Review
Biochemistry & Molecular Biology
Udita Basu, Swarup K. Parida
Summary: This review explores the developmental similarities and peculiarities in chickpea and its close relatives pea and Medicago. Key features in chickpea which have potential in yield enhancement have been discussed. However, the developmental particularities and its potential in reforming the yield and nutritional value remain largely unexplored, despite advancements in next-generation sequencing and genomics tools.
PLANT MOLECULAR BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Shahnewaz Ali, Yaqub Jonmohamadi, Davide Fontanarosa, Ross Crawford, Ajay K. Pandey
Summary: Minimally invasive surgery (MIS) offers advantages such as minimum blood loss and quick recovery time, but lacks tactile feedback and sufficient visualization of the surgical site. This article introduces an online preprocessing framework that addresses the visualization challenges associated with MIS. The proposed method provides a clean and sharp image in the standard RGB color space from noisy, blurred, and raw inputs in a single preprocessing step, outperforming existing solutions in high-level vision tasks with reduced computation time.
SCIENTIFIC REPORTS
(2023)
Article
Plant Sciences
Thippeswamy Danakumara, Tapan Kumar, Neeraj Kumar, Basavanagouda Siddanagouda Patil, Chellapilla Bharadwaj, Umashankar Patel, Nilesh Joshi, Shayla Bindra, Shailesh Tripathi, Rajeev Kumar Varshney, Sushil Kumar Chaturvedi
Summary: Identifying a suitable production environment and understanding the genotype by environmental interactions are crucial for achieving yield stability in chickpeas. Different models were used to analyze the interactions and stability of genotypes. Stabile genotypes can serve as parental lines for heat-stress tolerance breeding programs.
Proceedings Paper
Engineering, Electrical & Electronic
Ajay Kumar Pandey, Surya Kumar Pathak
Summary: This paper presents a simple approach to switch the radiation beam of a Cylindrical Dielectric Resonator Antenna (CDRA) in two directions for S-band applications using a helical wire. The proposed antenna can alter its radiation pattern to focus energy in two specified directions while reducing gain in other undesired directions, without changing the antenna impedance bandwidth.
2023 INTERNATIONAL MICROWAVE AND ANTENNA SYMPOSIUM, IMAS
(2023)
Article
Agriculture, Multidisciplinary
Nilesh Joshi, Sneha Priya Pappula Reddy, Neeraj Kumar, Chellapilla Bharadwaj, Kumar Tapan, B. S. Patil, Pradeep Kumar Jain, M. S. Nimmy, Manish Roorkiwal, Preeti Verma, Rajeev K. Varshney, Kadambot H. Siddique, K. Sudhir
Summary: This study aimed to identify salt stress tolerant genotypes of chickpea at the seedling stage. Through screening 50 chickpea accessions, it was found that root traits were better indicators of salt stress tolerance. The most salt tolerant and salt sensitive genotypes were ICCV10 and ILC5595, respectively.
CROP & PASTURE SCIENCE
(2023)
