Article
Microbiology
Fang Yuan, Shouliang Yin, Yang Xu, Lijun Xiang, Haiyan Wang, Zilong Li, Keqiang Fan, Guohui Pan
Summary: Catalases in bacteria have a crucial role in defense against oxidative stress and are involved in various cellular processes. The study revealed the diversity and distribution of catalases among bacterial genomes, highlighting their importance in secondary metabolite biosynthesis. Further investigation into the physiological roles of catalases may aid in enhancing the yield of valuable secondary metabolites in engineered bacteria.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Dian Ding, Jing-Xiang Wu, Xinli Duan, Songling Ma, Lipeng Lai, Lei Chen
Summary: ATP-sensitive potassium channels (K-ATP) composed of Kir6 and SUR subunits convert the metabolic status of the cell into electrical signals. The authors present cryo-EM structures of SUR2A and SUR2B subunits in complex with two vasodilators, P1075 and levcromakalim, uncovering the mechanisms of these drugs.
NATURE COMMUNICATIONS
(2022)
Article
Biophysics
Elif Esen, Simge Ozturk, H. Deniz Ertugrul Uygun, M. Nalan Demir
Summary: Catalase is a widely distributed metalloenzyme that catalyzes the conversion of hydrogen peroxide. A novel affinity sorbent was developed for determining and eliminating catalase, with high adsorption capacity. Immobilized catalase in the sorbent showed high operational and storage stability, indicating increased substrate affinity through immobilization.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Cardiac & Cardiovascular Systems
Meili Wang, Xiaochen Yin, Shuanglei Li, Xi Zhang, Ming Yi, Chunyu He, Xiaoyue Li, Wei Wang, Suli Zhang, Huirong Liu
Summary: In this study, we provide compelling evidence that dysfunction of the BK channel alpha-subunit mediates AT1-AAs-induced mesenteric artery remodeling. Preservation of BK channel activity may serve as a potential strategy for the treatment of AT1-AAs-induced maladaptive resistance artery remodeling.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Pharmacology & Pharmacy
Mikhail Dubinin, Vlada S. Starinets, Natalia Belosludtseva, Irina B. Mikheeva, Yuliya A. Chelyadnikova, Anastasia D. Igoshkina, Aliya B. Vafina, Alexander A. Vedernikov, Konstantin N. Belosludtsev
Summary: This study shows that mitochondrial dysfunction and potassium imbalance play a role in the development of Duchenne muscular dystrophy. Activating the mitoBK(Ca) channel can improve mitochondrial function and potassium homeostasis, leading to a reduction in muscle degeneration and fibrosis.
Article
Chemistry, Analytical
Tetyana Prokopiv, Nataliya Stasyuk, Mykhailo Gonchar
Summary: Nanomaterials with artificial enzyme-like catalytic activity (nanozymes) have great potential for various applications. This study demonstrates the successful substitution of natural enzyme activity by platinum and platinum-gold nanoparticles in yeast cells. This finding opens up new possibilities in screening for nanozymes and using modified cells as sensitive elements in biosensors.
Article
Chemistry, Multidisciplinary
Meng-Jie Zhang, Meng-Yun Liang, Shao-Chen Yang, Xian-Bin Ma, Shu-Cheng Wan, Qi-Chao Yang, Shuo Wang, Zhigang Xu, Zhi-Jun Sun
Summary: Glutathione-responsive nanogels (CDNPs) crosslinked with crosslinker DBHD incorporating BRAF inhibitor dabrafenib and COX2 inhibitor celecoxib were synthesized. The CDNPs can efficiently induce tumor cell pyroptosis and activate strong antitumor immunity. Furthermore, CDNPs combined with alpha PD-1 antibody significantly suppressed tumor growth and prolonged survival in a melanoma mouse model.
CHEMICAL COMMUNICATIONS
(2023)
Article
Spectroscopy
Honnur Krishna, Krishnegowda Avinash, Anantharaman Shivakumar, Naef Ghllab Saeed Al-tayar, Ashwinee Kumar Shrestha
Summary: A novel method for quantifying catalase activity in blood samples was developed using a chromogenic reaction with pyrocatechol and 4-aminoantipyrine in tris buffer. The method showed good linearity, accuracy, and precision, making it suitable for measuring CAT activity in biological samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Review
Chemistry, Multidisciplinary
Renzeng Chen, Xianheng Chen, Yuanbo Wang, Bo Wang
Summary: Metal-organic frameworks (MOFs) have advantages in terms of high porosity, crystallinity, and structural diversity, allowing them to imitate bioactive agents and meet biological demands. MOFs can act as bioinspired vectors for targeted drug delivery by emulating or integrating unique biostructures with targeting abilities. They also have potential for efficient biocatalysis by mimicking or immobilizing natural enzymes, as well as biosensing by introducing reactive biological agents or responsive bioinspired ligands and metal ions. Challenges, insights, and future directions associated with bioinspired MOFs as biomedical, biocatalytic, or biosensing agents are discussed.
TRENDS IN CHEMISTRY
(2023)
Article
Materials Science, Biomaterials
Tapan Dey, Netra Hiremath, Vishav Kant, Rakesh K. Sharma, Raviraj Vankayala, Saikat Dutta
Summary: In this study, the impact of embedding oxidoreductase enzymes in metal-organic frameworks (ZIF-8 and ZIF-90) on cellular metabolic activity was investigated. The results showed that the embedding pattern of catalase in ZIF-8 and ZIF-90 depended on the surface chemistry of the enzyme. The MTT assay revealed enhanced cell viability in A549 lung cancer cells when treated with Cat@ZIF-8, compared to Cat@ZIF-90.
BIOMATERIALS SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Andrej Poljovka, Milos Musil, David Bednar, Katarina Chovanova, Vladena Bauerova-Hlinkova, Jana Bellova, Lenka Kohutova, Peter Barath, Marcel Zamocky
Summary: The study found that the catalase-peroxidases from thermophilic and mesophilic fungi have similar active center structures and peroxidase activity, while the thermophilic variant CthedisKatG showed enhanced manganese peroxidase activity at higher temperatures. Two constructed mutations completely abolished catalatic activity and altered the peroxidase activity, suggesting the potential biotech applications of bifunctional heme enzymes with variable reactivity.
Article
Chemistry, Analytical
Antonio Clemente, Alejandra Alba-Patino, Giulia Santopolo, Enrique Baron, Estrella Rojo-Molinero, Antonio Oliver, Jon Perez-Barcena, Paz Merino de Cos, Maria Aranda, Alberto del Castillo, Antonia Socias, Marcio Borges, Roberto de la Rica
Summary: The study introduced an enzymatic method for liquefying respiratory samples, which resulted in better liquefaction compared to the traditional DTT method and led to higher ELISA signals. Lung IL-6 levels obtained with the enzymatic procedure were negatively correlated with the oxygenation index and the time of mechanical ventilation.
Article
Biochemistry & Molecular Biology
Inga Staneviciene, Jurgita Sulinskiene, Ilona Sadauskiene, Arunas Liekis, Ausrine Ruzgaite, Rima Naginiene, Dale Baranauskiene, Vaida Simakauskiene, Raulas Krusnauskas, Dale Viezeliene
Summary: Selenium plays a crucial role in maintaining normal brain function and modulating cellular redox status and iron homeostasis. This study investigated the effects of inorganic selenium excess on oxidative stress and iron homeostasis in the brain and liver of mice. The results showed that selenium accumulated in both organs and disrupted iron levels. Selenium also induced oxidative damage in the liver, but its effects on the brain were dose-dependent, with lower doses providing antioxidant activity and higher doses activating catalase expression to maintain lipid peroxidation levels.
Article
Chemistry, Multidisciplinary
Muhammad Yasir, Jinyoung Park, Eun-Taek Han, Won Sun Park, Jin-Hee Han, Yong-Soo Kwon, Hee-Jae Lee, Wanjoo Chun
Summary: Artificial intelligence algorithms are increasingly used in drug development for their efficiency and effectiveness. Deep-learning-based drug repurposing can identify novel therapeutic applications for drugs. In this study, a trained deep-learning model was used to screen an FDA-approved drug library and predict COX-2 inhibitory potential.
Article
Cell Biology
Raghbendra Kumar Dutta, Joon No Lee, Yunash Maharjan, Channy Park, Seong-Kyu Choe, Ye-Shih Ho, Hyug Moo Kwon, Raekil Park
Summary: This study investigated the role of catalase and lysosomal function in catalase-knockout mice, revealing that mature adult catalase-KO mice exhibited accelerated aging phenotypes compared to wild-type mice, with leaky lysosomes, impaired autophagy, and mTORC1 activation.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Tomasz Antonowski, Adam Osowski, Damian Szczesny, Joanna Szablinska-Piernik, Jerzy Juskiewicz, Leslaw Lahuta, Andrzej Rynkiewicz, Joanna Wojtkiewicz
Summary: This study investigated the concentration change of myo-inositol solution in the serum of Wistar rats after oral administration. The results showed that the myo-inositol concentration reached its peak in the first hour and then rapidly declined. However, even after 24 hours, the concentration remained higher than before administration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Bartosz Fotschki, Katarzyna Ognik, Joanna Fotschki, Dorota Napiorkowska, Ewelina Cholewinska, Magdalena Krauze, Jerzy Juskiewicz
Summary: The study investigated the effects of chromium supplementation and switching dietary habits on lipid metabolism, inflammation, and oxidative stress in Wistar rats. The combination of chromium nanoparticles and dietary changes showed the strongest reduction in fat content and cholesterol in the liver. This combination also had favorable antioxidative effects. Supplementation with chromium nanoparticles alone also improved lipid metabolism and oxidative stress, although to a lesser extent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Bartosz Fotschki, Ewelina Cholewinska, Katarzyna Ognik, Michal Sojka, Joanna Milala, Joanna Fotschki, Wieslaw Wiczkowski, Jerzy Juskiewicz
Summary: The required amount of berry polyphenols for health benefits is difficult to achieve through fresh fruit consumption, suggesting polyphenol-rich extracts as a dietary alternative. This study fed rats high-fat diets supplemented with 0.1 or 0.3% raspberry polyphenols and found that the higher dose of extract had more noticeable health-promoting effects, including reduced white adipose tissue weight, hepatic triglyceride content, and plasma IL-6 concentration, as well as increased acetic acid concentration in the cecal digesta. The enhanced content of ellagitannin and anthocyanin metabolites in the blood plasma of rats administered the high dose extract may partially explain these effects.
Article
Nutrition & Dietetics
Natalia Drabinska, Jerzy Juskiewicz, Wieslaw Wiczkowski
Summary: The interest in the application of the ketogenic diet (KD) for obesity management is growing. This study found that calorie-restricted KD can reduce oxidative stress without compromising the haematological status. However, caution is needed to control adiposity, glucose level and liver health.
Article
Nutrition & Dietetics
Michal Majewski, Leszek Gromadzinski, Ewelina Cholewinska, Katarzyna Ognik, Bartosz Fotschki, Jerzy Juskiewicz
Summary: This study aimed to evaluate the effects of a high-fat-low-fiber diet on the cardiovascular system's response to chromium picolinate and chromium nanoparticles, and found that in the intervention group after 18 weeks on the F diet, both Cr-Pic and Cr-NPs could function as pro-oxidant agents, leading to oxidative stress.
Review
Biochemistry & Molecular Biology
Joanna Fotschki, Anna M. M. Ogrodowczyk, Barbara Wroblewska, Jerzy Juskiewicz
Summary: The vegetable processing industry generates large amounts of by-products that are susceptible to microbial degradation. Inadequate management leads to the loss of valuable compounds in vegetable by-products. Scientists are exploring the possibility of reusing discarded biomass to create higher value products. The by-products contain bioactive compounds with health-promoting properties. This review emphasizes the importance of these by-products in promoting health through their impact on the microbiota, immune system, and gut milieu.
Article
Nutrition & Dietetics
Ewelina Cholewinska, Aleksandra Marzec, Przemyslaw Solek, Bartosz Fotschki, Piotr Listos, Katarzyna Ognik, Jerzy Juskiewicz
Summary: The aim of this study was to investigate the effects of different types of dietary fibre (cellulose, inulin, pectin, and psyllium) in combination with recommended or enhanced levels of CuNPs on selected biological parameters of intestinal integrity in rats. The results showed that replacing CuCO3 with CuNPs in the diet reduced lactic acid and apoptosis markers levels in the small intestine, but it also increased DNA oxidation. Inulin had the most beneficial effect on DNA repair mechanisms, while pectin had the highest ability to inhibit inflammatory processes and apoptotic cell death in the small intestine. These findings suggest that dietary fibre supplementation can protect the small intestine against oxidative effects of CuNPs by enhancing the intestinal barrier.
Article
Biochemistry & Molecular Biology
Monika Cendrowska-Pinkosz, Magdalena Krauze, Jerzy Juskiewicz, Bartosz Fotschki, Katarzyna Ognik
Summary: The aim of this study is to evaluate the effect of different forms and dosages of copper on neurodegenerative markers in the brain and jejunum. The experiment involved feeding male Wistar rats a rat diet with two CuCO3 dosages and two CuNP dosages. Nanoparticles reduced the level of GAChE, increased the levels of AChE and LRP1, and decreased β-amyloid and Tau protein in both the brain and intestine of rats. The highest levels of beneficial markers and lowest levels of toxic markers were observed in rats receiving a standard dose of Cu.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Grzegorz Chmielewski, Michal S. Majewski, Jakub Kuna, Mateusz Mikiewicz, Magdalena Krajewska-Wlodarczyk
Summary: Fatigue is a common symptom in various rheumatic diseases, affecting patients' quality of life and exacerbating disability. Its nature involves multiple factors, including physiological, psychological, and social aspects. Despite its significance, fatigue is often underestimated in clinical practice. Comprehensive assessment of the subjective components of fatigue is crucial for effective management of this symptom in inflammatory joint diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Michal Majewski, Leszek Gromadzinski, Ewelina Cholewinska, Katarzyna Ognik, Bartosz Fotschki, Jerzy Juskiewicz
Summary: This study aimed to investigate the effects of dietary fiber supplementation with different types of nanoparticles on the cardiovascular system in rats. The results showed that compared to pectin, inulin and psyllium supplementation with a standard dose of copper nanoparticles improved antioxidant capacity and reduced heart malondialdehyde levels. Furthermore, with an enhanced dose of copper nanoparticles, inulin and psyllium enhanced vasodilation through up-regulation of COX-2-derived vasodilator prostanoids.
Article
Nutrition & Dietetics
Jerzy Juskiewicz, Katarzyna Ognik, Joanna Fotschki, Dorota Napiorkowska, Ewelina Cholewinska, Katarzyna Grzelak-Blaszczyk, Magdalena Krauze, Bartosz Fotschki
Summary: This study assessed the changes in faecal microbial activity in obese rats fed high-fat or low-fat diets supplemented with different forms of chromium. The results showed that switching from a high-fat to a low-fat diet had beneficial effects, and supplementing with chromium nanoparticles further modulated microbial activity.
Article
Nutrition & Dietetics
Bartosz Fotschki, Michal Sojka, Monika Kosmala, Jerzy Juskiewicz
Summary: Previous studies have shown that dietary supplementation with prebiotic fructooligosaccharides (FOSs) and polyphenols can alleviate disorders related to the early stage of nonalcoholic fatty liver disease (NAFLD). This experiment aimed to investigate whether raspberry polyphenols together with FOSs can regulate advanced-stage NAFLD in genetically obese Zucker rats. The results showed that the addition of FOSs and raspberry polyphenolic extract to the diet reduced liver fat accumulation, improved antioxidant potential, lowered plasma enzyme activities, and increased insulin sensitivity. These effects may be attributed to the upregulation of specific receptors in the liver responsible for regulating lipid metabolism and glucose and bile acid synthesis.
Article
Nutrition & Dietetics
Bartosz Fotschki, Katarzyna Ognik, Ewelina Cholewinska, Katarzyna Grzelak-Blaszczyk, Kamil Myszczynski, Magdalena Krauze, Jerzy Juskiewicz
Summary: This study aimed to investigate the effects of supplementation with chromium nanoparticles (Cr-NPs) on the gut microenvironment in rats fed a high-fat diet. The results showed that Cr-NPs mainly acted by altering the activity of gut microbiota, leading to decreased concentration of short-chain fatty acids and increased level of cecal lithocholic acid. Switching to a low-fat diet had a more significant impact on obesity-related disorders, improving microbial activity, composition, and the production of short-chain fatty acids.
Review
Nutrition & Dietetics
Klaudia Kitala, Damian Tanski, Janusz Godlewski, Magdalena Krajewska-Wlodarczyk, Leszek Gromadzinski, Michal Majewski
Summary: Copper and zinc are essential micronutrients that control various cellular pathways and enzyme activity. Imbalances in their concentrations can lead to cardiovascular dysfunction. Dietary intake of excessive zinc can cause copper deficiency. Copper plays a role in vascular remodeling, while zinc is important in the development of heart damage. Metal nanoparticles and their effects on vascular health require further investigation.
Article
Agriculture, Dairy & Animal Science
Zuzanna Calyniuk, Ewelina Cholewinska, Pawel Konieczka, Katarzyna Ognik, Dariusz Mikulski, Jan Jankowski
Summary: Increasing the amount of lysine in turkey diet by 10% relative to NRC recommendations did not enhance growth performance, but had beneficial effects on metabolism and antioxidant status. An arginine level of 95% lysine can be used in a diet for turkeys containing 10% more lysine than the level recommended by NRC (1994).
ANNALS OF ANIMAL SCIENCE
(2022)