Journal
ANALYTICAL METHODS
Volume 8, Issue 10, Pages 2138-2143Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6ay00122j
Keywords
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Funding
- Jiangsu Province's Clinical Science & Technology Special Project [BL2012061]
- National Natural Science Foundation of China [21405176, 21475151, 31300704, 21275161]
- Jiangsu Provincial Natural Science Foundation [BK20151445]
- China Postdoctoral Science Foundation [2012M512179, 2013T60962]
- Fundamental Research Funds for the Central Universities [2015ZD008]
- Open Project Program of MOE Key Laboratory of Drug Quality Control and Pharmacovigilance [DQCP2015MS02]
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Non-invasive detection of trisomy 21 is a safe and effective way for prenatal diagnosis. Although using next generation sequencing technology can achieve non-invasive detection of chromosomal abnormalities, a more convenient and cost-effective method is preferable for routine clinical applications. Here, we proposed a novel method for the detection of trisomy 21 by accurately quantifying the slightly increased amount of chromosome 21 in cell-free DNA from maternal plasma using digital PCR. The segmental duplication fragments on chromosome 21 and chromosome 1 were employed as the detection target of digital PCR. As low as 10% cell-free fetal DNA of trisomy 21 fetus in maternal cell-free DNA was successfully detected. Three trisomy 21 samples were unambiguously picked up from 15 clinical samples, indicating that our method has the potential for non-invasive diagnosis of trisomy 21.
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