Article
Agronomy
Yonglei Zhang, Runnan Yu, Jing Tang, Liheng Du, Yihan Wang, Jiatao Wang, Linsu Liu, Shanshan Gao, Bin Li
Summary: Three insect cytochrome P450 monooxygenase genes (TcCYP4Q3, TcCYP4Q5, and TcCYP4Q7) in Tribolium castaneum are regulated by the CncC signaling pathway and play a role in the susceptibility of the beetles to eugenol, a phytochemical. These findings have implications for the development of novel therapeutics to control pests.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agronomy
Janin Rosner, Johanne Tietmeyer, Hans Merzendorfer
Summary: The study focused on the potential functions of TcABCG and TcABCH genes in insecticide detoxification. Upregulation of some genes after insecticide treatment was found, but silencing these genes did not significantly increase susceptibility to insecticides, indicating an unspecific stress response rather than direct involvement in detoxification processes.
PEST MANAGEMENT SCIENCE
(2021)
Article
Entomology
Janin Roesner, Hans Merzendorfer
Summary: ABC transporters are suggested to play a role in insecticide detoxification, with specific TcABCA-C genes being upregulated in response to insecticide exposure. Inhibition of ABC transporters enhances the toxicity of certain insecticides, while silencing of specific TcABCC genes increases susceptibility to particular insecticides. These findings highlight the potential involvement of ABC transporters in detoxification processes in insects.
Article
Agronomy
Mei Bai, Zi-Ling Liu, Yu-Yu Zhou, Qiu-Xuan Xu, Tong-Xian Liu, Hong-Gang Tian
Summary: Our study evaluated the effects of different dsRNA storage conditions on RNAi efficiency. The results showed that the dsRNA molecule was highly stable under typical cryopreservation temperatures and multiple freeze-thaw cycles, and there were no significant differences in RNAi efficiency. Surprisingly, dsRNA remained stable and induced an efficient RNAi response even under refrigerated storage conditions.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Kenji Shimomura, Hinoki Oikawa, Maaya Hasobe, Nobuhiro Suzuki, Shunsuke Yajima, Motohiro Tomizawa
Summary: Beetles are highly destructive pests of agriculture and stored products. Researchers have explored alternatives to pesticides for more sustainable pest management, focusing on insect transient receptor potential channels. The repellent activities of l-menthol and menthoxypropanediol for the red flour beetle Tribolium castaneum are mediated by TcTRPM, suggesting that contact repellency may be more significant for repelling beetles than olfactory responses.
PEST MANAGEMENT SCIENCE
(2021)
Article
Entomology
Yonglei Zhang, Jiahao Zhang, Dongyu Li, Haidi Sun, Ruixue Lu, Se Yin, Xinlong Guo, Shanshan Gao
Summary: In this study, three TcAOX genes (TcAOX1, TcAOX2, TcAOX3) from Tribolium castaneum genome were identified and characterized. The results showed that TcAOX1 is involved in the degradation of chemical signals, while TcAOX2 and TcAOX3 are associated with plant toxicant detoxification. Knockdown of TcAOX1 led to a reduction in female egg-laying, suggesting its role in fecundity.
BULLETIN OF ENTOMOLOGICAL RESEARCH
(2022)
Article
Genetics & Heredity
Juanjuan Liu, Shanshan Gao, Luting Wei, Wenfeng Xiong, Yaoyao Lu, Xiaowen Song, Yonglei Zhang, Han Gao, Bin Li
Summary: This study investigates the functions of choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT) in insect development, reproduction, and insecticide susceptibility. The researchers found that RNA interference of Chat and vacht in Tribolium castaneum resulted in decreased acetylcholine content and disrupted larval development and pupal eclosion. Knockdown of these enzymes also reduced fecundity and egg hatchability. Additionally, the researchers observed that the transcript levels of chat and vacht increased after treatment with carbofuran or dichlorvos, and reduction of chat or vacht decreased the resistance to these insecticides.
Article
Biochemistry & Molecular Biology
Shanshan Gao, Ruixue Lu, Yonglei Zhang, Haidi Sun, Siying Li, Kunpeng Zhang, Ruimin Li
Summary: The study found that the OBP gene OBPC12 of Tribolium castaneum may be involved in the insect's defense response to exogenous toxic substances, leading to a decrease in sensitivity to eugenol; The expression pattern of TcOBPC12 in different tissues and developmental stages varies, indicating its potential important role in larvae absorbing exogenous toxic substances.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Entomology
Waheed A. A. Sayed, Reda S. Hassan, Thanaa M. Sileem, Birgit A. Rumpold
Summary: Radio frequency plasma (RFP) is a reliable and cost-effective method for controlling insect pests in stored food products. The sensitivity of Tribolium castaneum to RFP was tested using argon (Ar) and helium (He) gases, with He RFP being more efficient. The optimal exposure time for complete mortality of all stages was found to be 90 seconds with He RFP.
JOURNAL OF PEST SCIENCE
(2021)
Article
Entomology
Shanshan Gao, Shumei Liu, Tian Gao, Xinyi Zhang, Zhuangkun Huo, Kunpeng Zhang
Summary: The study reveals that Vesicular acetylcholine transporter (vacht) plays a crucial role in the metamorphosis, reproduction, and insecticide susceptibility of Tribolium castaneum. Transcriptome profiling after Tcvacht downregulation through RNAi in T. castaneum larvae identified 479 differentially expressed genes (DEGs), including those involved in detoxification processes, immune pathways, and metabolic genes. Knockdown of Tcvacht expression resulted in decreased expression of enzymes involved in detoxification and increased susceptibility to insecticides. These findings provide valuable insights into the biological processes influenced by Tcvacht and potential strategies for biological control of T. castaneum.
INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Runnan Yu, Wenjing Zhang, Yanxiao Li, Jing Tang, KumChol Kim, Bin Li
Summary: ALKBH4 plays a crucial role in epigenetic regulation in mammals by repairing alkyl lesions, but its function in insects is not well understood. In this study, the TcALKBH4 gene was cloned and characterized in Tribolium castaneum. The expression pattern of TcALKBH4 showed high expression in early embryos and early pupae, and in the adult testis and ovary. RNA interference targeting TcALKBH4 at different developmental stages resulted in distinct phenotypes, including developmental failure in larvae, reduced food intake, and fertility deficiency in adults. However, TcALKBH4 RNAi did not affect 6mA levels in vivo. qRT-PCR analysis suggested that TcALKBH4 may mediate larval development through the 20E signaling pathway, and the fertility of female and male adults may be regulated by vitellogenesis and JH signaling pathways, respectively. These findings provide new insights into the potential function of ALKBH4 in insects.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xu Shen, Yingchuan Peng, Huifang Song, Jinda Wang, Jun Zhao, Peian Tang, Zhaojun Han, Kangxu Wang
Summary: This study investigated the competitive inhibition characteristics of different double-stranded RNAs on insect pests' RNAi responses, and provided solutions. The results showed that conjugating different dsRNA fragments into a single molecule and utilizing carbon quantum dots for dsRNA delivery were efficient methods for knocking down multiple target genes simultaneously. In vitro assays revealed the accumulation speed of dsRNA in midgut tissues varied with the amount of dsRNA treated.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kun Qian, Yi Yang, Changyin Zhou, Haoyu Zhang, Nan Zhang, Xiangkun Meng, Jianjun Wang
Summary: In this study, two putative sodium channel genes, TcNav1 and TcNav2, were cloned and characterized in Tribolium castaneum, encoding proteins with amino acid similarities to Drosophila para. Knockdown of TcNav1 and TcNav2 adversely affected adult emergence and decreased sensitivity to insecticides. These findings suggest that duplication of sodium channel genes in T. castaneum has led to diversified developmental and toxicological functions.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xin Xu, Xiangkun Meng, Nan Zhang, Heng Jiang, Huichen Ge, Kun Qian, Jianjun Wang
Summary: The study cloned a cytosolic sulfotransferase TcSULT1 from the red flour beetle, Tribolium castaneum, which is highly expressed in the head, epidermis, and malpighian tube, and is upregulated after exposure to deltamethrin. The transcription factor TcCncC was found to regulate the expression of TcSULT1.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Entomology
Shanshan Gao, Xinlong Guo, Shumei Liu, Siying Li, Jiahao Zhang, Shuang Xue, Qingbo Tang, Kunpeng Zhang, Ruimin Li
Summary: This study identified a P450 gene (CYP6BQ8) from Tribolium castaneum and investigated its expression profile and potential role in the detoxification of terpinen-4-ol. The results showed that TcCYP6BQ8 expression was higher in early- and late-larval stages and mainly expressed in the head and integument of larvae and adults. RNA interference against TcCYP6BQ8 increased terpinen-4-ol-induced larval mortality, suggesting its involvement in T. castaneum's metabolism of terpinen-4-ol.
BULLETIN OF ENTOMOLOGICAL RESEARCH
(2023)
Article
Genetics & Heredity
Jia Xie, Ming Sang, Xiaowen Song, Sisi Zhang, Donghun Kim, Jan A. Veenstra, Yoonseong Park, Bin Li
Article
Biochemistry & Molecular Biology
Xiaowen Song, Qisheng Zhong, Guifang Peng, Yanhao Ji, Yuemei Zhang, Jing Tang, Jia Xie, Jingxiu Bi, Fan Feng, Bin Li
BIOCHEMICAL JOURNAL
(2020)
Article
Agronomy
Jingxiu Bi, Mingxiao Ning, Jia Li, Ping Zhang, Lumen Wang, Shi Xu, Yi Zhong, Ziyi Wang, Qisheng Song, Bin Li
PEST MANAGEMENT SCIENCE
(2020)
Article
Entomology
Jia Xie, Guifang Peng, Miao Wang, Qisheng Zhong, Xiaowen Song, Jingxiu Bi, Jing Tang, Fan Feng, Han Gao, Bin Li
Summary: In this study, a gene called TcCPR69 was discovered in Tribolium castaneum that is involved in cuticle formation and chitin accumulation during the growth and metamorphosis of beetles. Knocking down TcCPR69 disrupted beetle development and decreased the expression of key genes related to chitin metabolism.
Article
Biochemistry & Molecular Biology
Yonglei Zhang, Shanshan Gao, Ping Zhang, Haidi Sun, Ruixue Lu, Runnan Yu, Yanxiao Li, Kunpeng Zhang, Bin Li
Summary: This study elucidates the insecticidal mechanisms of eugenol on Tribolium castaneum and provides insights into its basis. Eugenol inhibits certain enzymes and disrupts metabolism and detoxification processes, leading to paralysis or death of the beetles. Additionally, specific genes are involved in T. castaneum's response to eugenol exposure and metabolic detoxification.
MOLECULAR GENETICS AND GENOMICS
(2022)
Article
Agronomy
Yonglei Zhang, Runnan Yu, Jing Tang, Liheng Du, Yihan Wang, Jiatao Wang, Linsu Liu, Shanshan Gao, Bin Li
Summary: Three insect cytochrome P450 monooxygenase genes (TcCYP4Q3, TcCYP4Q5, and TcCYP4Q7) in Tribolium castaneum are regulated by the CncC signaling pathway and play a role in the susceptibility of the beetles to eugenol, a phytochemical. These findings have implications for the development of novel therapeutics to control pests.
PEST MANAGEMENT SCIENCE
(2022)
Article
Entomology
Han Gao, Yanxiao Li, Hui Zhang, Suisui Wang, Fan Feng, Jing Tang, Bin Li
Summary: This study identifies the diversity of neuropeptide systems and receptors in the bug Daktulosphaira vitifoliae. The differences in these systems compared to other insect orders provide a foundation for developing management strategies targeting this bug.
Article
Biochemistry & Molecular Biology
KumChol Kim, XiaoWen Song, RunNan Yu, YongLei Zhang, Han Gao, SuiSui Wang, Bin Li
Summary: This study identified and cloned a novel GSTe2 from Tribolium castaneum, which plays an important role in insect metamorphosis in addition to detoxification of xenobiotic substances.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Chengjun Li, Youwei Wang, Runting Ge, Ling Zhang, Huanyu Du, Jiangyan Zhang, Bin Li, Keping Chen
Summary: This study provides comprehensive information on the expression pattern and function of eIF6 in insects. eIF6 is highly expressed in eggs and early adults, with the highest expression in the gut of late-stage adults. Knockdown of eIF6 leads to precocious pupation and eclosion, impaired ovary and testis development, and complete repression of egg production. These findings highlight the importance of eIF6 in metamorphosis and reproduction.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Entomology
Yonglei Zhang, Huayi Ai, Yihan Wang, Ping Zhang, Liheng Du, Jiatao Wang, Suisui Wang, Han Gao, Bin Li
Summary: Accumulating evidence suggests that pattern recognition receptor C-type lectins (CTLs) play important roles in pathogen recognition. This study focused on TcCTL14, a CTL protein found in Tribolium castaneum, and investigated its biological function. The results showed that TcCTL14 was highly expressed in late pupae and the central nervous system, and its expression increased after exposure to Escherichia coli and Staphylococcus aureus. TcCTL14 was found to bind to lipopolysaccharide and peptidoglycan, and could agglutinate different bacteria in a calcium-dependent manner. Knockdown of TcCTL14 resulted in downregulation of Toll/IMD pathway transcription factors and antimicrobial peptides, as well as suppressed metamorphosis, reduced fecundity, and delayed embryogenesis of T. castaneum. Moreover, TcCTL14 was found to be involved in metamorphosis and fecundity through the 20-hydroxyecdysone and vitellogenin signaling pathways, respectively. These findings contribute to our understanding of PRR CTL function in insects and provide new insights for pest control strategies.
Article
Biochemistry & Molecular Biology
Chengjun Li, Wei Wu, Jing Tang, Fan Feng, Peng Chen, Bin Li
Summary: A study identified 883 miRNAs in Tribolium castaneum, with 102 miRNAs exhibiting stage-specific expression patterns. Knockdown of five differentially expressed miRNAs led to defects in metamorphosis and wing development. This study provides important insights into the roles of miRNAs in insect growth and development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Suisui Wang, Huayi Ai, Yonglei Zhang, Jingxiu Bi, Han Gao, Peng Chen, Bin Li
Summary: TcCTL15 is a transmembrane C-type lectin X involved in immune defense, cell adhesion, and developmental regulation. It is highly expressed in different developmental stages and tissues of Tribolium castaneum. TcCTL15 can recognize bacteria through binding PAMPs and has agglutinating activity against certain bacteria in the presence of Ca2+.
Article
Biochemistry & Molecular Biology
Runnan Yu, Wenjing Zhang, Yanxiao Li, Jing Tang, KumChol Kim, Bin Li
Summary: ALKBH4 plays a crucial role in epigenetic regulation in mammals by repairing alkyl lesions, but its function in insects is not well understood. In this study, the TcALKBH4 gene was cloned and characterized in Tribolium castaneum. The expression pattern of TcALKBH4 showed high expression in early embryos and early pupae, and in the adult testis and ovary. RNA interference targeting TcALKBH4 at different developmental stages resulted in distinct phenotypes, including developmental failure in larvae, reduced food intake, and fertility deficiency in adults. However, TcALKBH4 RNAi did not affect 6mA levels in vivo. qRT-PCR analysis suggested that TcALKBH4 may mediate larval development through the 20E signaling pathway, and the fertility of female and male adults may be regulated by vitellogenesis and JH signaling pathways, respectively. These findings provide new insights into the potential function of ALKBH4 in insects.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jingxiu Bi, Yutao Wang, Rui Gao, Pingxiang Liu, Yuying Jiang, Lei Gao, Bin Li, Qisheng Song, Mingxiao Ning
Summary: TcCTL16 plays a role in the developmental regulation of early larvae and the regulation of antimicrobial peptide (AMP) expression in late larvae. Its deficiency leads to abnormal development and decreased expression of muscle-regulation-related genes. TcCTL16 is mainly distributed in the hemolymph and its expression is upregulated after bacterial challenges.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)