Journal
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
Volume 2019, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2019/8639791
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Funding
- National Natural Science Foundation of China [81172186]
- Natural Science Foundation of Hubei Province [2018CFB504, 2017CFB179]
- Guidance Foundation of Renmin Hospital of Wuhan University [RMYD2018M67]
- National Institutes of Health [1R01CA178888]
- Fred and Pamela Buffett Cancer Center [P30CA036727]
- National Science Fund for Excellent Young Scholars [81722007]
- National Science and Technology Major Project [2017ZX10304402001008]
- Independent Research Project of Wuhan University [2042017kf0196]
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Background. As a key step in enhancing cancer cell invasion and metastasis, epithelial-mesenchymal transition (EMT) plays an important role in colorectal cancer progression. EMT is triggered by a variety of signaling pathways, among which the transforming growth factor beta (TGF-beta) signaling pathway has been implicated as a primary inducer. Accumulating evidence demonstrates that MnTE-2-PyP (chemical name: manganese(III) meso-tetralcis-(N-ethylpyridinium-2-yl), a superoxide dismutase (SOD) mimetic, inhibits TGF-beta signaling; however, its ability to inhibit TGF-beta-induced EMT in colorectal cancer has not yet been explored. Methods. To verify our hypothesis that MnTE-2-PyP attenuates TGF-beta-induced EMT, human colorectal cancer cells were treated with TGF-beta in the presence or absence of MnTE-2-PyP. Cells were analyzed by several techniques including western blotting, real-time quantitative PCR, transwell assay, and wound healing assay. Results. MnTE-2-PyP reverses cell phenotypes induced by TGF-beta in colon cancer cells. MnTE-2-PyP treatment significantly reduced the expression of mesenchymal markers but maintained epithelial marker expression. Mechanistically, MnTE-2-PyP suppressed the phosphorylated Smad2/3 protein levels induced by TGF-beta in SW480 cells, but MnTE-2-PyP failed to suppress TGF-beta-induced Slug and Snail expression in colorectal cells. Furthermore, MnTE-2-PyP effectively suppressed TGF-beta-mediated cell migration and invasion and the expression of matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9) in colorectal cells. Conclusion. Taken together, we provide an in-depth mechanism by which MnTE-2-PyP inhibits colorectal cancer progression, supporting an important role for MnTE-2-PyP as an effective and innovative antitumor agent to enhance treatment outcomes in colorectal cancer.
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